Česká společnost chemická, pobočka Brno
Vás zve na přednášky, která se konají v místnosti 132, 1.NP, budova
A11, Kamenice 5, Přírodovědecká fakulty Masarykovy univerzity, Brno
ve čtvrtek 2. května 2013 v 14:00 hod.
své příspěvky přednesou
DOC. JANA ROITHOVÁ
DEPARTMENT OF ORGANIC CHEMISTRY, CHARLES UNIVERSITY IN PRAGUE
Mass spectrometry in organic chemistry:
More than just a classical tool
The combination of electrospray ionization and mass spectrometry is often used for
investigation of reaction mixtures. In favourable cases, the reactive intermediates can be
transferred to the gas phase and their uni- or bimolecular reactivity can be studied. Infrared
multiphoton dissociation (IRMPD) spectroscopy allows obtaining infrared spectra for the
mass-selected ions and hence the elucidation of the ion structures. This approach can bring a
new insight into many reactions proceeding via ionic intermediates. The lecture will be
devoted to the presentation of the potentials of the gas-phase methods and their application to
several organometallic reactions.
DOC. MICHAL HOCEK
INSTITUTE OF ORGANIC CHEMISTRY AND BIOCHEMISTRY AS CR
AND
DEPARTMENT OF ORGANIC CHEMISTRY, CHARLES UNIVERSITY IN PRAGUE
Base-modified nucleosides and nucleotides as novel cytostatics
and building blocks for polymerase synthesis of modified DNA
Novel methodologies of synthesis of modified nucleobases, nucleosides, nucleotides and
oligonucleotides have been developed largely using modern organometal-catalyzed reactions
(cross-couplings, C-H activations, etc.). An efficient two-step methodology of construction of
functionalized nucleic acids was developed by a novel chemo-enzymatic approach using
aqueous-phase cross-coupling reactions of nucleotides followed by incorporation by DNA
polymerase. The methods are applied in the synthesis of derivatives for biological activity
screening (two novel types of nucleoside cytostatics will be presented) and the use of
functionalized oligonucleotide probes for electrochemical or fluorescent detection of DNA,
for DNA bioconjugation or regulation of protein binding.