2010 © Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. EasyCyte is a trademark of Guava Technologies, Inc. Pippin Prep is a trademark of Sage Science, Inc. Bioruptor is a trademark of Diagenode SA. BioAnalyzer is a trademark of Aglient Technologies, Inc. IonTorrent The Chip is the Machine scalability simplicity speed discover Ion Torrent The Ion Torrent Personal Genome Machine (PGM™) is simpler, more cost effective, and more scalable than any other sequencing technology. The PGM™ sequencer is a bench top system utilizing ground breaking and disruptive semiconductor technology that enables rapid and scalable sequencing experiments. Ion Torrent technology uses a massively parallel array of proprietary semiconductor sensors to perform direct real time measurement of the hydrogen ions produced during DNA replication. A high-density array of wells on the Ion semiconductor chips provide millions of individual reactors while integrated fluidics allow reagents to flow over the sensor array. This unique combination of fluidics, micromachining, and semiconductor technology enable the direct translation of genetic information (DNA) to digital information (DNA sequence) rapidly generating large quantities of high quality data. The Personal Genome Machine along with Ion Torrent semiconductor chips, Ion Torrent reagent kits, and the Torrent Server / Torrent Suite software allow Ion Torrent to deliver a cutting edge sequencing solution. scalability massively scalable semiconductor technology simplicity natural biochemistry, no optical components speed runs in about two hours, not days or weeks discover scalability Scales like a semiconductor • Leverages $1 trillion semiconductor industry investment • Scales by density: 40 cumulative years of Moore’s Law • Scales by volume: 400 standard semiconductor foundries from which to choose discover simplicity Simple chemistry • Simplest sequencing chemistry: hydrogen ion detection (pH meter) • Natural nucleotides: no enzymatic cascade, no fluorescence or no chemiluminescence • Low cost standard reagents discover speed Direct, real-time detection technology • Makes sequencing accessible to new applications and markets • Short run time supports fast research cycle and faster time to publication • The Chip is the Machine™: no optics, no light, no scanning sequencing and analysis pipeline The Ion Torrent sequencing workflow consists of a number of standard elements — creation of a shotgun fragmented DNA or amplicon library, a simple PCR process creating amplified templates on proprietary Ion Sphere™ particles, followed by a sequencing run on the PGM™ platform. With the completion of the PGM™ run, data automatically transfers to the dedicated Torrent Server that runs Torrent Suite software. Here raw ion signals are converted to base calls and stored in industry standard SFF or FASTQ files. These data can be processed by a variety of commercially available software packages for variant detection or denovo genome assembly. Ion Torrent Part Numbers Personal Genome Machine Ion PGM™ Sequencer 4462917 Service Contract ZGEXSCIONPGMSYS ZG11SCIONPGMSYS Torrent Server 4462918 Semiconductor Sequencing Chip Ion 314 Chip Kit ( 8 Chips) 4462923 Reagent Kits Ion Fragment Library Kit 4462907 Ion Template 314 Kit 4462908 Ion Sequencing 314 Kit 4462909 Ion Control Materials Kit 4462913 Contact In North America, contact Ion Torrent at: 1-87-SEQUENCE (1-877-378-3623) Outside of North America, contact Ion Torrent at: +1-203-458-8552 www.iontorrent.com Scale 1.3 million wells Data Output Starting at 10Mb of high-quality sequence* Data Format SFF/FASTQ file output Reagents Complete set of 314 reagents, including library, template, and sequencing kits Applications Sequencing or resequencing microbial genomes Targeted (amplicon) resequencing Ion 314 Sequencing Chip For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. 13 January 2011 CO17137 * Throughput calculated with Escherichia coli DH10B genome, using Ion Sequencing 314 chemistry and a 100 bp read length PGM™ sequencer configuration.