Lesson 3 Routine chemistry analysers Read the text ignoring the gaps and choose the correct answers below: Routine chemistry analysers are ____(1) workhorse instruments of the modern chemistry laboratory. Typically, these instruments perform 80 % or more of ____(2) volume of chemistry testing. ____(3) main properties of automated chemistry analysers (ACA): Availability and cost of instrument interface to the laboratory information system (LIS) Sampling features: Include primary tube sampling, bar code identification, ability of instruments to accept different tube sizes, clot detection, short sample detection, and immediate interrupt Throughput: number of samples and test per hour Menu: test menu and available open channels Easy to use: Includes training, maintenance, and operation On-board quality control software All main-line chemistry analysers offer ___(4) random-access testing; in simple terms, multiple tests can be performed simultaneously and continuously. This contrasts with ___(5) batch-mode instruments that perform single tests on a batch of samples that are loaded on the instrument. Some analysers are so-called open systems that can use ____ (6) reagents from the instrument manufacturer or from alternative vendors. Other instruments are ____ (7) closed systems that can use only reagents supplied by ___(8) manufacturer. These systems are often easier to operate and maintain than open systems. Many instruments have both open and closed channels, allowing ____ (9) some flexibility in the use of reagents. All automatic chemistry analysers use internal automation and robotics for handling specimens and reagents on ___ (10) analyser. 1 Routine chemistry analysers a do the most of chemistry testing b are the most modern instruments in laboratories c did the most of chemistry testing 2 Instrument interface to the LIS a makes ACAs very difficult to use in our LIS b is one of disadvantages of ACA, because it makes them relatively expensive c is one of advantages of ACA, because it makes them relatively cheap 3 Throughput tells us a how many samples were tested b how many samples were tested in a given time period c how many samples we must test 4 In random-access testing, a we can only test samples one at a time b we can test many samples at the same time c we can test only one sample at a time 5 Open systems are those systems where you can use a all reagents from one vendor b all reagents from different vendors c only reagents from the manufacturer 1 Vocabulary warm-up: Can you match the words on the left with their equivalents? 1 ptroerpy a detekce nedostatku vzorku 2 itneracfe b přístroje v dávkovém režimu 3 sgapmlin features c vlastnost 4 lcot tdeection d okamžité přerušení 5 hsort msaple tdeection e charakteristiky dávkování 6 iemmdiate niterurpt f detekce sraženiny 7 trhoughutp g průchodnost, výkonnost 8 on-bdaro h přístroje s náhodným přístupem 9 tbach-mdoe mentsinstru i rozhraní, propojení 10 rdanom-casces mentsinstru j v přístroji, „na palubě“ 2 These definition went a bit wrong. Can you correct them? 1. Bichromatic measurement _________________________ Is run to detect systematic result errors. (See also pre-control, precision control, and quality control.) Numbers H A H 2. Label _______________________ Any substance that stimulates the production of antibodies and combines specifically with corresponding antibodies. 3. Accuracy control _______________________________ Calculation of the absorbance at the primary wavelength minus the absorbance of the reference wavelength. 4. Relative light unit ________________________ Consumable components that must be discarded after use, such as matrix cells. 5. Disposable ________________________ A substance coupled to an antigen or antibody that provides the measurement signal for an immunoassay. 6. Order list monoclonal antibody________________________________ Antibody produced through the fusion of a myeloma cell and a B lymphocyte. Antibodies produced by a single fusion event have identical structure, affinity, and specificity for a given antigen. 7. Antigen __________________________________ Counts generated when the photomultiplier detects light during the chemiluminescence reaction. Abbreviation: RLU. 8. Monoclonal antibody order list______________________________ The screen or report that displays the list of assays waiting to be run.