Fundamentals of modern UV-visible spectroscopy Presentation Materials The Electromagnetic Spectrum Electronic Transitions in Formaldehyde Electronic Transitions and Spectra of Atoms Electronic Transitions and UV-visible Spectra in Molecules Derivative Spectra of a Gaussian Absorbance Band Resolution Enhancement Transmission and Color Absorbance and Complementary Colors Transmittance and Concentration The Bouguer-Lambert Law Transmittance and Path Length Beer’s Law The Beer-Bouguer-Lambert Law Two-Component Mixture Two-Component Mixture Influence of 10% Random Error Absorption Spectra of Hemoglobin Derivatives Intensity Spectrum of the Deuterium Arc Lamp Intensity Spectrum of the Tungsten-Halogen Lamp Intensity Spectrum of the Xenon Lamp Dispersion Devices Photomultiplier Tube Detector The Photodiode Detector Schematic Diagram of a Photodiode Array Conventional Spectrophotometer Diode-Array Spectrophotometer Diode-Array Spectrophotometer Conventional Spectrophotometer Diode-Array Spectrophotometer Conventional Spectrophotometer Definition of Resolution Instrumental Spectral Bandwidth Natural Spectral Bandwidth Effect of SBW on Band Shape Effect of Digital Sampling Wavelength Resettability Effect of Stray Light Theoretical Absorbance Error Effect of Drift Transmission Characteristics of Cell Materials Cell Types I Cell Types II Effect of Refractive Index Non-planar Sample Geometry Effect of Integration Time Effect of Wavelength Averaging Increasing Dynamic Range Scattering Scatter Spectra Isoabsorbance Corrections Background Modeling Internal Referencing Three-Point Correction Discrimination of Broad Bands Scatter Correction by Derivative Spectroscopy Effect of Fluorescence Acceptance Angles and Magnitude of Fluorescence Error Inadequate Calibration Calibration Data Sets Wavelength(s) for Best Linearity Wavelength(s) for Best Accuracy Precision of an Analysis Wavelength(s) for Best Sensitivity Wavelength(s) for Best Selectivity Ideal Absorbance and Wavelength Standards Ideal Stray Light Filter Holmium Perchlorate Solution Potassium Dichromate Solution Stray Light Standard Solutions Toluene in Hexane (0.02% v/v) Confirmation Analysis Spectral Similarity Precision and Accuracy Hydrolysis of Sultone