EIA / MEIA Worksheet

1 Vocabulary warm-up

1 microparticle                                     vázat se

2 coated                                              skleněná vlákna

3 capture molecule                               zvýšit/snížit

4 surface area                                      promytí cely

5 reaction vessel                                  značený

6 matrix cell                                         rychlost

7 glass fibres                                        odstranit

8 binding                                              zadržet

9 cause                                                mikročástice

10 retain                                              povrch

11 labelled                                           reakční nádobka

12 bind                                                způsobit, zapříčinit

13 cell wash                                         navázání

14 remove                                           potažený

15 rate                                                 matricová buňka

16 increase/decrease                            záchytová molekula


2 Microtitre plates

Read and fill the gaps with words made from the words in brackets.

A microtitre plate or microplate or microwell plate or multiwall, is a flat plate with multiple
"wells" used as small test tubes. The microplate has become a standard tool in ______ (ANALYSIS)
research and clinical ________ (DIAGNOSIS) testing laboratories. A very common usage is in the
enzyme-linked immunosorbent assay (ELISA), the basis of most modern ______ (MEDICINE) diagnostic
testing in humans and animals.

Each well of a microplate ________ (TYPICAL) contains between tens of nanolitres to several
millilitres of liquid. They can also be used to store dry powder or as racks to support glass tube
inserts. Wells can be _______ (CIRCLE) or square. Today, there are microplates for nearly every
_________ (APPLY) in life science research which involves filtration, ________ (SEPARATE), optical
detection, _________ (STORE), reaction mixing or cell culture.

3 Listening

A Listen and complete the gaps

1 the EIA can be done in a multi-well microtiter plate or any other solid adherent ___________

2 the plate is prepared to perform a particular assay by _________ the wells with antibodies that
bind to the antigen of interest

3 then the _________ are filled with the clinical sample which could be a sample of serum,
respiratory secretions, cerebrospinal fluid, urine or some other body fluid

4 if the antigen is present in the sample, it will ___________ to the fixed antibodies

5 the wells are then washed out to remove any of the unattached __________, leaving the antigen of
interest stuck to the wells

6 the second _____________, directed against another epitope on the target antigen is added

7 these antibodies are conjugated covalently to an ________________

8 they bind to the antigen which is fixed in the well and this provides a second level of
___________ for the assay

9 the wells are washed again to remove any ______________ antibodies

10 a ____________ of colorigenic enzyme substrate is added


B Find words for the following definitions:

1 not soft, hard

2 determination of the amount of a particular constituent of a mixture

3 take away

4 joined together

5 causing a substance to change colour

4 Describing the assay

A Put the pictures in the correct order and label them with the short texts.

B Describe the whole procedure using the key terms (purpose – equipment – steps – result and
conclusion).

5 Grammar – Passive voice