ONTzelena kurzIV114A 89539aca-723e-4f70-bfda-e24413cbb4ab FAQ28044 1 Oxford _ NANOPORE Technologies Run Info Host Name Position Experiment Name Sample ID Run ID Acquisition ID(s) How Cell Id Start Time Run Length navsteva2.ibp.cz (localhost) MN34986 ONTzelena kurzIV114A 89539aca-723e-4f70-bfda-e24413cbb4ab 98cd5ca636642d22908c077bd3b5671bb0ad2063, Ib933ce34dd59d8322ebf7b5cd585e8d89e63a77 FAQ28044 October 27, 15:22 3d Oh lm Run Summary Reads Generated Estimated Bases 1.45 M 6.57 Gb Run Parameters Flow Cell Type Kit Initial bias voltage FAST5 output FASTQ output BAM output Bulk file output Active channel selection Basecalling Specified run length FAST5 reads per file FAST5 output options Mux scan period Reserved pores FLO-MIN106 SQK-LSK109 -180 mV Enabled Disabled Disabled Disabled Enabled Disabled 72 hours 4000 vbz_compress,fastq,raw 1 hour 30 minutes 0 % Versions MnKNOW MnKNOW Core Bream Guppy 21.06.13 4.3.12 6.2.6 5.0.11 ONTzelena kurzIV114A 89539aca-723e-4f70-bfda-e24413cbb4ab FAQ28044 Cumulative Output Reads 2 ONTzelena kurzIV114A 89539aca-723e-4f70-bfda-e24413cbb4ab FAQ28044 Read Length Histogram Estimated Bases - Outliers Discarded Estimated N50: 7.96 kb 3 6 Gb-i 5 Gb-I w 4 Gb- 3 Gb- E -»—' w LU 2 Gb- 1 Gb-\ Ob J*-- 16.4 kb 164 kb 311 kb 459 kb 606 kb 754 kb 901 kb 1.05 Mb Estimated read length ONTzelena kurzIV114A 89539aca-723e-4f70-bfda-e24413cbb4ab FAQ28044 Read Length Histogram Estimated Bases Estimated N50: 7.96 kb 4 6 Gb-i 5 Gb-I w 4 Gb- 3 Gb- E -»—' w LU 2 Gb- 1 Gb-\ Ob J*-- 16.4 kb 164 kb 311 kb 459 kb 606 kb 754 kb 901 kb 1.05 Mb Estimated read length ONTzelena kurzIV114A 89539aca-723e-4f70-bfda-e24413cbb4ab FAQ28044 Duty Time Grouped Sequencing Pore Recovering Inactive Unclassified Duty time Categorised • Strand Adapter Single Pore Unavailable Active Feedback No Pore From Scan Out Of Range 2 Possible Multiple Saturated Out Of Range 1 Zero Channel Disabled Unclassified Unclassified_following_reset Pending_manual_reset Pending_mux_change Pending Reselection ONTzelena kurzIV114A 89539aca-723e-4f70-bfda-e24413cbb4ab FAQ28044 Mux Scan Grouped 0 Active Q Inactive 6 ONTzelena kurzIV114A 89539aca-723e-4f70-bfda-e24413cbb4ab FAQ28044 Temperature History 7 Heatsink temperature I I Target temperature 36°C- 33°C- 30°C- 27°C- 24°C- 21 °C-20°C- Om 11 h 40m 23h 20m 1d 11 h Time 1d 22h 2d 10h 2d 22h Bias Voltage History -250 mV-i -200 mV- Voltage CD CO -I—' -100 mV 0 mV 100 mV-120 mV- 13h 53m 1d3h 1d 17h 2d 7h 2d 21 h Time ONTzelena kurzIV114A 89539aca-723e-4f70-bfda-e24413cbb4ab FAQ28044 Disk Write Performance 0 Written Q Produced 0 Held in memory 8 ONTzelena kurzIV114A 89539aca-723e-4f70-bfda-e24413cbb4ab FAQ28044 Run Debug Messages • The sequencing run has finished, but basecalling may continue October 30,15:24 • Mux scan for flow cell FAQ28044 has found a total of 18 pores. 18 pores available for immediate sequencing October 30, 14:36 • Performing Mux Scan October 30,14:34 • Mux scan for flow cell FAQ28044 has found a total of 23 pores. 23 pores available for immediate sequencing October 30, 13:03 • Performing Mux Scan October 30,13:01 • Mux scan for flow cell FAQ28044 has found a total of 24 pores. 24 pores available for immediate sequencing October 30, 11:31 • Performing MUX Scan October 30, 11:28 • Mux scan for flow cell FAQ28044 has found a total of 26 pores. 24 pores available for immediate sequencing October 30, 09:58 • Performing Mux Scan October 30,09:56 • Mux scan for flow cell FAQ28044 has found a total of 29 pores. 28 pores available for immediate sequencing October 30, 08:26 • Performing Mux Scan October 30,08:23 • Mux scan for flow cell FAQ28044 has found a total of 22 pores. 22 pores available for immediate sequencing October 30, 06:53 • Performing Mux Scan October 30,06:51 • Mux scan for flow cell FAQ28044 has found a total of 27 pores. 25 pores available for immediate sequencing October 30, 05:21 • Performing Mux Scan October 30,05:18 • Mux scan for flow cell FAQ28044 has found a total of 27 pores. 26 pores available for immediate sequencing October 30, 03:48 • Performing Mux Scan October 30,03:46 • Mux scan for flow cell FAQ28044 has found a total of 30 pores. 30 pores available for immediate sequencing October 30, 02:16 • Performing Mux Scan October 30,02:13 • Mux scan for flow cell FAQ28044 has found a total of 39 pores. 39 pores available for immediate sequencing October 30, 00:43 • Performing Mux Scan October 30,00:41 • Mux scan for flow cell FAQ28044 has found a total of 39 pores. 39 pores available for immediate sequencing October 29, 23:11 • Performing Mux Scan October 29,23:08 • Mux scan for flow cell FAQ28044 has found a total of 39 pores. 37 pores available for immediate sequencing October 29, 21:38 • Performing Mux Scan October 29,21:35 • Mux scan for flow cell FAQ28044 has found a total of 46 pores. 46 pores available for immediate sequencing October 29, 20:05 • Performing Mux Scan October 29,20:03 • Mux scan for flow cell FAQ28044 has found a total of 42 pores. 41 pores available for immediate sequencing October 29, 18:33 • Performing Mux Scan October 29,18:30 • Mux scan for flow cell FAQ28044 has found a total of 47 pores. 44 pores available for immediate sequencing October 29, 17:00 • Performing MUX Scan October 29, 16:58 • Mux scan for flow cell FAQ28044 has found a total of 53 pores. 47 pores available for immediate sequencing October 29, 15:28 • Performing Mux Scan October 29,15:25 Mux scan for flow cell FAQ28044 has found a total of 60 pores. 55 pores available for immediate sequencing October 29, 13:55 Performing Mux Scan October 29,13:53 Mux scan for flow cell FAQ28044 has found a total of 97 pores. 88 pores available for immediate sequencing October 29, 12:23 Performing Mux Scan October 29,12:20 Mux scan for flow cell FAQ28044 has found a total of 80 pores. 75 pores available for immediate sequencing October 29, 10:50 Performing Mux Scan October 29,10:48 Mux scan for flow cell FAQ28044 has found a total of 79 pores. 74 pores available for immediate sequencing October 29, 09:17 Performing Mux Scan October 29,09:15 Mux scan for flow cell FAQ28044 has found a total of 73 pores. 66 pores available for immediate sequencing October 29, 07:45 Performing Mux Scan October 29,07:42 Mux scan for flow cell FAQ28044 has found a total of 60 pores. 53 pores available for immediate sequencing October 29, 06:12 Performing Mux Scan October 29,06:10 Mux scan for flow cell FAQ28044 has found a total of 60 pores. 56 pores available for immediate sequencing October 29, 04:40 Performing Mux Scan October 29,04:37 Mux scan for flow cell FAQ28044 has found a total of 56 pores. 53 pores available for immediate sequencing October 29, 03:07 Performing Mux Scan October 29,03:04 Mux scan for flow cell FAQ28044 has found a total of 73 pores. 71 pores available for immediate sequencing October 29, 01:34 Performing Mux Scan October 29,01:32 Mux scan for flow cell FAQ28044 has found a total of 58 pores. 58 pores available for immediate sequencing October 29, 00:02 Performing Mux Scan October 28,23:59 Mux scan for flow cell FAQ28044 has found a total of 81 pores. 77 pores available for immediate sequencing October 28, 22:29 Performing Mux Scan October 28,22:27 Mux scan for flow cell FAQ28044 has found a total of 71 pores. 66 pores available for immediate sequencing October 28, 20:57 Performing Mux Scan October 28,20:54 Mux scan for flow cell FAQ28044 has found a total of 82 pores. 75 pores available for immediate sequencing October 28, 19:24 Performing Mux Scan October 28,19:22 Mux scan for flow cell FAQ28044 has found a total of 118 pores. 104 pores available for immediate sequencing October 28,17:51 Performing Mux Scan October 28,17:49 Mux scan for flow cell FAQ28044 has found a total of 132 pores. 107 pores available for immediate sequencing October 28,16:18 Performing Mux Scan October 28,16:16 Mux scan for flow cell FAQ28044 has found a total of 121 pores. 99 pores available for immediate sequencing October 28,14:45 Performing Mux Scan October 28,14:43 Mux scan for flow cell FAQ28044 has found a total of 148 pores. 117 pores available for immediate sequencing October 28,13:12 Performing Mux Scan October 28,13:10 Mux scan for flow cell FAQ28044 has found a total of 170 pores. 126 pores available for immediate sequencing October 28,11:40 • Performing MUX Scan October 28,11:37 • Mux scan for flow cell FAQ28044 has found a total of 204 pores. 145 pores available for immediate sequencing October 28,10:06 • Performing MUX Scan October 28,10:04 • Mux scan for flow cell FAQ28044 has found a total of 232 pores. 159 pores available for immediate sequencing October 28,08:33 • Performing Mux Scan October 28,08:31 • Mux scan for flow cell FAQ28044 has found a total of 280 pores. 181 pores available for immediate sequencing October 28,07:00 • Performing Mux Scan October 28,06:58 • Mux scan for flow cell FAQ28044 has found a total of 337 pores. 210 pores available for immediate sequencing October 28,05:27 • Performing Mux Scan October 28,05:24 • Mux scan for flow cell FAQ28044 has found a total of 419 pores. 250 pores available for immediate sequencing October 28,03:54 • Performing Mux Scan October 28,03:51 • Mux scan for flow cell FAQ28044 has found a total of 491 pores. 276 pores available for immediate sequencing October 28,02:21 • Performing Mux Scan October 28,02:18 • Mux scan for flow cell FAQ28044 has found a total of 569 pores. 310 pores available for immediate sequencing October 28,00:47 • Performing Mux Scan October 28,00:45 • Mux scan for flow cell FAQ28044 has found a total of 669 pores. 360 pores available for immediate sequencing October 27,23:14 • Performing Mux Scan October 27,23:11 • Mux scan for flow cell FAQ28044 has found a total of 786 pores. 394 pores available for immediate sequencing October 27,21:40 • Performing Mux Scan October 27,21:38 • Mux scan for flow cell FAQ28044 has found a total of 912 pores. 424 pores available for immediate sequencing October 27,20:07 • Performing Mux Scan October 27,20:04 • Mux scan for flow cell FAQ28044 has found a total of 1047 pores. 450 pores available for immediate sequencing October 27,18:33 • Performing MUX Scan October 27,18:31 ► Disk usage alert - you only have 392 GB of space free, which is insufficient for the run. Please free up some space, otherwise your run will stop in approximately Id 23h 53m. October 27,17:16 • Mux scan for flow cell FAQ28044 has found a total of 1158 pores. 463 pores available for immediate sequencing October 27,17:00 • Performing Mux Scan October 27,16:57 • Mux scan for flow cell FAQ28044 has found a total of 1283 pores. 493 pores available for immediate sequencing October 27,15:26 • Performing Mux Scan October 27,15:24 • Starting sequencing procedure October 27,15:24 • Waiting up to 300 seconds for temperature to stabilise at 34.0°C October 27,15:22