Practice no. 13 – Blood and hematopoiesis (deadline 15. 5. 2020)
1. What is the hematocrit, how it can be obtained, and what are the normal values in men
and women?
2. Graphically schematize the normocyte (normal erythrocyte), include the sizes, and define
the terms describing deviations from the norm: anisocytosis and poikilocytosis. Provide
examples of abnormal erythrocytes.
3. How does the osmotic pressure of environment affect morphology of the erythrocyte?
What is the osmolality of blood plasma?
4. Draw in correct size ratio: neutrophilic, basophilic and eosinophilic granulocyte (including
arrangement of nuclear segments and specific granules), lymphocyte, monocyte, and
thrombocyte.
5. Determine normal number of erythrocytes, leukocytes and thrombocytes per volume
unit.
6. Create a table describing differential white blood cell count. For each type of leukocyte
include normal values [in %]. Describe in words the increased and decreased numbers.
7. Create a brief scheme of hematopoiesis. Starting with morphologically distinct precursors
(proerythroblast, myeloblast, megakaryoblast), graphically schematize the structure and
staining of individual stages within a lineage.
8. Explain the terms „substantia reticulofilamentosa“, „enucleation - nuclear extrusion“,
„endomitosis“, „demarcation membrane system (canals)“, „Barr’s body“. Which blood
cells (developmental stages) these terms refer to?
9. What are the stages of embryonic/fetal hematopoiesis? When and where do they take
place?
Recommended study resources:
Presentation from practice
Presentation from lecture
Atlas of Histology