Pathophysiology of hematopoietic system I– hematological malignancies Assoc. Prof. RNDr. Sabina Ševčíková, PhD. Babak Myeloma Group Department of Pathophysiology I. Hematopoiesis II. Basic overview of blood cells III. Hematological malignancies IV. Survival of patients with hematological malignancies I. Hematopoiesis Hematopoiesis Pinho 2019 process of creation of cell components of blood adult human produces 4 – 5 x 1011 of hematopoietic cells daily highly regulated, highly responsive system Production and destruction of blood • Production of blood • the liver creates protein components of blood • the endocrine glands produce hormones • the GI tract and kidneys maintain water fraction • Destruction of blood • Spleen – destruction of blood cells • Liver – destruction of blood cells, proteins and amino acids collected • Kidneys – proteins collected; amount of water regulated Hematopoietic stem cells - HSC • Multipotent - capable of generating entire hematopoietic system • Embryogenesis - aorto-gonado-mesonephros region, fetal liver • Adults - bone marrow • highly specialized rare cells • self renewal • differentiation into functional progenitors • important for renewal after transplantation, infection, wound • balance between differentiation and self renewal • Intracellular factors • Regulators of transcription and epigenetics, metabolic pathways • Extracellular factors • Humoral and neural signals, signals from the bone marrow niche Pinho 2019 Hematopoietic stem cells - HSC • 1:10 000 cells in the bone marrow • Isolated based on Hoescht dye exclusion, resistance to 5-fluorouracil or ϒ irradiation • Flow-cytometry – lack of CD markers of mature cells, expression of c-Kit (receptor for cytokine stem cell factor) • Reside in specific niche in the bone marrow Tsuruta 2012 Adult bone marrow in homeostasis Pinho 2019 II. Basic overview of blood cells Blood smear Eosinophil Neutrophil Basophil Neutrophil Monocyte Thrombocyte Lymphocyte Erythrocytes • Round, binconcave (larger area for gas exchange) • no cell nucleus or organelles Function • transport of gases that are bound to hemoglobin inside erythrocytes • transport of oxygen from lungs to the tissues, of CO2 from tissues to lungs and out of the body Thrombocytes • small cells, oval shape, survive for four days, do not contain cell nucleus • created by fragmentation of cytoplasm of large cells called megakaryocytes • Function • ability to adhere and congregate • involved in coagulation, every time a blood vessel is injured • involved in the production of the thrombus that protects from large loss of blood Leukocytes • blood cells that are lighter in color and contain nucleus in comparison to erythrocytes • divided based on size, shape of nucleus and function Leukocytes • Function • cells with ability to adhere, perform diapedesis and phagocytosis • part of the immune system • involved in a protective mechanism of the organism • numbers increase in infections and inflammation Lymphocytes • round cells with a small amount of cytoplasm and one round nucleus • two basic groups differing in function • T – lymphocytes (direct destruction) • B – lymphocytes (production of antibodies) • Function • involved in specific immunity of the organism- antigen specific receptors • small fraction of lymphocytes - in peripheral blood, most are in the bone marrow, spleen, lymph nodes • after recognizing a foreigner particle, they start the protective reaction of the organism leading to destruction of the foreign particle B-lymphocytes • Originate and mature in the bone marrow, then migrate to lymph nodes, spleen and intestines • after recognizing an antigen, they turn into plasma cells - production of antibodies (immunoglobulins - Ig) • plasma cells migrate to peripheral blood, intestines, breast milk, tears etc B-lymphocytes – production of antibodies • to recognize and destroy foreign objects in the organism • specific recognition of antigen based on a principle of a lock and key • once an antibody reacts to specific antigen, a cascade is started leading to elimination of that pathogen • Function of antibodies: opsonization, neutralization, complex formation • 5 classes of antibodies: • IgG, IgA, IgM, IgE and IgD Antibodies IgG antibodies are able to get into tissues and are the only ones that can enter the fetus through the placenta. IgA antibodies are produced mainly in the mucous membranes of the intestine and breathing tube and protect the body from microorganisms entering the body IgM antibodies are produced first during infection. They protect the organism within the first few days before other types of antibodies are produced IgE antibodies are produced as a protection against parasites and are involved in allergic reactions IgD antibodies are rare and are involved in histamine release T lymphocytes • Originate in bone marrow, thymus (if no thymus, no mature T cells) • Mature T cells migrate to lymphoid organs, especially lymph nodes, spleen, bone marrow and peripheral blood • Bind antigens using TCR receptors • Unable to produce antibodies • destroy cells that had been attacked by microorganisms • regulate function of other immune cells T – cells are target cells of HIV virus Classes of T cells • Cytotoxic (Tc) • directly kill cells (some viruses are able to survive and duplicate inside cells. Infected cells need to be destroyed so that the infection does not spread) • Helper (Th) • support the function of other cells of the immune system (Tc, B cells, macrophages) HIV • acquired immune deficiencies - immune system effected during the lifetime of an individual • acquired immune deficiency syndrome (AIDS) • HIV infects Th lymphocytes, macrophages and CNS cells • after initial infection, virus survives in the body for several years without any symptoms • then virus replicates - Th cells drastically decrease • insufficient amount of Th cells leads to opportunistic infections (Kaposi sarcoma…) Monocytes • large cells with a round or kidney shaped nucleus • created in the bone marrow, migrate to peripheral blood where they circulate for about 8 hours • then they enter tissues and change into macrophages • Function • monocytes and macrophages are part of the immune system • the basic function of macrophages is the phagocytosis of bacteria, foreigner bodies or dead cells Granulocytes • Polymorphous nucleus - two to five segments • cytotoxic granules in the cytoplasm • Neutrophil - pinkish purple granules • Eosinophil – orange-red granules • Basophils - dark blue granules • Function • granulocytes are part of the non-specific immunity • involved in destruction of bacteria and parasites • Most common type of white blood cells with the shortest half life (12 hrs in blood, 1-2 days in tissues) • Professional phagocytes - inflammation • Function: • Phagocytosis (if opsonization, phagocytosis is easier) • Opsonization – process increasing effectivity of phagocytosis • Chemotaxis – ability to migrate to a place with highest concentration of bacteria • Diapedesis – ability to migrate from peripheral blood into the place of inflammation through the wall of the vein • Perform phagocytosis only once, then they die Neutrophils • weak phagocytes • main function is protection against parasites • Accumulate in places where parasites enter body (lungs, GIT) • Release granules that contain chemicals attacking the parasites • involved in allergic reaction Eosinophils • Least common of all granulocytes and leukocytes • Receptors for IgE on membrane • Their granules contain heparin and histamine- inflammation and allergies • Mast cells – in tissues and connecting tissues • Histamine • Effects muscles, increases permeability of blood vessels • Massive release during allergic reaction Basophils III. Hematological malignancies Important definitions • Incidence - number of new cases of a disease diagnosed each year • Prevalence - a measure of the total number of people in a specific group who have (or had) a certain disease, condition, or risk factor at a specific point in time or during a given period of time. • Overall survival - length of time from either the date of diagnosis or the start of treatment for a disease, such as cancer, that patients diagnosed with the disease are still alive. Important definitions • Remission – a decrease or disappearance of signs and symptoms of cancer, including normalization of lab values (blood count) and imaging methods (X ray, ultrasound, CT) in response to treatment. • Complete remission - The disappearance of all signs of cancer in response to treatment. This does not always mean the cancer has been cured. Also called complete response. • In hematological malignancies (leukemias), the total number of leukemic cells in blood is observed. Partial remission means decrease of leukemic cells by at least 50%. • Relapse - return of a disease or the signs and symptoms of a disease after a period of improvement. Reaching remission does not mean cure as there might be lesions that are impossible to detect and may become the source of new return of the disease. https://www.cancer.gov/publications/dictionaries/cancer-terms/search?contains=false&q=overall+survival Minimal residual disease - MRD • Tumor cells not eradicated by the treatment • Usually results in growth of these cells – resistance to treatment • Emerging component of CR assessment in MM patients • MRD negativity - associated with significantly longer OS in MM patients Paiva et al, 2008; Rawston et al., 2013 Minimal residual disease Diagnosis Treatment Clinical remission Relapse Persistence Cure MRD DETECTION Hematological malignancies Leukemia Lymphoma Multiple myeloma Hematological malignancies Leukemia Lymphoma Multiple myeloma • From Greek – leukos-white, hemos-blood • Symptoms known in the era of Hippokrates (460 - 370 BC) • R. Wirchow described in 1839 – 1845, when microscopy was used • R. Wirchow named leukemia • „Omnis cellula e cellula“ Leukemia Leukemia • heterogeneous group of diseases • most common tumors in children • leukemic cells lose the ability to differentiate, high proliferation potential • two cell populations in the body - mature cells and immature cells = blasts Clinical features • Erythropenia – anemia • Thrombocytopenia – bleeding • Leukocytopenia – infections Prognosis of leukemia Morphology Chromosomal aberrations Age – worse prognosis B cells - worse prognosis Treatment of leukemia • Induction – treatment given with intent to induce complete remission • Consolidation – repetition of induction in a patient with induced complete remission to increase cure rate • Maintenance – long-term, low-dose treatment to delay regrowth of residual tumor cells • radiation and chemotherapy (combination) After chemotherapy • biopsy of bone marrow • further treatment if 5-10% of blasts • bone marrow transplantation Leukemia Acute Chronic Myeloidní Lymfoidní Leukemia Acute Chronic Myeloid Lymphoid Acute leukemia • fast proliferation of immature cells • bone marrow does not produce enough healthy cells • leukemic cells get into peripheral blood and infiltrate other organs (even CNS) • fast treatment needed – „medical emergency“ • most common in children Chronic leukemia • proliferation of relatively mature but abnormal cells • lasts for months or years • treatment not necessary at once in comparison to acute leukemia • mostly in older people ALL – more common in children AML – more common in elderly CLL – most common in adults CML – mostly in adults Hematopoesis Lymphoid leukemia Myeloid leukemia Risk factors for leukemia development • ionizing radiation • chemicals – benzene, cytostatics, alkylators and carcinogens • syndrome: Down (trisomy 21), Klinefelter (47, XXY) • viruses – HTLV-1 causes development of leukemia from T cells in adults • secondary leukemia - common after treatment for other malignancies Acute myeloid leukemia - AML Acute myeloid leukemia - AML • Fatigue, fever, bleeding • accumulation of blasts in bone marrow (> 20 %), bone marrow failure • Blasts in peripheral blood • Differentiation block at various stages of development • Most common leukemia in adults over 65 (80%) • about 20,000 of newly diagnosed patients in a year • Incidence 1.3/100 000 until 65, 12.5/100 000 over 65 • 70% of patients die within one year after diagnosis Auer rods • typical feature of AML • in cytoplasm of myeloblasts • negative prognostic marker • abnormal fusion of primary granules • Identified in 1905 Prognosis of AML Morphology Chromosomal aberrations Age at diagnosis Number of leukocytes at diagnosis FAB classification Classification of AML • 8 subtypes • based on morphology and cytochemistry FAB French American British classification • based on molecules, morphology and clinics WHO classification Subtype FAB Type Morphology Cytogenetic Abnl AML w/o maturation M0 no azurophil granules AML M1 few Aeur rods del(5); del(7); +8 AML w/ differentiation M2 maturation beyond promyelocytes; Auer rods t(8:21) t(6:9) Acute Promyelocytic Leukemia M3 hypergranular promyelocytes; Auer rods t(15:17) Acute Myelomonocytic Leukemia M4 > 20% monocytes; monocytoid cells in blood inv(16) del(16) t(16:16) t(4:11) Acute Monocytic Leukemia M5 monoblastic; promonocytic t(9:11) t(10:11) Acute Erythroleukemia M6 predominance of erythroblasts; dyserythropoiesis Acute Megakaryocytic Leukemia M7 dry' aspirate; biopsy dysplastic with blasts Classification of AML FAB Classification WHO classification Swerdlow 2016 Differences in survival of young and older AML patients • upper graph shows survival of younger patients from 1970 (<60 years) • lower graph shows survival of older patients from 1970 • Kantarjian et al 2015 - MD Anderson Acute promyelocytic leukemia - APL the most malignant human leukemia APL • accumulation of promyelocytes (differentiation stage of granulocytes) • M3 classification based on FAB • treatment commenced immediately – medical emergency • for a diagnosis - detection of translocation necessary • median age at diagnosis 40 - same risk throughout lifetime • 1957 - subtype of leukemia • 1970 – identification of translocation - Dr. J. Rowley Molecular basis of APL • RARα – receptor pro all-trans retinoic acid • PML – promyelocytic gene • Translocation t(15;17) – reciprocal translocation APL treatment APL treatment Crespo-Solis 2016 APL survival Acute lymphoid leukemia - ALL Acute lymphoid leukemia - ALL • malignant transformation and proliferation of lymphoid progenitor in the bone marrow, peripheral blood and extramedullary sites • 80% ALL in children • Incidence 1.6/100 000 (USA) • 2016 - 6590 of newly diagnosed patients, 1400 deaths • bimodal distribution of incidence – children (4 years) and adults (50 years) • In children – survival 90% but only about 30-40% of adults reach long-term remission ALL etiology • significant correlation with Down syndrome, Fanconi anemia, Bloom syndrom, Ataxia Telangiectasia and Nijmegen breakdown syndrome • ionizing radiation, pesticides, smoking • Viruses - Epstein-Barr and HIV • Often de novo • Chromosomal aberrations t(12;21), t(1;19), t(9;22) and aberrations in MLL – not enough for ALL development – unknown origin • Induction (vincristin, corticosteroids, anthracyclins) • Transplantation of bone marrow • Or • Consolidation • Maintenance 2-3 years ALL treatment Terwilliger 2017 Chronic myeloid leukemia - CML Chronic myeloid leukemia - CML first tumor linked to specific translocation between chromosomes 9 and 22 t(9;22) Philadelphia chromosome •1960 – Peter Nowell and David Hungerford described an abnormal chromosome in CML •First genetic cause of tumors •1972 – reason or consequence? Janet Rowley – t(9,22) CML • first tumor linked to specific aberration • CML chromosome described in 1960 in Philadelphia – Philadelphia chromosome • 1972 translocation described t(9;22) (Rowley) • 1983 kinase abl described on chromosome 9 (Heisterkamp) • 1984 bcr region described on chromosome 22 (Groffen) • 1990 bcr-abl reason for CML (Daley) • Bcr-abl- abnormal tyrosin kinase (Lugo, 1990) • Chronic phase, accelerated phase, blast crisis • Very bad prognosis (Less than 3 years) CML • Incidence 1-2/100 000 • 15% newly diagnosed patients with leukemia • 9000/year of new cases in USA • 1000/year die (since Gleevec – annual mortality 1-2%) • Prevalence – 25 000 (2000), 100 000 (2017), 180 000 (2030) CML treatment • Until 2000 – hydroxyurea, IFNα • Transplantation of bone marrow curative but high mortality • Gleevac – 10 year survival 80-90 % Gleevec (1993) Novartis • Imatinib mesylate • Active against CML colonies (Druker 1996) • 2 years later – clinical study: 31 patients, 98% response rate • Clinical study phase III: 16 countries, 177 centers, 1000 patients – study stopped, all patients on Gleevec • Survival 95%, survival 65% in blast crisis (8 years) • Molecular positivity of bcr-abl a problem - leukemic cells survive - danger of relapse? Current treatment of CML • Imatinib – in recent years even generics • Dasatinib • 350 More potent than imatinib • inhibition of Src pathway • five years survival similar to imatinib • Nilotinib • structural analogue of imatinib but binds better • Five-year survival better than imatinib • Bosutinib - Src/Abl inhibitor • for patients resistant to previous lines of therapy Cost of treatment • Imatinib – 30 000 USD/year – at the beginning • Today 132 000 USD/year • Generics – 8000 USD/year (2016) – but are they as efficient? CML diagnosis • 50% patients asymptomatic • Anemia, splenomegaly, fatigue, weight decrease • Cytogenetics for diagnosis • 100% of patients - bcr-abl, but also other aberrations (trisomy 8, …) • bone marrow biopsy Chronic lymphocytic leukemia - CLL Chronic lymphocytic leukemia - CLL • 30% of all leukemias • the most common type of leukemia in Western countries • clonal expansion of B cells - CD5 positive in peripheral blood, bone marrow, lymph nodes and spleen • more common in men (1.7:1) • Incidence 4.1/100 000 • Median age at diagnosis 67 Hallek 2019 CLL etiology • Genetics • Viruses (EBV, HIV) • Radiation • Chemicals • Smoking CLL genetic changes • primary changes in multipotent hematopoietic stem cells • Deletion 13q, deletion 11q, trisomy of chromosome 12 • Del(13q14) primary change - 55% of cases • Del(11q) - 25% of patients – deletion 11q23- gene ATM – decreased OS • Trisomy 12- 10-20% of patients • Del(17q) – 5-8% of patients – resistence to chemotherapy CLL diagnosis • Blood smear, immunophenotyping • More than 5000 B cells/1 μl of peripheral blood • Clonality based on flow cytometry CLL risk factors • deletion or mutation of TP53 • IGHV mutation (gene for heavy chain of immunoglobulin) • Serum β2 macroglobulin • Age over 65 CLL treatment • Chlorambucil – alkylator • Purine analogues - fludarabin, pentostatin, cladribin • Monoclonal antibodies – antiCD20 (rituximab) CLL Hematological malignancies Leukemia Lymphoma Multiple myeloma Lymphoma • malignant proliferation of lymphatic tissue – B, T cells • Solid tumor of blood cells • 1832 described by Dr. Hodgkin • most common hematological malignancy • 5.3 % of all tumors • Diffusing into other lymph nodes and tissues • Histology: • Hodgkin (more common in men) • Non-Hodgkin (B,T, NK cells) Lymphoma • Diffuse large B cell lymphoma (30 %) • follicular lymphoma (22 %) • MALT-lymphoma (8 %) • chronic B lymphocytic leukemia (7 %) • mantle cell lymphoma (6 %) Most common lymphoma: • Weight loss (10 % / 6 months) • Fever, night sweats All malignant lymphoma may present as B-symptoms: Hodgkin lymphoma • Painless enlargement of nodes (neck, axillary) • Fever, sweating, fatigue, weight loss • splenomegaly • Cough, emphysema • Infiltration of parenchymous organs • Etiology unknown – genetics, HIV, EBV • Common in adults between 20-30 and over 50 Hodgkin lymphoma •type I – lymphocyte –rich - majority of lymphocytes (few Reed-Sternberg cells, best prognosis) (5% of cases) •type II nodular-sclerosis (nodular deposits, cells – reticular, lymphocytes, histiocytes) in collagen fibres (70%) •type III mixed cellularity (20–25%) •type IV lymphocyte-depleted (ReedSternberg cells increased, worst prognosis) (1%) Reed-Sternberg buňky – abnormal lymphocytes, characteristic for lymphomas, multinucleated cells Hodgkin lymphoma Hodgkin lymphoma Non-Hodgkin lymphoma • Heterogenous group of tumors (cca 40 types) • Arising from lymph nodes – fast migration into surrounding tissues and metastases in children • At the time of diagnosis – 2/3 of patients have advanced stage of the disease • in children highly malignant tumors - very intense chemo treatment - successful in 80% of cases • In adults – less malignant Myelodysplastic syndromes - MDS Myelodysplastic syndromes - MDS • Heterogenous group of myeloid disorders characterized by cytopenia in peripheral blood and increased risk of progression into secondary AML • Incidence 3-4/100 000 (USA) • Prevalence increases with age • Diagnosis: bone marrow biopsy • Stratification: analysis of peripheral cytopenia, percentage of blasts in the bone marrow, cytogenetic analysis Cytogenetic classification of MDS • Mutations in TP53, RUNX1, ASXL1, JAK2 and RAS genes is connected to significantly shorter OS after allotransplantation of the bone marrow • TP53 mutations have a strong negative effect Survival of MDS patients depends on TP53 mutation Montelban-Bravo, 2018 Hematological malignancies Leukemia Lymphoma Multiple myeloma Multiple myeloma MM Hájek, 2012 Anderson, 2011 • second most common hematological malignancy • 10% of hematological malignancies • median age at diagnosis - 65 • Incidence 4/100 000 • more common in men • multistep pathogenesis Pathogenesis of MM - multistep process Jovanovic 2019 MGUS monoclonal gammopathy of unknown significance • accumulation of genetic changes in plasma cells leading to malignant transformation • In MGUS - bone marrow infiltrated by <10 % of malignant plasma cells • Asymptomatic – not found by routine tests • 15 % people with MGUS progress into MM • 1 % risk of progression to MM every year • Incidence 3 % of population over 50 (increases with age) MM • infiltration of bone marrow by malignant plasma cells • bone lesions • presence of monoclonal immunoglobulin (M-Ig) in serum and/or urine • Bone marrow niche supports proliferation and survival of malignant myeloma cells Plasma cell leukemia •loss of dependency of plasma cells on bone marrow microenvironment, migration into peripheral blood •> 20 % circulating plasma cells in periphery •Incidence 4/ 10 000 000 •transformation from MM - 21 months •Very bad prognosis - 2 - 3 months https://www.labmedica.com/hematology/articles/294776427/new-definition-for-plasma-cell-leukemia-proposed.html History of MM Male skull from the bronze age Capasso, 2005 Osteolytic lesions – typical feature of MM History of MM • 1844 - First documented case – Sarah Newbury (Dr. Solly) Kyle et Rajkumar, 2008 distraction of sternum broken bones distraction of femur •1845 – presence of protein in urine of a patient (Dr. Bence Jones – Bence Jones protein) •MM=Kahler disease – Prague MD Dr. Otto Kahler described MM History of MM Kyle et Rajkumar, 2008 Otto Kahler (1849-1893) healthy bone marrow MM bone marrow www.pathologyatlas.com MM symptoms 1) effect on bone marrow: •  erythrocytes → Anemia •  white blood cells → decrease of immune reactions •  thrombocytes k → bleeding • 2) Osteolytic lesions: • pain • fragile bones • fractures • calcium increase in serum • 3) presence of defective immunoglobulins • hyperviscosity • accumulation of these proteins in small veins • decrease of immunity - decreased number of regular immunoglobulins MM diagnosis quite difficult – pain, fatigue, repeated infections common for other diseases 1) number of myeloma cells in the bone marrow 2) presence of abnormal protein in blood or urine 3) typical changes on the bones Treatment of MM ….this is what we tried Hájek, 2012 Anderson, 2011 Treatment of MM …and this is what we're currently using • chemotherapy • transplantation of bone marrow • immunomodulatory drugs • proteasome inhibitors Hájek, 2012 Anderson, 2011 Prognosis of MM • untreated patients survive 14 months • standard therapy 3 - 4 years • Transplantation 6 – 7 years • New drugs increase five-year survival for about 80% of patients Hájek, 2012 Chemotherapy and transplantation • used even nowadays • treatment program junior vs senior (intensive versus less intensive) • Melphalan (alkylator) • Prednisone (Glukokortikoid – induces apoptosis of hematological cells) • Transplantation used since 1957 • Autologous – generally until 65 years of age of patient • Allogenous – rare, only in clinical trials Hájek, 2012 Anderson, 2011 Treatment possibilities for MM IMIDs (immunomodulatory drugs) Proteasome inhibitors •1953- created by Chemie Grünenthal •1957- distribution (without prescription) •Sedative •Relieves morning sickness •Heavy teratogen •Insufficient testing in animals •About 10 000 children effected – around 40 % survived •FDA - Dr. Francis Kelsey – did not allow usage of thalidomide in the United States Thalidomide – first IMID White House Archive Dr. Francis Kelsey (1914-2015) Thalidomide children… today Thalidomide – continuation • 1964 – Jason Sheskin – patient with leprosy and complications • 1993- Judah Folkman – angiogenesis important not only for solid tumors but also hematological • 1994 – refractory MM patient – thalidomide – clinical study 1/3 of patients responded • 2006 – FDA – treatment of MM approved • unpleasant side effects - neuropathy Sedlaříková, 2012 Treatment possibilities for MM IMIDs (immunomodulatory drugs) Proteasome inhibitors Proteasome inhibitors •Proteasome – a proteolytic complex for degradation of ubiquitinated proteins •MM cells produce large amount of proteins - inhibition of proteasome leads to accumulation of proteins in the cells and apoptosis •Bortezomib – first proteasome inhibitor approved for treatment of MM New drugs increase survival but do not cure ….not yet Thalidomid (Myrin) Bortezomib (Velcade) Lenalidomid (Revlimid) Carfilzomib ˃ Bortezomib Carfilzomib+Revlimid˃ Revlimid IV. Survival of patients with hematological malignancies https://www.lls.org/facts-and-statistics/facts-and-statistics-overview/facts-and- statistics and that is all …. Thank you for your attention