Syllabus Pathobiochemistry 2017/2018 45-224
1. Introduction, the importance of studying pathobiochemistry. The scope and requirements for successful completion of the course
exam, recommended literature. Understanding the regulation of metabolism. Hormones. Biochemical communication.
2. The nucleic acid metabolism disorders of purine and pyrimidine. Hyperuricemia, orotacidurie, therapy.
3. Metabolism disorders, types and causes. Hereditary metabolic diseases. (16.2. MB)
4. Amino acid metabolism and its disorders. Types of diseases and therapy.
5. Disorders of lipid metabolism. Cholesterol, lipoproteins. Lipidosy, dyslipoproteinaemia
6. Pathobiochemistry of carbohydrates, glucose metabolism and its disorders. Glycemic control disorders. Pathobiochemistry of
diabetes mellitus, types of DM. Disorders of glycogen metabolism, glykogenosis. (16.3. MB)
7. Analysis of urea and the urinary sediment. Immunochemical methods. (27.3.JJ)
8. Mechanization and automation in clinical biochemistry. Analyzers, their distribution from different perspectives. Diagnostic kits. The
organization of work in clinical-biochemical laboratory, laboratory and hospital information systems. (27.3. JJ)
9. Pathobiochemistry of arteriosclerosis. Ischemic heart failure - cardiac markers. (27.3 JJ)
10. Relation between Pathobiochemistry and Clinical Biochemistry. Clinical and biochemical analysis and its specific features.
Terminology of Clinical Biochemistry. The analyzed material. Material removal. (27.3 JJ)
11. Blood, blood plasma proteins. Blood clotting, coagulopathy. Dysproteinaemias. Porphyrins. Biosynthesis, metabolism disorders.
Porphyria, hemoglobinopathies (practical lecture 3.4. Kučerová, 45-321).
12. Enzymes, regulation of metabolism. Causes increased activity of cellular enzymes in the plasma. Clinically significant enzymes.
Xenobiotics and their effects on the body. Detoxification mechanism. Biological oxidation. The effects of free radicals on the
organism. Lipoperoxidation antioxidants. (13.4.)
13. Tumor, tumor markers. Basic characteristics of tumor cells. Strategy laboratory tests. Requirements ideal tumor marker. Used tumor
markers. (20.4.+ credit test)
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Syllabus of practical exercises:
1. Practice: Analysis of tumor suppressor by
immunodetection on membrane. (23.2. MB)
2. Practice: Basic biochemistry. Biochemical analyzer
Dimension. (23.3. CL)
3. Practice: Immunochemical methods. Immulite
Immunoassay Analysator. (20.3. CL)
4. Practice: Hematologic methods. (3.4. –lecture, 45-
321, Mgr. Kučerová-have to be confirmed)
5. Final test. (20.4. MB) and lecture (45-224)
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