DNA Repair and Genomic Instability

                    Jaký mechanismus se podílí na opravě dvouřetězcových zlomů?

                                       Why do we study this?



                                 DNA damage in human cell per day:



                                        DNA Repair Pathways

                           Repair by Direct reversal: photoreactivation

                                     Excision Repair Pathways

                                E. coli Base Excision Repair (BER)

                                    Nucleotide Excision Repair

                                     Genetics of NER in Humans

                                       Xeroderma Pigmentosum

                                     Genetics of NER in Humans

                                        DNA Mismatch Repair





                               Recombinational DNA Repair Mechanisms





                                         Cross-link repair

Model for the mechanism of DNA ICL repair in mammalian cells.









                                         Cross-link repair

                                         What do we study?

                                  DNA double-strand breaks (DSB)

                                 Failure to properly process DSBs



                                          End processing

                                          Homology search

                                       DNA repair synthesis

                                            Resolution

                                             Ligation

                                       How do we study this?

                                            Examples -

                                   Regulation of recombination?

                                    Presynaptic Rad51 filament

                              Positive regulation - Mediator proteins



                                            DNA binding

                                     Function of Rad52 protein







                                       EM of Rad51 filaments