BIOCHEMISTRY  II                 LABORATORY CLASSES                              SPRING 2013


Dentistry


ZLBC041c



Week

Date

 Experiments



       4


   13.3.


Directions for laboratory work. Handling biological material.

Introduction to the laboratory experiments. Investigation of glucose metabolism

1.1                Importance of laboratory examinations

1.1.1     Handling biological material, blood collection

1.2                Investigation of glucose metabolism

1.2.1        Enzymatic determination of plasma glucose

1.2.2        Determination of glycaemia by personal glucometer

1.2.3        Oral glucose tolerance test (oGTT)

1.2.4        Detection and determination of glucose in urine

1.2.5      Detection of ketone bodies in urine



        9


   17.4.



Investigation of  hard tissues

2.1           Determination of calcium in serum

2.2           Determination of inorganic phosphate in serum

2.3           Determination of  total ALP catalytic concentration and bone ALP  isoform
              catalytic concentration

2.4           Electrophoresis of ALP isoforms

2.5         Properties of hydroxyapatite



       13


    15.5.


Investigation of saliva

3.1           Investigation of salivary secretion rate

3.2          Estimation of salivary buffering capacity

3.3.         Detection of selected organic compounds in saliva

3.4.         Detection of  a-amylase in saliva and its substrate specifity

3.5.         Determination of selected enzymes catalytic activity in saliva



    14 -15


20 5. – 31.5.



Compensatory lessons. Credits.




Recommended literature:

Biochemistry-Practicals: Information system of MU  (is.muni.cz) - Study materials



Conditions for giving the course-unit credit
  * Full (100%) attendance in labs is the principal condition.

If any absence, it must be apologized through Department of Study Affairs up to five days.

If apology is recorded in Information System (IS), then student is allowed to make up the absence
according to teacher's instructions.
  * Presentation of all lab reports to the teacher


Deadline for any issue (making up all missing and justified lessons, completion of all lab reports
and handing them to the teacher)  is 14. 6. 2013.


Students that will not meet this requirement will not be given the course-unit credit.

Obtaining of course-unit credits of practices is the pre-requisite for registration to the
examination of Biochemistry II.


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