c (ng/ul)	log c				GRAF		
					log c	GAPDH	JUN
						17.32453333	24.4929
						19.48663333	26.02606667
						22.52283333	28.88226667
						25.50846667	31.61166667
						28.94756667	35.04293333
						31.24963333	38.26156667
							
Bez činidla							
GAPDH		JUN					
Vzorek	Ct	Vzorek	Ct				
undil	17.3938	undil	24.5394				
undil	17.2411	undil	24.6273				
undil	17.3387	undil	24.312				
Æ	17.32453333	Æ	24.4929				
10x	19.4557	10x	26.1271				
10x	19.1798	10x	25.7467				
10x	19.8244	10x	26.2044				
Æ	19.48663333	Æ	26.02606667				
100x	22.3733	100x	28.7587				
100x	22.548	100x	28.993				
100x	22.6472	100x	28.8951				
Æ	22.52283333	Æ	28.88226667				
1000x	25.2486	1000x	31.1467				
1000x	25.5823	1000x	31.8381				
1000x	25.6945	1000x	31.8502				
Æ	25.50846667	Æ	31.61166667				
10 000x	28.5435	10 000x	34.094				
10 000x	29.1017	10 000x	35.0348				
10 000x	29.1975	10 000x	36				
Æ	28.94756667	Æ	35.04293333				
100 000x	31.2691	100 000x	37.8332		a)	GAPDH	
100 000x	31.1358	100 000x	37.705				
100 000x	31.344	100 000x	39.2465			E = (10^-1/k - 1) x 100 =	
Æ	31.24963333	Æ	38.26156667				
							
					b)	JUN	
Kontroly							
NTC		noRT				E = (10^-1/k - 1) x 100 =	
GAPDH	JUN	GAPDH	JUN				
Undetermined	Undetermined	Undetermined	27.8936				
Undetermined	Undetermined	Undetermined	27.9149				
							
							
##### Sheet/List 2 #####
Bez činidla					DMSO										
GAPDH		JUN			GAPDH		JUN								
Vzorek	Ct	Vzorek	Ct		Vzorek	Ct	Vzorek	Ct							
cDNA	19.2368	cDNA	26.2645		cDNA	19.6746	cDNA	26.3573							
cDNA	19.1108	cDNA	26.0897		cDNA	19.7816	cDNA	26.3632							
cDNA	20.6262	cDNA	27.5725		cDNA	19.6535	cDNA	26.3279							
Æ	19.65793333	Æ	26.64223333		Æ	19.70323333	Æ	26.34946667							
EtOH 1µl	19.9144	EtOH 1µl	27.8717												
EtOH 1µl	19.5741	EtOH 1µl	27.7677												
EtOH 1µl	20.0881	EtOH 1µl	29.9076												
Æ	19.85886667	Æ	28.51566667												
"EtOH 0,5µl"	21.2638	"EtOH 0,5µl"	35.6947												
"EtOH 0,5µl"	32	"EtOH 0,5µl"	Undetermined												
"EtOH 0,5µl"	Undetermined	"EtOH 0,5µl"	Undetermined		Vzorek	JUN	GAPDH	Δ Ct	ΔΔ Ct	R					
Æ	26.6319	Æ	35.6947		bez činidla	26.64223333	19.65793333	6.9843	0	1					
					DMSO	26.34946667	19.70323333	6.646233333	-0.338066667	1.26					
															
															1 bez činidla
															2 DMSO
															
Bez činidla					DMSO										
GAPDH		JUN			GAPDH		JUN								
Vzorek	Ct	Vzorek	Ct		Vzorek	Ct	Vzorek	Ct							
cDNA	20.0249	cDNA	27.1316		cDNA	19.9764	cDNA	25.985							
cDNA	19.6426	cDNA	26.7751		cDNA	19.586	cDNA	25.673							
cDNA	19.5989	cDNA	26.8056		cDNA	20.1762	cDNA	26.2685							
Æ	19.75546667	Æ	26.9041		Æ	19.91286667	Æ	25.9755							
EtOH 1µl	36.4963	EtOH 1µl	Undetermined												
EtOH 1µl	22.0917	EtOH 1µl	Undetermined												
EtOH 1µl	29.8261	EtOH 1µl	Undetermined												
Æ	29.47136667	Æ	Undetermined												
"EtOH 0,5µl"	19.5372	"EtOH 0,5µl"	27.3939												
"EtOH 0,5µl"	19.2463	"EtOH 0,5µl"	27.6701												
"EtOH 0,5µl"	19.3838	"EtOH 0,5µl"	27.6298		Vzorek	JUN	GAPDH	Δ Ct	ΔΔ Ct	R					
Æ	19.3891	Æ	27.5646		bez činidla										
					DMSO