Extraction of chlorophyl


This method is convenient to make extract for the photospectrometre.


  * Polish a couple of leaves in a mortar. Spinach is very suitable, but other leaves are possible
as well.

  * Pipette  4 parts (for example 4 ml) aceton .
  * Let it for 5 minutes in teh  dark (cover up because of the vapour)
  * Pour the liquid into a test-tube.


  * Add 4 parts (ml) of water and 3 parts of petroleumether (be carefull benzine!!)
  * Put a rubber stopper on the tube and shake powerful.
  * The watersoluble  matters are mainly in the aceton-watermixture. The fatsoluble matters in the
layer of petroleumether.
  * Pipette the layer with chlorophyl.
  * Evaporate the petroleumether in de sourcaseif the chlorophyl is not concentrated enough
(explosion danger!! Ask the assistent)

papierchromatografie of chlorofyl

Material:

  * Some leaves
  * Mortar met pestle
  * Clean silversand
  * aceton?
  * petroleumether 40-60°C?
  * liquid (petroleumether 40-60°C - aceton 9:1)
  * chromatograficpaper
  * capillair
  * pencil



  * Do everything in a fire free room .
  * Put the leave partikels in the mortar.


  * Add a little silversand and polish them.
  * Add a little aceton.Zie ook techniekkaart 2.8
  * Polish again until a green liquid arises. (extract).
  * Take  a slip of chromatografiepaper.
  * Just touch the edges.
  * Draw a line down below at 2 cm of the edge with a pencil (= startline)
  * Put the filtreerpapier on a clean background and draw with the capillair a line of chlorophyll
on the start line


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  * Dryg the spot with a föhn. Repeat this 10 to 20 times.
  * Try to keep the spot as small as possible.
  * Pour the liquid into a cilinder glas. Not more than 1.5 cm high.
  * Hang the paper on the cork with the bottom in the liquid
  * De chlorophylvspot must be above the liquid and the edges of the paper must not touch the side
of the glass..
  * When the liguid is almost above (after 25 minutes), remove the chrmomatogramme
  * Mark with the pencil the front and the place of the coloured spots.

The distance (rF-value) is calculated for every spot by dividing  the distance (A) by the distance
between start and front(B).De rF- value is by a certain T and a certain liquid characteristic for a
matter. The rF- values of the chlorophyll can be looked up.

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