Bi 6120c   Plant Cell, Tissue and Organ Culture

   Demands for Laboratory Protocols

   

   Obligatory parts of the protocol:

   1.      Name

   2.      Date

   3.      The topic of an experiment (= the aim of an experiment)

   4.      The principle of a method used

   5.      Material and equipment, components of media (mineral  and organic nutrition, agar,
   sucrose and growth regulator concentration)

   6.      A brief procedure (disinfections, method of explant isolation and maintenance)

   7.      The results (will be recorded gradually during the culture period = contamination
   rating, changes of explant appearance etc.)

   8.      Conclusion