Name: Study group:
This text was supported by project FRMU no. MUNI/FR/1552/2015 (Faculty of Medicine, Masaryk University).
Protocol
Estimation of heart rate
Procedures
1. Examination of pulse by palpation
Perform palpation in the arteria radialis, brachialis, carotis, femoralis and poplitea in three
persons. If you have palped the pulse, check the appropriate box in the table. Try all
mentioned arteries.
2. Resting heart rate according to a measured time interval
Resting heart rate is usually examined in a supine patient, but for this exercise a sitting
position will do. After a sufficient rest, count the pulse according the second hand on the
watch in intervals of 5, 10, 20, 30, and 60 seconds. Write down the results and convert
them to minute values (beats per minute).
3. Respiratory sinus arrhythmia
1. Attach the respiratory belt on the chest of the examined person and the finger pulse sensor
on the distal phalanx.
2. Start the program PULSE by double clicking on its icon.
3. Set the sensitivity of the amplifier for pulse registration (PULSE – 1st channel) and
respiratory movements (BREATHING – 3rd channel). Pulse frequency is calculated
automatically from the 1st channel and can be observed in the middle channel (PF –
2nd channel).
4. Record the following situations:
• resting breathing (1 minute)
• slowed breathing for 1 min (e.g. 4 seconds inspirium – 5 s expirium)
• faster breathing for 20 seconds.
5. Measure the pulse rate on top of inspirium and expirium from 5 breathing cycles at resting,
slowed and fast breathing (pulse rate value is given by position of mouse cursor). Calculate
average values of pulse rate on top of inspirium and expirium at resting, slowed and fast
breathing.
4. Heart rate in postural changes
1. Record the following situations
• at least 3 minutes of rest in standing position
• 2 minutes in supine position (lying on the bed)
• 2 minutes of rest in standing position
Changes between positions have to be quick
2. Count average heart rate from
• last 15 seconds of standing
• first 15 seconds of lying
• last 15 seconds of lying
• first 15 seconds of second time standing
Name: Study group:
This text was supported by project FRMU no. MUNI/FR/1552/2015 (Faculty of Medicine, Masaryk University).
• last 15 seconds of second time standing
3. Check the calculated values in the graph
5. Changes of heart rate after work load
1. After a few minutes of sitting at rest the heart rate is measured.
2. Then the examined subject performs 30 deep squats at a frequency of one squat per second.
Then we measure heart rate in sitting position each minute until initial values are reached.
Name: Study group:
This text was supported by project FRMU no. MUNI/FR/1552/2015 (Faculty of Medicine, Masaryk University).
Results
1. Examination of Pulse by Palpation
person a. radialis dx
a. radialis
sin.
a. carotis
dx.
a. carotis
sin.
a.
brachialis
a.
femoralis
a.
poplitea
1
2
3
Table 1: Place a tick where you have palped a pulse.
Describe the differences in the quality of pulse between measured persons
…………………………………………………………………………………………………
………………………………………………………………………………………………….
………………………………………………………………………………………………….
2. Resting heart rate according to the measured time interval
person 10 s bpm 20 s bpm 30 s bpm 60 s = bpm
1
2
3
Table 2: Write the number of pulses measured in time intervals (10, 20, 30 and 60 s) and
convert them into bpm (beats per minute).
Why do heart rate values counted at intervals shorter than 1 minute differ from one another?
………………………………………………………………………………………………..
………………………………………………………………………………………………..
Which time interval is long enough for estimation of heart rate?
………………………………………………………………………………………………..
Name: Study group:
This text was supported by project FRMU no. MUNI/FR/1552/2015 (Faculty of Medicine, Masaryk University).
3. Respiratory sinus arrhythmia
Resting breathing Faster breathing Slower breathing
Respiratory
cycle
inspirium expirium inspirium expirium inspirium expirium
1
2
3
4
5
average
Table 3: Heart rate measured in inspirium and expirium during different frequencies of
breathing.
Describe changes in heart rate depending on breathing.
………………………………………………………………………………………………….
………………………………………………………………………………………………..
How do the frequency and depth influence the heart rate difference between inspirium and
expirium?
…………………………………………………………………………………………………..
………………………………………………………………………………………………..
Name at least three possible causes of respiratory sinus arrhythmia formation.
1………………………………………………………………………………………………..
2………………………………………………………………………………………………..
3………………………………………………………………………………………………..
………………………………………………………………………………………………..
Name: Study group:
This text was supported by project FRMU no. MUNI/FR/1552/2015 (Faculty of Medicine, Masaryk University).
4. Heart rate in postural changesHeartrate[bpm]
200
Supine position
(3 min)
Laying position (2 min) Supine position (2 min)
180
160
140
120
100
80
60
40
End of orthostasis
Beginning of
clinostasis
End of clinostasis
Beginning of
orthostasis
End of orthostasis
Table 4: Heart rate measured at the beginning and end of postural change. Mark the heart rate
on the graph.
How do the postural changes influence heart rate?
…………………………………………………………………………………………………
…………………………………………………………………………………………………
Which mechanism of neural blood pressure regulation changes the heart rate? (one word)
…………………………………………………………………………………………………
Which part of the autonomic nervous system increases its activity during orthostatic reaction?
…………………………………………………………………………………………………
Name: Study group:
This text was supported by project FRMU no. MUNI/FR/1552/2015 (Faculty of Medicine, Masaryk University).
5. Changes of heart rate after work loadHeartrate[bpm]
200
180
160
140
120
100
80
60
40
Resting
values
0 minute
after
squatting
1 minute
after
squatting
2 minutes
after
squatting
3 minutes
after
squatting
4 minutes
after
squatting
5 minutes
after
squatting
Graph 3: Heart rate changes at rest and after squatting. Mark the heart rate on the graph.
Describe changes of heart rate measured before and after physical activity.
……………………………………………………………………………………………….
……………………………………………………………………………………………….
…………………………………………………………………………………………………..
Conclusion
Summarize heart rate changes observed under various physiological conditions.
…………………………………………………………………………………………………..
…………………………………………………………………………………………………..
…………………………………………………………………………………………………..