CYTOLOGY Nucleus, nucleolus Organelles Inclusions Cytoskeleton Cell surfaces Intercellular junctions Nuclear envelope: -outer membrane -inner membrane -nuclear pores -perinuclear space Chromatin: -hetrochromatin -euchromatin Nucleolus p. 2 - nucleus p. 3 p. 4 p. 5 - nucleolus Nuclear organizer Pars fibrosa Pars granulosa Membranes: -outer -inner + cristae Matrix Mitochondrial bodies Mitochondrial ribosomes p. 8 - mitochondrion Cisternae Vesicles Vacuoles Cis face Trans face p.9 – Golgi apparatus Lysosomes -primary -secondary – phagosomes – autophagic vacuoles -residual bodies p. 12 - secondar lysosome p. 13 - autophagic vacuole p.14 - rough endoplasmic reticulum Cisternae of RER p.23 - smooth endoplasmic reticulum Tubules and vesicles of SER p. 17 - peroxisome nucleoid crystaloid p.18 - peroxisome p.20 – centriole (cross section) 9 triplets of microtubules p.21 – centriole (longit. section) p.22 - glycogen (-granules) p.14 - glycogen (-granules) p.23 - lipid droplets p.26 - intercellular junctions ZO ZA MA p.24 – macula adherens - desmosome p.25 - nexus p.27 – microvilli p.28 - brush border p.29 - kinocilium Axoneme – complex of 9 dublets + 1 central pair of microtubules p.30 – kinocilia, microvilli p.31 - flagellum p.49 – basal labyrinth nuclear pore nucleolus endoplasmic reticulum ribosomes inner membrane outer membrane perinuclear space NUCLEAR ENVELOPE heterochromatin NUCLEUS ( Atlas EM: pp. 1, 2, 3, 4, 21, 33, 40, 50) euchromatin How to draw pictures from EM atlas? Mitochondrion (Atlas EM: pp. 8, 10, 18, 22, 23, 49) outer membrane inner membrane cristae mitochondrial bodies (Ca2+) mitochondrial ribosomes vesicles and tubules of SER cisternae of RER with ribosomes Golgi apparatus (GA) vesicles cisternae vacuoles Ribosomes (Ri) • • • • • • free Ri poly(ribo)some ribosomes attached to fiber of mRNA mRNA