The connective tissue proper
Connective tissue cells
Extracellular matrix - fibers
- ground substance
Classification of connective tissue
Fibroblast + collagen fibrils
Fibroblast – synthetically active cell
GA
GER
CMF
Pigment cells
melanosomes
Adipocytes
Plasma cell
GER
Mast cell
Dense secretory granules
http://www.gwc.maricopa.edu
Wharton´s jelly – Funiculus umbilicalis
Nuclei of fibrocytes - violet Collagen fibers - blue
Loose connective tissue - Esophagus
Loose connective tissue – mucosa, submucosa
Dense connective tissue regularly arranged - Tendon
Dense connective tissue irregularly arranged - Sclera
http://jmugica2003.blogspot.cz/
Elastic membranes - Aorta
Reticular tissue – Lien (impregnation)
http://quizlet.com
Reticular connective tissue - intestinum crassum
(HE)
Light microscope
 Eyepieces
 Objective lens
 Stage with speciment
holder
 On/Off
 Light control
 Condenser and Iris
aperture
 Stage controls
 Coarse focus
 Fine focus
 Light source with
diaphragm
• Turn on the light.
• Start with the 4x objective.
• Put the slide on the stage – cover glass must be above
• Look through the scope and focus. Use the coarse focus knob at first, until
the image is more or less in focus; then switch to the fine focus.
• Adjust the light. Not too bright, not too dim.
• Adjust the oculars.
• Switch to the 10x objective. A slight adjustment with the fine focus knob
should get it just right. If you lose the focus and can't see your specimen at
all, go back to the 4x and start again.
• Switch to the 40x objective if you want to see more detail. Don't use the
100x!
• When you want to look at a new slide, switch back to the 4x before
changing slides.
• Only one slide is out of the box at the moment! Do not remove more!
• When you're done with the scope, switch to the 4x and turn the light all the
way down before turning it off.
• At the end of lesson, the box with slides is checked in your presence
before you leave your place
Instructions
Various shapes on different sections of
tubular structures
Cross section Longitudinal
section
Oblique section
Connective tissue proper:
Slides:
Wharton´s jelly (99. Funiculus umbilicalis)
Loose (areolar) connective tissue (11. Esophagus)
Dense connective tissue - irregular (89. Posterior part of
the eye)
Reticular tissue (68. Lien – impregnation)
Elastic membranes (62. Aorta)
Atlas EM:
Fibroblast (42)
Plasma cell (43)
Mast cell (44)
Collagen fibers (42, 44)