GENERAL EMBRYOLOGY 13.02.2023 Anna Mac Gillavry Danylevska FERTILIZATION Zygote – 18 h after fertilization 24 h 45 h 72 h Morula – 96 h Blastocyst CLEAVAGE AND IMPLANTATION cleavage Mitotic spindle within zygote Polar bodies MORULA BLASTOCYST Syncytiotrophoblast Primitive chorionic cavity Embryonic pole ENDOMETRIUM UTERINE GLANDS Trophoblast Embryoblast Extraembr. mesoderm Primitive yolk sac Chorion Operculum Amniotic cavity Trophoblastic lacunae Cytotrophoblast Endoderm Ektoderm Connecting stalk Amnion Germ disc Definitive yolk sac Extraembryonic coelom IMPLANTATION – DAY 7 Trophoblast Blastocyst cavity Hypoblast Uterine epithelium Syncytiotrophoblast Epiblast Blood capillary Uterine gland Uterine gland IMPLANTATION – DAY 8 1/2 Trophoblast Blastocyst cavity Hypoblast Cytotrophoblast Amniotic sac Syncytiotrophoblast Uterine gland Trophoblastic lacune IMPLANTATION – DAY 10 Primitive (primary) yolk sac Extraembryonic mesoderm Hypoblast Epiblast Cytotrophoblast Syncytiotrophoblast Trophoblastic Amniotic Primary chorionic lacune cavity villus IMPLANTATION – DAY 13 Extraembryonic Definitive (secondary) coelom yolk sac Secondary chorionic villus Hypoblast Epiblast Cytotrophoblast Syncytiotrophoblast Connecting stalk Trophoblastic lacune (extraembryonic mesoderm) (with capillary outlet) DORSAL SIDE OF GERM DISC – DAY 14 Wall of amniotic cavity Oropharyngeal membrane Connecting stalk Wall of yolk sac Hypoblast Epiblast Primitive streak GERM DISC - DAY 16 GERM DISC - DAY 17 DIFFERENTIATION OF MESODERM Somite Sclerotome Nephrotome Paired aorta dorsalis Aorta dorsalis Primitive gut Aorta dorsalis Dermatome Myotome Basis of arcus vertebrae Basis of corpus vertebrae paraxial MESODERM intermediate lateral Splanchnopleura Somatopleura