http://www.nature.com/news/big-biology-the-omes-puzzle-1.12484?WT.ec_id=NATURE-20130228 ‘Omics discussion in Nature SYLICA Synthetic Biology – Bowater Feb 2013 SYLICA 2013 Bowater lectures Synthetic Biology & Nanotechnology: Tomorrow’s Molecular Biology? Bowater Lectures in Brno, Feb. 2013 4 lectures on linked topics will be delivered during the coming week: •Contemporary DNA Sequencing Technologies – 26/2/2013 @ 10:00 •Using ‘Omic Technologies to Investigate Gene Function – 26/2/2013 @ 14:00 •Biophysical Methods to Study Molecular Interactions – 27/2/2013 @ 10:00 •Synthetic Biology & Nanotechnology: Tomorrow’s Molecular Biology? – 28/2/2013 @ 10:00 SYLICA Synthetic Biology – Bowater Feb 2013 Nanotechnology & Synthetic Biology •Presentation will discuss two overlapping topics: ØNanotechnology ØSynthetic Biology (incorporating metabolic engineering and protein engineering) •These are emerging disciplines, covering vast areas of science – not just biology! •Here it is only possible to introduce the topics and provide some brief discussion of specific examples SYLICA Synthetic Biology – Bowater Feb 2013 •To ensure that we are all clear where the discussion should start, it is useful to include some definitions…. Nanotechnology •Nanotechnology…. ØLiterally defined as: Technology that is useful on the nanoscale – 1-100 nm (atom scale = 0.1 nm) ØFor biologists, this is more usefully defined as: manipulation of biological molecules/structures to produce useful materials or devices •Biological molecules used in such technology must be stable for their required use e.g. uses of proteins will provide different opportunities to nucleic acids •Requires collaboration of molecular biologists with experts in quantum physics, organic chemistry, surface science, computer science….etc. SYLICA Synthetic Biology – Bowater Feb 2013 The combination of engineering with biology to engineer living things to create novel: Fuels, Medicines , and Materials The overall aims are to solve the Grand Challenges of the 21st Century Synthetic Biology SYLICA Synthetic Biology – Bowater Feb 2013 [USEMAP] Explanation on Youtube: http://www.youtube.com/watch?v=rD5uNAMbDaQ DNA Nanotechnology •DNA is appropriate for nanotechnological methods for several reasons: ØIt is a (relatively) stable chemical, which exists in different forms (nucleotides, nucleic acids) ØAs a polymer it can form very long molecules ØIt has a well defined, repetitive structure Ø“Rules” for determining the structure are simple and well-understood ØWithin the molecule many atoms are available to form useful interactions/modifications SYLICA Synthetic Biology – Bowater Feb 2013 DNA Origami •During the 1980’s, studies of DNA highlighted that complex structures could be formed •Since these structures are stabilised by base pairs in the molecule, it became clear that the complex structures could be created using carefully-designed DNA sequences •Importantly, the complex structures can be built up from simpler molecules Ø SYLICA Synthetic Biology – Bowater Feb 2013 DNA Origami SYLICA Synthetic Biology – Bowater Feb 2013 Seeman, 2010, Ann. Rev. Biochem., 79, 65-87 DNA Origami SYLICA Synthetic Biology – Bowater Feb 2013 Seeman, 2010, Ann. Rev. Biochem., 79, 65-87 Seeman Fig 4.jpg DNA Origami SYLICA Synthetic Biology – Bowater Feb 2013 Seeman, 2010, Ann. Rev. Biochem., 79, 65-87 DNA Origami: Examples SYLICA Synthetic Biology – Bowater Feb 2013 Pinheiro et al., 2011, Nat. Nanotech., 6, 763-772 Applications of DNA Nanotechnology •Not just for creating beautiful pictures…. SYLICA Synthetic Biology – Bowater Feb 2013 Pinheiro et al., 2011, Nat. Nanotech., 6, 763-772 figure 6.jpg Synthetic Biology SYLICA Synthetic Biology – Bowater Feb 2013 Synthia: a Synthetic Bacterium SYLICA Synthetic Biology – Bowater Feb 2013 Gibson et al., 2010, Science, 329, 52-56 •This paper reported the design, synthesis, and assembly of the 1.08–mega–base pair Mycoplasma mycoides JCVI-syn1.0 genome •The genome was chemically synthesised and transplanted into a M. capricolum recipient cell •The new M. mycoides cells are controlled by the synthetic chromosome, which also includes “watermark” sequences, designed gene deletions and polymorphisms, and mutations acquired during the building process •The new cells have expected phenotypic properties and are capable of continuous self-replication Expression Systems •Most widely used system to express recombinant proteins is E. coli •Need: -Expression vector (plasmid) -Specific bacterial strains •Many specialised expression systems and strains have been developed • SYLICA Synthetic Biology – Bowater Feb 2013 Bacterial Expression Strains •Number of different bacterial strains are in use •One of most widely used is E. coli BL21 •Takes advantage of a viral RNA polymerase, so will only express genes prepared downstream of viral promoter Novagen pET system manual, 11th ed •Strains altered to allow different types of control of gene expression •Strains also produced that allow expression of different types of genes SYLICA Synthetic Biology – Bowater Feb 2013 Codon Bias •Different organisms have differences in their usage of (A+T) and (G+C) •Leads to different biases in their use of codons and anti-codons •Strains of E. coli BL21 have been developed that can make tRNAs to allow them to cope with variation in codon usage e.g. Origami, Rosetta, etc. Escherichia coli http://www.kazusa.or.jp/java/codon_table_java/ http://www.kazusa.or.jp/java/codon_table_java/ Humans SYLICA Synthetic Biology – Bowater Feb 2013 Other Expression Strains •E. coli expression systems are very powerful but sometimes have problems •A better approach can be to try to express protein in native cell (or something similar) -Different types of bacteria -Yeast are widely used e.g. Pichia pastoris -Insect cells in culture -Mammalian cells in culture SYLICA Synthetic Biology – Bowater Feb 2013 Metabolic Engineering •Metabolic Engineering – or “Biotransformations” – relates to use of biological catalysts to produce specific, desired products •Usually enzymes, but can be whole organisms •Industry uses this to produce food, pharmaceuticals, detergents, agricultural chemicals, etc. • SYLICA Synthetic Biology – Bowater Feb 2013 Process Development Genetic engineering Protein engineering Product concentration Volumetric productivity •Quality •Purity •Scale Substrates Screening & selection of biocatalysts Biocatalyst production (fermentation/purification) Medium engineering Design & scale-up of bio-reactor Product isolation & purification Product Basic Biotechnology, 2006, Ratledge & Kristiansen, 3rd edn, Fig. 24.1 SYLICA Synthetic Biology – Bowater Feb 2013 Metabolic Engineering - Advantages •Use of enzymes/organisms has number of possible advantages compared to what can be achieved by chemical industry: -Simpler -Less raw materials and energy -Higher quality products -Higher yields -Decrease toxic wastes and wastewater -Lower costs and environmentally friendly?? SYLICA Synthetic Biology – Bowater Feb 2013 Compounds Produced by Commercial-scale Bioprocesses •Alcohols •Amino acids •Antibiotics •Polymers -Starch -Polyurethane •Sweeteners •Vitamins • SYLICA Synthetic Biology – Bowater Feb 2013 Prokaryotes used in Biotransformations •Wide range of prokaryotes used in biotransformations, including: ØEscherichia coli: Gamma-proteobacteria; widely used in development processes, produce amino acids ØMycobacterium spp: Actinobacteria; various agricultural and medical compounds ØRhodococcus rhodochrous: produces acrylamide ØStreptomyces coelicolor: Actinobacteria; antibiotics + wide range of other metabolites SYLICA Synthetic Biology – Bowater Feb 2013 •A number of diverse bacteria used as models for metabolic engineering •Microbial genome sequences have revealed many examples of ‘cryptic’ or ‘orphan’ biosynthetic gene clusters •Have potential to direct the production of novel, structurally complex natural products •Synthetic biology will provide new mechanisms, roles and specificities for natural product biosynthetic enzymes SYLICA Synthetic Biology – Bowater Feb 2013 Prokaryotes used in Biotransformations Tagged Proteins •“Tags” are widely used to give convenient, fast purification of recombinant proteins (via affinity chromatography) •One “tag” is Glutathione-S-transferase (GST) •GST is a small enzyme that binds glutathione (Glu to which Cys-Gly is attached) •GST tags are usually placed at C-terminus of proteins SYLICA Synthetic Biology – Bowater Feb 2013 Different Types of Tags SYLICA Synthetic Biology – Bowater Feb 2013 Fluorescently-tagged Proteins •Combination of molecular and cell biological studies analyse in vivo localisation of proteins expressed with a fluorescent “tag” •Important that “tag” does not interfere with protein activity • • • • Bastiaens & Pepperkok (2000) TiBS, 25, 631-637 •Can examine localisation of proteins containing different fluorophores SYLICA Synthetic Biology – Bowater Feb 2013 GFP–Tagged Protein Localization SYLICA Synthetic Biology – Bowater Feb 2013 FIGURE 9–16 Green fluorescent protein (GFP). (a) The GFP protein (PDB ID 1GFL), derived from the jellyfish Aequorea victoria, has a β-barrel structure; the fluorophore (shown as a space-filling model) is in the center of the barrel. (b) Variants of GFP are now available in almost any color of the visible spectrum. (c) A GLR1-GFP fusion protein fluoresces bright green in Caenorhabditis elegans, a nematode worm (left). GLR1 is a glutamate receptor of nervous tissue. (Autofluorescing fat droplets are false colored in magenta.) The membranes of E. coli cells (right) are stained with a red fluorescent dye. The cells are expressing a protein that binds to a resident plasmid, fused to GFP. The green spots indicate the locations of plasmids. Biotechnological Applications •Protein engineering approaches can be used to provide significant alterations to cell function •Such changes bring forward important ethical and moral issues that need to be addressed SYLICA Synthetic Biology – Bowater Feb 2013 Medical Applications •Protein engineering is also finding increasing uses in human medicine •Again, such uses have important ethical and moral issues that need to be addressed SYLICA Synthetic Biology – Bowater Feb 2013 Protein Engineering •In all cells proteins have: -Enzyme activities -Structural roles •In past 50 years scientists have learned how to prepare large amounts of pure proteins •Allows detailed in vitro studies •Proteins can also be made to do useful operations both in vitro and in cells •Protein engineering involves processes that modify or improve proteins SYLICA Synthetic Biology – Bowater Feb 2013 Improving Proteins •Quite difficult to improve on activities of proteins for any particular cell – evolution is very efficient! •Can replace mutated (dysfunctional) proteins •Recent advances have tried to make use of novel or uncommon amino acids -Selenocysteine: in a few proteins in all cells (e.g. formate dehydrogenase in bacteria, glutathione peroxidase in mammals) -Pyrrolysine: found in methanogenic group of Archaea • SYLICA Synthetic Biology – Bowater Feb 2013 Uncommon Amino Acids •Expansion of genetic code to uncommon amino acids requires several changes in cells: -Specific aminoacyl-tRNA synthetase -Specific tRNA -New metabolic pathways (??) for synthesis of above molecules • • •Scientists have used similar approaches to incorporate unnatural amino acids •Added one at a time, but over 30 different amino acids have been introduced •Performed for E. coli, yeast, mammalian cells (a) ketone; (b) azide; (c) photocrosslinker; (d) highly fluorescent; (e) heavy atom for use in crystallography; (f) long-chain cysteine analogue SYLICA Synthetic Biology – Bowater Feb 2013 Tomorrow’s Molecular Biology: Overview •Biology offers a range of molecules that can be manipulated to produce useful materials or devices •Synthetic biology incorporates “engineering” approaches to take advantage of and improve biological systems to tackle specific problems •Protein Engineering manipulates protein production, incorporating modifications to “improve” proteins •Recombinant proteins can provide much information about protein function both in vitro and in vivo •Engineered proteins have huge potentials in biotechnology and medicine •Important ethical and moral issues to overcome SYLICA Synthetic Biology – Bowater Feb 2013 A Global Synthetic Biology Competition for Undergraduate Students iGEM: What is it? SYLICA Synthetic Biology – Bowater Feb 2013 iGEM: International Genetically Engineered Machines Organised by the iGEM Foundation, a spin out from the MIT in the USA SYLICA Synthetic Biology – Bowater Feb 2013 What is Involved in iGEM? •The team (Students + Advisers) develop a Synthetic Biology Project that must be completed during the summer months •Teams compete to win medals (Gold, Silver or Bronze) and prizes • •Genetic engineering must be perfromed within the project, following quite strict criteria •Also must involve Human Practices (outreach) and consideration of ethical issues related to the project Find out what we did: Facebook: www.facebook.com/UEAJIC.IGEM Twitter: www.twitter.com/UEAJIC_IGEM Wiki: http://2011.igem.org/Team:UEA-JIC_Norwich In October 2011 the UEA-JIC_iGEM Team attended the iGEM Jamboree in Amsterdam NRP-UEA iGEM Team 2012 In 2012 we organised the 2nd iGEM team based on the Norwich Research Park For info: see http://2012.igem.org/ Team:NRP-UEA-Norwich Our team included 7 undergraduate students from BIO We were predominantly based at the School of Biological Sciences at UEA