Syllabus: Plant Cell, Tissue, and Organ Culture – Lecture

                           School Year 2006-2007, Spring Term 2007

   15.3.07       Introduction: Definitions, Culture Vessels and Different Types of Culture,
   History and Procedure Develoment, Literature, Conditions of Axenic Culture, Laboratory Design,
   Nutrient Media Preparation and Sterilization.

   29.3.07      Phytohormones and Growth Regulators: Auxines, Gibberellines, Cytokinines,       
   Abscisic Acid, Ethylene, Further Regulatory Substances (Brassinosteroids,        Jasmonic
   Acid, Polyamines, Oligopeptides)

   5.4.07         Micropropagation: Types of Regeneration: Restitution, Reproduction (Propagation
   by Axillary Bud Proliferation), de novo Regeneration (Adventitious Shoot Proliferation),
   Primary Culture Initiation, Meristem Cultures, Virus Elimination, Herb and Wood Meristem
   Thermotherapy, Mas Propagation.

   12.4.07       Callus Culture: Callus Initiation, Callus Culture Utilization,

   19.4.07       Secondary Metabolites and Biotechnology: Elicitation, Suspension Culture,
   Bioreactors, Immobilization, and Bioconversion. Fungi Culture: Suspension Culture of
   Wood-Destroying Fungi.

   26.4.07       A. Test-tube Pollination/Fertilization: Barriers of Incompatibility and
   Possibilities of their Overcoming in vitro, Remote Hybridization, Pollen Quality and its
   Evaluation. B. Zygotic Embryo Culture: Developmental Stages of Zygotic Embryo, Isolation and
   Culture of Young and Mature Zygotic Embryos in vitro, Method Utilization (Break of Dormancy,
   Shortening of Breeding Cycle, Embryo Rescue Technique, Source of Meristematic Material for
   Propagation). C. Somatic Embryogenesis: Direct and Indirect Somatic Embryogenesis, Factors of
   SE, and Origin of SE, Artificial Seeds, Conversion of Somatic Embryos.

   3.5.07         A. Induction of Haploid Plants: a) Direct and Indirect Androgenesis: Anther and
   Microspore Cultures, Pollen Embryogenesis, Dihaploidization, Influence of Ontogenetic Stage of
   Pollen Development, Influence of Iron Concentration on  Nicotiana  Androgenesis, b)
   Gynogenesis. B. Cryoprezervation: Cryoprotective Substances, Methods of Slow and Quick
   Freezing,Vitrification, Encapsulation /Dehydratation, Thawing, Tests of Plant Material
   Viability, Utilization – Gene banks.

   10.5.07       Plant Genome Transformation: Genetic Modified Organisms (GMOs), Legislative,
   Structure of Chimeric Genes, Detection of Transgenosis - Marker and Selectable Genes, Methods
   of Transformation – Disc method, Vacuum Infiltration, Biolostic Method, Agrobiolistic Method.

   17.5.07       Excursion at the Plant Tissue Culture Lab of Mendel Agriculture and Forestry
   University and Credit.

   

   

   

   

   

   Recomended Literature

   

   1.    FOSSARD, de R.A. (1976): Tissue Culture for Propagators.- Univ. New England Printery.

   2.    GAMBORG,  O. L. et PHILLIPS, G. C. (1995): Plant Cell, Tissue and Organ Culture.
   Fundamental Methods, Springer Berlin. Heidelberg.

   3.    PIERIK, R.L.M. (1987): In vitro Culture of Higher Plants. - Martinus Nijhoff Publishers,
   Dordrecht, Boston, Lancaster.

   4.     RAGHAVAN, V. (1976): Experimental Embryogenesis in Vascular Plants.- Academic Press
   London, New York, San Francisco.

   5.     REINERT, J. et BAJAJ, Y.P.S./eds./, Applied and Fundamental Aspects of Plant Cell,
   Tissue and Organ Culture.- Springer Verlag, Berlin, Heidelberg, New York, 1977.

   6.     REINERT, J. et YEOMANN, M.M.(1982): Plant Cell and Tissue Culture. A Laboratory
   Manual.- Springer Verlag Berlin etc.

   7.     SASSON, A. (1988): Biotechnologies and Development.- UNESCO, Paris.

   8.     THORPE, T.A. (1995): In vitro Embryogenesis in Plants.- Kluwer Academic Publishers,
   Dordrecht, Boston, London.