Medical Oral Microbiology Topic A - 10
Name: Date:
A11, p. 1
Oral microbiology II
Task 1: Proof of Streptococcus mutans in dental plaque
Direct proof of Streptococcus mutans in dental plaque can be performed with commercially available kits.
According to the assisstant´s instructions read the test for presence of Streptococcus mutans in dental plaque and
assess the risk of dental caries in this patient.
Task 2: Microflora of the oral cavity
According to the assisstant´s instructions prepare fixed
preparations of the swab of buccal tissue or upper part
of the tongue on the glass. Fix the preparation, stain
and observe with immersion objective magnifying
100× (total magnification 1000×).
Draw the results (consider shape and colour of present
bacteria and presence of other formations.
Task 3: Culture of common oral microflora
Study the Petri dishes with cultured microflora of the oral
cavity. Notice common microflora – note the alpha haemolytic
streptococci, look for the presence of oral neisseriae and
haemophili. Evaluate presence of some of pathogenic bacteria.
Draw and write your results.
Medical Oral Microbiology Topic A - 10
Name: Date:
A11, p. 2
Check-up questions:
1. Which bacteria are the parts of common oral microflora? Name at least five genera and five
species.
4. What are focal infections?
5. Why it is necessary in patients with valve defect to perform larger manipulations under
antibiotic cover?