Flow cytometry


Flow cytometry is a technology that measures and then analyses multiple physical characteristics of
single p_ _ _ _ _ _ _ _s, usually cells, as they flow in a f_ _ _d stream through a b_ _m of light.
The properties measured include a particles’ relative size, relative g_ _ _ _ _ _ _ _ _y or
internal c_ _ _ _ _ _ _ _y, and relative f_ _ _ _ _ _ _ _ _ _e intensity. These characteristics are
determined using an optical-to-electronic coupling system. This system records how the cell or
particle s_ _ _ _ _ _s laser light and e_ _ _s fluorescence.

A flow cytometer is made up of three main systems: fluidics, optics and electronics.

The fluidics system transports particles in a stream to the laser beam for interrogation.

The optics system consists of lasers to i_ _ _ _ _ _ _ _e the particles in the sample stream and
optical filters to direct the resulting light signals to the a_ _ _ _ _ _ _ _ _e detectors.

The electronics system c_ _ _ _ _ _s the detected light signals into electronic signals. These
signals can then be p_ _ _ _ _ _ _d by the computer.

In the flow cytometer, particles are carried to the laser intercept in a fluid stream. Any
suspended particle or cell from 0.2-150 micrometers in size is suitable for analysis. The portion
of the fluid stream where particles are located is called the sample core. When particles pass
through the laser intercept, they scatter laser light. Any fluorescent molecules present on the
particle fluoresce. The scattered and fluorescent light is collected by appropriately positioned l_
_ _ _s. A combination of beam splitters and filters brings the scattered and fluorescent light to
the appropriate detectors. The detectors produce electronic signals. These signals correspond with
the optical signals striking the detectors.


Questions:

Source: http://www.d.umn.edu/~biomed/flowcytometry/introflowcytometry.pdf


Vocabulary warm-up

1 Particles                          příslušný

2 Fluid                                 vydávat, vyzařovat

3 Beam                                čočky

4 Granularity                     optická lavice/soustava

5 Complexity                    zpracovat

6 Fluorescence                granularita

7 Emit                                  částice

8 Scatter                             paprsek

9 Illuminate                      složení (buňky)

10 Appropriate                kapalina

11 Convert                         fluorescence

12 Process                          laločnatost

13 Lenses                           rozptýlit

14 Lobularity                     osvítit, ozářit

15 Optical bench             přeměnit

Source: Dastych M., English for Laboratory Technicians, Brno – MU, 2008.

Grammar point

Past perfect (předminulý čas). Source: Murphy, R., English Grammar in Use, Cambridge University
Press, 2012.