Name: Numer: Study group:
This text was supported by project FRMU no. MUNI/FR/1552/2015 (Faculty of Medicine, Masaryk University).
Protocol
Spirometry
Methods
Equipment
Spirometer for Power Lab, WinSpiro spirometer, filter, nose clip, sterile mouthpieces,
disposable mouthpieces, Powerlab system
Procedure
PowerLab system:
1. Start program SPIROMETRY by double-clicking on the icon. Put the spirometric device
on the table, in the 1st channel Flow choose Spirometry Pod item from the pop-up menu
and press buttons Zero and then Ok.
2. Examined person sits 2. on a chair with his/her back turned to PC monitor in order not to
see the record. The nose clip is fixed on the nose and spirometer device with filter and
sterile mouthpiece is placed into the mouth of experimental subject. The sensor must be
held in horizontal position; the two white plastic tubes should be pointing upwards.
3. Click on the Start button. The 1st channel shows the speed of airflow through the sensor
in ml/s, the 2nd channel shows the integral of flow, which is volume, in litres. If the
recording is upside-down (inspiration down, expiration up), choose Spirometry Pod (in
the 1st channel Flow), then choose Invert and then press Ok.
4. Record following situations:
 Resting breathing for approx. 1 min and 20 seconds
 4 resting breathing cycles followed by 1 breathing cycle with maximal
inspiration, 4 resting breathing cycles and 1 breathing cycle with maximal
expiration (and once again the whole procedure, without an interruption of
recording)
 4 resting breathing cycles, then 1 breathing cycle with maximal inspiration
followed by maximal expiration (expire as much volume of the air as you can,
speed does not matter in this task) and then 4 resting breathing cycles.
 Hyperventilation for about 15 sec
5. In case the record is significantly shifted upwards or downwards, correct it: in the 2nd
channel Volume mark resting breathing in the course of minimally 1 minute and choose
Spirometry volume and Apply volume correction. Then press Ok.
6. Save the record under the name “spirometryXY“, where XY are initials of the name of
the experimental subject, file type is Data Chart File (*.adicht).
7. In the 2nd channel Volume measure and calculate parameters given in Table 13.
Measured values appear in miniwindow Volume, time difference in mini-window
Rate/Time.
8. In the 2nd channel Volume choose the whole record. Zoom it by pressing Zoom View, in
File choose Print Zoom View. Diminish the record by means of mouse to one third of
original size by moving the picture upwards and print it by pressing Ok.
Name: Numer: Study group:
This text was supported by project FRMU no. MUNI/FR/1552/2015 (Faculty of Medicine, Masaryk University).
Spirometer WinSpiro
1. Start the program winspiroPRO by double-clicking the icon.
2. Click on the icon Patient; new window called Patients will open
3. Click on the icon New; window for the input of data about the examined person pops-up
 Click on the icon Auto number; program will now generate ID for examined person
 Fill in the fields: Last name, First Name, Date of birth, choose Gender, from the scrollbar,
choose the Ethnic group to which examined persons belongs (Caucasian is preset
since this is the ethnic group where most white people from Europe belongs)
 After filling in all the data, click the button Save and the button Go to visit; window
Patients may be closed now
4. New window called Insert new visit card appears.
 Into the field Visit card valid from, fill today´s date
 Field Insert new details should be already chosen and marked with a dot
 Fill in the Height and Weight of the examined person
 Click on the icon Confirm new visit card
5. The active window is now called Clinical file of patient XY, where XY is the name of
examined person
 In the field Visit reason choose Control visit
 Choose, whether the examined person is a smoker, non-smoker or ex-smoker; if
he/she is a smoker, then fill, what does he/she smoke, how long and how much does
he/she smoke.
 Leave the other fields empty. Click on the icon Save and close the Clinical file of
patient XY window
Note: All input data (gender, age, height, weight, smoking status) are used to place the patient into a
group for which there are physiological values saved in the program. Software will further evaluate,
how big is the difference (in percent %) in the results of spirometry of the examined person compared
to population average.
6. Now the main window „WinSpiroPRO 3.6“ should be active. Connect the disposable
mouth piece to the spirometer, fix the nose-clip on the nose of examined person.
Examined person sits with his/her back turned to the PC monitor in order not to see the
recording.
7. Click on the icon FVC (Forced Vital Capacity)
 Two windows called „FVC-PRE“ a „Incentive“ will open; you will work only with the
„FVC-PRE“ window; in the „Incentive“ window, there is only a graphical
visualization of the strength of the expiration (represented by opening and closing of
the curtain, behind which the picture is hidden)
 By clicking Start button, you will start the recording of the Flow-Volume loop
 Examined person will perform 2-3 resting breathing cycles followed by maximal
inspiration and maximal expiration that must be as fast as possible
 After expiration, take the spirometer off the mouth; examined person breaths
peacefully; click on the icon Accept to save the recording; if examined person starts
coughing or anything else happens during the examination, by clicking the
Stop/Delete icon it is possible to delete the recording and start it again using
 the Start icon
 Repeat the examination for 2 more times until you obtain properly performed
Flow‑Volume loop
 After every successful recording of the Flow-Volume loop, click on the icon Accept in
order to see the results
Name: Numer: Study group:
This text was supported by project FRMU no. MUNI/FR/1552/2015 (Faculty of Medicine, Masaryk University).
 From the part “Baseline spirometry” choose “Best values” for FVC, FEV1, PEF,
FEV1/FVC, write them down to your protocol. Also redraw a Flow-Volume loop.
8. Click on the icon VC (Vital Capacity)
 After a sound signal, examined person performs a few resting breathing cycles until
approx. 20th second of the recording
 After the second (long) sound signal, examined person will maximally inspire and
maximally expire (does not matter how fast, the volume matters!) and then breaths
peacefully
 The recording will be stopped automatically after 1 minute
 Repeat the test two more times and write down the values of the lung volumes
obtained from the best measurement
9. Click on the icon MVV (Maximal Minute Ventilation)
 After a sound signal, examined person performs hyperventilation (i.e. maximal
inspiration followed by maximal expiration with the highest possible frequency)
 Recording will be stopped automatically after 15 seconds
 Examined person is breathing peacefully for a few minutes, after calming down,
repeat the test 2 more times
 Write down the value of MMV from the best measurement
10. Click on the icon Results. Into the field Conclusion/Medical report(Spirometry) fill in the
suggested diagnosis (obstructive or restrictive lung disease; normal spirometry finding).
Click on the icon Print, protocol with the results will be generated and it can be printed
now.
Name: Numer: Study group:
This text was supported by project FRMU no. MUNI/FR/1552/2015 (Faculty of Medicine, Masaryk University).
Results
Fill the table.
respiratory
parameter
abbreviation units physiological
values
resting breathing
frequency f breaths/min
tidal volume Vt litre (l)
minute ventilation Vm = f · Vt l/min
Inspiratory reserve
volume
IRV l
inspiratory capacity IC = IRV + Vt l
expiratory reserve
volume
ERV l
expiratory capacity EC = ERV+ Vt l
vital capacity VC l
forced vital capacity FVC l
forced expired
volume in 1 s
FEV1 l
relative forced
expired volume in 1 s
FEV1/FVC
hyperventilation
maximal frequency fmax breaths/min
maximal tidal
volume
maxVt l
maximal minute
ventilation
maxVm = fmax · maxVt l/min
Conclusion
Write into the conclusion whether the measured volumes correspond to physiological values.
Is there any clinically significant respiratory disorder?
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Name: Numer: Study group:
This text was supported by project FRMU no. MUNI/FR/1552/2015 (Faculty of Medicine, Masaryk University).
Protocol
Pneumography
Methods
Equipment
Two respiratory movement sensors, nose clip, sterile mouth piece, Krogh respirometer,
PowerLab system
Procedure
1. Experimental subject is standing with his/her back turned to PC monitor in order not to
see the screen and record. Fix the respiratory belts on chest (1st channel) and abdomen
(2nd channel). They must be fixed tightly enough in order to register the respiratory
movements but not too tightly to prevent them being damaged!!!
2. Start program PNEUMOGRAPHY by double-clicking on the icon.
3. Set the sensitivity of the amplifier for recording of respiratory movements in the 1st and
the 2nd channel. Upper trace corresponds to the recording from the chest; lower trace
corresponds to the recording from the abdomen.
4. Record following situations:
• Resting respiration – record for one minute.
• Respiration after a mild exertion – examined person performs 5 squats. Record at
least 10 breathing cycles immediately thereafter.
• Respiration after an intensive exertion – examined person performs 30 squats.
Record at least 10 breathing cycles immediately thereafter.
• Respiration after re-breathing of the expired air – connect experimental subject to
Krogh respirometer which is filled with 5 litres of oxygen (for details, see practical
exercise No. XXII; briefly: experimental person exhales; after exhaling turn the side
valve on the Krogh respirometer in order to connect experimental person to the tank
with oxygen; after closing the valve, experimental person can inhale and he/she is now
• inhaling the oxygen from the Krogh respirometer). A nose clip prevents mixing of the
respired air with the atmospheric air. Since there is CO2 in the expired air and there is
no CO2 absorber in the Krogh respirometer, its concentration in the respirometer
gradually increases which leads to an increase of pCO2 in the body and to changes in
respiration. After reaching observable changes in respiration (approx. after 2–3 min),
open the side valve on the respirometer (experimental person is now breathing the
atmospheric air). Record at least 20 consecutive breathing cycles and observe how
respiration gradually recovers. For further evaluation, choose only the first six
breathing cycles after disconnection from the respirometer.
5. Insert comments about all performed situations in the record.
6. Save the record under the name „pneumographyXY“, where XY are initials of the name
of the experimental subject, file type is Data Chart File (*.adicht). Do not print the record.
Name: Numer: Study group:
This text was supported by project FRMU no. MUNI/FR/1552/2015 (Faculty of Medicine, Masaryk University).
Evaluation
Choose one of the channels and measure following parameters in 6 chosen breathing cycles
for all situations: duration of inspiration (Ti), duration of expiration (Te), breathing interval
(BI) and amplitude Vt (see figure). Measured values will appear in mini-window Rate/Time.
Write down obtained values in the table.
̅
∑ ( )
√
∑ ( ̅)
√
[( ̅) ( ̅) ( ̅) ]
x: Ti, Te, Vt, BI
̅: mean x
SD: standard deviation
Name: Numer: Study group:
This text was supported by project FRMU no. MUNI/FR/1552/2015 (Faculty of Medicine, Masaryk University).
Results
cycle 1 2 3 4 5 6 mean SD
Resting
respiration
Ti
Te
BI
Vt
After a
mild
exertion
Ti
Te
BI
Vt
After an
intensive
exertion
Ti
Te
BI
Vt
After re-
breathing
of the
expired air
Ti
Te
BI
Vt
Conclusion
Summarise the results of measurement and compare how the measured values of Ti, Te, BI
and Am differ at rest, on a mild exertion and on intensive exertion. Evaluate in which way the
values of Te, BI and Am change when breathing into a bag.
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