BLOOD
Slide:
Peripheral blood smear, panoptic staining (method of
Pappenheim), immersion oil, magnif. 1000x
How to study the blood smear in LM?
• please, do not manipulate the microscope
• they are prepared for your study of blood smears after presentation
• lens of immersion objective (magnifying 100x) is immersed into drop of
immersion oil and blood smear is prepared for study
• swich on the microscope and check the image in the microscope
• if the image is not sharp, focus it using only the microscrew! If it is not possible,
contact your teacher.
• look for all types of WBCs (just move the table), draw and label them into the
protocols compared to RBC, draw and label blood platelets
• examination of DWCC (after break)
Blood smear - processing
single ery clumps of ery
thin thick
smear smear
How to „count“ leukocytes in blood smear?
The blood smear have to be systematicaly viewed (it avoids
repeatedly „count“ the same cells).
or
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start
Differential white cell count (DWCC) - table
1 2
Neu band
/
Neu
segment
//// // ///
Eos
/
Baso
Ly
// ////
Mono
//
10 10
9 10 results norm
// * 4 %
//// / /// *67 %
/ // 3 %
/ 1 %
/ //// 20 %
5 %
10 10 100 100 %
bands : segments - 4 % : 68 % (1 : 17)
shift to the left
shift to the right
*
Anomalies of DWCC
 
Neutrophils neutrophilic
granulocytosis
neutrophilic
granulocytopenia
Eosinophils eosinophilic
granulocytosis
eosinophilic
granulocytopenia
Basophils basophilic
granulocytosis
basophilic
granulocytopenia
Lymphocytes lymphocytosis lymphocytopenia
Monocytes monocytosis monocytopenia