Warning
•please, do not manipulate the microscope,
•they are prepared for your study of blood
smears after presentation,
•you will get instructions, how to study
blood smears.
BLOOD, HEMATOPOIESIS
• Composition of blood.
• Blood corpuscles.
• Development of blood /hematopoiesis/.
Blood hematocrit
♂ 42 – 52 %
♀ 37 – 47 %
55 % plasma
90 % H2O
7 % plasma proteins
3 % -AAs, saccharids, lipids
-hormones
-electrolytes
45 % blood corpuscles
LEUKOCYTES
GRANULOCYTES
(polymorphonuclears)
AGRANULOCYTES
(mononuclears)
neutrophilic 10 - 12 m
eosinophilic 12 - 14 m
basophilic 8 - 10 m
lymphocytes 6 - 8 / 10 -12 m
monocytes 12-20 m
THROMBOCYTES
ERYTHROCYTES
BLOOD CORPUSCLES
7,2 - 7,6 m
2 – 4 m
3.5-5.5 million/μl
4.500-11.000/μl
150.000-400.000/μl
ERYTHROCYTES
polyglobulia - polycythaemia / anemia / anisocytosis / poikilocytosis
Eos
Ery
Ery
ERYTHROCYTES
7.2-7.6 µm
ERYTHROCYTE
RETICULOCYTE
substantia
reticulofilamentosa
Neu-B
Neu-S
NEUTROPHILIC GRANULOCYTES
- bands, segments -
NEUTROPHILIC GRANULOCYTE
„BAND“
NEUTROPHILIC GRANULOCYTE
„SEGMENT“
Eos
Neu
EOSINOPHILIC GRANULOCYTE
EOSINOPHILIC GRANULOCYTE
EOSINOPHILIC GRANULOCYTE
BASOPHILIC GRANULOCYTE
LYMPHOCYTE
LYMPHOCYTE
Mono
Ly
MONOCYTE and LYMPHOCYTE
Mono
Neu
MONOCYTE and „NEUTROPHIL“
phagosomes
MACROPHAGE
Ly
Neu
THROMBOCYTES
BLOOD PLATELETS
ERY
THROMBOCYTE
BLOOD
Slide
Peripheral blood smear, panoptic staining (method
of Pappenheim), immersion oil, magnif. 1000x
How to study blood smear in LM?
• Lens of immersion objective /magnifying 100x/ is
immersed into drop of immersion oil and blood smear
is prepared for study.
• Swich on the microscope and check the picture in
the microscope.
• If the image is not sharp, focus it using fine adjustment
knob! If it is not possible, contact your teacher.