Introduction
This tutorial is intended to help new users with placing an order for gene synthesis services, including
the steps needed for gene optimization prior to placing an order. Video tutorials are also available at
www.lifetechnologies.com/genesynthesis
Contents
Introduction ................................................................................................................................................1 
1. Setting Up a Project.................................................................................................................................3 
1.1. How to Start an Online Request.......................................................................................................3 
1.2 Project Manager Features to Organize Projects..............................................................................4 
1.2. How to Use the Project Configurator for Project Setup, e.g., Gene Synthesis ...............................5 
2. Gene Synthesis and Online Optimization ................................................................................................8 
2.1. Gene Synthesis Project Setup..........................................................................................................8 
2.2. Entering a Sequence That Does Not Need Any Editing or Optimization.........................................9 
2.3. Editing and Optimizing a Sequence ...............................................................................................10 
2.3.1. Defining the ORF in Preparation for Optimization .................................................................10 
2.3.2. Selecting 5’ and 3’ Cloning Sites ............................................................................................11 
2.3.3. Add, Delete, or Exchange Sequence Parts.............................................................................12 
2.3.4. Optimizing a Sequence ...........................................................................................................13 
2.4. Sequence Summary .......................................................................................................................14 
2.5. Information Complete....................................................................................................................16 
2.6. MultiSequence Batch Upload.........................................................................................................17 
3. Subcloning Into Vector of Your Choice..................................................................................................22 
3.1. Subcloning Project Setup...............................................................................................................22 
3.2. Enter Subcloning Requirements Using a Life Technologies Vector .............................................23 
3.3. Enter Subcloning Requirements Using Your Own Vector .............................................................24 
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3.4. Vector Summary.............................................................................................................................26 
4. Strings™ DNA Fragments .....................................................................................................................27 
4.1. How to Set Up a DNA Fragment Order ..........................................................................................27 
4.2. MultiSequence Batch Upload for Strings™ DNA Fragments........................................................30 
5. Setup of Mutagenesis: Single Gene Variants........................................................................................32 
5.1. Which Sequence Qualifies as a Single Gene Variant? ...................................................................32 
5.2. Single Gene Variant Project Setup.................................................................................................33 
5.3. Entering Variant Sequence Information ........................................................................................35 
5.4. Additional Information ...................................................................................................................36 
5.5. Information Complete....................................................................................................................37 
6. Setup of Mutagenesis Bundles (Gene Variants)....................................................................................38 
5.1. Which Sequences Qualify as Mutagenesis Bundles? ....................................................................38 
6.2. Mutagenesis Bundle Project Setup ...............................................................................................39 
6.3. Entering Several Gene Variant Sequences....................................................................................40 
6.3.1. Setting Up a Mutagenesis Bundle With the Multifasta Option...............................................41 
6.3.2. Setting Up a Mutagenesis Bundle With the Parent Service Option.......................................44 
7. Adding a Project to Cart and Placing Your Order .................................................................................48 
7.1. Proceed to Project Summary.........................................................................................................48 
7.2. Project Summary ...........................................................................................................................49 
7.3. Cart View ........................................................................................................................................51 
7.4. Shipment & Payment Information .................................................................................................52 
7.5. Review Order..................................................................................................................................54 
7.6. Order Confirmation ........................................................................................................................55 
8. Contacts at GeneArt ..............................................................................................................................56 
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1. Setting Up a Project
1.1. How to Start an Online Request
 After login, your starting screen will be the “Project Manager” (1) on the “My Projects”
tab (2). The Project Manager will allow you to create new projects and organize existing
ones.
 For any kind of GeneArt® online service, first click the button “+ New Project” (3), name
your project (4), and then click “Select a folder”.
 On the subsequent dropdown menu you can either choose “Create new folder” [5] or
select from your existing ones if you are a returning user (6).
 If you are creating a new folder, the next step is to enter a “Folder name” [7).
 Then click the “Start Project” button (8). You will be directed to the “Project
Configurator” (9), where you can design the service parts of your new project and enter
your requirements.
 You can go back to the “Project Manager” step by clicking on the “My Projects” tab at any
time (2).
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1.2 Project Manager Features to Organize Projects
 Click this button (10) to edit or view your project in the Project Configurator.
 Hover over (11) to display a menu where you can rename, move, or delete your project.
You can also add attachments.
 Click (12) to immediately move to the project summary, e.g., for ordering.
 Some projects will require review by our specialists, and different project versions will be
created during this review. To see a chronological history of the status points in the
project, click "Show project history"(13).
 Click Project Name, Status, Date, Project ID, or Folder in the header bar (14) to sort your
projects by that group. You can toggle the sort order by clicking the same group header
again.
 The current active sort group is indicated by darker highlighting (15).
 To search, type a project ID, project name, folder name, or gene name (case-insensitive,
no asterisks) here (16).
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1.2. How to Use the Project Configurator for Project Setup, e.g., Gene Synthesis
 The Project Configurator displays all available services as drag & drop icons (19),
organized in tabs of service groups, e.g., Gene Synthesis (17). To receive more
information about each service, and how to use it, click the question mark (18).
 To start a gene synthesis project, drag the Gene Synthesis icon (19) onto the drop area
(20).
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 To order additional services for your project, such as subcloning (21), drag and drop the
icon for the service you need onto the Gene Synthesis icon that you created in the
Process area (22).
 If you require larger amounts of your clone DNA, you can add the Plasmid Preparation
service (23). If you want us to only produce your plasmid DNA, start a Process with just
the Plasmid Preparation icon.
 Add any additional services the same way. Allowed services will be highlighted by a green
frame when mousing over an icon in the upper panel.
 If you require site-directed mutagenesis or gene variations, use the Mutagenesis icon
(24). For larger numbers of constructs, use the Mutagenesis Bundle icon (25).
 If you need ready-to-clone gene fragments (a fast and affordable alternative to full gene
synthesis), use the Strings™ DNA Fragments icon (26).
 For precise targeting and editing of a genome, you can request clones for custom DNAbinding
proteins with various effectors, using the Precision TAL icon on the Precision
TAL tab (27).
 We also offer the option to enter several sequences at once using the MultiSequence
Batch Upload icon (28).
 For assisted setup with the help of our specialists or for projects that need a higher level
of customization, please use the Assisted Setup icon (29), which is a file upload function
(supported file formats: doc, docx, xls, xlsx, ppt, pptx, pdf, zip, rar, gb, fas, jpg, jpeg, bmp,
png, gif, tif, tiff, and txt). As much as possible, we try to process projects via the
automated ordering on the online portal. For Assisted Setup, additional charges may
apply.
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 Each icon comes with a menu of functions (30) to help you enter and edit your
information (“Edit”) or organize parts of your projects. “Copy Item” allows you to copy a
service that already has been created and defined (e.g., subcloning) and apply it to
another item (e.g., a gene) by “pasting” it into the target menu.
 All icons are accompanied by a status indicator in the top left corner, for a status
overview.
A green check mark indicates that a setup is complete, i.e., has all information
required to technically process the request. Please ensure that you have added any
individual information and requirements nevertheless. You can then “Proceed To
Summary”.
A red exclamation mark (31) lets you know that more information about the
requested service is needed. Please click the icon to open the service form and
complete it.
The red status might also appear if you have copied a service. To address this, you
simply open the form, then save and close it again without adding any information.
In addition, if some service information is changed the dependent services might
need to be validated again. Please open the form, adjust relevant information if
necessary, and then save and close it. If no changes are required, just save and close
it.
A yellow status indicator lets you know that, in its current form, your project is
not suitable for immediate ordering.
Subcloning: Please review cloning information for errors.
Mutagenesis: Does not fulfill variant criteria; you may need the Gene Synthesis
service.
In either case, you can ”Proceed To Summary” to submit for specialist review.
 Our “Gene Synthesis and Online Optimization” tutorial in chapter 2 covers the details of
completing the Gene Synthesis service request form, including how to complete online
sequence optimization for maximum expression in your host.
 Other tutorial chapters cover ordering of our Subcloning service (chapter 3) and
Mutagenesis with Single and Multiple Gene Variants (chapters 4 and 5).
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2. Gene Synthesis and Online Optimization
2.1. Gene Synthesis Project Setup
 To begin, drag and drop the Gene Synthesis icon (1) onto the drop area (2).
 The red exclamation mark (3) indicates that more information is needed.
 Click the Gene Synthesis icon to open the form (4).
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2.2. Entering a Sequence That Does Not Need Any Editing or Optimization
 The Gene Synthesis form has three main tabs:
the Enter Sequence tab, the Edit Sequence tab, and the Optimize Sequence tab.
 The Enter Sequence tab is for entering the sequence and providing all basic information.
 Enter your sequence name (5), choose whether to enter your sequence as DNA or protein
(6), and paste your sequence into the tool (7).
 Select the biosafety level for your project (8).
 The lower part of the form (9) allows you to add general information about your sequence
and select additional service options.
 Please note that speed upgrades can be chosen later in the process on the project
summary page, before you add your project to the cart.
 The Enter Sequence tab is the only tab you need to complete if your sequence is already
defined and ready to order.
 At any time you can review your sequence in the Sequence Summary tab (10). Here you
can download the sequence in different formats, as well as a printable PDF version of the
sequence summary; see chapter 2.4.
 If no further edits or optimization are required, you can proceed to order by clicking the
“Save & close” button (11).
 For checkout and ordering, please read the “Adding a Project to Cart and Placing Your
Order” tutorial in chapter 7 to see how that process works.
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2.3. Editing and Optimizing a Sequence
2.3.1. Defining the ORF in Preparation for Optimization
 The Edit Sequence tab is required to edit and prepare your sequence for optimization.
 Start here to choose (12) or define (13) the ORF you need for optimization. If your ORF is
not displayed, click the “Show more” link (14).
 You can also add start and stop codons to your ORF (15).
 All your annotations (ORF, cloning sites, etc.) are shown in the Feature Map (16).
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2.3.2. Selecting 5’ and 3’ Cloning Sites
 To annotate cloning sites (17), just select from the detected ones (18) or select from a list (19) or
define new ones yourself (20).
 Selected sites will remain unchanged during optimization, and the motif will not get generated in the
optimized sequence.
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2.3.3. Add, Delete, or Exchange Sequence Parts
 On the Add/Delete/Exchange tab (21) you have separate options to add, delete, or
exchange sequences. The exchange function works at the codon or amino acid level and
therefore requires an ORF to be defined.
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2.3.4. Optimizing a Sequence
 For optimization with our proprietary GeneOptimizer® algorithm, go to the Optimize
Sequence tab (22).
 Define which expression host the sequence should be optimized for (23). Please note that
depending on the expression organism, a feature will be available for adding the
appropriate Kozak sequence (24).
 If you would like to receive a specialist optimization or review instead of optimizing
online, you can select that option as well (25).
 Please note that there are optional functions that allow you to protect sites and motifs or
exclude motifs from getting created during optimization (26).
 Ensure all sequence editing and specification is complete. Then click the “Optimize”
button (27) to begin online optimization.
 The duration of optimization will depend on the sequence length and complexity.
Typically, a standard sequence of approximately 1 kb will take about 30 seconds to be
processed.
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2.4. Sequence Summary
 The Sequence Summary tab (28) displays an overview of the actual sequence and all
edits made (29). The sequence as well as the summary can be downloaded (30).
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 Graphical data display the quality of your sequence before and after optimization.
 The top two histograms (32) and (33) compare the quality distribution of codons in the
sequence before and after optimization, in terms of the percentage of codons that fall
into different quality classes. In the optimized sequence, the majority of codons used
have quality values greater than 80.
 The second set of plots shows codon quality at each position in both non-optimized (34)
and optimized sequences (35). Again in this view, it’s clear that the number of codons
with quality scores below 80 decreases dramatically after optimization.
 The final set of plots (36) and (37) shows GC content in the non-optimized and the
optimized sequence. The optimization algorithm adjusts GC content for increased mRNA
stability, when appropriate for the expression host.
 Please note, you can return to the previous tabs to make modifications at any time.
Remember to click the “Optimize” button again each time when you want an optimized
sequence.
 Save & close the form (31).
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2.5. Information Complete
 The green status indicator should now be shown (38). If you are ready to place your
order, click “Proceed To Summary” (39).
 The next step is to add the project to your cart and proceed to checkout. Please read the
“Adding a Project to Cart and Placing Your Order” tutorial in chapter 7 to see how that
process works.
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2.6. MultiSequence Batch Upload
 If you intend to order multiple sequences at the same time, our MultiSequence Batch
Upload (40) can make your ordering process faster.
 To get started, drag and drop the MultiSequence Batch Upload icon (40) onto the drop
area (41).
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 The first step is to choose whether to enter DNA or protein sequences (42). In either
case, you’ll need FASTA- or Multi-FASTA–formatted sequences ready for pasting into the
sequence field, as in the example shown [43]. In this example we’ve entered three DNA
sequences.
 Next, read the biosafety confirmation and select the biosafety requirements for your
project (44].
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 If you need additional services that will be applied to all of your sequences, you can
choose them here (45).
 When you’re finished, click “Save” (46).
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 For each sequence, a separate gene synthesis process is set up [(47), (48), (49)]. The red
exclamation marks (50) indicate that more information is needed. You’ll need to complete
each Gene Synthesis process form.
 Click the first Gene Synthesis icon (51) to open the form.
 The sequence (52) and an arbitrary name (53) are already filled in. You’ll need to complete
the rest of the form. To learn more about completing the Gene Synthesis form, please
read the “Gene Synthesis and Online Optimization” tutorial in chapter 2.
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 When you have completed the Gene Synthesis form, the green status indicator should be
shown (54).
 Continue this process to complete the form for each gene synthesis construct in your
project.
 Click the “Proceed To Summary” button (55). Then you can add the project to your cart
and proceed to checkout. Please read the “Adding a Project to Cart and Placing Your
Order” tutorial in chapter 7 to see how that process works.
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3. Subcloning Into Vector of Your Choice
3.1. Subcloning Project Setup
 After setting up the gene synthesis project, drag and drop the Subcloning icon (1) onto the
Gene Synthesis icon in the Process area (2). Please note: For subcloning, 5’ and 3’ cloning
sites have to be specified in the Gene Synthesis form.
 Next, open the Subcloning form by clicking the Subcloning icon (3).
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3.2. Enter Subcloning Requirements Using a Life Technologies Vector
 Enter a name for the final plasmid (4).
 Then choose from the list of Life Technologies vectors (5).
 Cloning sites will be predefined, based on the cloning site information provided in the
Gene Synthesis form (6).
 Next, the portal will automatically clone your predefined gene into the selected vector (7).
 If required, please select the alternative cloning strategy (8).
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3.3. Enter Subcloning Requirements Using Your Own Vector
 If you require subcloning into a custom vector, choose the option “Custom vector” (9)
from the list.
 Select the copy number of the destination vector (10) and the resistance marker (11).
 Indicate whether the vector is stocked at GeneArt® or is a new vector that will need to be
sent to us (“I will provide”) (12).
 Provide us the vector name (13) and paste your vector sequence into the vector sequence
field (14). Alternatively, upload your sequence (15).
 Then click “Start cloning” (16).
 As of October 2012, any vector ordered via the above-mentioned route will be added to
your “My portal vectors” list (17) so that it is readily available for your next order.
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 The portal will automatically clone your predefined gene into the selected vector (18).
 If required, please select the alternative cloning strategy (19).
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3.4. Vector Summary
 The Vector Summary tab includes your specifications (20), the final map (21), and the
final vector information in GenBank® database format together with a download function
(22).
 Click “Save & Close” (23).
 Proceed to the summary to add your gene synthesis and subcloning to the cart. Please
read the “Adding a Project to Cart and Placing Your Order” tutorial in chapter 7 to see
how that process works.
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4. Strings™ DNA Fragments
4.1. How to Set Up a DNA Fragment Order
 To set up a DNA Fragment order, drag the Strings™ DNA Fragments icon (1) onto the
drop area (2).
 The red exclamation mark (3) indicates that more information is needed.
 Click the Strings™ DNA Fragments icon (4) to open the form.
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 The Strings™ DNA Fragments form has three main tabs:
the Enter Sequence tab (5), the Edit Sequence tab (6), and the Optimize Sequence tab
(7).
 The Enter Sequence tab allows you to enter the sequence and provide all basic
information.
 Enter your sequence name (8), choose whether to enter a DNA or protein sequence (9),
and paste your sequence into the tool (10). If you enter a protein as an amino acid
sequence, it is important to optimize it for your desired expression host.
 Editing and optimizing your gene works in the same way as for full gene synthesis. For
details, see chapter 2.3, “Editing and Optimizing a Sequence”.
 The Sequence Summary tab (11) displays an overview of the actual sequence and all
edits made. The sequence and the summary can be downloaded. For more details, see
chapter 2.4, “Sequence Summary”.
 If you have entered all information, “Save and Close” the form (12).
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 The green check mark status indicator should now appear (13). If you are ready to place
your order, click “Proceed To Summary” (14).
 The next step is to add the project to your cart and proceed to checkout. Please read the
“Adding a Project to Cart and Placing Your Order” tutorial in chapter 7 to see how that
process works.
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4.2. MultiSequence Batch Upload for Strings™ DNA Fragments
 If you intend to order multiple sequences at the same time, the MultiSequence Batch
Upload (15) can make your ordering process faster.
 To get started, drag and drop the MultiSequence Batch Upload icon (15) onto the drop
area (16).
 Select “Strings™ DNA Fragments” (17). The next step is to choose whether to enter DNA
or protein sequences (18). If you enter a protein as an amino acid sequence, it is
important to optimize it for your desired expression host. In either case, you’ll need
FASTA-formatted sequences ready for pasting into the sequence field.
 In this example we’ve entered two DNA sequences (19).
 Then click “Save” (20).
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 The green status indicators should be showing (21).
 Click the “Proceed To Summary” button (22). Then you can add the project to your cart
and proceed to checkout. Please read the “Adding a Project to Cart and Placing Your
Order” tutorial in chapter 7 to see how that process works.
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5. Setup of Mutagenesis: Single Gene Variants
5.1. Which Sequence Qualifies as a Single Gene Variant?
 If you want to study a construct with mutations introduced by site-directed mutagenesis,
or a variant of your gene, begin by determining which sequence is the longest version of
your gene.
 You will need to order this sequence using the GeneArt® Gene Synthesis service (1), and
set up the shorter version as a variant (2).
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5.2. Single Gene Variant Project Setup
 To set up the synthesis of the longest version of your gene, drag and drop the Gene
Synthesis icon (3) onto the drop area (4).
 Next, work through the Gene Synthesis form by clicking the Gene Synthesis icon (5) and
entering the requested data and information. To learn more about completing the Gene
Synthesis form, please read chapter 2.
 Note that if you choose to optimize your sequence using the GeneArt® GeneOptimizer®
service, the variant sequence must be based on the optimized sequence.
 When you have completed the Gene Synthesis form, the green status indicator should be
shown (6).
 Now let’s see the process for ordering a single variant using the GeneArt® portal.
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 To set up your variant, drag and drop the Mutagenesis icon (7) onto the Gene Synthesis
icon in the Process area (8).
 The red exclamation mark lets you know that more information is needed (9).
 The next step is to open the Mutagenesis form by clicking the Mutagenesis icon (10).
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5.3. Entering Variant Sequence Information
 Enter a name for your sequence (11).
 The next step is to enter either a DNA (12) or protein (13) sequence.
Note that if you chose to optimize the sequence of the longest version of your gene when
setting up the initial gene synthesis, the variant must be based on the optimized
sequence. You can access the optimized sequence in the Gene Synthesis editor in the
Analysis Report in the “Sequence Summary” (14). Just click on this button (15) and
download or view the optimized sequence.
 Now we’ll return to the Variant form.
If you choose to enter the protein sequence (13) rather than the DNA sequence, your
project will require review by a GeneArt® scientist. You will receive an email when the
review is complete and then you can continue the ordering process.
 In this example we chose DNA. Copy and paste your variant sequence into the sequence
field (16).
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5.4. Additional Information
 If you plan to subclone your variant, enter the restriction sites you need in your sequence
(17). Note that the restriction sites must be present in the sequence you entered.
 If you need extra services, you can select them (18).
 Click “Save” (19).
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5.5. Information Complete
 The green status indicator should now be shown (20).
 Click the “Proceed To Summary” button (21). Then you can add the project to your cart
and proceed to checkout. Please read the “Adding a Project to Cart and Placing Your
Order” tutorial in chapter 7 to see how that process works.
 We have now used the Mutagenesis (Single Gene Variant) form to create a single variant.
 If you need several variants for your sequence, learn how to order them at the same time
by reading the “Mutagenesis Bundles” tutorial in chapter 6.
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6. Setup of Mutagenesis Bundles (Gene Variants)
5.1. Which Sequences Qualify as Mutagenesis Bundles?
 If you want to study mutations introduced by site-directed mutagenesis, or variants of
your gene, begin by determining which sequence is the longest version of your gene.
 You will need to order this sequence using the GeneArt® Gene Synthesis service (1), and
set up the shorter versions as variants (2).
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6.2. Mutagenesis Bundle Project Setup
 To set up the synthesis of the longest version of your gene, drag and drop the Gene Synthesis
icon (3) onto the drop area (4). Next, work through the Gene Synthesis form by clicking the
Gene Synthesis icon (5) and entering the requested data and information. To learn more
about completing the Gene Synthesis form, please read the “Gene Synthesis and Online
Optimization” tutorial in chapter 2.
 Note that if you choose to optimize your sequence using the GeneArt® GeneOptimizer®
service, all variant sequences must be based on the optimized sequence.
 When you have completed the Gene Synthesis form, the green status indicator should be
shown (6).
 Now let’s see the process for ordering multiple variants using the GeneArt® portal.
 If you want to order several mutants or variants, drag and drop the Mutagenesis Bundle
icon (7) onto the Gene Synthesis icon in the Process area (5).
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6.3. Entering Several Gene Variant Sequences
 Choose whether to create variants using the parent sequence service (8) or by uploading
variant sequences using a multifasta form (9).
 If you choose the parent service, the sequence used for the initial gene synthesis will be
copied to the number of variant forms you specify. You can then introduce the variations
you need into each Variant form.
 If you choose the multifasta form (9) you can paste all your variants into the system in
one step.
 First we will set up two variants using the multifasta form. Choose the multifasta option
(9).
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6.3.1. Setting Up a Mutagenesis Bundle With the Multifasta Option
 The form expands, and you can enter your prepared variants. Just copy and paste
multifasta-formatted mutagenesis sequences (10) into the sequence field in the portal
(11).
 If you need extra services, you can select them under “Add service” (12).
 Click “Save” (13).
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 The program has created three single variants (14).
 Open the first Mutagenesis form by clicking the icon (15).
 The name (16) and sequence field (17) are already filled in. You can change the name if
you desire. Finish completing the form as described in the “Mutagenesis” tutorial in
chapter 5.
 Click “Save” when you are finished (18).
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 The green status indicator should be shown now”.
 Complete the remaining variants in the same way (19).
 Once the form for every variant is completed, click “Proceed To Summary” (20) and send your
project to GeneArt® scientists for review. Within 24 hours, the GeneArt® team will review your
project and send you an email notification.
 When you receive a response from the GeneArt® team, you can add the project to your cart
and proceed to checkout. Please read the “Adding a Project to Cart and Placing Your Order”
tutorial in chapter 7 to see how that process works.
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6.3.2. Setting Up a Mutagenesis Bundle With the Parent Service Option
 Now we’ll set up two variants using the parent service rather than the multifasta method.
Go back to the step where gene synthesis for the longest version of the gene has been set up.
Drag and drop the Mutagenesis Bundle icon (21) onto the Gene Synthesis icon in the Process
area (22).
21
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 Choose the parent service option (23) and type in the desired number of variants—two in this
example. Then click “Save” (24).
 The program has created two single variants (25).
 Open the first form by clicking the icon (26).
23
26
25
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 The names of the parental sequence and the variant sequence are already filled in.
 You can change the name if you wish—in this example, we’ve chosen the name “Variant parent
1” (27).
 Now type in your desired sequence modifications (28).
 Finish completing the form as described in the “Mutagenesis” tutorial in chapter 5.
 Click “Save” (29).
27
29
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 The green status indicator should be shown now.
 Complete the remaining variants in the same way (30).
 Once the form for every variant is completed, click “Proceed To Summary” (31) and send your
project to GeneArt®
scientists for review. Within 24 hours, the GeneArt®
team will review your
project and send you an email notification.
 When you receive a response from the GeneArt® team, you can add the project to your cart
and proceed to checkout. Please read the “Adding a Project to Cart and Placing Your Order”
tutorial in chapter 7 to see how that process works.
30
31
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7. Adding a Project to Cart and Placing Your Order
7.1. Proceed to Project Summary
 Once you have completed defining your project, you can order GeneArt® services by
clicking “Proceed To Summary” (1).
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7.2. Project Summary
 The Project Summary page is displayed. Here you can download your overall project
report (2).
 Detailed files specific to different GeneArt® services are also available for download (3).
 Choose Express or SuperSPEED Service if required (4).
 If you are ready to check out, click the “add to cart” button (5) to proceed.
2
3
5
4
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 Note: If your project requires review by GeneArt® scientists (yellow status indicator), the
project summary will look different. Click “send for review” (6) to start the process. The
average review time is one business day. Once completed, your sequence will be placed in
your project folder and you can then proceed to order your gene as described above.
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7.3. Cart View
 You’ll see detailed information about your order, including the estimated processing time
(7). You can edit your project (8), or remove the project from your cart (9). If you do this,
the project will remain as a “draft” in your project folder, and you can place it in the cart
later. The price of your project is also displayed in the cart (10).
 The note at the top of the page is a reminder that you can have only one project in the
cart at a time, but a project can include as many sequences as you wish (11).
 Check this page carefully, and if the information is correct, click “Begin Checkout” (12).
7
9 10
11
12
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7.4. Shipment & Payment Information
 Please provide all of the required shipping and billing information in the upper part of the
page.
 A price update with handling charge is shown (13).
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 In the lower part of the page select a payment method.
 Please note: If you choose to pay with a purchase order and you don’t have a PO number,
please enter an internal reference number (14)
 UK and Italian purchasers: please confirm your VAT exemption, if applicable.
 Read the GeneArt® Terms and Conditions, and agree by ticking the box (15).
 Then click “Continue to Review” (16).
14
15
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7.5. Review Order
 The Review Order page gives a summary of all your order information: shipping, billing,
and payment information, your order details, and the total price of the order.
 Confirm the information, then click “Place My Order” (17).
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7.6. Order Confirmation 
 After placing your order, an “Order Confirmation” page will appear with all of the details.
You can print this page for your records (18).
 You will also receive a confirmation email.
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8. Contacts at GeneArt
For further information or if you have questions, please don’t hesitate to contact us at:
Email: geneartsupport@lifetech.com
Europe:
Phone: +49 (0)941 942 76-100
Fax: +49 (0)941 942 76-780
America and Asia:
Phone: 800-955-6288, option 4/4/1
For Research Use Only. Not for use in diagnostic procedures.
© 2013 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life
Technologies Corporation and/or its affiliate(s)or their respective owners. GenBank is a registered trademark of the United
States Department of Health and Human Services.
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