PRODUCT INFORMATION
Thermo Scientific
GeneRuler DNA
Ladder Mix
#SM0331 250 (5 x 50) µg
(for 500 applications)
Lot: _
Concentration: 0.5 µg/µL
Supplied with: 2 x 1 mL 6X DNA Loading Dye
Store at -20°C
In total 7 vials.
www.thermoscientific.com/onebio
Description
Thermo Scientific GeneRuler DNA Ladder Mix is designed
for sizing and approximate quantification of wide range
double-stranded DNA on agarose gel. The ladder is
composed of 21 chromatography-purified DNA fragments
(in base pairs): 10000, 8000, 6000, 5000, 4000, 3500,
3000, 2500, 2000, 1500, 1200, 1000, 900, 800, 700,
600, 500, 400, 300, 200, and 100. It contains three
reference bands (3000, 1000 and 500 bp) for easy
orientation.
The ladder is dissolved in TE buffer.
Storage Buffer
10 mM Tris-HCl (pH 7.6), 1 mM EDTA.
6X DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue,
0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA.
CERTIFICATE OF ANALYSIS
Well-defined bands are formed during agarose gel
electrophoresis.
The DNA concentration is determined
spectrophotometrically.
The absence of nucleases is confirmed by a direct
nuclease activity assay.
Quality authorized by: Jurgita Zilinskiene
Rev.7 V
Protocol for Loading
Loading mixture for the 5 mm agarose gel lane*:
DNA Ladder 1 µL
6X Loading Dye Solution 1 µL
Deionized water 4 µL
6 µL
Step 1: Mix gently
Step 2: Load on the gel
* For gels with other lane widths, the components of the mixture should be
scaled either up or down. Use 0.2 µL (0.1 µg) of DNA Ladder per 1mm of lane.
Recommendations
• Do not heat before loading.
• Dilute your DNA sample with the 6X Loading Dye Solution
(#R0611, supplied with the ladder): mix 1 volume of the dye
solution with 5 volumes of the DNA sample;
• Load the same volumes of the DNA sample and the DNA ladder;
• For quantification, adjust the concentration of the sample to
equalize it approximately with the amount of DNA in the nearest
band of the ladder.
• For DNA band visualization with SYBR
®
Green, GelRed and
other intercalating dyes, do not add the dyes into the sample,
use gel staining after electrophoresis or include dyes into
agarose gel to avoid aberrant DNA migration.
PRODUCT USE LIMITATION
This product is developed, designed and sold exclusively for research purposes and in vitro use only. The
product was not tested for use in diagnostics or for drug development, nor is it suitable for administration
to humans or animals. Please refer to www.thermoscientific.com/onebio for Material Safety Data Sheet of
the product.
© 2012 Thermo Fisher Scientific Inc. All rights reserved. SYBR is a registered trademark of Molecular
Probes, Inc. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.
GeneRuler DNA Ladder Mix
NOTICE TO PURCHASER: LIMITED LICENSE
This product or its use is covered by at least one claim of U.S. Patent Nos. 5,834,201, 6,680,378, and/or
7,132,520 owned by Invitrogen Corporation. The purchase of this product conveys to the buyer the nontransferable
right to use the purchased amount of the product in internal research conducted by the
buyer. The buyer cannot use this product or materials made by the employment of this product for
Commercial Purposes. Commercial Purposes means any activity for consideration and may include, but is
not limited to: (1) use of the product in manufacturing; (2) use of the product to provide a service,
information, or data; (3) use of the product for therapeutic, diagnostic or prophylactic purposes; or (4)
resale of the product, whether or not the product is resold for use in research. Further information on
purchasing licenses under the above patents may be obtained by contacting the Business Development
Department, Invitrogen Corporation, 5791 Van Allen Way, Carlsbad, CA 92008. Email:
outlicensing@invitrogen.com.