+--------------------------------------------------------------------------------------------+ |Pts|Nucleic acids metabolism, proteosynthesis |Note| |---+-----------------------------------------------------------------------------------+----| | | | | |---+-----------------------------------------------------------------------------------+----| |20 |Describe the course of translation (chemical reactions, scheme of individual steps)| | |---+-----------------------------------------------------------------------------------+----| | | | | |---+-----------------------------------------------------------------------------------+----| | | | | |---+-----------------------------------------------------------------------------------+----| | | | | |---+-----------------------------------------------------------------------------------+----| |15 |Describe the formation of aminoacyl-tRNA | | |---+-----------------------------------------------------------------------------------+----| | |Describe the reverse transcripton – scheme, importance | | |---+-----------------------------------------------------------------------------------+----| | |Describe the basic principle of the gene expression regulation (Monod) | | |---+-----------------------------------------------------------------------------------+----| | |Describe the reaction sequence of eukaryotic transcription up to functional | | | |product! | | |---+-----------------------------------------------------------------------------------+----| | |Describe PCR – steps, reactions! | | |---+-----------------------------------------------------------------------------------+----| | |Replication – chemical reactions, substrates | | |---+-----------------------------------------------------------------------------------+----| | | | | |---+-----------------------------------------------------------------------------------+----| | | | | |---+-----------------------------------------------------------------------------------+----| |10 |Describe schematically the replication process – individual steps! | | |---+-----------------------------------------------------------------------------------+----| | |Describe the synthesis of pyrimidine nucleotides! | | |---+-----------------------------------------------------------------------------------+----| | |Describe at least schematically the synthesis of purine nucleotides, substrates, | | | |coenzymes! | | |---+-----------------------------------------------------------------------------------+----| | |What means maturation of proteins, typical reactions, examples, importance? | | |---+-----------------------------------------------------------------------------------+----| | |Describe the catabolism of purines | | |---+-----------------------------------------------------------------------------------+----| | | | | |---+-----------------------------------------------------------------------------------+----| | | | | |---+-----------------------------------------------------------------------------------+----| |5 |What are the restriction endonucleases, characteristic reactions, importance, use? | | |---+-----------------------------------------------------------------------------------+----| | |What means the genetic code, general description? | | |---+-----------------------------------------------------------------------------------+----| | | | | |---+-----------------------------------------------------------------------------------+----| | | | | +--------------------------------------------------------------------------------------------+