Unit 6 Chromatography 1 What is the aim of chromatographic techniques? 2 What is the relation between the retention time and the speed of the analysis? 3 How do we measure efficiency? 4 What are the two main phases in chromatographic techniques? 5 What is the most important part of HPLC? 6 How do we put samples into the column? 7 In which phase do we use silica-based particles? 8 What is the commonest HPLC detection technique? Vocabulary warm-up stationary phase column retention time microsyringe device eluate analyte mobile phase theoretical plate chromatogram eluent sample injector flow volume 1__________ characteristic time it takes for a particular analyte to pass through the system 2__________ in many separation processes is a hypothetical zone or stage in which two phases, such as the liquid and vapour phases of a substance, establish an equilibrium with each other. 3__________ substance fixed in place for the chromatography procedure 4__________ phase that moves in a definite direction 5__________ substance to be separated during chromatography. It is also normally what is needed from the mixture 6__________ the visual output of the chromatograph 7__________ the mobile phase leaving the column 8__________ the solvent that carries the analyte 9__________ the matter analyzed in chromatography 10__________ a device used in conjunction with injecting samples 11__________ a glass tube with a diameter from 5 mm to 50 mm and a height of 5 cm to 1 m with a tap and some kind of a filter 12__________ an object or a piece of equipment that has been designed to do a particular job 13__________ a small pump with a plunger that fits tightly in a tube 14__________ amount of eluate passing through the column