BIOINFORMATIKA V PRAXI CVIČENÍ 3 – DRUHÁ ČÁST IDENTIFIKACE GENŮ, PROTEINŮ A JEJICH FUNKCE STUDIJNÍ MATERIÁLY Studijní materiály předmětu C2130 Úvod do chemoinformatiky a bioinformatiky, přednáška Predikce genu, Sequence-evolution-function: Computational Approaches in Comparative Genomics. CHYBY PŘI PREDIKCI GENŮ Velmi zjednodušený přístup k predikci prokaryotických genů (genem je nejdelší ORF) vede k chybám, ale jejich množství je poměrně malé. Chyby mohou také vznikat při sekvencování DNA. Přidání/odstranění startovního a/nebo stop kodonu může vést ke zkrácení, prodloužení nebo úplnému vynechání genu. ÚKOL 1 – příklady chyb vzniklých při sekvenaci Pomocí predikčního programu GeneMark (http://exon.gatech.edu/GeneMark) identifikujte geny v sekvenačních výstupech a porovnejte je s původní sekvencí z databáze (část genomu E. coli). Určete, k jaké chybě došlo. Pro porovnání rozdílně predikovaných genů využijte program EMBOSS Needle (http://www.ebi.ac.uk/Tools/psa/emboss_needle/nucleotide.html). SEKVENCE E. COLI TTAAGAAATTCCGGTTATCAACTTCGAGGCCCCTTTCAGGTCACCGTTGGCGTATTTCGGGGCGATAGATTTGTATTTGTAGCCGCGAATACTGCGATC GCCCCCGGCGAAAAAACGCAGATCCGGCGGTACTTTGTCGAAATCACCGGTTTCAATCCAGCCCAGCGTGCCGCGTGTAACGAAACGATGGCGATCGTA CAGTGTGCGGATCCAGACGTTCTGCGCCTGGAAAACGGAGAAATCGACATCTGAGCCCCAGGCCGTGTTGGAGTAGTCGATAGAGTAGCGTTGCGAGTC GCCCCTGGTTGGCATCAGGCCACCACGAGAACGCGTGCGGCTAATCATCACCCCAGGATAAAACAGCATCGTGGTGTTGGTAATTTCACCCTGAGTAAA GTGGTCGAGACTCCAGCGCAGGTTAATGGCACGCTGCCAGCCGCTGGAGAGATCCCAGTAGCGAGAAGCCACCAGCGTAGTGGAGTCAGATTCGGTATC GTTCAGGTCAGTGCGCTTAAAACCGCCCTGCACCAAATAATATTGTTCCAGTGGATTCTTCAGCAGCGGCATTTTATAGCTGAAGTCGAGGGTCTGTTC CGGCGCGGAAATACTGGTACTGGTGGTCAGACTGTGACCATAAGAGTTCATCCACGGCTTTTTCCACGTAGCTTTCACGCGCGGTCCCACGTCCGTAGA GTAACCGACCCCGGTTTCGATGGTGTTTTCAGTTCGCGGCGAAACCACGCCCGTCAAGGGTAATACTTTCGTTTCGCGCGCTTTATCAAATTGTGGAGC CACTACGACCGAGTTAAACCAGCCGGTAGCAGAAAGTCGACGGTTAAGTTCCGCCAGATCTTTCGATTCGTACTCATCGCCCTCTTTAAACGGCACCAG ATTTTGCAGGTATTCATCGCGGATTTGTGATCCTTCAAAGGTCACATGCCCAAAGCGGTAACGTTCGCCACTGTTATAATCAATATCCCAGAAGGCTTT ATGCAGGCCGAGCGCAATGCCCAGCTGCGCTTTGGTAAATTCGCTATCGAAATAACCTTTACGCAACGCAATGCTGGTTAAGGACTTTTTGAAATTTTC ATAATCGCCCTGGTTCAGTACCGTGCCAATAGCCGGGCGAGTATCGAGCAATTTCAAATAGTCTTTATCGGTCCGCGCGCCGCCGCGCAATACCACATC GGTGCCGCCAATTAACACCGGCACGCCTGGCGTGACTTTGGCGATCAATACCTGCCGCCCTTTCTTTGGCGGTGGACGGAGATCAAATTCAATGGTCGG CTGGTAATAACCCAGCGCTTTCAGACCTTCGCGGATGGCATCATCGACGCGTGCGCGAAAGCGACGGTCTGGCGTCACTTCATCACTTTCAATCGTAGA AAGCTGCGCACGAACGTTCTTTTCCAGCTGTCCCGATAACCCCTCGACCTGTAGACGGACGTTCGCGGCGACGGCAGATCCGCTTAAGCAGAGTAAGCT TACACAGCATAACTGTCGGATATAGCGCATATTTTCTCCTGAATATCCTTTTTTCCTGCCCCTGGAACGCCGTTAAACGGCTTAACAAAAATCCAGTAA TATGGATTAAAAAGCAGACTAAACCCCAAATATTTCTTATGTTTTACTTTAGACCTATTCACGGTGGTTATTGTGTGCAAATACGCCTCTTGTTACAAC CTTAACCCCAATGACCGATTTTCGGGAGAGCGACACCATGAGTTTATTTGATAAAAAGCATCTGGTTTCCCCCGCCGATGCCCTGCCTGGACGTAACAC CCCGATGCCCGTAGCCACGCTGCATGCGGTCAACGGTCACTCAATGACCAATGTACCTGACGGAATGGAGATTGCCATTTTTGCGATGGGTTGTTTCTG GGGTGTGGAGCGTCTGTTCTGGCAGTTACCCGGCGTTTACAGCACCGCCGCAGGCTATACCGGCGGCTATACGCCAAATCCGACTTATCGGGAAGTGTG CTCCGGTGATACGGGTCATGCCGAAGCGGTACGCATTGTTTACGATCCTTCTGTCATCAGCTATGAGCAGTTGCTACAGGTATTTTGGGAGAATCACGA TCCCGCCCAGGGAATGCGTCAGGGCAATGACCACGGCACGCAGTATCGTTCAGCGATTTATCCGCTGACCCCAGAACAGGATGCCGCAGCTCGCGCCAG TCTGGAACGTTTTCAGGCGGCGATGCTTGCCGCCGATGACGATCGTCACATCACCACGGAAATCGCTAACGCCACACCGTTTTATTATGCCGAAGATGA CCACCAGCAATATCTGCATAAAAACCCGTATGGTTACTGTGGAATTGGCGGAATTGGCGTCTGTCTGCCACCGGAAGCATAGCGTTACGGGTACAAATG TAGATTGTTGATAAAGTGCGTTTTGTTTATGCCGAATGCAGCGTGAATGCCTTACCAGGCCTACAAAATCGTCCAAATTCAATATATTGCAGGGACTGC GTAGGCCTGATCGGCATAGCGCATCAGGCAGTATTGCATTTATCAGCAGTCTGAATTTTAACCCTCTGGCGACTTTACAGTACCTTACGCTATACTAGC CACTGAAAATGCCGGATCACTTTCTTCGAATCGGCTTTCAATGTGTATTTCACACAAATTAATCAACTTCCCCTTCCGAGGATCTGGCCTGAAAGGTCG GATAAGATATGTTAAACAGTATTTTAGTCATACTCTGCTTGATCGCTGTAAGTGCGTTCTTCTCGATGTCTGAGATCTCACTTGCCGCCTCACGCAAAA TCAAACTTAAACTGCTGGCTGATGAAGGCAATATAAATGCCCAACGCGTTCTGAATATGCAGGAAAATCCCGGCATGTTCTTTACGGTGGTCCAAATCG GTCTGAACGCAGTTGCGATTCTCGGCGGTATCGTCGGTGATGCGGCATTTTCTCCAGCTTTTCACAGCCTGTTCTCCCGCTATATGTCGGCAGAACTCT CTGAGCAACTGAGCTTTATTCTCTCTTTCTCGTTAGTGACTGGCATGTTTATCCTGTTTGCGGATTTAACCCCGAAACGCATCGGTATGATTGCGCCAG AAGCGGTGGCTTTGCGTATCATCAACCCGATGCGCTTCTGCCTGTACGTTTGCACCCCGCTGGTGTGGTTCTTCAACGGCCTGGCGAACATGATCTTCC GTATTTTCAAACTGCCAATGGTACGTAAAGATGACATCACTTCTGATGACATCTACGCGGTAGTGGAAGCCGGTGCGCTGGCGGGCGTGTTACGTAAAC AGGAACACGAGCTGATTGAAAACGTCTTTGAGCTGGAATCCCGTACCGTTCCGTCTTCAATGACACCGCGTGAAAACGTGATTTGGTTTGATCTCCACG AAGATGAGCAAAGCCTGAAGAATAAGGTGGCGGAACATCCGCACTCTAAGTTCCTCGTCTGTAATGAAGATATTGACCACATCATCGGTTATGTCGATT CTAAAGACCTGCTGAACCGCGTGCTGGCTAACCAAAGCCTGGCACTGAACAGCGGCGTACAAATTCGCAACACGCTGATTGTGCCGGATACGTTAACCC TTTCAGAGGCGTTGGAAAGTTTTAAAACCGCAGGTGAAGACTTCGCGGTGATCATGAACGAGTACGCGCTGGTGGTGGGGATCATCACCCTCAACGACG TGATGACCACGCTGATGGGCGATCTGGTCGGTCAGGGGCTGGAAGAGCAGATTGTCGCCCGTGATGAGAACTCATGGCTGATTGACGGCGGCACCCCAA TTGACGACGTCATGCGCGTGCTGGATATTGACGAGTTCCCGCAGTCGGGCAACTACGAAACCATCGGAGGCTTTATGATGTTTATGCTGCGTAAGATCC CGAAACGCACCGATTCGGTGAAATTCGCCGGCTACAAATTTGAAGTGGTGGATATCGATAACTACCGCATCGACCAGCTGCTGGTGACCCGGATCGACA GCAAGGCCACCGCCCTTTCGCCAAAACTGCCTGACGCTAAAGATAAAGAAGAAAGCGTCGCGTAACCCCCAGAAACATCAACGGCTCCTGAATCAGGAG CCGTCTTATTACTGCATAGCACTTTGGTTAAGCCATCTCTGTTTGCAGACGCATAACCTGACGGTTAACTTCGGACATCACTGACAAATGCAGCTTATC CTTCACTTTTGGGATAAGAATCTTACCCTTATCAAATTCAAAAGCGCCAACGTCCTTGATGTATAACCGTCCACGGAACAGGATCTTTACGTACTTCGC CACCTGAAGTGGGTTGTACCGTTGGAAAATTTTCATTGTTATCTCCTGCTGAGTATTACGCCTGTGCGGTGCCACAATCGGCCCAACTATTATGAGGCG CAAATTTTAATGCCTAGTGACTATAGACTATCCGGGCAATGTTTCCACCGCGTATAACTTTTTTTACCTAAAGGTTACAATTATTCAGAATTATCTTTT TACCGAAGCGCGGTCTTCAGTATAAGCATTCATTTTTCATATGATTTGTGCGCTTGACCGCAAACTGGCATCACACTTGCGGGAAATTCGATAAATAGC ACATATGATTAAAACTCAGACCCAAGTGGTCGGATCACCTGCATATCATAAGAAGGAAACACCATGACCCTACGCAAGATTCTGGCACTCACCTGCCTG CTGTTGCCGATGATGGCTTCCGCACATCAGTTCGAAACCGGTCAGCGAGTGCCGCCGATTGGCATCACCGATCGTGGCGAGTTGGTGCTTGATAAAGAT CAGTTTAGCTACAAAACCTGGAACAGCGCGCAGTTAGTGGGAAAAGTGCGAGTACTGCAACATATTGCTGGTCGCACCTCTGCAAAAGAGAAAAACGCG ACGCTGATTGAAGCGATTAAATCAGCGAAGTTACCGCACGATCGTTACCAGACCACCACCATTGTTAACACCGACGACGCAATTCCGGGTTCCGGCATG TTTGTGCGCAGCAGTCTGGAGAGTAATAAAAAGCTTTATCCCTGGTCGCAGTTTATTGTTGATAGCAATGGCGTCGCACGCGGTGCCTGGCAGCTGGAT GAAAAAAGTTCCGCTGTAGTGGTGCTGGATAAAGACGGTCGCGTGCAATGGGCCAAAGACAGGGCGCTTACTCAGGAAGAGGTGCAGCAAGTGATGGAC CTGCTGCATAAATTAATTAATAAATAGACACTCTGAACCCAGGATTCAGGAACGACTCGCGCGGAGTGTAATCCAGCGGTTTACCCTGCCAGTCGTGAA CGTGCGCTCCGGCAGCTGCGGCCACAGCATGTCCAGCGGCGGTGTCCCAAATATTCGTTGGTCCGAAGCGCGGGTACAGCTGCGCCTGTCCTTCCGCCA CCAGGCAGAATTTCAGCGAAGAGCCGATGGACGTGGTCTGATGTTCGCCAAGCTGTTGCAGATACTCTTTCAGCTCCGCATCTGCATGGGAACGGCTGA TAACCACCAGCGGCGGGCGCGCATCGCGGACCTGAATCTGCTTGCGCACACCGCACTCTTCTTTCCAGGCCTTGCCTTCTGCCGCGCTGTACATTACGT TCATTACCGGCGCATACACCACACCTAAAATCGGTTTGCCATGGTCAATGAGCGCAATGTTAACGGTGAATTCGCCATTGCGTTTAATAAACTCTTTAG TACCATCCAGCGGGTCTACCAGCCAGTAACGCTGCCAGTGCTGACGGACTTCCCAACCGGGAGGATCTTCTTCAGAAAGGACCGGAATATCCGGTGTCA GCGTACGTAAACCGTCCATGATAACGGTGTGAGCGGCAATATCCGCTGCCGTTACCGGAGAATTGTCCGCTTTGCTGACGACGTCCATCGGTTTCGTCC CGTCGTAGACCTGCATAATGGCATCGCCTGCATTCCGTGCAAGCTGGCATACTTGATCTAACATTTCTCCACCTCGTCTCTGTGAGCGGTGTTAACTTA TTGTTTTACTTATACCCTATCGTTAATGAATGCGCCAACTGTGATAGTGTCATCATTTTCAAAGCGTAAAATTGTGGCATTCTTCACTGTTCTATAAGT AAGACGTTTATTCTTCCTTTTCTTTCGTATTCCCGATGATAAAAGGATGTCCCTGATGATTAAGTTTAGCGCAACGCTCCTGGCCACGCTGATTGCCGC CAGTGTGAATGCAGCGACGGTCGATCTGCGTATCATGGAAACCACTGATCTGCATAGCAACATGATGGATTTCGATTATTACAAAGACACCGCCACGGA AAAATTCGGACTGGTACGTACGGCAAGCCTGATTAACGATGCCCGCAATGAAGTGAAAAACAGCGTACTGGTCGATAACGGCGATTTGATTCAGGGGAG TCCGCTGGCCGATTACATGTCGGCGAAAGGATTAAAAGCAGGTGATGTTCATCCGGTTTATAAGGCGCTGAATACGCTGGATTATACGGTCGGTACACT CGGCAATCATGAATTTAACTACGGTCTGGATTACCTGAAAAATGCGCTGGCGGGAGCGAAATTCCCTTATGTAAATGCCAACGTCATTGACGCCAGAAC CAAACAGCCAATGTTTACACCGTATTTAATTAAAGATACCGAAGTGGTCGATAAAGACGGAAAAAAACAGACGCTGAAGATTGGCTATATTGGCGTCGT GCCGCCGCAAATCATGGGCTGGGATAAAGCTAATTTATCCGGAAAAGTGACGGTGAATGATATTACCGAAACCGTGCGCAAATACGTGCCTGAAATGCG CGAGAAAGGTGCCGATGTCGTTGTCGTTCTGGCGCATTCCGGGCTGTCTGCCGATCCGTATAAAGTAATGGCGGAAAACTCAGTTTATTACCTCAGTGA AATTCCGGGCGTTAACGCCATTATGTTTGGTCATGCTCACGCCGTTTTCCCGGGTAAAGATTTTGCTGATATCGAAGGGGCTGATATCACCAAAGGCAC GCTGAATGGTGTTCCGGCGGTAATGCCGGGCATGTGGGGCGATCATCTTGGGGTGGTCGACTTACAACTCAGTAATGACAGCGGTAAATGGCAGGTGAC GCAGGCGAAAGCGGAAGCTCGGCCGATTTACGACATCGCCAATAAAAAATCCCTCGCGGCGGAAGACAGCAAGCTGGTAGAAACACTCAAAGCCGATCA CGATGCCACACGCCAGTTCGTCAGCAAGCCAATCGGTAAATCTGCCGACAATATGTATAGCTATCTGGCGCTGGTGCAGGACGATCCGACCGTGCAAGT AGTGAACAACGCGCAAAAAGCGTATGTCGAACATTACATTCAGGGCGATCCGGATCTGGCAAAACTGCCGGTGCTTTCAGCTGCCGCACCGTTTAAAGT TGGTGGTCGCAAAAATGACCCGGCAAGCTATGTGGAGGTGGAAAAAGGCCAGTTGACCTTCCGTAATGCCGCCGATCTTTATCTCTACCCCAATACGCT GATTGTGGTGAAAGCCAGCGGTAAAGAGGTGAAAGAGTGGCTGGAGTGCTCCGCCGGACAGTTTAACCAGATTGATCCTAACAGCACGAAACCGCAATC ACTCATCAACTGGGATGGTTTCCGCACCTATAACTTTGATGTGATTGATGGTGTGAATTATCAGATTGATGTTACCCAACCTGCCCGTTATGACGGCGA GTGCCAGATGATTAATGCCAATGCGGAAAGGATTAAGAACCTGACCTTTAATGGCAAGCCGATTGATCCGAACGCCATGTTCCTCGTTGCCACCAATAA CTATCGCGCTTACGGCGGCAAATTTGCCGGTACGGGCGACAGCCATATCGCTTTTGCTTCACCGGATGAGAACCGCTCGGTGCTGGCAGCGTGGATTGC TGATGAGTCGAAACGTGCGGGGGAAATTCACCCGGCGGCAGATAACAACTGGCGTTTAGCACCGATAGCTGGCGATAAGAAACTGGATATCCGTTTCGA AACTTCTCCGTCAGATAAAGCCGCAGCGTTTATTAAAGAGAAAGGGCAATATCCGATGAATAAAGTCGCGACCGATGATATCGGGTTTGCGATTTATCA GGTGGATTTGAGTAAGTAAAACACTTCTTTTTCGGCCTATAAATCATCAACCGCATCCGGCATTTATTGGCGGATGCGATGCTGGCGCATCTTATCCGC CCTACAAGCCATGCACCGTAGACCAGATAAGCTCAGCGCATCCGGCAGTTATGCCGCACGTTCATCCCGCACCGCCAACACCTCGGGCAAATTCAACTC AATCCAGTCCGCCAGTGCAGCAACCTTTTCGCTCACCTGCTCGCCCAGCGGCGTGAGGCTATATTCCACATGCGGCGGCACCACCGGATACGCGATACG GTTAAGAAAACCATCCTGTTCTAACGCCTGTAACGACTGCGCAAGCATCTTTTCGCTCACCCCGCCGATTTTGCGCCGCAGGTCGCTAAAGCGATGAGT ACCTTCGCGTAGCGCCACCAGAATCAACACCCCCCAACGGCTGGTGACGTGTTTCAACACCTCGCGCGACGGGCACTGTTCCGCAAAGAGATTACCCTC TTTCAGTTGTTGCGACAGGCTAACCTGACTCATTTCATACTTACCTTTTTGTGCGTACTTACTAAAAGTAAGTTTAGGTGTTAGCGTATTTAAACACAA GACAAAACGATGGAGACTTCCCATGATCGCTATTACTGGTGCCACTGGCCAACTTGGTCACTATGTTATTGAATCCTTGAAGAAAACGGTTCCTGTCAG CCAAATAGTGGCTATCGTTCGTAATCCGGCAAAAGCCCAGGCTCTGGCAGCACAAGGCATTACCGTGCGTCAGGCTGACTACGGCGATGAAGCCGCACT GACATCTGCACTTCAGGGAGTGGAAAAACTACTGCTGATCTCTTCCAGCGAAGTGGGTCAACGTGCCCCGCAGCATCGTAATGTTATTAATGCCGCAAA GGCGGCTGGTGTGAAATTTATCGCTTATACCAGCCTGCTACATGCAGATACCTCCCCGCTCGGCCTCGCCAATGAGCACATCGAGACGGAGAAAATGTT GGCTGATTCTGGCATCGTTTACACCCTGCTGCGCAACGGCTGGTACAGCGAAAACTACCTCGCCAGCGCCCCGGCAGCACTGGAACACGGCGTATTTAT CGGTGCGGCGGGCGATGGCAAAATCGCCTCAGCAACGCGGGCAGATTATGCGGCAGCTGCGGCACGCGTGATTAGCGAAGCCGGTCACGAAGGCAAGGT TTACGAACTGGCGGGCGATAGTGCCTGGACATTGACACAGTTAGCGGCAGAGCTGACCAAACAGAGCGGCAAACAGGTTACCTATCAAAATCTGAGCGA AGCCGATTTCGCCGCGGCACTGAAAAGCGTCGGACTGCCCGACGGACTGGCGGATATGCTGGCGGATTCTGACGTTGGCGCATCGAAAGGTGGTCTGTT TGATAACAGCAAAACGCTTAGCAAATTGATTGGCCGCCCAACGACAACGTTAGCCGAAAGCGTAAGCCATCTTTTTAATGTTAATAACTAGTTAATTAA AGTGGCATCCTCCCGCATCCTCTCTAATAATGACGGGATGCCGGGAGCAATCATGTCTGCTTCCTGAACTTTCTTCTGACAGACCAATGGATGCCAGTA ATGATTAGCGGCGTGCTGTACGCCCTGTTAGCAGGGTTGATGTGGGGGCTTATTTTTGTCGGGCCGTTGATCGTACCGGAATACCCGGCGATGTTGCAG TCGATGGGGCGTTATCTGGCGTTAGGGTTAATTGCGCTGCCCATTGCCTGGCTGGGACGCGTGCGTCTGCGTCAGTTGGCGCGTCGGGACTGGCTTACC GCCTTGATGCTCACTATGATGGGCAACCTCATTTATTACTTCTGCCTTGCCAGTGCCATTCAACGTACTGGCGCGCCTGTTTCCACGATGATTATCGGC ACCCTGCCGGTGGTCATTCCTGTCTTTGCCAATCTGCTTTATAGCCAGCGCGACGGCAAACTCGCGTGGGGAAAACTCGCCCCGGCACTGATTTGTATT GGCATCGGCCTGGCGAGTGTGAATATTGCTGAGTTAAACCACGGACTCCCCGATTTTGACTGGGCACGTTATACCTCTGGCATCGTGCTAGCGTTAGTT TCCGTGGTCTGCTGGGCATGGTATGCCCTGCGCAACGCCCGCTGGCTGCGGGAAAATCCCGACAAACATCCGATGATGTGGGCGACGGCGCAGGCGCTG GTCACGCTGCCGGTTTCTCTCATCGGCTATCTCGTCGCCTGTTACTGGCTGAATATACAAACGCCGGACTTCTCCTTACCTTTTGGCCCCCGTCCGCTG GTGTTTATTAGTCTGATGGTTGCGATAGCCGTGCTTTGCTCATGGGTTGGCGCACTCTGCTGGAACGTCGCCAGCCAGCGATTACCGACAGTGATTCTC GGGCCGCTGATTGTTTTCGAAACGCTGGCAGGTTTGCTGTACACCTTTTTACTCCGCCAGCAAATGCCGCCGCTAATGACGCTGAGCGGTATCGCGCTG TTAGTGATTGGCGTGGTCATTGCGGTCAGAGCAAAACCAGAAAAACCTTTAACTGAATCTGTCTCAGAAAGTTGACACGCTGGCAGTGAGTTAAATAAG CCTCTGCTACGTAAGGGTTATAGCTTTTGCCTTAAAGATGCATTTAAAATACATCTTATCTTATTAAGAATGAGGTATCAGCTATGGCTTATCGCGACC AACCTTTAGGTGAACTGGCGCTCTCTATCCCTCGCGCTTCGGCTCTGTTTCGTAAATATGATATGGATTACTGCTGTGGCGGTAAGCAGACGCTGGCGC GCGCGGCGGCACGTAAAGAACTGGATGTTGAGGTCATTGAAGCTGAACTGGCAAAGCTTGCTGAACAACCGATTGAGAAAGACTGGCGTAGCGCCCCGC TGGCAGAAATCATTGACCATATCATCGTGCGCTAC PŘÍKLAD 1 TTAAGAAATTCCGGTTATCAACTTCGAGGCCCCTTTCAGGTCACCGTTGGCGTATTTCGGGGCGATAGATTTGTATTTGTAGCCGCGAATACTGCGATC GCCCCCGGCGAAAAAACGCAGATCCGGCGGTACTTTGTCGAAATCACCGGTTTCAATCCAGCCCAGCGTGCCGCGTGTAACGAAACGATGGCGATCGTA CAGTGTGCGGATCCAGACGTTCTGCGCCTGGAAAACGGAGAAATCGACATCTGAGCCCCAGGCCGTGTTGGAGTAGTCGATAGAGTAGCGTTGCGAGTC GCCCCTGGTTGGCATCAGGCCACCACGAGAACGCGTGCGGCTAATCATCACCCCAGGATAAAACAGCATCGTGGTGTTGGTAATTTCACCCTGAGTAAA GTGGTCGAGACTCCAGCGCAGGTTAATGGCACGCTGCCAGCCGCTGGAGAGATCCCAGTAGCGAGAAGCCACCAGCGTAGTGGAGTCAGATTCGGTATC GTTCAGGTCAGTGCGCTTAAAACCGCCCTGCACCAAATAATATTGTTCCAGTGGATTCTTCAGCAGCGGCATTTTATAGCTGAAGTCGAGGGTCTGTTC CGGCGCGGAAATACTGGTACTGGTGGTCAGACTGTGACCATAAGAGTTCATCCACGGCTTTTTCCACGTAGCTTTCACGCGCGGTCCCACGTCCGTAGA GTAACCGACCCCGGTTTCGATGGTGTTTTCAGTTCGCGGCGAAACCACGCCCGTCAAGGGTAATACTTTCGTTTCGCGCGCTTTATCAAATTGTGGAGC CACTACGACCGAGTTAAACCAGCCGGTAGCAGAAAGTCGACGGTTAAGTTCCGCCAGATCTTTCGATTCGTACTCATCGCCCTCTTTAAACGGCACCAG ATTTTGCAGGTATTCATCGCGGATTTGTGATCCTTCAAAGGTCACATGCCCAAAGCGGTAACGTTCGCCACTGTTATAATCAATATCCCAGAAGGCTTT ATGCAGGCCGAGCGCAATGCCCAGCTGCGCTTTGGTAAATTCGCTATCGAAATAACCTTTACGCAACGCAATGCTGGTTAAGGACTTTTTGAAATTTTC ATAATCGCCCTGGTTCAGTACCGTGCCAATAGCCGGGCGAGTATCGAGCAATTTCAAATAGTCTTTATCGGTCCGCGCGCCGCCGCGCAATACCACATC GGTGCCGCCAATTAACACCGGCACGCCTGGCGTGACTTTGGCGATCAATACCTGCCGCCCTTTCTTTGGCGGTGGACGGAGATCAAATTCAATGGTCGG CTGGTAATAACCCAGCGCTTTCAGACCTTCGCGGATGGCATCATCGACGCGTGCGCGAAAGCGACGGTCTGGCGTCACTTCATCACTTTCAATCGTAGA AAGCTGCGCACGAACGTTCTTTTCCAGCTGTCCCGATAACCCCTCGACCTGTAGACGGACGTTCGCGGCGACGGCAGATCCGCTTAAGCAGAGTAAGCT TACACAGCATAACTGTCGGATATAGCGCATATTTTCTCCTGAATATCCTTTTTTCCTGCCCCTGGAACGCCGTTAAACGGCTTAACAAAAATCCAGTAA TATGGATTAAAAAGCAGACTAAACCCCAAATATTTCTTATGTTTTACTTTAGACCTATTCACGGTGGTTATTGTGTGCAAATACGCCTCTTGTTACAAC CTTAACCCCAATGACCGATTTTCGGGAGAGCGACACCATGAGTTTATTTGATAAAAAGCATCTGGTTTCCCCCGCCGATGCCCTGCCTGGACGTAACAC CCCGATGCCCGTAGCCACGCTGCATGCGGTCAACGGTCACTCAATGACCAATGTACCTGACGGAATGGAGATTGCCATTTTTGCGATGGGTTGTTTCTG GGGTGTGGAGCGTCTGTTCTGGCAGTTACCCGGCGTTTACAGCACCGCCGCAGGCTATACCGGCGGCTATACGCCAAATCCGACTTATCGGGAAGTGTG CTCCGGTGATACGGGTCATGCCGAAGCGGTACGCATTGTTTACGATCCTTCTGTCATCAGCTATGAGCAGTTGCTACAGGTATTTTGGGAGAATCACGA TCCCGCCCAGGGAATGCGTCAGGGCAATGACCACGGCACGCAGTATCGTTCAGCGATTTATCCGCTGACCCCAGAACAGGATGCCGCAGCTCGCGCCAG TCTGGAACGTTTTCAGGCGGCGATGCTTGCCGCCGATGACGATCGTCACATCACCACGGAAATCGCTAACGCCACACCGTTTTATTATGCCGAAGATGA CCACCAGCAATATCTGCATAAAAACCCGTATGGTTACTGTGGAATTGGCGGAATTGGCGTCTGTCTGCCACCGGAAGCATACCGTTACGGGTACAAATG TAGATTGTTGATAAAGTGCGTTTTGTTTATGCCGAATGCAGCGTGAATGCCTTACCAGGCCTACAAAATCGTCCAAATTCAATATATTGCAGGGACTGC GTAGGCCTGATCGGCATAGCGCATCAGGCAGTATTGCATTTATCAGCAGTCTGAATTTTAACCCTCTGGCGACTTTACAGTACCTTACGCTATACTAGC CACTGAAAATGCCGGATCACTTTCTTCGAATCGGCTTTCAATGTGTATTTCACACAAATTAATCAACTTCCCCTTCCGAGGATCTGGCCTGAAAGGTCG GATAAGATATGTTAAACAGTATTTTAGTCATACTCTGCTTGATCGCTGTAAGTGCGTTCTTCTCGATGTCTGAGATCTCACTTGCCGCCTCACGCAAAA TCAAACTTAAACTGCTGGCTGATGAAGGCAATATAAATGCCCAACGCGTTCTGAATATGCAGGAAAATCCCGGCATGTTCTTTACGGTGGTCCAAATCG GTCTGAACGCAGTTGCGATTCTCGGCGGTATCGTCGGTGATGCGGCATTTTCTCCAGCTTTTCACAGCCTGTTCTCCCGCTATATGTCGGCAGAACTCT CTGAGCAACTGAGCTTTATTCTCTCTTTCTCGTTAGTGACTGGCATGTTTATCCTGTTTGCGGATTTAACCCCGAAACGCATCGGTATGATTGCGCCAG AAGCGGTGGCTTTGCGTATCATCAACCCGATGCGCTTCTGCCTGTACGTTTGCACCCCGCTGGTGTGGTTCTTCAACGGCCTGGCGAACATGATCTTCC GTATTTTCAAACTGCCAATGGTACGTAAAGATGACATCACTTCTGATGACATCTACGCGGTAGTGGAAGCCGGTGCGCTGGCGGGCGTGTTACGTAAAC AGGAACACGAGCTGATTGAAAACGTCTTTGAGCTGGAATCCCGTACCGTTCCGTCTTCAATGACACCGCGTGAAAACGTGATTTGGTTTGATCTCCACG AAGATGAGCAAAGCCTGAAGAATAAGGTGGCGGAACATCCGCACTCTAAGTTCCTCGTCTGTAATGAAGATATTGACCACATCATCGGTTATGTCGATT CTAAAGACCTGCTGAACCGCGTGCTGGCTAACCAAAGCCTGGCACTGAACAGCGGCGTACAAATTCGCAACACGCTGATTGTGCCGGATACGTTAACCC TTTCAGAGGCGTTGGAAAGTTTTAAAACCGCAGGTGAAGACTTCGCGGTGATCATGAACGAGTACGCGCTGGTGGTGGGGATCATCACCCTCAACGACG TGATGACCACGCTGATGGGCGATCTGGTCGGTCAGGGGCTGGAAGAGCAGATTGTCGCCCGTGATGAGAACTCATGGCTGATTGACGGCGGCACCCCAA TTGACGACGTCATGCGCGTGCTGGATATTGACGAGTTCCCGCAGTCGGGCAACTACGAAACCATCGGAGGCTTTATGATGTTTATGCTGCGTAAGATCC CGAAACGCACCGATTCGGTGAAATTCGCCGGCTACAAATTTGAAGTGGTGGATATCGATAACTACCGCATCGACCAGCTGCTGGTGACCCGGATCGACA GCAAGGCCACCGCCCTTTCGCCAAAACTGCCTGACGCTAAAGATAAAGAAGAAAGCGTCGCGTAACCCCCAGAAACATCAACGGCTCCTGAATCAGGAG CCGTCTTATTACTGCATAGCACTTTGGTTAAGCCATCTCTGTTTGCAGACGCATAACCTGACGGTTAACTTCGGACATCACTGACAAATGCAGCTTATC CTTCACTTTTGGGATAAGAATCTTACCCTTATCAAATTCAAAAGCGCCAACGTCCTTGATGTATAACCGTCCACGGAACAGGATCTTTACGTACTTCGC CACCTGAAGTGGGTTGTACCGTTGGAAAATTTTCATTGTTATCTCCTGCTGAGTATTACGCCTGTGCGGTGCCACAATCGGCCCAACTATTATGAGGCG CAAATTTTAATGCCTAGTGACTATAGACTATCCGGGCAATGTTTCCACCGCGTATAACTTTTTTTACCTAAAGGTTACAATTATTCAGAATTATCTTTT TACCGAAGCGCGGTCTTCAGTATAAGCATTCATTTTTCATATGATTTGTGCGCTTGACCGCAAACTGGCATCACACTTGCGGGAAATTCGATAAATAGC ACATATGATTAAAACTCAGACCCAAGTGGTCGGATCACCTGCATATCATAAGAAGGAAACACCATGACCCTACGCAAGATTCTGGCACTCACCTGCCTG CTGTTGCCGATGATGGCTTCCGCACATCAGTTCGAAACCGGTCAGCGAGTGCCGCCGATTGGCATCACCGATCGTGGCGAGTTGGTGCTTGATAAAGAT CAGTTTAGCTACAAAACCTGGAACAGCGCGCAGTTAGTGGGAAAAGTGCGAGTACTGCAACATATTGCTGGTCGCACCTCTGCAAAAGAGAAAAACGCG ACGCTGATTGAAGCGATTAAATCAGCGAAGTTACCGCACGATCGTTACCAGACCACCACCATTGTTAACACCGACGACGCAATTCCGGGTTCCGGCATG TTTGTGCGCAGCAGTCTGGAGAGTAATAAAAAGCTTTATCCCTGGTCGCAGTTTATTGTTGATAGCAATGGCGTCGCACGCGGTGCCTGGCAGCTGGAT GAAAAAAGTTCCGCTGTAGTGGTGCTGGATAAAGACGGTCGCGTGCAATGGGCCAAAGACAGGGCGCTTACTCAGGAAGAGGTGCAGCAAGTGATGGAC CTGCTGCATAAATTAATTAATAAATAGACACTCTGAACCCAGGATTCAGGAACGACTCGCGCGGAGTGTAATCCAGCGGTTTACCCTGCCAGTCGTGAA CGTGCGCTCCGGCAGCTGCGGCCACAGCATGTCCAGCGGCGGTGTCCCAAATATTCGTTGGTCCGAAGCGCGGGTACAGCTGCGCCTGTCCTTCCGCCA CCAGGCAGAATTTCAGCGAAGAGCCGATGGACGTGGTCTGATGTTCGCCAAGCTGTTGCAGATACTCTTTCAGCTCCGCATCTGCATGGGAACGGCTGA TAACCACCAGCGGCGGGCGCGCATCGCGGACCTGAATCTGCTTGCGCACACCGCACTCTTCTTTCCAGGCCTTGCCTTCTGCCGCGCTGTACATTACGT TCATTACCGGCGCATACACCACACCTAAAATCGGTTTGCCATGGTCAATGAGCGCAATGTTAACGGTGAATTCGCCATTGCGTTTAATAAACTCTTTAG TACCATCCAGCGGGTCTACCAGCCAGTAACGCTGCCAGTGCTGACGGACTTCCCAACCGGGAGGATCTTCTTCAGAAAGGACCGGAATATCCGGTGTCA GCGTACGTAAACCGTCCATGATAACGGTGTGAGCGGCAATATCCGCTGCCGTTACCGGAGAATTGTCCGCTTTGCTGACGACGTCCATCGGTTTCGTCC CGTCGTAGACCTGCATAATGGCATCGCCTGCATTCCGTGCAAGCTGGCATACTTGATCTAACATTTCTCCACCTCGTCTCTGTGAGCGGTGTTAACTTA TTGTTTTACTTATACCCTATCGTTAATGAATGCGCCAACTGTGATAGTGTCATCATTTTCAAAGCGTAAAATTGTGGCATTCTTCACTGTTCTATAAGT AAGACGTTTATTCTTCCTTTTCTTTCGTATTCCCGATGATAAAAGGATGTCCCTGATGATTAAGTTTAGCGCAACGCTCCTGGCCACGCTGATTGCCGC CAGTGTGAATGCAGCGACGGTCGATCTGCGTATCATGGAAACCACTGATCTGCATAGCAACATGATGGATTTCGATTATTACAAAGACACCGCCACGGA AAAATTCGGACTGGTACGTACGGCAAGCCTGATTAACGATGCCCGCAATGAAGTGAAAAACAGCGTACTGGTCGATAACGGCGATTTGATTCAGGGGAG TCCGCTGGCCGATTACATGTCGGCGAAAGGATTAAAAGCAGGTGATGTTCATCCGGTTTATAAGGCGCTGAATACGCTGGATTATACGGTCGGTACACT CGGCAATCATGAATTTAACTACGGTCTGGATTACCTGAAAAATGCGCTGGCGGGAGCGAAATTCCCTTATGTAAATGCCAACGTCATTGACGCCAGAAC CAAACAGCCAATGTTTACACCGTATTTAATTAAAGATACCGAAGTGGTCGATAAAGACGGAAAAAAACAGACGCTGAAGATTGGCTATATTGGCGTCGT GCCGCCGCAAATCATGGGCTGGGATAAAGCTAATTTATCCGGAAAAGTGACGGTGAATGATATTACCGAAACCGTGCGCAAATACGTGCCTGAAATGCG CGAGAAAGGTGCCGATGTCGTTGTCGTTCTGGCGCATTCCGGGCTGTCTGCCGATCCGTATAAAGTAATGGCGGAAAACTCAGTTTATTACCTCAGTGA AATTCCGGGCGTTAACGCCATTATGTTTGGTCATGCTCACGCCGTTTTCCCGGGTAAAGATTTTGCTGATATCGAAGGGGCTGATATCACCAAAGGCAC GCTGAATGGTGTTCCGGCGGTAATGCCGGGCATGTGGGGCGATCATCTTGGGGTGGTCGACTTACAACTCAGTAATGACAGCGGTAAATGGCAGGTGAC GCAGGCGAAAGCGGAAGCTCGGCCGATTTACGACATCGCCAATAAAAAATCCCTCGCGGCGGAAGACAGCAAGCTGGTAGAAACACTCAAAGCCGATCA CGATGCCACACGCCAGTTCGTCAGCAAGCCAATCGGTAAATCTGCCGACAATATGTATAGCTATCTGGCGCTGGTGCAGGACGATCCGACCGTGCAAGT AGTGAACAACGCGCAAAAAGCGTATGTCGAACATTACATTCAGGGCGATCCGGATCTGGCAAAACTGCCGGTGCTTTCAGCTGCCGCACCGTTTAAAGT TGGTGGTCGCAAAAATGACCCGGCAAGCTATGTGGAGGTGGAAAAAGGCCAGTTGACCTTCCGTAATGCCGCCGATCTTTATCTCTACCCCAATACGCT GATTGTGGTGAAAGCCAGCGGTAAAGAGGTGAAAGAGTGGCTGGAGTGCTCCGCCGGACAGTTTAACCAGATTGATCCTAACAGCACGAAACCGCAATC ACTCATCAACTGGGATGGTTTCCGCACCTATAACTTTGATGTGATTGATGGTGTGAATTATCAGATTGATGTTACCCAACCTGCCCGTTATGACGGCGA GTGCCAGATGATTAATGCCAATGCGGAAAGGATTAAGAACCTGACCTTTAATGGCAAGCCGATTGATCCGAACGCCATGTTCCTCGTTGCCACCAATAA CTATCGCGCTTACGGCGGCAAATTTGCCGGTACGGGCGACAGCCATATCGCTTTTGCTTCACCGGATGAGAACCGCTCGGTGCTGGCAGCGTGGATTGC TGATGAGTCGAAACGTGCGGGGGAAATTCACCCGGCGGCAGATAACAACTGGCGTTTAGCACCGATAGCTGGCGATAAGAAACTGGATATCCGTTTCGA AACTTCTCCGTCAGATAAAGCCGCAGCGTTTATTAAAGAGAAAGGGCAATATCCGATGAATAAAGTCGCGACCGATGATATCGGGTTTGCGATTTATCA GGTGGATTTGAGTAAGTAAAACACTTCTTTTTCGGCCTATAAATCATCAACCGCATCCGGCATTTATTGGCGGATGCGATGCTGGCGCATCTTATCCGC CCTACAAGCCATGCACCGTAGACCAGATAAGCTCAGCGCATCCGGCAGTTATGCCGCACGTTCATCCCGCACCGCCAACACCTCGGGCAAATTCAACTC AATCCAGTCCGCCAGTGCAGCAACCTTTTCGCTCACCTGCTCGCCCAGCGGCGTGAGGCTATATTCCACATGCGGCGGCACCACCGGATACGCGATACG GTTAAGAAAACCATCCTGTTCTAACGCCTGTAACGACTGCGCAAGCATCTTTTCGCTCACCCCGCCGATTTTGCGCCGCAGGTCGCTAAAGCGATGAGT ACCTTCGCGTAGCGCCACCAGAATCAACACCCCCCAACGGCTGGTGACGTGTTTCAACACCTCGCGCGACGGGCACTGTTCCGCAAAGAGATTACCCTC TTTCAGTTGTTGCGACAGGCTAACCTGACTCATTTCATACTTACCTTTTTGTGCGTACTTACTAAAAGTAAGTTTAGGTGTTAGCGTATTTAAACACAA GACAAAACGATGGAGACTTCCCATGATCGCTATTACTGGTGCCACTGGCCAACTTGGTCACTATGTTATTGAATCCTTGAAGAAAACGGTTCCTGTCAG CCAAATAGTGGCTATCGTTCGTAATCCGGCAAAAGCCCAGGCTCTGGCAGCACAAGGCATTACCGTGCGTCAGGCTGACTACGGCGATGAAGCCGCACT GACATCTGCACTTCAGGGAGTGGAAAAACTACTGCTGATCTCTTCCAGCGAAGTGGGTCAACGTGCCCCGCAGCATCGTAATGTTATTAATGCCGCAAA GGCGGCTGGTGTGAAATTTATCGCTTATACCAGCCTGCTACATGCAGATACCTCCCCGCTCGGCCTCGCCAATGAGCACATCGAGACGGAGAAAATGTT GGCTGATTCTGGCATCGTTTACACCCTGCTGCGCAACGGCTGGTACAGCGAAAACTACCTCGCCAGCGCCCCGGCAGCACTGGAACACGGCGTATTTAT CGGTGCGGCGGGCGATGGCAAAATCGCCTCAGCAACGCGGGCAGATTATGCGGCAGCTGCGGCACGCGTGATTAGCGAAGCCGGTCACGAAGGCAAGGT TTACGAACTGGCGGGCGATAGTGCCTGGACATTGACACAGTTAGCGGCAGAGCTGACCAAACAGAGCGGCAAACAGGTTACCTATCAAAATCTGAGCGA AGCCGATTTCGCCGCGGCACTGAAAAGCGTCGGACTGCCCGACGGACTGGCGGATATGCTGGCGGATTCTGACGTTGGCGCATCGAAAGGTGGTCTGTT TGATAACAGCAAAACGCTTAGCAAATTGATTGGCCGCCCAACGACAACGTTAGCCGAAAGCGTAAGCCATCTTTTTAATGTTAATAACTAGTTAATTAA AGTGGCATCCTCCCGCATCCTCTCTAATAATGACGGGATGCCGGGAGCAATCATGTCTGCTTCCTGAACTTTCTTCTGACAGACCAATGGATGCCAGTA ATGATTAGCGGCGTGCTGTACGCCCTGTTAGCAGGGTTGATGTGGGGGCTTATTTTTGTCGGGCCGTTGATCGTACCGGAATACCCGGCGATGTTGCAG TCGATGGGGCGTTATCTGGCGTTAGGGTTAATTGCGCTGCCCATTGCCTGGCTGGGACGCGTGCGTCTGCGTCAGTTGGCGCGTCGGGACTGGCTTACC GCCTTGATGCTCACTATGATGGGCAACCTCATTTATTACTTCTGCCTTGCCAGTGCCATTCAACGTACTGGCGCGCCTGTTTCCACGATGATTATCGGC ACCCTGCCGGTGGTCATTCCTGTCTTTGCCAATCTGCTTTATAGCCAGCGCGACGGCAAACTCGCGTGGGGAAAACTCGCCCCGGCACTGATTTGTATT GGCATCGGCCTGGCGAGTGTGAATATTGCTGAGTTAAACCACGGACTCCCCGATTTTGACTGGGCACGTTATACCTCTGGCATCGTGCTAGCGTTAGTT TCCGTGGTCTGCTGGGCATGGTATGCCCTGCGCAACGCCCGCTGGCTGCGGGAAAATCCCGACAAACATCCGATGATGTGGGCGACGGCGCAGGCGCTG GTCACGCTGCCGGTTTCTCTCATCGGCTATCTCGTCGCCTGTTACTGGCTGAATATACAAACGCCGGACTTCTCCTTACCTTTTGGCCCCCGTCCGCTG GTGTTTATTAGTCTGATGGTTGCGATAGCCGTGCTTTGCTCATGGGTTGGCGCACTCTGCTGGAACGTCGCCAGCCAGCGATTACCGACAGTGATTCTC GGGCCGCTGATTGTTTTCGAAACGCTGGCAGGTTTGCTGTACACCTTTTTACTCCGCCAGCAAATGCCGCCGCTAATGACGCTGAGCGGTATCGCGCTG TTAGTGATTGGCGTGGTCATTGCGGTCAGAGCAAAACCAGAAAAACCTTTAACTGAATCTGTCTCAGAAAGTTGACACGCTGGCAGTGAGTTAAATAAG CCTCTGCTACGTAAGGGTTATAGCTTTTGCCTTAAAGATGCATTTAAAATACATCTTATCTTATTAAGAATGAGGTATCAGCTATGGCTTATCGCGACC AACCTTTAGGTGAACTGGCGCTCTCTATCCCTCGCGCTTCGGCTCTGTTTCGTAAATATGATATGGATTACTGCTGTGGCGGTAAGCAGACGCTGGCGC GCGCGGCGGCACGTAAAGAACTGGATGTTGAGGTCATTGAAGCTGAACTGGCAAAGCTTGCTGAACAACCGATTGAGAAAGACTGGCGTAGCGCCCCGC TGGCAGAAATCATTGACCATATCATCGTGCGCTAC PŘÍKLAD 2 TTAAGAAATTCCGGTTATCAACTTCGAGGCCCCTTTCAGGTCACCGTTGGCGTATTTCGGGGCGATAGATTTGTATTTGTAGCCGCGAATACTGCGATC GCCCCCGGCGAAAAAACGCAGATCCGGCGGTACTTTGTCGAAATCACCGGTTTCAATCCAGCCCAGCGTGCCGCGTGTAACGAAACGATGGCGATCGTA CAGTGTGCGGATCCAGACGTTCTGCGCCTGGAAAACGGAGAAATCGACATCTGAGCCCCAGGCCGTGTTGGAGTAGTCGATAGAGTAGCGTTGCGAGTC GCCCCTGGTTGGCATCAGGCCACCACGAGAACGCGTGCGGCTAATCATCACCCCAGGATAAAACAGCATCGTGGTGTTGGTAATTTCACCCTGAGTAAA GTGGTCGAGACTCCAGCGCAGGTTAATGGCACGCTGCCAGCCGCTGGAGAGATCCCAGTAGCGAGAAGCCACCAGCGTAGTGGAGTCAGATTCGGTATC GTTCAGGTCAGTGCGCTTAAAACCGCCCTGCACCAAATAATATTGTTCCAGTGGATTCTTCAGCAGCGGCATTTTATAGCTGAAGTCGAGGGTCTGTTC CGGCGCGGAAATACTGGTACTGGTGGTCAGACTGTGACCATAAGAGTTCATCCACGGCTTTTTCCACGTAGCTTTCACGCGCGGTCCCACGTCCGTAGA GTAACCGACCCCGGTTTCGATGGTGTTTTCAGTTCGCGGCGAAACCACGCCCGTCAAGGGTAATACTTTCGTTTCGCGCGCTTTATCAAATTGTGGAGC CACTACGACCGAGTTAAACCAGCCGGTAGCAGAAAGTCGACGGTTAAGTTCCGCCAGATCTTTCGATTCGTACTCATCGCCCTCTTTAAACGGCACCAG ATTTTGCAGGTATTCATCGCGGATTTGTGATCCTTCAAAGGTCACATGCCCAAAGCGGTAACGTTCGCCACTGTTATAATCAATATCCCAGAAGGCTTT ATGCAGGCCGAGCGCAATGCCCAGCTGCGCTTTGGTAAATTCGCTATCGAAATAACCTTTACGCAACGCAATGCTGGTTAAGGACTTTTTGAAATTTTC ATAATCGCCCTGGTTCAGTACCGTGCCAATAGCCGGGCGAGTATCGAGCAATTTCAAATAGTCTTTATCGGTCCGCGCGCCGCCGCGCAATACCACATC GGTGCCGCCAATTAACACCGGCACGCCTGGCGTGACTTTGGCGATCAATACCTGCCGCCCTTTCTTTGGCGGTGGACGGAGATCAAATTCAATGGTCGG CTGGTAATAACCCAGCGCTTTCAGACCTTCGCGGATGGCATCATCGACGCGTGCGCGAAAGCGACGGTCTGGCGTCACTTCATCACTTTCAATCGTAGA AAGCTGCGCACGAACGTTCTTTTCCAGCTGTCCCGATAACCCCTCGACCTGTAGACGGACGTTCGCGGCGACGGCAGATCCGCTTAAGCAGAGTAAGCT TACACAGCATAACTGTCGGATATAGCGCATATTTTCTCCTGAATATCCTTTTTTCCTGCCCCTGGAACGCCGTTAAACGGCTTAACAAAAATCCAGTAA TATGGATTAAAAAGCAGACTAAACCCCAAATATTTCTTATGTTTTACTTTAGACCTATTCACGGTGGTTATTGTGTGCAAATACGCCTCTTGTTACAAC CTTAACCCCAATGACCGATTTTCGGGAGAGCGACACCATGAGTTTATTTGATAAAAAGCATCTGGTTTCCCCCGCCGATGCCCTGCCTGGACGTAACAC CCCGATGCCCGTAGCCACGCTGCATGCGGTCAACGGTCACTCAATGACCAATGTACCTGACGGAATGGAGATTGCCATTTTTGCGATGGGTTGTTTCTG GGGTGTGGAGCGTCTGTTCTGGCAGTTACCCGGCGTTTACAGCACCGCCGCAGGCTATACCGGCGGCTATACGCCAAATCCGACTTATCGGGAAGTGTG CTCCGGTGATACGGGTCATGCCGAAGCGGTACGCATTGTTTACGATCCTTCTGTCATCAGCTATGAGCAGTTGCTACAGGTATTTTGGGAGAATCACGA TCCCGCCCAGGGAATGCGTCAGGGCAATGACCACGGCACGCAGTATCGTTCAGCGATTTATCCGCTGACCCCAGAACAGGATGCCGCAGCTCGCGCCAG TCTGGAACGTTTTCAGGCGGCGATGCTTGCCGCCGATGACGATCGTCACATCACCACGGAAATCGCTAACGCCACACCGTTTTATTATGCCGAAGATGA CCACCAGCAATATCTGCATAAAAACCCGTATGGTTACTGTGGAATTGGCGGAATTGGCGTCTGTCTGCCACCGGAAGCATAGCGTTACGGGTACAAATG TAGATTGTTGATAAAGTGCGTTTTGTTTATGCCGAATGCAGCGTGAATGCCTTACCAGGCCTACAAAATCGTCCAAATTCAATATATTGCAGGGACTGC GTAGGCCTGATCGGCATAGCGCATCAGGCAGTATTGCATTTATCAGCAGTCTGAATTTTAACCCTCTGGCGACTTTACAGTACCTTACGCTATACTAGC CACTGAAAATGCCGGATCACTTTCTTCGAATCGGCTTTCAATGTGTATTTCACACAAATTAATCAACTTCCCCTTCCGAGGATCTGGCCTGAAAGGTCG GATAAGATATGTTAAACAGTATTTTAGTCATACTCTGCTTGATCGCTGTAAGTGCGTTCTTCTCGATGTCTGAGATCTCACTTGCCGCCTCACGCAAAA TCAAACTTAAACTGCTGGCTGATGAAGGCAATATAAATGCCCAACGCGTTCTGAATATGCAGGAAAATCCCGGCATGTTCTTTACGGTGGTCCAAATCG GTCTGAACGCAGTTGCGATTCTCGGCGGTATCGTCGGTGATGCGGCATTTTCTCCAGCTTTTCACAGCCTGTTCTCCCGCTATATGTCGGCAGAACTCT CTGAGCAACTGAGCTTTATTCTCTCTTTCTCGTTAGTGACTGGCATGTTTATCCTGTTTGCGGATTTAACCCCGAAACGCATCGGTATGATTGCGCCAG AAGCGGTGGCTTTGCGTATCATCAACCCGATGCGCTTCTGCCTGTACGTTTGCACCCCGCTGGTGTGGTTCTTCAACGGCCTGGCGAACATGATCTTCC GTATTTTCAAACTGCCAATGGTACGTAAAGATGACATCACTTCTGATGACATCTACGCGGTAGTGGAAGCCGGTGCGCTGGCGGGCGTGTTACGTAAAC AGGAACACGAGCTGATTGAAAACGTCTTTGAGCTGGAATCCCGTACCGTTCCGTCTTCAATGACACCGCGTGAAAACGTGATTTGGTTTGATCTCCACG AAGATGAGCAAAGCCTGAAGAATAAGGTGGCGGAACATCCGCACTCTAAGTTCCTCGTCTGTAATGAAGATATTGACCACATCATCGGTTATGTCGATT CTAAAGACCTGCTGAACCGCGTGCTGGCTAACCAAAGCCTGGCACTGAACAGCGGCGTACAAATTCGCAACACGCTGATTGTGCCGGATACGTTAACCC TTTCAGAGGCGTTGGAAAGTTTTAAAACCGCAGGTGAAGACTTCGCGGTGATCATGAACGAGTACGCGCTGGTGGTGGGGATCATCACCCTCAACGACG TGATGACCACGCTGATGGGCGATCTGGTCGGTCAGGGGCTGGAAGAGCAGATTGTCGCCCGTGATGAGAACTCATGGCTGATTGACGGCGGCACCCCAA TTGACGACGTCATGCGCGTGCTGGATATTGACGAGTTCCCGCAGTCGGGCAACTACGAAACCATCGGAGGCTTTATGATGTTTATGCTGCGTAAGATCC CGAAACGCACCGATTCGGTGAAATTCGCCGGCTACAAATTTGAAGTGGTGGATATCGATAACTACCGCATCGACCAGCTGCTGGTGACCCGGATCGACA GCAAGGCCACCGCCCTTTCGCCAAAACTGCCTGACGCTAAAGATAAAGAAGAAAGCGTCGCGTAACCCCCAGAAACATCAACGGCTCCTGAATCAGGAG CCGTCTTATTACTGCATAGCACTTTGGTTAAGCCATCTCTGTTTGCAGACGCATAACCTGACGGTTAACTTCGGACATCACTGACAAATGCAGCTTATC CTTCACTTTTGGGATAAGAATCTTACCCTTATCAAATTCAAAAGCGCCAACGTCCTTGATGTATAACCGTCCACGGAACAGGATCTTTACGTACTTCGC CACCTGAAGTGGGTTGTACCGTTGGAAAATTTTCATTGTTATCTCCTGCTGAGTATTACGCCTGTGCGGTGCCACAATCGGCCCAACTATTATGAGGCG CAAATTTTAATGCCTAGTGACTATAGACTATCCGGGCAATGTTTCCACCGCGTATAACTTTTTTTACCTAAAGGTTACAATTATTCAGAATTATCTTTT TACCGAAGCGCGGTCTTCAGTATAAGCATTCATTTTTCATATGATTTGTGCGCTTGACCGCAAACTGGCATCACACTTGCGGGAAATTCGATAAATAGC ACATATGATTAAAACTCAGACCCAAGTGGTCGGATCACCTGCATATCATAAGAAGGAAACACCATGACCCTACGCAAGATTCTGGCACTCACCTGCCTG CTGTTGCCGATGATGGCTTCCGCACATCAGTTCGAAACCGGTCAGCGAGTGCCGCCGATTGGCATCACCGATCGTGGCGAGTTGGTGCTTGATAAAGAT CAGTTTAGCTACAAAACCTGGAACAGCGCGCAGTTAGTGGGAAAAGTGCGAGTACTGCAACATATTGCTGGTCGCACCTCTGCAAAAGAGAAAAACGCG ACGCTGATTGAAGCGATTAAATCAGCGAAGTTACCGCACGATCGTTACCAGACCACCACCATTGTTAACACCGACGACGCAATTCCGGGTTCCGGCATG TTTGTGCGCAGCAGTCTGGAGAGTAATAAAAAGCTTTATCCCTGGTCGCAGTTTATTGTTGATAGCAATGGCGTCGCACGCGGTGCCTGGCAGCTGGAT GAAAAAAGTTCCGCTGTAGTGGTGCTGGATAAAGACGGTCGCGTGCAATGGGCCAAAGACAGGGCGCTTACTCAGGAAGAGGTGCAGCAAGTGATGGAC CTGCTGCATAAATTAATTAATAAATAGACACTCTGAACCCAGGATTCAGGAACGACTCGCGCGGAGTGTAATCCAGCGGTTTACCCTGCCAGTCGTGAA CGTGCGCTCCGGCAGCTGCGGCCACAGCATGTCCAGCGGCGGTGTCCCAAATATTCGTTGGTCCGAAGCGCGGGTACAGCTGCGCCTGTCCTTCCGCCA CCAGGCAGAATTTCAGCGAAGAGCCGATGGACGTGGTCTGATGTTCGCCAAGCTGTTGCAGATACTCTTTCAGCTCCGCATCTGCATGGGAACGGCTGA TAACCACCAGCGGCGGGCGCGCATCGCGGACCTGAATCTGCTTGCGCACACCGCACTCTTCTTTCCAGGCCTTGCCTTCTGCCGCGCTGTACATTACGT TCATTACCGGCGCATACACCACACCTAAAATCGGTTTGCCATGGTCAATGAGCGCAATGTTAACGGTGAATTCGCCATTGCGTTTAATAAACTCTTTAG TACCATCCAGCGGGTCTACCAGCCAGTAACGCTGCCAGTGCTGACGGACTTCCCAACCGGGAGGATCTTCTTCAGAAAGGACCGGAATATCCGGTGTCA GCGTACGTAAACCGTCCATGATAACGGTGTGAGCGGCAATATCCGCTGCCGTTACCGGAGAATTGTCCGCTTTGCTGACGACGTCCATCGGTTTCGTCC CGTCGTAGACCTGCATAATGGCATCGCCTGCATTCCGTGCAAGCTGGCATACTTGATCTAACATTTCTCCACCTCGTCTCTGTGAGCGGTGTTAACTTA TTGTTTTACTTATACCCTATCGTTAATGAATGCGCCAACTGTGATAGTGTCATCATTTTCAAAGCGTAAAATTGTGGCATTCTTCACTGTTCTATAAGT AAGACGTTTATTCTTCCTTTTCTTTCGTATTCCCGATGATAAAAGGATGTCCCTGATGATTAAGTTTAGCGCAACGCTCCTGGCCACGCTGATTGCCGC CAGTGTGAATGCAGCGACGGTCGATCTGCGTATCATGGAAACCACTGATCTGCATAGCAACATGATGGATTTCGATTATTACAAAGACACCGCCACGGA AAAATTCGGACTGGTACGTACGGCAAGCCTGATTAACGATGCCCGCAATGAAGTGAAAAACAGCGTACTGGTCGATAACGGCGATTTGATTCAGGGGAG TCCGCTGGCCGATTACATGTCGGCGAAAGGATTAAAAGCAGGTGATGTTCATCCGGTTTATAAGGCGCTGAATACGCTGGATTATACGGTCGGTACACT CGGCAATCATGAATTTAACTACGGTCTGGATTACCTGAAAAATGCGCTGGCGGGAGCGAAATTCCCTTATGTAAATGCCAACGTCATTGACGCCAGAAC CAAACAGCCAATGTTTACACCGTATTTAATTAAAGATACCGAAGTGGTCGATAAAGACGGAAAAAAACAGACGCTGAAGATTGGCTATATTGGCGTCGT GCCGCCGCAAATCATGGGCTGGGATAAAGCTAATTTATCCGGAAAAGTGACGGTGAATGATATTACCGAAACCGTGCGCAAATACGTGCCTGAAATGCG CGAGAAAGGTGCCGATGTCGTTGTCGTTCTGGCGCATTCCGGGCTGTCTGCCGATCCGTATAAAGTAATGGCGGAAAACTCAGTTTATTACCTCAGTGA AATTCCGGGCGTTAACGCCATTATGTTTGGTCATGCTCACGCCGTTTTCCCGGGTAAAGATTTTGCTGATATCGAAGGGGCTGATATCACCAAAGGCAC GCTGAATGGTGTTCCGGCGGTAATGCCGGGCATGTGGGGCGATCATCTTGGGGTGGTCGACTTACAACTCAGTAATGACAGCGGTAAATGGCAGGTGAC GCAGGCGAAAGCGGAAGCTCGGCCGATTTACGACATCGCCAATAAAAAATCCCTCGCGGCGGAAGACAGCAAGCTGGTAGAAACACTCAAAGCCGATCA CGATGCCACACGCCAGTTCGTCAGCAAGCCAATCGGTAAATCTGCCGACAATATGTATAGCTATCTGGCGCTGGTGCAGGACGATCCGACCGTGCAAGT AGTGAACAACGCGCAAAAAGCGTATGTCGAACATTACATTCAGGGCGATCCGGATCTGGCAAAACTGCCGGTGCTTTCAGCTGCCGCACCGTTTAAAGT TGGTGGTCGCAAAAATGACCCGGCAAGCTATGTGGAGGTGGAAAAAGGCCAGTTGACCTTCCGTAATGCCGCCGATCTTTATCTCTACCCCAATACGCT GATTGTGGTGAAAGCCAGCGGTAAAGAGGTGAAAGAGTGGCTGGAGTGCTCCGCCGGACAGTTTAACCAGATTGATCCTAACAGCACGAAACCGCAATC ACTCATCAACTGGGATGGTTTCCGCACCTATAACTTTGATGTGATTGATGGTGTGAATTATCAGATTGATGTTACCCAACCTGCCCGTTATGACGGCGA GTGCCAGATGATTAATGCCAATGCGGAAAGGATTAAGAACCTGACCTTTAATGGCAAGCCGATTGATCCGAACGCCATGTTCCTCGTTGCCACCAATAA CTATCGCGCTTACGGCGGCAAATTTGCCGGTACGGGCGACAGCCATATCGCTTTTGCTTCACCGGATGAGAACCGCTCGGTGCTGGCAGCGTGGATTGC TGATGAGTCGAAACGTGCGGGGGAAATTCACCCGGCGGCAGATAACAACTGGCGTTTAGCACCGATAGCTGGCGATAAGAAACTGGATATCCGTTTCGA AACTTCTCCGTCAGATAAAGCCGCAGCGTTTATTAAAGAGAAAGGGCAATATCCGATGAATAAAGTCGCGACCGATGATATCGGGTTTGCGATTTATCA GGTGGATTTGAGTAAGTAAAACACTTCTTTTTCGGCCTATAAATCATCAACCGCATCCGGCATTTATTGGCGGATGCGATGCTGGCGCATCTTATCCGC CCTACAAGCCATGCACCGTAGACCAGATAAGCTCAGCGCATCCGGCAGTTATGCCGCACGTTCATCCCGCACCGCCAACACCTCGGGCAAATTCAACTC AATCCAGTCCGCCAGTGCAGCAACCTTTTCGCTCACCTGCTCGCCCAGCGGCGTGAGGCTATATTCCACATGCGGCGGCACCACCGGATACGCGATACG GTTAAGAAAACCATCCTGTTCTAACGCCTGTAACGACTGCGCAAGCATCTTTTCGCTCACCCCGCCGATTTTGCGCCGCAGGTCGCTAAAGCGATGAGT ACCTTCGCGTAGCGCCACCAGAATCAACACCCCCCAACGGCTGGTGACGTGTTTCAACACCTCGCGCGACGGGCACTGTTCCGCAAAGAGATTACCCTC TTTCAGTTGTTGCGACAGGCTAACCTGACTCATTTCATACTTACCTTTTTGTGCGTACTTACTAAAAGTAAGTTTAGGTGTTAGCGTATTTAAACACAA GACAAAACGATGGAGACTTCCCATGATCGCTATTACTGGTGCCACTGGCCAACTTGGTCACTATGTTATTGAATCCTTGAAGAAAACGGTTCCTGTCAG CCAAATAGTGGCTATCGTTCGTAATCCGGCAAAAGCCCAGGCTCTGGCAGCACAAGGCATTACCGTGCGTCAGGCTGACTACGGCGATGAAGCCGCACT GACATCTGCACTTCAGGGAGTGGAAAAACTACTGCTGATCTCTTCCAGCGAAGTGGGTCAACGTGCCCCGCAGCATCGTAATGTTATTAATGCCGCAAA GGCGGCTGGTGTGAAATTTATCGCTTATACCAGCCTGCTACATGCAGATACCTCCCCGCTCGGCCTCGCCAATGAGCACATCGAGACGGAGAAAATGTT GGCTGATTCTGGCATCGTTTACACCCTGCTGCGCAACGGCTGGTACAGCGAAAACTACCTCGCCAGCGCCCCGGCAGCACTGGAACACGGCGTATTTAT CGGTGCGGCGGGCGATGGCAAAATCGCCTCAGCAACGCGGGCAGATTATGCGGCAGCTGCGGCACGCGTGATTAGCGAAGCCGGTCACGAAGGCAAGGT TTACGAACTGGCGGGCGATAGTGCCTGGACATTGACACAGTTAGCGGCAGAGCTGACCAAACAGAGCGGCAAACAGGTTACCTATCAAAATCTGAGCGA AGCCGATTTCGCCGCGGCACTGAAAAGCGTCGGACTGCCCGACGGACTGGCGGATATGCTGGCGGATTCTGACGTTGGCGCATCGAAAGGTGGTCTGTT TGATAACAGCAAAACGCTTAGCAAATTGATTGGCCGCCCAACGACAACGTTAGCCGAAAGCGTAAGCCATCTTTTTAATGTTAATAACTAGTTAATTAA AGTGGCATCCTCCCGCATCCTCTCTAATAATGACGGGATGCCGGGAGCAATCATGTCTGCTTCCTGAACTTTCTTCTGACAGACCAATGGATGCCAGTA ATAATTAGCGGCGTGCTGTACGCCCTGTTAGCAGGGTTGATGTGGGGGCTTATTTTTGTCGGGCCGTTGATCGTACCGGAATACCCGGCGATGTTGCAG TCGATGGGGCGTTATCTGGCGTTAGGGTTAATTGCGCTGCCCATTGCCTGGCTGGGACGCGTGCGTCTGCGTCAGTTGGCGCGTCGGGACTGGCTTACC GCCTTGATGCTCACTATGATGGGCAACCTCATTTATTACTTCTGCCTTGCCAGTGCCATTCAACGTACTGGCGCGCCTGTTTCCACGATGATTATCGGC ACCCTGCCGGTGGTCATTCCTGTCTTTGCCAATCTGCTTTATAGCCAGCGCGACGGCAAACTCGCGTGGGGAAAACTCGCCCCGGCACTGATTTGTATT GGCATCGGCCTGGCGAGTGTGAATATTGCTGAGTTAAACCACGGACTCCCCGATTTTGACTGGGCACGTTATACCTCTGGCATCGTGCTAGCGTTAGTT TCCGTGGTCTGCTGGGCATGGTATGCCCTGCGCAACGCCCGCTGGCTGCGGGAAAATCCCGACAAACATCCGATGATGTGGGCGACGGCGCAGGCGCTG GTCACGCTGCCGGTTTCTCTCATCGGCTATCTCGTCGCCTGTTACTGGCTGAATATACAAACGCCGGACTTCTCCTTACCTTTTGGCCCCCGTCCGCTG GTGTTTATTAGTCTGATGGTTGCGATAGCCGTGCTTTGCTCATGGGTTGGCGCACTCTGCTGGAACGTCGCCAGCCAGCGATTACCGACAGTGATTCTC GGGCCGCTGATTGTTTTCGAAACGCTGGCAGGTTTGCTGTACACCTTTTTACTCCGCCAGCAAATGCCGCCGCTAATGACGCTGAGCGGTATCGCGCTG TTAGTGATTGGCGTGGTCATTGCGGTCAGAGCAAAACCAGAAAAACCTTTAACTGAATCTGTCTCAGAAAGTTGACACGCTGGCAGTGAGTTAAATAAG CCTCTGCTACGTAAGGGTTATAGCTTTTGCCTTAAAGATGCATTTAAAATACATCTTATCTTATTAAGAATGAGGTATCAGCTATGGCTTATCGCGACC AACCTTTAGGTGAACTGGCGCTCTCTATCCCTCGCGCTTCGGCTCTGTTTCGTAAATATGATATGGATTACTGCTGTGGCGGTAAGCAGACGCTGGCGC GCGCGGCGGCACGTAAAGAACTGGATGTTGAGGTCATTGAAGCTGAACTGGCAAAGCTTGCTGAACAACCGATTGAGAAAGACTGGCGTAGCGCCCCGC TGGCAGAAATCATTGACCATATCATCGTGCGCTAC PREDIKCE GENŮ U EUKARYOT Genomy jednobuněčných eukaryot se navzájem výrazně liší (frekvence intronů, jak velká část genomu je tvořená geny kódujícími proteiny). Pro některá jednobuněčná eukaryota (s nízkou frekvencí intronů) je možné použít stejné postupy jako pro prokaryota. ÚKOL 2 Charakterizujte část genomu Candida albicans (kvasinka) pomocí programu GeneMark (http://exon.gatech.edu/GeneMark), verze pro nižší eukaryota. U identifikovaných genů určete možnou funkci. TCAATGTCTTTGTGATTAATCTTCGGACATATGCAATTTTGAAATATTAATTATGTTCATATCAACATCACGAGCTTGGAACTGAATATTGAGTAATCA CGATATTGCACCGACTCAACCTTGATTAAATCTGCTAGTGTACACTAATTGTGACCTTTCTATTAGATCATTATCTTTCTTAAATGTGTTGAAATCACG AGAAATTGTAAATACTACTAAGCCTGTATATTCAACACAGAAATACATCATAGATGTTTTTTTTTCTTATTATTGTGTGTCAATTAACCTGTCTCCCTC TCCTTTGAACAATCAATTAGTACTTTACAACTCACAATTTGTAAACCCTGTCCGGATACACATTTTATTTCAAATTACCGTGAGTGCATGTACTAAATT CAACTGGTCGTCGTTTCATTTCAAGGTCAACGCATAGTCTAATAAGTATGTCGTTTCGAGAACTCTACTGTCAATCTCTCGTGTAAAGAAGTATTTTTT TTTTGGTCCAGTTTTGCAACAAATTATACTTTAAATTCTCATAGAATGTTTATTTTCATTTACTACAGACCTTACAAAATCCTAACCAGACTAGATGTC TATCTATGGAGCATCAGTACATTCCCAATCTGTTGGTTCATAAGCATCAGGTTGATCAATAGCCTTAATTTCTTCATTAGTCAATTCAAAATCATCAAC AGAAAGATTTTCCTTTAATCGAGATGGAGTTTTTGTTTTTGGTAATGGTATATAACCTTTTTGTAATGACCATTTAATCAATATTTGAGCAGCTGATTT ATTATACTTTTGCATAATTTCTTGAAATTCAGTATTGTTGACTTGTAATTTGTTACCATGGGTTAATGGTGCATATGCCTCAACATTGATACCTTTACT TAAACACCAAGTAGCTAAATCCTGTCTCATACACCAAGGACTAATTTCAATTTGATTGACAGCTGGAGGGATCGTTGCATTGGTCAACAATTCTTCAAT ATGATGTTTACCATAATTAGAAACCCCAATGTTTTTAATCCATCCTTTTTCCACAGCATCCTGCAAAACTTTCCAGCTTTCTAAACGTTTGGTCTTACC TGGTAATGGAGAATGAATCAATAATAAATCAATATATTCTAATTTATCACCAACTTGAGCCATCATTGTTGAAATGGCTTGTTTAGTGCTTGAAGTACC CAATTGATTATTCCAAAGCTTTGTGGTGTAAAAAAACTCAGATCGTGGTATATTGGGGTTCTCTCGTAAGAATTTACTTATACCTTCAATGACTTCTTC TTCGTTTCCATATAACACTGCAGTATCAAAATGACGATATCCAACTTTACAAGCTTCATAAACTACCGAAACCGTTTTATTTCTTGGGATATCATAACA TCCTAATCCAATTGATGGAATGGTATGACCGGAATTGAGTTTGATTAATCGATATGACATTTTTTCTTAAGCAAATTGAGCAATTGAGTGAAATATAAA GAGTATTTGAAATCTAGAGAATTAAAAAAAAAGAAGTGGTTGTTGTAAGAACAATTGACCAATTTGGGTGGAACTCCTTTTTTATTGGTTTCGAACTGT GGGGATGGCAAAGCAAAGCAATCTTTTGTACAGTCTCAAAACTTCTTTCTATTATCGTTCCTTCGTGTAAAATCTGCACATTTGCATTGCCCAATTTTG TTTATTATATCATTTCGTTACAATGAATTGACAGACGAGTATTTGGTTATAGTATTTGGACTATTTGGTCTTGTAGATCACCATTTACACTTATAATCA AAATTTCTCTGGAATTAAGATCTAACTTTGAAAACAATACAGACGATTCCAGCCTGTTATTCCCGGGATTGGATCTTTTGGTTGCGTTAGTCCGAGGAT ATATGCACGTGAATTGATGGTGAAGCTATAAGACCAGTGGTAGTGTACTGAGACGAAAAATGGAATAGAGCAAGAAAGAAGTGTGCTGAGGGTTGAGAA AAATAATAGATACTGTAGTGGTAGTAGTAGTGGTATGATGCAAATTTATTGTTTGTATTTTTTTTTTTGTTGCTCCTCGTCGACAACGACTGCTGATTC ACACCACCACCACCTTTGTAGTCAAATTAAAATCGTCGGTGGCAAAAAAAAACACAGACACACACATACAGACAATTGCGATATTTTATTTTAGGGTCA ATTTGGAACGTCACCATACAGTATTCAGTATGATTAATTTGACTTGCAAAATACAAATCGCGTGGTTCAATTCCGAAAAATTGATAGTGTTCCATGAAA AATTACAAGGAGAGCCGTGAATTATAACAAATTTACTCACATTTTGGCTGGCAATTTCTTTAACCCCCTTAATTTTTTTCATGCAGTTTATTATTTTAC GTAGAAGCTTATTCTCTAAACACTCGGTTATCCGAATATATTTTGCTTTCAGAGCAATTGACTGATATAATACCATCAATTGAATAAATCAGTATCATC AAATGCTAGTTTGAGTTCAATAAAAGGTATAATCGGTGTTAACTGAAAGGTATTGTCTAATTAATTAGGTTGCGGTTTTTGGATCAGCTGTTTCAAATC CAGGCTCGTGGTGCACATTTTTTATTTTTGTTGTGATGATTTTTTCTTTTCTTTGCTTAATAGTTAGTTAGTAAAATGGATAATGACGCAAAATTGCTT CTGAATGGATGATAATGCCCCGGGTATGGCGTGTCCCGTACAAATCATATTATGCAAAAATAGCCAAAATAGACAAAAATAGGTGGAAATTGTTTCAAC CAACCCACTCAACCACTAAAATTTGTCTTGTCAAAGCCAATTCCAACGAAAATTGGATTTTTTTTGACTTAATATATATTAACCCTTGAAATTCCCTTC AATTGGAAAATTTTTTTAATTTATTTATTTCTTCTTTCTTTTTTTCTTTTTCTTTTTCTTTCTTTTATTCATCACAATTGAATTCAAATCAATTAACAT CAACAAACTTTACAATCAAATTAATTAACAATGGCTATTAAAATTGGTATTAACGGTTTCGGTAGAATCGGTAGATTAGTCTTAAGAGTTGCTTTGGGC AGAAAAGACATTGAAGTTGTCGCCGTCAACGATCCATTCATTGCTCCAGACTATGCTGCTTACATGTTCAAATACGATTCTACTCACGGTAGATACAAG GGTGAAGTCACTGCTTCTGGTGACGACTTGGTCATTGATGGTCACAAGATTAAAGTTTTCCAAGAAAGAGACCCAGCTAACATTCCATGGGGTAAATCT GGTGTTGACTACGTTATTGAATCCACCGGTGTTTTCACCAAACTCGAAGGTGCTCAAAAACACATTGATGCTGGTGCCAAAAAAGTTATCATCACTGCT CCATCTGCTGATGCCCCAATGTTTGTTGTCGGTGTTAACGAAGACAAATACACTCCAGACTTGAAGATTATCTCCAATGCTTCTTGTACCACCAACTGT TTGGCTCCATTAGCTAAAGTCGTCAACGATACTTTCGGTATTGAAGAAGGTTTGATGACCACTGTCCACTCCATCACTGCTACCCAAAAGACCGTTGAC GGTCCATCCCACAAGGACTGGAGAGGTGGTAGAACTGCTTCTGGTAACATTATCCCATCTTCCACTGGTGCTGCTAAAGCCGTTGGTAAGGTTATTCCA GAATTGAACGGTAAATTGACTGGTATGTCTTTGAGAGTCCCAACCACCGATGTTTCCGTTGTTGACTTGACTGTCAGATTGAAGAAAGCTGCTTCTTAC GAAGAAATTGCTCAAGCTATCAAGAAAGCTTCTGAAGGTCCATTGAAAGGTGTTTTGGGCTACACTGAAGATGCTGTTGTCTCCACCGATTTCTTGGGT TCAAGCTACTCATCTATCTTTGATGAAAAAGCCGGTATCTTGTTGTCCCCAACTTTCGTCAAATTGATTTCCTGGTACGATAACGAATACGGTTACTCC ACCAGAGTTGTTGACTTGTTGGAACACGTTGCTAAAGCTTCTGCTTGAAGTAAAACCAGACTTTGATTTGATTTTTTTAGTTAATTGAAGTTATATAAT GAGTTTTTGAAATAAATATCTTACATTGAAGAGAATATTGATGTGTTTTTTTTTTGTTTTTATATAGGTGTTTGCAGTTATAAGTAAGACCAATATCAT ATCTGGAATATATATAATTACAAAAAAAATTAAATATTACAAGCGCGTATAAAGTAGTTTAGATTTTGAAACTGATTTTTTTTTTTCAAATATAAACAA ACCACATCTTTCTTTTGACTTCCTATTACCAATGTCCCAGGATAGAGATATATCGTCGTTGATAATAAATCAAACATTTAAGATCTATAAATCCAATTC ATCACTATCGTCATCTATAAAACTCACTAAAAAACAATTGGAGAAATTTCGAATAACCCTATGTAAATACTTGTACCAAAGATATCAACAAACTTTACA AATAGAAGATGATTTACTAGTTCAATATGAATTATATCGTGATATTGTACCTGAACTCACTATTTATAACAATTCAATTGAAGAGCATTCACAGTATAA AATCCAATATCTAATAATCAAATGTGCCAATAATCGATTGATATCTCGTCAAGTGTTCATATTTGTTAGTAATGATGATGATTTCACTTTGGTATTGAA TAGAATTACCAATCACCCTAGCATAAATGAATATATTTTGGAATTATTAGAAGATATTAATGATTATCCATTGATTATAAAACCACTTGATTTACCTGA TTATCTAATACCCAACTTGGTGGATCAATTGGGTAATAAGTTGGATTCTTTGGGTGATTTACAATTAGTATATTCATCTTCTACATCTAATAGGTTGAG AAATTTTATAATTGATATTCCGAAAAACGATTTACCAAAATTGTCTAGTGATGGGAAATTATATCAAGATATTGTTAAATTCATGTATAATAATACCAA AATAAAATTTGAAAAATTAAGAATAGAAAAATTTATTAATAATTTAATTAATATTGCTAGTGATGGGAAATTTAAATTAATAATTAATGATAATCAATT AATTTGGTTTTTGATTGAATCTATAAAATCATCTTTACAATAAGTAGTAAGTGTACGTGGTCAATAAAGTAATGTATATATGTGCGTGTACTGAGTGAT GATTTTCTATTTTATCTCGCGCGTTGTTATTTTTGTTGTTATTGTTGTTGTTGTCTGAGACTTAATTAATAACTTACGTGTTCACTTACATTTATTTGC TTATGAGCCTAGGCGTTATTTTTTTTTTCTCTCTCTCTCCCACTTTCTACTCTTTGAACAATGTGGTGAAAAAAAAAAAAAATAGAACCAGATCTTTTT TATTCCATATATTTCAAAACCGACTTGTTTCTTTTTTTCTTTTACAACCAATTAATACAAAAGAAAGACAACAATCATAATGTCTACTTCTGTTGAACC CAATGAAACAGAAGCTTTGTTGAGAAAGCAGAATGATCTTTCCACAACTGCCTCAATTGAAGAAAAATATCCTCACCAACAAGGAGAGGCTGCAGAAGA CGATGACGACACTCTTAAAAGAACCCAATATGATGAAGCTAAAGAAACCGCTGAATCTTTAAAACAAGTTGAATCGATATTAGCACCTATTGTTTTCAC TGCATTGTCATTTTTCGTGAGATTTTATCGTATTTCAGTGAATGACCATGTTGTTTGGGATGAAGCTCATTTTGGTAAATTTGGATCCTATTATTTACG ACACGAATTTTATCATGATGTTCATCCTCCATTGGGTAAAATGTTAGTTGGTTTATCTGGTTATTTGGCTGGATACAATGGATCTTGGGATTTCCCAAG TGGTGAAAAATACCCTGATTATATTGATTATACTAAAATGAGATTGTTTAATGCCACTTTCTCTGCCTTGTGTGTACCATTGGCTTATTTCACTGGGAA AGAAGTTGGATTTTCCATGTTTACTACTTGGTTATTTACTTTGATGGTGGCTCTTGAATCAAGTTATGTCACTTTAGGTAAATTCATTTTGTTGGATTC AATGTTGTTATTCTTCACCGTTGCTACTGTTTTCTGTTTTTCACGTTTCAACAATTTTAACAATAAATCACAAGAATTTTCTAGAAAATGGTGGAAATG GATTCTTTTAACTGGTGTTTCCATTGGTTGTACTTGTTCTGTAAAAATGGTTGGATTGTTTGTTACCACATTGGTCGGGATTTACACTGTTGTTGACCT TTGGAATAAATTGAGTGATAAATCTATTTCATGGACAAAATACATTCAACATTGGTTTGCTAGAATTGTTGCTTTGATTCTTGTCCCAATTTTCATTTT CATGCTTTCATTTAAAGTTCATTTTGATTTGTTGTATAAATCGGGTACTGGTGATGCCAATATGTCATCACTTTTCCAAGCTAATTTGGCTGGTTCCGA TGTTGGTGGTGGCCCACGTGAAGTATCCATGTTCCACTCGGTTATCACTTTAAAGAATCAAGGTTTAAGTGGTGGCCTTTTACACTCCCACGTTCAAAC ATTCCCAGAAGGTTCAAAACAACAACAAGTTACTACTTATGGTCACAAAGATTCAAACAACAATTGGATTTTCCAAAGAGCTAGAGGACAACCTTATTA TGATACTTCTGGTAACACCACTGACATTGAATATATTTTTGACGGTATGCATGTAAGATTGATGCATCCACAAACTGGTAGAAACTTACATACTCATGA TATTCCAGCTCCAGTGTCTAAATCTGAATATGAAGTTGCATGTTATGGTAATTTGACTATTGGTGATCCTAAAGATAATTGGACTGTTGAAATTATGGA ACAAGCAAGTGATGAAGATAAAATGAGATTACATCCTTTGACTTCGTCATTTAGATTGAAGAATGAAGTGATGAATTGTTATTTGGGGGTCACTGGTAC TACATTACCTCAATGGGGGTTCAGACAAGGTGAAGTTGTTTGTTACAAGAACCCATTTAAAAAAGACAAGAGAACTTG ÚKOL 3 Charakterizujte část genomu Candida albicans (kvasinka) rovněž pomocí heuristického modelu. PREDIKCE GENŮ U MNOHOBUNĚČNÝCH EUKARYOT Mnohobuněčná eukaryota se vyznačují komplexní organizací genomu, geny jsou separovány dlouhými intergenovými úseky, geny obsahují mnoho intronů, i velmi dlouhých. Exony/introny jsou identifikovány pomocí míst setřihu (GT na 5´konci intronu, AG na 3´konci). Vzniká velké množství chyb! Dlouhé introny jsou určeny jako intergenové úseky, krátké intergenové úseky jako introny, krátké exony nemusí být identifikovány. ÚKOL 4 Analyzujte část genomu mnohobuněčného eukaryotického organismu pomocí programu GeneMark (http://exon.gatech.edu/GeneMark), verze pro eukaryota. GATCGGGGTATCCCAGCTGCTAATTCCCCTCCTAACAGCCCTGCAGCGCCTGGATTGGAGGTGTGACAGGTGGCATTTTTGGCCCGAAGTCCCTAGGCA GGACGGGTGGCTGTAGCCTGCGCGTTTGTGTAAATTAACCTCCCTTTTTAGTTCTGTGAAGGGAGAACGTGATTAGTTTAAAATCACCTGCGTGAAAGA GGCTCTTGATTAGTTTAAAATCACCCACGTGAAAGGGGCTCTTGATTAGCTTAAAATCACCTGGGTGTTTCGTTTTATCTCAGTTGGCCTCTGAATGTT TATCCTACAGCTTCCTGCAAGCTCCAGTCTACCTTCTAAGGGAATAACGGGGCTCGTCTTTCTACGTTACTTTATGTGGGAGTAGTTGTAAAACAGAAT GAGGGAGACCGCGGGGATTGTGCGGAAATGTAGCCACCGTTTACATGTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTTGACACCAGCC TGGGCAACATGGTAAGACCCCTTCTCTACCCTCCCCCCCAAAAAAAAAATTTTAGTGTGGCATGGTGGTGGGTGCCTGTGGTCCCAGCTACTCGGGACG CTGAGGTGGGAGGATTGCTTGAGCCCAGGAGTTTGACACCAGCATGGGCAATATGGTAAGACCCCATTATCTACAAAAAAAAAAAAAAAAAAAGAAAAG AAAAATTAGCCGGGCGTGGTGGTGGGTGCCTGTGGTCCCAGCTGCTCGGGACGCTGAGGTGGGAGGATTGCTTGAGCCCAGGAGTTTGACACCAGCATG GGCAATATGGTAAGACCCCATTATCTACAAAAAAAAAAAAAAAAAAGAAAAGCCGGGCGTGGTGGTGGGTGCCTGTGGTCCCAGCTGCTCGGGACGCTG AGGTGGGAGGATTGCTTGAGCCCAGGAGTTTGACACCAGCATGGGCAATATGGTAAGACCCCATTATCTACAAAAAAAAAAAAAAAAGAAAAGCCGGGC GTGGTGGTGGGTGCCTGTGGTCCCAGCTGCTCGGGACGCTGAGGTGGGAGGATTGCTTGAGCCCAGGAGTTTGACACCAGCATGGGCAATATGGTAAGA CCCCATTATCTACAAAAAAAAAAAAAAAAGAAAAGCCGGGCGTGGTGGTGGGTGCCTGTGGTCCCAGCTGCTCGGGACGCTGAGGTGGGAGGATTGCTT GAGCCCAGGAGTTTGACACCAGCATGGGCAATATGGTAAGACCCTATTATCTACAAAAAAAAAAAAAAAAAAAAAGAAAGAAAAATTAGCCTGGTGTGG TGGTGGGTGCCTGTGGTCCCAGCTGCTCGGGACGCTGAGGTGGGAGGATTGCTTGAGCCCAGGAGTTTGACGCCAGCATGGGCAATATGGTAAGACCCT ATTATCTACAAAAAAAAAAAAAAAAAAAAAAGAAAGAAAAATTAGCCTGGTGTGGTGGTGGGTGCCTGTGGTCCCAGCTGCTCGGGACGCTGAGGTGGG AGGATTGCTTGAGCCCAGGAGTTTGACACCAGCATGGGCAATATGGTAAGACCCCATTATCTACAAAAAAAAAAAAAAAAAAGAAAAGCCGGGCGTGGT GGTGGGTGCCTGTGGTCCCAGCTGCTCGGGACGCTGAGGTGGGAGGATTGCTTGAGCCCAGGAGTTTGACGCCAGCATGGGCAATATGGTAAGACCCTA TTATCTACAAAAAAAAAAAAAAAAAAAAAAAAAGAAAGAAAAATTAGCCTGGTGTGGTGGTGGGTGCCTGTGGTCCCAGCTGCTCGGGACGCTGAGGTG GGAGGATTGCTTGAGCCCAGGAGTTTGACGCCAGCATGGGCAATATGGTAAGACCCTATTATCTACAAAAAAAAAAAAAAAGAAAGAAAGAAAGAAAAA TTAGCCTGGTGTGGTGGTGGGTGCCTGTGGTCCCAGCTGCTCGGGACGCTGAGGTGGGAGGATCACCTGAGCCCAGGAGGTGGAGGCTGCAGTGAGCTT AGATTGCAGCACTGTGCTCCAGCCTGGGTGACAGAGTGAGACCCCATCTCAAACAAGAAGCACCAAAAGGTGTCTGAGATCAGGCACGGTGGCTCACGC CTGTCATTCCAGCACTTTGGGAGGCCGAGGTGGGTGGATCACCTGAGGTCAGGCATTTGAGACCAGCCTGGCCAACATGGAGAAACACTGACTCTACTA AATGTGCAAAAATTAGCCGGGCGTGGTGGCACACACCTGTAGTCCCAGCTACTCCAGAGGCTGAGGTAGGAGAATTGCTTGAACCTGGGAGGTGGAGGT TGCAGTGAGCTGAGATTGTACCACTGCACTCCAGCCTGGGAGCTGAGATTGTACCACTGCACTCCAGCCTGGGCCCTAAAGCGAGAGCTTGACGGTGGG GTGGGGGTGTGGGATGGACCTTCTGTGGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTTGACACCAGCATGGGCAACA TGGTAAGAACCCATATCTACAAAAAAAAAATTTAAAAATTAGCCGGGTGTGGTGTGTGGGTGCCTGTGGTCCCAGCTACACCACGAGTGGAATTGTGAC GTGCACAGCCTGGGTAAATGGGGTTAGCTATATTCACCCTGAAGGGGGCACATACCCTATTATTTCTAACACCACTGAACTCAGAATCGGGACCCAGAT TCGCCGGGGCAGCGCGGGACGAGGCCTCTCCCGGGCTCCGTCTTCCCTGTTAATAGACTGAGAGACGGAATGAGAGCTTCGAAGGTGCTGGGGGAGGAG CATTGGGTGTCCCCAGGTGGTGAGCTTGGGTGTGTGTGTGTAGCCGACAGTCTCCCAGGCTCAGTATTCCCTGTTAATAGACTGAGAGACGGAATGAGG GCGTCCCCAGGTGGTGAGCTCAGGGACGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAGCCGTCAATCTCCTGGGCTCCTGGGTT CCGTCTGCCCTGTTCATAGAGACAGAATGAGGGCGTCCCCAGGTGGTGAGCTCAGGGACGTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG TGTGTAGCCGTCAATCTCCTGGGCTCCTGGGTTCCGTCTGCCCTGTTCATAGAGACAGAATGAGGGCGTCCCCAGGTGGTGAGCTCAGGGACGTGTGTG TGTGTGTGTGTGTGTGTAACCATCAATCTCCTGGGCTCCTGGGTTCCGTCTGCCCTGTTCATAGGGATGGAATGAGGGCGTCCCCAGGTGGTGAGCTCA GGGACGTTGTGTGTGTGTGTGTGTGTGTGTGTAACCATCAATCTCCTGGGCTCCTGGGTTCCGTCTGCCCTGTTCATAGAGACGGAATGAGGGCGTCCT CAGGTGGTGAGCTCAGGGACGTGTGTGTAGCCATGATTATCCTTTAGTACCTGGTCCCCGTGCCCTCCAAGCCATCCAAGGGCTTCAGGAGGCCGCGCA GAAGGAGGGTCGGTAAGCGAGTCCACGGACCACCACGCAGGCCCTGACCCCGCCAGGGGCCCTTTGGAGGGTGAGCCCTGCGTGTTGGGGGGATGATGG CTCCGGAGGGTGAGCTCTGGGATGAGAGGGGACGTGCAGGTGGCCGCATTGCAGACACAGCTGGCTCGGGACCTGGGCGCCATCTGCCGTCGACTCAGC TCGCTCCTGAGCTGTGCCTCTTCCTTGCCTCACGTTGGAGATAAAAAGCCATAGATGGCCGGGGGCGGTGGCTCAGGCCTGTCATCCCAGCACTGTGGG ATACCAGCGCAGGAGGGTTGCCTCAGCTCAGGGGTTCGAGACCAGCCTGGCCAGCATAGCGAGACCCTCTTCTCTGTAACAATTTTTTTTTCTTTTCTT TTTTTTTTTTTTGAAACAGAGTCTCGCTCTGTCACCCAGGCTGGACTGTAGTGGTATGATCTCAGCTCACGGAAACCTCTGCCTCCCGGGTTCCCGGGT TCAAGTGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCAGCTGCCACCATGCCTGGCTAAATTCTGTATTTTTAGTAGAGACGGGGTTT CTCCATGTTGGTCAGGCTGGTCTTGAACTCCCGACCTCAGGTGATCCGCCCACCTCGGCCTCCCAAAGTGCTGGGATGACAGGCGTGAGCCACCGCGCC CGGCCTCTTTCAGGTTTTTATATGTAACTTTGCCACTCAGTCGGTACTGAAGCCGTTATGGAGGCCTGCCTGGTGAGATGCGGCTGCCACAGTGTGGCT GGTATTCGAAACACACAGGAGGCCGCAGCCAGCTTAGCAGAAGCACCAGGGCTTTCGGAACTTGGTTTTGAGAGTTTCCTGTTTTCTTGACTTTCATAT ACCATGGATGGTAATAACTGTTGGGGGGAAAAAGCTATTAAAGATTCCTGTGGATGGTGAGATGACCTTGGTTTCAGAAACACCCAGACACAGAGAATT GTGCATTTTAGAATCCAATAAATCTACGCTGGGCACGGTGGCTCATGCCTGTCATCCCAGCACTTTGGGAGGCCGAGGTAGGTGGATCACCTGAGGTCA GGAGTTCGAGACCAACCTGGCCAACATAGTGAGAACCTGTCTCTACTAAAAATACAAAAAATTAGCGGGGCGTTGTGGCAGGTGCCTGTAATCCCAGCT GCTCTGGAGGCTGAGGTGGGAGAATTGCTTGAACCCGGGAGGCAGAGGTTGCAGTGAGCTGAGACCATGCTGCTGTACTCCAGCCTGGGTGACAGGGCG AGGCTCCATCTTAAAAAAAAAAAAATCTGATCATTTACCTGTGCACCTTCACAAGTGTTTAAAAAAAAAAAAAAACCGGGCACGGTGGCTCACGCCTGT CATCCCAGCACTTTGGGAGGCTGAGGTGGGTGGATCACGAGGTCAGGAGATCGAGACCATCCTGGCTAATTCGGTGAAACCCCGTCTCTACTAAAAATA CAAAAAATTAGCTGGGTGTGGTGGAAGGTGCCTGTAGTCCCAGCTGCTCAGGAGGCTGAGGCGGGAGAATGGCGTGAACCCAGGAGGCGGAGCTTGCAG TGAGCTGAGATTGCAGCACTGCACTCCAGCCTGGGTGACAGAGCCAGACTCTGTCTCAAAAAAAAAAAAAAAAGATTGGGTTGATATTTTACCTACTAT AATCTTATATGTTTGCAATTTCCTGTCATTGAGTTTTATAACTTTCTGCCCTTTGCTGACAAAAACATTTAAAATGTCTCATGGCAGCTTGTGTTCTTA AAATTCAAACTGTTTATCCATAGCCCTTGAGAGAAGGGTTTAATGTGAGTTGGTTGGGAGTGACCGTTGTCTGTTTTTTTGAGACAGAGTGTCGCTCGC TCGCCCAGGCTGGAGTGTAGTGGCGCGATCTCGGCTCACTGCAAACTCTGCCTTCCAGGTTCAAGTGATCCTTCTGCCCCAGCCCTCCAGCTAGCTTGG ATTACAAACATGCACCACCACACCTGGCTAACTTTTGTATTTTTGGTAGAGACAGCGTTTTACCACGTTGGCCAGGCTGGTCTCAAAATCCTGAGCTCA AGGGATCCGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGGCACGACGTCAGGCCAAGGGGGTGGCTGTTTTATAACAGGTTTGCAAGT TTGTTTTTGTTTTTGTTTTTCTTTTTTTTTGAGGTGGAGTTTTGCTCTTGTTCCCCAGGCTGGAGTGCAATGGCAAGATCTCGGCTCACTGCAGCCTCT GCCTCCGGGGTTCAAGGGATTCTCCTGCCTCAGCCTCTCGAGTAGCTGGGATTATAGGCACCTGCCACCATGCCTGGTTAATTTTGTATTTTTAGTAGA GATAGGGGTTTTTCCGTGCTGGCCAGGCTGGTCTCAAACTCCCGAACTCAGGTGATCCACCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGA GCCCCCGTGCCCAGCCAGGGGGTTGCTGTTTTATAATAGGTTTGCAAGTTTGTTTTTTGTTTTGTTTTGTTTTGTTTTTTTGAGACGGAGTTTTGCTCT TGTTGCCCAGGCCGGAGTGCAATGGCACAACCTTGGCTCACTGCAACCTCCGCCTCCCGGGTTCAAGCTATTCTCCTGCCTCAGTCTCCTGAGTAGCTG GGATTACAGGCGTCCGCCACCACACCCGGCCAAGGGGGTGGCTGTTTTATAACCAGTTTGCAAGTTTGTTTTTCTCGTGTGTTAGAGGAAAATCTCTTC ATGATATGGTGAAGCCGTCGTCTGTTTTGGAAGACGGTGGCCAGGAAAATGGTGGTAAGTTCTCCTGAAAGCAAACGCAGAAGGAAATAAAAATGACTG ACAATTAGATCGTACAAAGATGAGCATTGTTTTTATTTATTTTTTTTGAGACGAAGTCTTGTTCTGTCACCCAGGCTGGAGTGCAATGGCACGATCTTG GCTCACTGCAACCTCCGCCTCCCAGGTTCAAGTGATTCTACTGCCTCAGCCTCCTGAGAAGCTAGAATTACAGGCATGCTCCACCACTACCCAGCTAGT ATTTGTAGTTTTAGTAGAGACGGGATTTCACCATGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCACCCACCTCGGCCTCCCAAAGTGC TGGGATGACAGGCGTGAGCCACCGTGCCCCGGCCCCGGATTCTATTTCTTAAACTGCAGGAAATACTAAATACTTTACATGCGTATGTTTCTCATTTCA TTTTCCTACAGGAATTGGAGAGTGAGTTGCTGAGATTTCATTTTTATTCTCTTGGTCCTCATCATGCAAATGGCTAAATGGTTTTTTTTTGAAACAGGG TCTTGCTCTGTCCCCCAGGCTGGAGTGCAATGGGGCGATCTCGGCTCACTGCAACCTCTGCCTCCCGGGTTCAAGCCATTCTCCTGCCTCAGCCTCCCG AGTAGCTGGGATCACAGGTGCGCACCACCGTGTCCGTCTGATTGTTAAATTTTTCCTAGAGACGAGGTTTTGCCACGTTGCCCAGGCTGGTCTCAAACT CCTGAGCTCAGGTGATCCCCCCACCTCGGCCTCCGAAGGCGCTGGGCTTCCAGGCGTGCAGAGGGCTCTTTGTCTACATCCGTCACTGGATTTTCTACC CACGGGGACATGTGACCCCAGCGAGGTGGTCGGGGAGTCGTCGCCGGCCAGCGCTGCAGCCCAGGGATGATGACGGCTCTAGGAGTTTGGTTGTGGAGC ACAGATGCCGGCGGTGTGGGCACGAGGCCCTGCACCTGCGTTTCTCCTGCACGGGCCCCGGAACCCCGGGATGGGATCTCTGTGGAGTGATCACGTCAG TGGGCTCTCGGCGAGGCTGGCGGGTGGGAGTCGGCGGCATCCGGGCTGGGCCCCCGAGGCTTGGCTCCCTCTCTGCACCCCAGGGGTCCCACAGGCTGG TGACAGCGCGACTGAAGGCCACGCTGCAGTCAGGAGGCCGCCCCACGTGTGACCCCGGGTCTTCCCACCCCGCCCTGACCCAGGGTCCTGGCCTTCCAC GTGTGACCCTGGTTCCGGCACCTGTCCCCGCCCCCCCATCCTGTTCCCCTCCTCTCTCCCTGCCCCCCCTCCCCTCTCCCCGCCCCCCCTCCCCTCTCC CCGCCCCCCTCCCCTCTCCCCGTCCCCCTCCCCTCTCCCCGTCCCCCCTCCCTCTCTCCCCGTCCCCCCTCCCTCTCTCCCCGTCCCCCCTCCCCTCTC CCCGCCCCCCCGTCCCCCCTCCCCTCTCCCCGTCCCCCCTCCCCTCTCCCCGTCCCCCCTCCCCTCTCCCCGTCCCCCCTCCCTCTCTCCCCGTCCCCC CTCCCTCTCTCCCCGTCCCCCCTCCCTCTCTCCCCGTCCCCCCTCCCTCTCTCCCCGTCCCCCCTCCCCTCTCCCCGCCCCCCCGTCCCCCCTCCCCTC TCCCCGTCCCCCCTCCCCTCTCCCCGTCCCCCCTCCCCTCTCCCCGTCCCCCCTCCCTCTCTCCCCGTCCCCCCTCCCTCTCTCCCCGTCCCCCCTCCC CTCTCCCCGCCCCCCCATCCCCCCTCCCCTCTCCCCGCCCCCCCTCCCCGTCCCCCTCCCCTCTCCCCCTCCCCCCTCCCCGCCCCCCTCCCCTCTCCC CCTCCCCCCTCCCCGCCCCCCTCCCCTCTCCCTGCCCTCCCCTCTCCCTGCCCCCCCTCCCTGCCCCCCTCCCCTCTCCGCCCCACCTCTCCCCTCCCC CCTCCCCCTCCTCTCTCCCCCCTCCCCCTCCTCTCTCCCCCTCCCCCCCTCTCTCCCCCTCCCCCTCCTCCTCCTCTCTCCCCCTCCCCCCTCCCCCTC CCCTCTCCTCTCTCCCCGCCCCCCTCCCTCTCTCCCCGCCCCCCTCCCTCTCTCCCCGTCCCCCTCTCCTCTCTCCCCGTCCCCCTCTCCTCTCTCCCC GTCCCCCCTCCTCTCTCCCCGTCCCCCCTCCTCTCTCCCCGTCCCCCCTCCTCTCCCCGTCCCCCCTACTCTCCCCGTCCCCCCTCCCTCTCTCCCCGT CCCCCCTCTCCCCGTCCCCCCTCCCCCTCTCCCCGTCCCCCCCTCCCCCTCTCCCCGTCCCCCCCTCCCCCTCTCCCCGTCCCCCCCTCCCCCTCTCCC CGTCCCCCCCTCCCCCTCTCCCCGTCCCCCCCTCCCCCTCTCCCCGTCCCCCCCTCCCCCTCTCCCCGTCCCCCCCTCCCCCTCTCCCCGTCCCCCCCT CCCCCTCTCCCCGTCCCCCCCTCCCCCTCTCCCCGTCCCATCTCTCCCTTGAGTCCTTGGGTGGTCCTGGCCGCACGCCTACCTGGCGGCCCCATCCGT CCCTTCAGGGCAGGGACAGCCGATGCCCCGCACCTCCCCCATCGCTTTTCTGCCTCTGCCCCCTCCCCACATAGGCCTGGCGTCCCCTGGACTCCTGTA GGCCGAGCTGTAGTCTTAAGAAGTGTGTCCCGCAGCCCTAAAAGGTTGGGGTACCCTCCGGCCGTCATGTTCTGGGCACCCTATAGTCCTAAAGGGTCA GGGCACCCCATGGCTGTCATGTTTAGAGTACCCCGTAGTCCTAAAGGATTGGGGCACCCCACATCCCCGGTGTTCAGGGCACGTCATAGTCCTAAAAGG TCGGGGTACCCCTGGATGAGGGGCGGTCAGGAGGGTCAGGGCCTGGGTGGACATCCTGTCATCCTACCCTGTGGGCTATGCCCTGTGTCCCTGACTCTG ACATTGGGTGAGGTGAGGACGGGGCAGGTTTCCATGGTACATTTATTTATTTTTTGAGACGGAGTCTCGCTCTGTCGCCCAGCCTGGAGTGCAGTGGTG TGATCTCGGCTCCCTGCAAGCTCCGCCTCCCGGGTTCATGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATCACAGGCGCCCGCCACCACGCCC GGCTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGTCTCTAACTCCTGACCTCGTGATCCGCCTGCCTCGGCCTCCCA AAGTGCTGGGATGACAGGCGTGAGCCCCTGTGCCTGGTGGGACCCCAGTTTTCTATTAAAAAAAGGCCGGGCGCGGTGGCTCACACCTGTCATCCCAGC ATTTTGGGAGGCCGAGGCGGGTGGATCACTTGAGGTCAGTTGTTTGAGACCATCCTGGCCAACATGGAGAAACCCCGTCTCAACTGAAAATACAAAAAT TAGCCGGGCGTGGTGGTGGCGGGCGCCTGTGATCCCAGCTACGCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCAGAAGGCAGAGGCTGCAGTGAGC CGAGATCGCACCATGGCAGTCCAGCCTGGGCGACAAACAGAAAACAGGCACAGCATAGAGTCTGCCTTTCTGGAATCGCTATAGCTAGGGGGCTGCCTC CCTAAGCCCCACTTCTCTGAGTCTCCTGCAAGGTTTGTGTGAGTGCCTGGGGGCCGAGGCCCTGGCGAGGAGGATGGGGGCTCGGGGGCCTCTGTGACC TGGGGGAGGGGCTGTCGCGTCCCCCTGTGGTCGCGTCCCCCTGTGGTCGCGTCCCCCTGTGGTCGCACCTGTGGGCTGTCGGCCGTAGGTACCGCTCAG GCCCGTCCTGGTTGCGGGGCAAGGGCCTGTGTGTTGGGGGCTGAGAGACGGGGCCTAGAAGCCCTGTTGTGGGGAGGCCTGTGGGGGGCTTGTCCAAGC GGGACCTCCTATCCCTGCACCACTGGGGTCGTCCCCCCCGACGGGGCCTCGTCCCCCCCATGGCTGCTCCGTGAGGTCAGGCAGCGCCGCGCTAGGGAG ATGGGGTGGCCCTGTCCCCGCAGAGGGGCTGTTCTCTGGGGCAGAGGCGGGCAGGACCCCCCGCCTTGTGGTCAGGACCCCCCTCCTTGTGGTCAGGAC CCACTCGTGGCGGCCGGGGCTGCTGCGGGAGCTGATACCGGGGTGGAGACAGCTACGCCATGGTGAGGACACGGGGCCGGGGGAGGCTGCAGCCGACGG CCAGGCCCCCGTGCGGAAGACCCGGGGCAGCCCCCGCCTAGGAGCTGCGCAGACACGTCCTGGGGCCGCTGTTTCACCGAGACCCGAACGGCTGCTCCC CAGGCTCTGAGTGGCGTGTGCGGCAGCCAAGGCTGGGGGAGCTGTGACTTCTACACGGCGCCAGGGCGCAGGGATCCCTGCTGTGCCAGGGTCTGCCCG TGGGTCATGGGGAACGTGGTTCGAGGCCCCTCTGCTGTAGCCACGCAGCAGCGTCTTGCCGGGTGCGTGGCCTTGTCTGCAGGCTTGAGACCCAGCGGG GCCGTCTGTGCCACGTGGGGTCTGCGCCGGCCCAGGGGCCGCACCGCTGTCAGCCACAGGATGTGTGTCTCGGGGCTGCGGCAGACGGGGCTTCTGTGC CGTGTGGGGTCCGACCCCTCGGCCTGGGCAGGGCGGCCCGTGGGCACCGTGCTGGGGACGACCTCCGGCCTCTGCTCCTTCCCCGGCTCTGAGTCATAA AGGCCTCCCGCTCCCCCGGGAGCCGTGTCTTCTCTAGGAACTGCTGAGCGCGGCTGGGTCTGGCTCTGCCTCATTCCTGCATCTGATGCCCGCCCGTTC TCCCCCAACTCCCCTTTCTCCATCTCCTCCCTCCCCTCCGTCTTGCCCGTCTCCATTTCCTCCATCTCTCCCTTCTCCGTCTCCTCCGTCTCGTGGCTG TCCAGACCCCGTCCCTCCGCAGGCTGGGTCCCCCCTTGTTCTGGGCGGGGCTCTCGGTGCCGGTCTGGTGCCAGTGCTGGGAACCTGTGGTGCCCGTCA CCTCTGCTCCCCGTCACCTCGGCTCCACCTCGAGGAGCTGTGTCCTGTCCCACGAGGAGGCGCCCCGGGACCTCAGGGCTGCAGGCCATGGCTGGGTCC GGCTTTGCCTCATCCCCTGTATCTGAGGCCCCCCAGTTCTCCCCCATCTCCCCCTCTCCATCTCCTCTGTCTCCCCCGTCTCATGGCTCTCCGGGCCCC GTCCCCCCCACCAGGCTGGGTCCCCTTATTCTGGGTAGCCCGTCTTGCCATCACTGTCCCTGCCGCCCCTCAGGACAGCGCTGTGGATGCCGGTCTGGT GCCGGCGGTCACCCCTGCTGCGCCTTGAGCAGCTGTGTCTTGTCCCGTGAGGAGGTGCCCCGGGACCTCAGGGCTGTGGCTGGCACCTTTCATGGTCCT CCCGGAACAGAGCTCATGCTGCTGCTCCTGGGTGGACCCGGGGCTGCCCCTGTGAACACTCTTGGTTCTGACACGAAGGCACCCCGTGAGGGAGGGGAG GGGTGGGCTGGGTCGGGTCCCTGCTCGGGGATCTCCAAGGGGTGACGGCCACGAAGTGCGGGGGACCCACGATTGTCATTCAGAGGGTGAGGCGCTGAG CCCTGGAACATCCTGGCTTCTGGCGGGACGCTGGCCACGGTCAGGGTGCCCCGAGTGCTTGTTTTGTGGGGTCCCTGGCCCCCTGCACACGTGAGCCCT CGGGGGAATGGACCCCCAGAACACGTGGAGGGCATGACTGCTGGGCTCTGGGTCTCCCCGCAGCGCCGCCGTCCACGAGGACCCCCGACAGGACCCCCC CACTTCTCCGGCAAAGGTGTCGGTGTGCACGCCCGGCCCCGGCACTGCCCGGGGCATCTGGGGTTGTGGCTGGGGAAGGACGGGGCACTTGGGGGAATG CATTGGGTACAGGGATGTCGGAGGATGCTCAGTGGCTGTGCAGGGGCCTCACCAGCGTCGTGGGTGGGGGACGCTGTCTTTCACGGCCCCAGGGAGGCC TCTCATGTCTCAGCCTTGAACCCTAATTGGGGGGGCTCCCAGTGTCAGTGTGGGGAGGCTGAGGGCGCTGGTCAGAGCCCTCCCATGACCATGGGCTCC GGCTCATAGGTGTGTGTGTGTTGGGGGGTGGTGTCCGGAGGTGGTGTGGGGCGGACATGGTCTCCCAGACGGGCTCCCCGGAGCTGAGGGCAGGGTGGG CCCTGCGGGCCGTGGCTCCCAGTGTCATTTCCCTGCGGCAGAACCTCCTGCCCTGCACCTGTCCTGTTGTTGGGGGTCCTGACCCTTCGCAGACCCAGG TGGGGGCATCCGGGAATTCAGGGGAGCACCTGGTGACACTGAGGACACCTTGGGGACAGCAGCATTCCCCCGGGAGGTGCTGCCTCGTCTACCCACCAC CTGGAGCCCCCGCAGTCTCTCCTCAGGGGTCACGGGACCACCCTGTGCGTCCGCCTCCTTCACAGCGACGGCTGCCGGTGGATGAACGGGGTGCGTATG CTGCTCTCGGGGTGGTCCCGGCTTGGCCCTGCCCTGCCGCGTGGACGCTGCCACCTCCGTTTCCTGGGGGAGAGTTTTGTCGTGTGTGAGGACGGGGTT GTTCCCATGGTCACCACCTGTGACCCTCACCTGCGTGCAGAGGGAGCACAGCTGGGCCTGGGCTCATCCACGTCGGGTGCCCCTGGGGTGCTTGTGGCC TCCCCTGCACAGGGAGCTCCTCCTGGTGGGGCCTCCGACCCCCCTCATTTAGGTGTCCCCCAGCTGTGGACGTCCCCACTCTGGCGCGGGCTCCTTCCT TCTGGGGTCGTTTGTGGAACGGGGCCTGGGTCCGTGGCTCCGGGAGGGAACGGCCCCGAGGGTGGGGTCCCCCTGGCTGGGGAGGGGCCCCGCAGAGAG GCCCAGGCTGCACCCCGAGGCGTCTGGCGTGGCTGCGGTCCCCCTCCAGCACTGACGCTTGTAAATCCTGGACGGAGCCACGCTGTGGCTGAGGGAGCC GAGCCCGTGACTGAAAATCCTCCAAACGTACAAGCGTGATCGCCAGAAACGGTTTTGTACGTTTACACAAAACATTCACACAGCCTGTGGTGGAGGCTC CTGTCCAGGACTGAGGCGCCCGGGAGCCGCCGGTCACCGTTGTGCGCACACGGACCCTTTCCACAGACGCAGGCCCCGGAACCCAGGCTGGGTCGGGAA CGGCAAGCGCCAGAGGGTGTCCGTGTGGGAACCCGTCCCATTCACGCGCGGCCCTACGTGTCCCCCTGGCACAGAGCTCTGGGCAGGTCCAGCCACGAA CCCACAGCGGCAATCAACACGCTTCTGTGAATAAATAAAAGTTTATCATTCCGTACAAACGCACTCATTTTCCACAACAGTTTTTACACGAGCCGCGGT GGCCCGGTGGTGGCACGTGGGGAGCGGCCCCGCGGCGGCGTTCTCGCGGGCGGCGTCACAGCGGCTCCAGGTCCTCGTCCCCGCATGCGTACTCGTACA GGTCCACGGCGCCCAGCGGTGAGGGCGCCTCGAAGAAGGGCCTCTGGGCCAGCGGGGAGCGCAGCGCACTCAGCTTCTGCTCCACAGGGCTGAGCTCGG CCTCGAACCTGCAACGAGGGGATGGCGAAGACGTGGCCAGCAGCGCGGAGCAGGCCCTGGGCCGTTTTCCGGGAAACACAGGCTGCTCGGCCGCCAACC TCCAGCACGAGACAGTCCCCTGAGCCGACCTTGAGCTCCAGCCGCTGAGCCAGCAACGCGCTCCTGGCTGAGGACGCCGGCTCCACGAATGCTCCCGGG GGAGGGGCCTGCGGTGCAGGTGAAAACCCACCCAGGACGGGACGGAGCCCCGGGCGTGAGCTACACACAGAGGACCTGGGCGGGCGCGCCTCTTCCCCA AAGCGAGCTGCACGGGACCTGTGTTTACAACTGGGTCGTGGTGTCCACACAGGACTGGAGAACAAACAGGACCACAGTTTAGACACATTCTCCCAAAGC ACAGAAGCTCCCAGTGGAGGCTACCACTTCCGTTCTGACCAGAACACGCAGCAGTGCGAGGACTGGACGCGCGCCTTCCCTTAAGGTCTCGGCAGTGAC GTCGGGCGCTGCTCCGGGGAACCCAGCGCGGCCACGGACCACACAGACTCTCAGAGATCCTGTGGGGACACCTCTGCTTTCCAAGACGCCCCCGACAGC CTGGCACTGAGCTCCCCGCGGTCACCCCCACGGACCTGAGCTCCCACTGCCGGAGCAGGCGTGGACACGGTGAAGGCAGTCGACCCCAGCGTGCTGCTG GCTGTGCCGCAGACACAAAGCCGGGCAGCCGTGGGGGTGGACACTGGTCAGGTGGGGAGCAGAGGTGACAGACGGTGGTGGGGACAGGCGCACACCCAT CCAGGCTAGGTCTGGGGGCCACAGCTGCCTTAGGGTGTCCGACAGGAGAAGGCAGAGACTGGGAAGTTGTGGGGACCCAGAGGTTGGCTGCCAGGTCAG AGCCACCGTGCAAACAATGACCACGAGGAGGACGGACACACAGGACAGACGGCCCAGGGTGGGCCGCAGGGGCCCTGTGCTGCTTGGCAGGAATCGGAC GGGGCGGCTGATGGGGGAGCAGGGGTTAAAATGCACGTCCATGGAACCCATGGGTCGGGAACCCAACGGTAACCAGAAAGTATTTTGAGCTGCACCAAA ATCAGAAGGCCGCGGGCTGCGGTGTCCATGGCCCACGTCGCTGCCTGAAGATACTCAAGGAAAACAGCAGAACAAAGGGGAGAAACACCCGTGGTTCCC TGGGAAGAGGAGTGAACCTCGTCGGCCCCTCGCCTCACTGATCACACACAAAGTCAGTATCAGGAGCGTGAGAGACGCTATCACTGGATTCTGCAGACA TCAGAGTCACGAGCAGGAAATACCAACAATAACCCACTGATGGGAACGGGCAAGTTTCCGAAACACACAAACCAGCAAAGCTCACTCAAGGCTGGGCAG GGGCTCACACCTGTAATTCCGGCACGTTGGGGGACCGAGGCGGGGAGAACCCTCGAGCTCAGGCATCCAAGACCAACCTGGGCAACACGGTGAAACCCC ATCTCTACAAAAAAATACAAAAAAGTAGCCGGGTGTGGTGGTGCGTGCCTGGAAGCCCAGCTACTCGGGGGGCTCAGGTGCGAGGATTCCTTAAGCCCA GCAGGTGGAGGGTGCAGTGAGCTGAGATTGCGCCAATGCCCTCCAGCCTGCGTGACACAGCCAGACCCTGTCTCCAAAAAAATAATAAAATACTACAGA TAATCTCTGCAAAGAGAATCCAGTAATATGTGAGGATAACACATGACTCGGCAAGATCTATCCTGGGAACACGAGGCTGGCTGAAAATGACTCGGTGTA ACTCGCTGTATGGACACACAGAGGAACCACGGAGCGAAAGCATCTGAGAAATTCGGCCCCCACTGCGAGAAAGCCCCCGCAGACCAGGAAGAGGGAGAA CATCCTCGCCAATGAGCGTGCTTACCGCTGCGTGAGGGGAGACGTCCCCCAGGACTGGGAACGAGGACGGCACACAAACCAGCAGGGCTCACTCAAGGC TGGCCGTGGTCTGCCCTGACATCCACCACATCTCCCTGCGGGTTCCAGGCCATGCAGCAGGCAAGAGAAAGGAAGTAACGTACACAGAGAGGTGAAAAG GAAGACGAGAGACGGACCCACTGACAGACATGGGCTGTCCACGCAGAAAACCCCAAAGAACCGACAGAGCTTTGAGATCCAGTAAGTGAGTTTCATGAA GGTGCAAAACACAAGGTCAATAAATTCTATATGCTACCAACGAACAACTGGAAACGGGAACCTTAAAAGCACCGCTGACAACAGGAAAACATCAGGAAA CACTCAGAAGTCTCACAACATTCGCATCAAGCGTACACGGAAAGCTACAAAAATCCTGCCGAGAGACACGGACGGCGCCCATGGACTGGGCCTCCTGCG GTTATGAAGGCGGCAGCTGGGCCCACAGGGATCCATTCACTCAGCGAGATCTCACTCAAAAGTTCAGATTGTTTGTTTTTGTTTTGGAAATGGAGTTTT GCTCTCGTTGCCCGGGCTGGAGTGCAGTGGCGCGATCTTGGCTCACTGCAACCTGCACCTCCCGAGTAGCTGGGATTACAGACGTGTGCCACCATCCCT GGCTTTTTTTTTTTGGAGACGGAGTCTCGCTCTGTCACCCAGGCTGGAGTGTGGTGGCGCGATCTCGGCTCACTGCAAGCTCCGCCTCCCAGATTCACG CCATTCTCTTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGCCCGCCACCACGCCTGGCTAATTTTTTTTTTGTTTAGTAGAGACGGGGTTTCACC ATGTTGGCCAGGATGGTCTCGATCTCCTGACCTCGTGATCCGCCCTCCTCAGCCTCCCAAAGTGCTGGGATGACAGGCGTGAGCCACCGCGCCCGGCCA GAAGGAGCCTATTCTTATGTGTTTTCATACAAAGGCAAAGAAACCAGAATAGCCAACGTAGCGCGGGACAGAAGCAGAACTGAGAAGCTCAGCACGGGA TGGCAAAAGTGACCACGTCGCTCCAGCGTGCACCACGGGAGGCCGGTGGGGGCCACAGTCCACGTGGTCCCGGGACCAGCACTCACCACCACGTCAGAG TCCAACACACGTTCCCACAGGACGGCAGAGCAAGGACGGGCTGAGTCTAAGATTCACACACAGGCAGCCCAACACCAGAATGGCCCCGACAGTTCCACA CGGCGCCCAAGTCGGAGGCTTCTCACCACAGCCAGCTCTGCCGCGGGAACGGACAGACGCGTGCAGCAGTGGGACGGAGGAAGGTTCCGGAACGGAGCC CGCACACGGCACAGTCACGACGCCGAGGCAACGCTGCGGCCTGCAAAGCTGATGCTGGGACAGTCGGACACCCGGACACCCACGTACCACAAGGGGAAC CTCAATCCGAGCTTCACACCCCACAGGGAAGGTTACTCAAGAGGACCCCAGACCAAAACACAAAACATCGCAGAGGAAAATCTTCGTGGCTTTGGATTC AGAAATGAATTCTTAGATATGACACCAGAAACACCGCTGTAAAAATAACCAGTCAGTGTCTCCTCCTCCAAATTCAGAACATCTCTGAGACACCACGTG AAGAGGGGGTGAAAACATACGTCACAAACTGGGAAAAAGGCGAACACGTCGCAAATAAAGGGTTTGTATGCTGAAGACACGAAGAACCCTCACGCTCCG TGAGAAAGCAACCAGCCCAGGGCCTCTCAGGGCCTCCAAGGCTGGAAGGCCGAAGCGGAGGCTGGAATGGCCACGGGCCCTGCAGCCTGCCCAGCAGGT GCCAGCCGTGGGATAAGGACAGACGTCGTTGGCGAAACACGACTCGGCCTCCCCGTGCTGGGCCTGATGTGCGAAGCCTCGCGCCCAGGATCACAGAAC CCCACGCGGGGAATGACGGGCAGACTGCACCGAGGCCCGGCCCTGTCATCGGCTGTCCTTACCTCACCGGCCCCGTGTCAGGTCTCACCCCGCCCCTCT GTGTCCGCGCACCCCACCTGACTGACCCCGCATCCCCCGGCTGCACACACACCCGTCCTCCCACTCACCCGTCCTCCCCACTCACCCGTCCTCCCACTG ACCCGTCCCCCCACTCACCCGTCCTCCCCACTCACCCGTCCTCCCAGGGCTCTCCCGCAGTCTCCTCGGCCACCAGGATGTCGTACTCCTCGGCCGCGT ACTTCTCCCAGTCCGAGAGCTCGGGGCCGCCGCTGTCACCGTCCTGGAGGAAGCACACGGGTTACGTACACGGCGTGGCGCGGCCCGGCCCGCGTGCCC GCCGCACTCACCCTGAGCAGGGAGATCTGCTCTTTCTGCTCGTGGTCGAGGTACTTCTCGATGTTGAAGAAGGTGTCGAAGAAGACGTTAGCCAGCTTG CAGCGCTTCAGGTCCTGCAGCGTGATCTTCCCTGCGGGGAGGGGAGTGCGTCCAAGGCGCGTGAGCCCGGTCTCACCTTCGGGGCCTGGGTGTGGGGTG CGCGCGTCCTGTCACACGTGCTTAAGGACGCGGCACGAAGCTCCGGGCTCTCACGCCACGTGTTTGACGTGAAAGGCGGACACGCGAGTGTCCTGGTTC TGGGTGGGGACTGAGGCGTGGGAGGCCGTGATGCGGAGGCGACTAGGGGCCACCGGTGCCCCAAGGATGCGTGGACTGGGACTAGCGCAGGGAGGCGCG GCCAGCAGGGGCGCTGCTGGATCTGAAATTACAGAGAACGGAGCTCCCCGTGCAGGCAGAGGCCAGAGGGTGTCCCGGGGACACTGAGCAGCAGGAGGC GCCCCATGGCCGGGGGGGGCAGGGCTGCAGGGCGGTGCCCGGGCCTCAGAGCCATTTCCAAAGTGAAAGTCCCAGAGCTGGATTCTGAGATGCAGAAGG AAGCCAGGCGGCGTGGACGGTGGGGCTCCCCTTTGCCTTAGAGGGCACGGGGGTGGGTGGGAGGAGCTGGGGAGATGGTGTGGGGGGAACAGGGGTGAG GGGAGGAGCCGGGGGGGGGTGAGAGGGGAGGAGCCGGGGGGGTGAGAGGGGAGGAGCCGGGGGGGGTGAGAGGGGAGGAGCCGGGGGGGGGTGAGAGGG GAGGAGCCGGGGGGGGTGAGAGGGGAGGAGCCGGGGGGGGATGAGAGGGGAGGAGCCGGGGGGGTGAGAGGGGAGGAGCCGGGGGGGTGAGAGGGGAGG AGCCGGGGGGGGGTGAGAGGGGAGGAGCCGGGGGGGGGTGAGAGGGGAGGAGCCGGGGGGGGGGTGAGAGGGGAGGAGCCGGGGGGGGTGAGAGGGGAG GAGCCGGGGGGGGTGAGAGGGGAGGAGCCGGGGGGGGTGAGAAGGGAGGAGCCGGAGGGGGTGAGAGGGGAGGAGCCGGGGGGGGGTGAGAGGGGAGGA GCCGGGGGGGGGTGAGAGGGGAGGAGCCGGGGGGGGGTGAGAGGGGAGGAGCTGGGGGGGGTGAGAGGGGAGGAGCCGGGGGGGTGAGAGGGGAGGAGC CGGGGGGGTGAGAGGGGAGGAGCCGGGGGGGTGAGAGGGGAGGAGATGGGGGGGGTGAGAGGGGAGGAGATGGGGGGGGTGAGAGGGGAGGAGATGGGG GGGGTGAGAGGGGAGGAGATGGGGGGGTGAGAGGGGAGGAGATGGGGGGGGTGAGAGGGGAGGAGATGGGGGGGGCGAGAGGGGGGGAGGAGCCGGGGG GGGTGAGAAGGGAGGAGACGGGGGGGGCGGGGTGAGAGGGGAGGAGACGGGGGGGGGGGGGGTGAGGGGAGGACCGCCTTGTGCGGCATCAGCACGAAC GTGGCAACTGCTCCCTCAGCCCAGTGCCAACGTCCCCGGTGAGCTCCGGCCCTAGGTTCTCTAGGTCACCCCTCGGGGGGTGTCGACGCCCCGAATAGG AGGTTCTTGCACTGTGAGACTAGGCACCTGTGGTGCTGGCCCTCCTCACAAACTCAGTAGGACAGCGCCTCACAGGGACGTCCCCTCACCCTGGTCCGT CCCCCCTCCCGTCTGTCCCCTCACCCTGGGCCGTCCCCCCTCCCGTCCGTCCCCTCACCCTGGGCCGTCCCCCCTCCCGTCCGTCCCCTCACCCTGGGC CGTCCCCCCTCCCGTCCGTCCCCTCACCCTGGGCCGTCCTCCCTCCCGTCCGTCCCCTCTCCCTGGGCCGTCCTCCCTCCCGTCCGTCCCCTCTCCCTG GGCTGTCATCCGTCCCCTCTCCCTGGGCCGTCCTCTCGCCCGTCCGTCCCCTCACCCTGGGCCGTCCTCTCGCCCGTCCGTCCCCTCACCCTGGGCCAT GCCCTCACGGGGCATCACCTTCAGTCCTCGGCTTGACCAGGTCCAGCATCTGGCAGAGGCAGTCCTGGAAGGGCAGGGCCTCGATGGCCATGCTGTCCA GCCTTCGGCACTGCTCCTCGTAGAAGTACTCGAGCTCGAACATGGACAGGGCGCCGTCCCCGTCCAGGTCCATGCAGCGGAACCAGTACTCGATGCTGC GGCACGGCGAGCTCTGTCAGCCCCTGCCCTGGGCCCTCCCAGCCCGTGACCTGCAGCCCCTGAGGCAGCCCCCACCGGGGGTGCACGCGTCCCCGCTGT GCCTTGCAGCCCCCACCGGGCGTGCAGGCATCCCCTGCCCCCTGCCGCCCCCACCGGGCGCGCACGCGTCCCCCTGTGCCGTGCAGCCCCCACCAGGCG TGCACATGTCCCCCTGTGCCGTGCAGCCCCCACCGGGCGTGCACATGTCCCCCTGTGCCGTGCAGCCCCCACCAGGCGTGCAGGCATCCGCCTGGGGAC ACATGTCACATGGGCGGCTCCCGGCCCCTCCACTGGGACAAACGCATGCCGCAGCAGGAACCCACCTGGTCGGTGTTTTTTTGTCTTCCTCAGAGATCA AAAACCAGACAAAGTCGGCATAGCTGATCTTCCCTTCCTTCTGCACTTTTCTGCCTCTAGATCGAAAGCCAGGATGGAGAGACGAAGATGCATGTCAGG GAGAGCTTCACAGGAACGGAGCCCCTGTCCACGCGCCTCGGTGAGGGGAGCCCCCCGGGCCCGGCCCTCCTCCTGCCCCCCTCCTGCCCCTCCTCCTGC CTCTCCGGGGAGGAGGTGGAGGCCCCGTGGCCAGAGGGTTTTCCCCAGATCCAGGCAGGGTCAGGAGTGCACCTTCGTTACTGCTCACTCAGGCCCAGC GCCCGACAAGAACCCCCGACCTGGGGCCTGGGCCACCCCCTTCCTCAGACTTCGCGTGACAGTCTTGTGCCACCCCCCCCCACTAGGGATTCACGTGAC AGAGACACGTGCCCCCCTCGCCAGGGCCTGGGGTGACAACCACTCGCTGTCGGGGCACAAAAAGCTCACGTCAGGCAACGATGAGGAGAGGGACCGGGG TCCTCGCAGGGGCAATGGCTGCCGTCAGGCGCCTGAGCCGTACGTACCGTGTGACTGCTCCTGAGAAGATCCTGTCTATCATCTTGGTAGAAAGGGCTG GAAAGGAATGCGGTTGATGGGCAGCCCGCACCGTGCCTCGGCCCCGACGTCACCACCCCCCGGAGCCGAGACTGGATGCGGTGGGGACCGAAAAGCTGA GAGGACGCCTGGGTCTGGGAGAGCCCCGGGGCCCCGATGCCCCTGCACGGCCCATCCTAGGGGCCCACCACGCTTTCCCGTCGAGCAGAGCCAAGTCCA GCATGAAATCCACAGAGCGCAAAGCTGACCGCGGCTCCAAGACCGACTTGTAAAGAGCAGAATATTCAGGCCTCAAAGGTACAGCTTTCAGACGGAGAG AGAGACCTCGAGTGTGATCACGGAAACAAACACGTTTCAACCAAAGGTTCACCAACGGGAGACGGGAGTGAGACCTCAGCAACGGGAGGCGGGAGTGAG ACCTCAGCAACGGGAGGCGGGAGTGAGACCTCAGCAACGGGAGGCGGGAGTGAGACCTCAGCAACGGGAGGCGGGAGTGAGACCTCAGCAACGGGAGGC GGGAGGGAGACCTCAGCAACGGGAGGCGGGAGGGAGACCTCAGCAACGGGAGGCGGGAGGGAGACCTCGCCAACGGGAGGCGGGAGGGAGACCTCGCCA ACGGGAGGCGGGAGGGAGACCTCGCCAACGGGAGGCGGGAGTGAGACCTCGCCAACGGGAGGCGGGAGTGAGACCTCGCCAACGGGAGGCGGGAGTGAG ACCTCGCCAACGGGAGGCGGGAGTGAGACCTCGCCAACGGGAGGCGGGAGTGAGACCTCGCCAACGGGAGGCGGGAGTGAGACCTCGCCAACGGGAGGC GGGAGTGAGACCTCGCCAACGGGAGGCGGGAGTGAGACCTCGCCAACGGGAGGCGGGAGGGAGACCTCAGCAACGGGAGGCGGGAGGGAGACCTCAGCA ACGGGAGGCGGGAGGGAGACCTCAGCAACGGGAGGCGGGAGGGAGACCTCAGCAACGGGAGGCGGGAGGGAGACCTCAGCAACGGGAGGCGGGAGGGAG ACCTCGCCAAGGAGAGGCGGGAGTGAGACCTCGCCAACGGGAGGCGGGAGTGAGACCTCGCCAACGGGAGGCGGGAGTGAGACCTCAGCAACGGGAGGC GGGAGTGAGACCTCAGCAACGGGAGGCGGGAGTGAGACCTCGCCAAGGAGAGGCGGGAGTGAGACCTCGCCAACGGGAGGCGGGAGGGAGACCTCGCCA ACGGGAGGCGGGAGGGAGACCTCGCCAACGGGAGGCGGGAGGGAGACCTCGCCAACGGGAGGCGGGAGGGAGACCTCGCCAACGGGAGGCGGGAGGGAG ACCTCGCCAACGGGAGGCGGGAGGGAGACCTCGCCAACGGGAGGCGGGAGGGAGACCTCGCCAACGGGAGGCGGGAGGGAGACCTCGCCAACGGGAGGC GGGAGGGAGACCTCGCCAACGGGAGGCGGGAGGGAGACCTCGCCAACGGGAGGCGGGAGGGAGACCTCGCCAACGGGAGGCGGGAGGGAGACCTCGCCA ACGGGAGGCGGGAGGGAGACCTCGCCAACGGGAGGCGGGAGGGAGACCTCGCCAACGGGAGGCGGGAGGGAGACCTCGCCAACGGGAGGCGGGAGGGAG ACCTCGCCAACGGGAGGCGGGAGTGAGACCTCGCCAACGGGAGGCGGGAGTGAGACCTCGCCAACGGGAGGCGGGAGTGAGACCTCGCCAACGGGAGGC GGGAGTGAGACCTCGCCAACGGGAGGCGGGAGTGAGACCTCGCCAACGGGAGGCGGGAGGGAGACCTCGCCAACGGGAGGCGGGAGTGAGACCTCAGCA ACGGGAGGCGGGAGTGAGACCTCACCAAGGAGACGCGGGAGTGAGACCTCAGCAACGGGAGGGGGGGAGGGAGACCTCACCAAGGAGACGCGGGAGTGA GACCTCAGCAACGGGAGGCGGTAGGGAGACCTCACCAAGGAGACGCGGGAGTGAGACCTCAGCAACGGGAGGCGGGAGGGAGACCTCACCAAGGAGAGG CGGGAGGGAGACCTCAGCAACGGGAGGCGGGAGGGAGACCTCAGCAACGGGAGGCGGGAGGGAGACCTCAGCAACGGGAGGCGGGAGGGAGACGTCGCC AAGGAGAGGCGGGAGGGAGACGTCGCCAACGGGAGGCGGGAGGGAGACGTCGCCAACGGGAGGCGGGAGGGAGACCTCACCAACGGGAGGCGGGAGTGA GACCTCACCAACGGGAGGCGGGAGGGAGACCTCAGCAACGGGAGGCGGGAGGGAGACCTCACCAACGGGAGGCGGGAGTGAGACCTCAGCAACGGGAGG CGGGATTGAGACCTCACCAACGGGAGGCCGGAGTGAGACCTCACCAAGGAGAGGCGGGAGTGAGACCTCACCAACGGGAGGCCGGAGTGAGACCTCACC AACGGGAGGCGGGAGGGAGACCTCACCAACGGGAGGCAGGAGTGAAAGCACCGTCGCCGTCAGCTTGGGCCACGAGAAGGTCCCGCAGCCTGGGCGGCC ATCCCTGCGGTCACCGGTGTCCCTGGGACGCACGAGCCAAGGTGCCGCCCCCCGCTTCAGGCCGCAGTGCGTGAGAAACAGCGCAGCCCGGCCGCACAC GGCATCCTGCCCTGGGACCGAGAGTGGGCTCCAGAGGAACGCGGAAAGCTGGGGCCGCGCCGGGCCGGGGGTTCACAGGGACAGTGAGCGCCTGGTTCT GGGCCGATTCCCAGCTGTGCAGGTGCTGGCCCAGGCCACACGGGCCGGCGCTGTGGAGCTGTGCTCTCCTCGTGGCTAGTTAGGGGCCCAGGGCGCAGG TGGCTCCCTGAACTGAGCGCAGGACAAATCCAGGGAATGCTACGTCTGCTCAGGTGCGCGTCCGACGCAGGGATCTACTGCGGCCACGCGACCCAGGAC CAGCCCCGGGAGACGCAGACGGGTGCAGCTCACGTTCGGGGAACCAGGCGTATTATGAGTCGACCACACACAACAGATGCCACCGTAATTCGGAAACAG TCTTGGTCATATGGTTTTTTGGGGTAAAAAGCACACTTAAAGGAATCCTTTAGAAAACTCAAGCCCCACAGGCCACAGGCCGCTTTGAGTTCCTGCAAG TGTGGATGACTCTTAGAGGGGCCGGGAGGCAGTGGCTGCTGTGAGGACACCTGGGCGGCACCGGCTCCCGCGGAGGTATATGAACGACCGCTAAGCCTG GGGCTCCACCTAGCCCGGGATTGGATGGAAACTGCTCCAGGCCTTGGGGGCTGGAACCTGGAGCTCCTGAGGGAAGGCGTGTGGCCTGCCCGCCCTTGC TCGGGTGTTAACAAGGAAGCCCCACAGCGCTGCTGGCCCTCGGGCCAGGAGCTTCAGGACCAGCGGCCCACACTGACCCTGTAGACGCCCCCAGGCAGA GGCCTCGGGCAGAGGCGCACGCGGGGACCCAGACACGGGGCAGCGACTGGGGAAGGAGAGGCAGCTGCAGACACAGAGCGGCGAGTGCGAAGGAGAGGC AGCTGCAGACACAGAGCTGGGAGTGCGGAAGGAGAGGCAGCTGCAGACCCGCAGGCCCTGAGAGAAGGGTGTGCTCGGTGTCCGCGCGGCCCGCCCGCC CCTGTGCCCCCACGCACCGTGGTCATTGTGCCGCGCCAGGTCGTCCGCGTCGATGAGCAGGTCGTGGTCCGTGTCCAGCTCCCAGAACTTGCAGTAGAT GACGTAGAAATGCTCGTACGAGAAGAATTCGGTCAGCTGGTTGATGTCCGCCTCCTCCTCCAGCAGCGCCACATTCTGCCAAAGGACCCAGGCGGCCTG AGCGCGGGGCCTCTGCGGGGATGCCCCAAGTCCGCCTGGCTGCGGGCGGGGCAGGGAAGGCTGGGAGGGTCGGGGCCGCTCCGTGGGTGGGGGGGACTG GGCAGCTGGGGCCGGGGGGCACTCCGAGTGGGGGCAACTCTGCGGTGGGGGGAGCTCCAGCATGGGGGTGGCTCCCTAGGCGGGTGTGGGGGTGGGGGC AGCTCTGGGGGAGTCAGGGCTCGGCTCCCCCTCAGGGTGGTGCCCAGTACGGCCCCACCCGCCTCCTAGATCAACCGTGGACCCCCAACCGCAGGCGGC GGGGTTCCAGTGCTCACAGGCCTGGGGGTGGGCTGGGAGCGCGGTGGAGGTGGGGGTGGGGGTGGGGGTGGGAGAGGGGGTGGGAGGGGAGGAGGGAGG GGGGAGGAGGGAAGGGAAGGGAGTGGAGGTAGGAGGGGTAGGGACAAGGCAGGGGGCAGGGGACAGGGGGCCGCTCCGCACCTGCAGGAAGGAGCTCCT CCGCAGCTCGGCGCAGGTGATCCTGCCGGACCAGGACCGGTTCACGGCGTAGAAGATCCGCTGGATGACCTGCGGGGGCGCTGTCAGTGCGGTGGGTGC GCAGAGACCCCCAGGAGCCTCGCCCCGCACGGAGACCGGGAGCCGGGAGAGGGGCGCGCCCTTGGTCTCAGCCGCACGGGGCCGCCAGGGCACAGGCGG GGGCAGAGGGAAGGGCCCTGCGGGAGGCGCCGCCCCAGGCCGCGGAAAGCGGGGAGGGTCTGGCCGGGGACGCCCCGGAGCTACACGGGCCCAGGACAG GTGGGAAGGGGGTGCGGCCACCCCGGAAAACCAGAGTCCCCCCAACACCGGGCTCCCGGGCGGGTGGAGACTCTCCCAGAGCGCGGCCGCCTCCTCCGG AAGCTCAGGAACCCCGGGCCCCGCCCGCCCCGTCCGCAGAAGCCCCGCGGCGGCCGCTGCAGAAACACCCGCGCCCCGCGCTGGAACCGACGGCCCCTC CGCTGGGGACCCACCGTGGTGATGTAGCGCGAGTGGAACTCGGACGCCTCCTTCAGGAACGACAGCCCCGGGTGCGTGTTCACCACGTCCTGCGGGTGG GAAGACACGAGGCGCGTGGTGTAGACGCCGGCCCTCCCGTGAGGTGTGCGGTGTGGACGCTGCAGACGCGGACCCTCCCGTGAGCGATGAGGTGTGCGG TGTAGACGCCGGCCCTCCCGTGAGGGATGAGGCGTATGGTGTAGACGCCGGCCCTCCCGTGAGGGATGAGGCATGTGGTGTAGACGCGGGCCCTCCCGT GAGGTGTGCAGTGTAGACGCGGACCCTCCCGTGAGCGATGAGGTGTGCGGTGTAGACGCGGACCCTCCCATGAGGGATGAGGCATGTGGTGTAGACGCG GGCCCTCCCATGAGGTGTGCGGTGTAGACGCGGACCCTCCCGTGAGGGATGAGGCGTGTGGTGTAGACGCCGGCCCTCCCATGAGGCATGCGGTGTAGA CGCGGGCCCTCCCGTGAAGGATAAGGCCTGTGGTGTAGACGCAGGCTCTCACCTGCAAGAAGGGGACAAAGTCCTCCTGCACCAGGTAGTTGCAGCCGG GGCTCATGAGCAGATGGACGAACTTGGCCGCGTCGTCGTGGCAGTTCTGGAGGATTCTGGAAGGACAGGATGACTGGGCACCACCCTCACAGGGGGGTG GCTTTCGACCCCGCAGACGCAGGGTGGAACACGTGTGTGGTGTTCCACGTGGTGCGTGGCAGGTGGCCACACACGACGGCCAGGCCTGTGCCCGTACAA GGGTTTCTCCTCTCACTGCGGCAGCCTCAGGGGGCACCCGTCCTGGCACCACGGGGACCCGGGGACGCCTCCGCCCTCCCGACACAGCCCCCTGCCCAG CCCAGGACTCACTTTCTCCACATGGCGACGAACTTGTGGACGGACACGGAGCCCGTGCGCTCCCCGCCGGCGCCATAGAAGAGCGGCCCCTTCCAGTAG AGGGGGCAGCCGCAGGCCTGGGGCAGAGAGGGCAGGAGTGGGCAGTCAGCAGGGCCTGGACGCCGGCGCTCCTGCTGCGTACCTCGCGCCTGACCGACG CCGCCCAGAGACCCTCTGCTGCAGAAAGACACAGCACGCTCAGCGCGGCCTGTCTGGGCATCTGCAAACTCAACGTGGCTTTCGCTCCAGCCTTCAATC AGTCATGAGAGCTGAGCGTGGGAGTGCCGGGCACGCAGTATCCCCTGACCACAGACCACGCGTGGGCACTGTAACCAGAGGCGGCTGCATCCCCAGCGT CTGGAAATCAAGCGGCACGTACTAAGAACCAGTAAGTACGGGAATAAATGGCCCCGTCAGCCAGGCAACGCGGTGAATCGGACGGCGACAGAAACATCT CATGGAATGTGTGGGACGCACCTACAGCAGCGACCCATGGCAGGTTCACAGCCTTAAATACACGCGTGAGGAAGGAAAGTTTAAAATCGATCGTCTACA CTTCTACCCCAAAGGAACAAATACATGGCAGAGATGGAACGCAAAGTAAATAGTAACAGAAAATCAACAAAATCAAAACTCGATGATTTGAAAGATTTC TTAAAAATTAATTAACCCTGGCTGGCTGGGCGTGGTGGCTCATGCCTGTAATCCCAGCACTTTAGGAGGCCGAGGGGGGTGGATCACCTGAGGTCAGGA GTTCGAGACCAGCCTGGCCAACATGGTGAAACCTCGTCTCTACTAAAAACACAAAAATTAGCCTGGCATGGTGGCGGGCGCCTGTAATCCCAGCTACTC GGGAGGCTGAGGCAGGAGAATCACTTGATCCTGGGAGGTGGAGGTTGCAGTAAGCCACGATCACACCACTGCACTCCAGCCTGGGTGACAGAGAGAGAC TCTGTCTCAAAAAAAGAGCAAATACAGATTAGCCATGTCAGCTGTAACTACAGATCCTACAAACATCAGATAGTGGATGCTCATGAATCACTTTATGCC TGTAAGTCAGATGACTGCTGAAACAGACTCCCCGATGAACAAGTCAAAAAATAAACTACAATAGAAAATCTGAAAGAGCTGTTTCTGCAAGAAATTGTG TCCATAATTTAAAACTGTCCCACCAAGAAAATTCCAGCCCCCAAAGGCTGCTCCCATGAGTCGACCAAACATTGAAGGAAGAAATCACACATGCGTTCC CCTGGAGAAGAAAAACGAGGGGCGTTTCCCAGTGCCTGTTCCGAGGCTGGCACAGCCTGGACGCGGTCAGGGACACAGGAAGTCACAGGCCAGTTATCA CTGGAGAGCAGAGGCAGAAATCCCACACAGAACACACAGTAGCAAGTCCAACTCGGCAATAATAGAACAGGAATATCTAATAATAATAATAGACCAGGA AACCAGAATCAGGAGGGACACTCTTATTCTCATAAAGGGTGCTATGGCCTGAATGCCTGGGTCCCCAAGATCCACGTGCTGAAATCCTCTCCCCCGAGG CGATGGTGTTGGGAGGTGGGGCCTCATGAATGCGATGAGTCCCCTTCTGAGGGACCCCAGAGAGCTCCCTCACCCCTTCCACCCCGTGAGGACGCAGCG GGAAGGCGCCGTCGATGAACCAGGCACCACTCTGCCACACCGAGATCTTCCAGCCTGCAGACCTGTGAACAGTAAATGTCTTGCTGGTGACAAGCTGCC CGGGCTATGGTGTTTCTTGACAGCAGCCTGAATGCTCAGACAAAGGGCCTCTACGAAACACCCCAGCTGCTCAGACAGCGATGACACATGAACACTTCT TCCCTGTGCTGGGAACAAGAGGACAGTGTCACCACCAAGGCCGCCTGGACGGCCGAGCCAGGGCAGCAGGCAAGGGAAAGACCGAAAGATACGACGACG GGTAAGAAGAGGCAGAACCGTCATGATTCACAAGCAACACACTGTGTAGACAATCCAAATGCTTACCAAATACGGGAATTAATAAGTATCTTCAGCAAA GTTGCTGGATCCAAGGACTATATGCAAAAATAAATTATTTCAATATACTGGCAACAAACAACTAAAAATAAAATCTTCAATAATGCCATCTATCAAGAG CATGAAAAATCAACCTACGAAAAATGGAACAAAAATCACACACAGTGTCTACAAAGCAAACTGCAGGGCACGGCTGGGTGAAATCAAAGACGACCTCAA CGTGGTGAGGTGGACCTCACTGCTGAGTTCACAGACTGCAGTTTCCTCCAGACGACTCTGTTGACCCAGAGTCCACGCGATCCCCACCCACAGAGTCCA GCAATCCCCATCCACATGCACCGAGGGCCTGTTGTTTTGGGGTTTGTAGTATACACGGACAGGCTCATGTGAAAACTTACCAGGGAACACAAAAGAACT CACAGGGGGACCAAGTTGGTGGATATGAGTCCCACTGCCCGGTTTCAACCCTTCCTGTAAAGTCAGAGTAATGAAGACTCAGTCAGTCTCTGTCGGGCA CCGGTGGCCTAAGAGAGCAAGGGAAGGAAGTGAGTTGGGACAGAGCCCCGTGGAATATCCACAGGCAAAGACACTTCACACAAACTGAATACCAGGGGA TCCCAGCCCGAAGGGTGAAAGGCCAAACAGCGGCCCCTCCTGGAAGGAAACGAGGGACGCTGCACGGCTCTGCCGCGGCCAAGGTTTCTTTCACGGGCC ACACGTGCGCTCCCTGAAGGAAGGGAGCACCGCTGCACCGCCCCAGCGCAGGACTCAGGGTCAGCTACCGGCACGGTGAACACCATGACAGGACATGAC ACAGAGGGAGAAATGTCTCTGCAAGGCGAGATTCTGACGAGGGACTCAGAGGCAGAATTTTAAGCATCAGTGAGAAAGAGGCCCGAACACGACCGAGTG GACATGCACTGCCCTCCGGAGAGGGGTGCACGGTGGCCGTAGACAAGGGGAAGCGCCCAGCACTGGCGCCGGCGAGGGCAGGGTTCACAGGGCATGGAG AGGTGGCAACAAGAACATGACACGCGCGGCTGAGACAAAAAGGACTGAAGACGCCAGGTCCCGCGAGGCTGCCAGGCAGCTGGGTGTCCTCCGCGTTGC TGGGGGAACTGGGACGCCACGCAGCTGCCGGGAAAGCGGCCCACGGGGCGGCTGAGCGTAAAGCCCCGTGGCCCGGCCAGGACATCTGTCCTCCCAGAA GCACACCAGTGGGAAGAGGGCCCGTGCCCGTGAGAGCCTGCACAGATGGGGACGTCGGCTGCTTGGTTCACGGTGCCCCAGGCGAGAGGAGCCGGACGC CAGAGCAGGGACTGGAGGAATAAACTGTGGCAGAGTCGTCCTGACAGGTGCAGGGCGGGAGTGACCACGGGGGGGCGTGGGGGACTGCAGAGGGGGCTG CGCGCCACAGCGGGGATTGCAGTGCTGCTATGGGAAAGGACGCGCTCCGGCTGGCGCACCTGTGGATGACTTGAAGGCAGCCGTGTGCGGCTGGAGGGG CCGGGCCCCGCAGGACGCACAGGCACGTGCCGGCTCCCGTGATGAGGATCCTGACGGAGCTCAGCCCTGGCCACCTGGAAGGGCGTCCCGGTGCGGTGG GAGGGAACGAGCCGGGTGCAGCACCGGGTCCTTGGGCTAGTGCTAGTCCCACGCTTTTGACCTCATGAGACCATTCTGGCTCACAGGCCGTGCAGGTTT CAAACCCCAAGCGCTCAACACCCAGGGATGGGGGGATGTGTGAGCCTTCACATCTGTGCTCCTCACCGCGTGCAAGACACACCGTCATGACCCGGTGGC AGGGATGGAGCATCAGATCTATCCAGCCGTGCAGGATATAGCGTCTCATGTGTCCAGACCTGCAGGCTGGAGTGCAGTGTGTGATCACAGCGTGATCCA TTTTTATTCTACTTGTTTTTCGAGACACGGTCTTGCTGTTGCCCAGGCTGGAGTGCAGTGTGTGATCACAGCGTGATCCATTTTTATTCTACTTGTTTT TCGAGACACGGTCTTGCTGTTGCCCAGGCTGGAGTGCAGTGTGTGATCACAGCGTGATCCATTTTTATTCTACTTGTTTTTCGAGACACGGTCTTGCTG TTGCCCAGGCTGGAGTGCAGTGTGTGATCACAGCTCAGTGCAGTCTCAACTTCACGGGCTCAAGCAATCCTCCCACCTTAGCCTCCCAAGTAGCTGGGA CCACAGGTGCGCGCTATCATGCCTGGGTAATTTTTTTAATTTCTACAGTGAAGTCTCACTATGTTGCCGAGGCTGGTCTGGAACCCTTGGCCGCAAGCC ATCCTCCCGCCTTGGCCTCTCAAAGTGCTGGGACTACAGGGATGACACTGCAACCAGAGACAGCTGCACCCCCGGCGTCTGGGGGATGAGAACTGAGCG GGAGAGTGCTGGGCACACAGTCCCCCCACCACACCAAACCCGAAAATTACCTCCAAATGAATTAGCTCATAATTTAAAATAATCCTAAAGTAAATGGAG ATAATCAACTCTAAAATGCAAAAGATACAAAGGAAAGCAGTTTTACCTGAATGTCACAAAATCCTTATGGGAAAGGGAATAAAACATCAGATACCCTGA TCTGACTTTTAAAAGTCTTTTCGTGATCGACACACAGAAAGACCGACTCCTTGTGGGGCCCGGCAACGCGGCGGCACGCCCACCCGCACGGCCAGGACA GGGAACGAGGCCGCCAGCCCTGACAGTCCCTTCGGCCCCTCCGCAGTCCGCTACGGCGACGGCCCCCAGGCCTCCTCCCCGTGGCTCCAGTTCACCTTT TCCAGGACACGGCAACGGGACCCGGCCATCTGCGGACTTTTAGACCTGGCTTCCCTCGCTCAGCACATGCGTGTGGGACTCACACACCTTGCTCTGTGT GGATCGTCTGCCCACCTTGCTCTGTGTGGATCGTCTGCCTGTGTACTGCTGAACCCTATTCCATTATGGCTCTGCTGACCTCAGAGTAGCTCCCTGAGC AGGAAACACCGCCGGCCCCAAGGCCCACGTGATGGTGCTGCTGAGGGGGACTCGCGAGGCAGACAAAGTCCCGAGCGTCTACAGTCGCACAGGGCACGC CGACACCAACCGACAGGTGTGAGAGGAGAAAGGAGGACGCCACAACCACGGCCGGCAAGTTCAGCACGCGTTCCTCGGCGGGTGACGGGACAAGAGGAC GATCTGCAGACACGTAAAGATGTGAAAACGCTGCCAAGCTATGTGAGCGAGTGACATCGCGGAGCCGGAGGAGTCTTCAGGGTCACATGAACCAGTGGT GGGCTGTGGCTCGGAGCCCCCACCCAAGTCTCACGGGGAACTGTGATCCTGAGTGTTGGAGCAGGGCTCTGCTGGGGGGTGACTGGGTGATGAGGGTGG ACAGCCCCCTTGCTGGTCTCGTAATCCTGAGTTCTTGTGAGATCCGATGGTCTAAAAGTGGGTGGCAGTGGCCGGGTGCGGTGGCTCAGGCCTGTCATC CCAGCACTTTGAGACGCCAAGGCGGGTGGATCACCCGAGGTCAAGAGTTCGAGACCAGCATGGCCAACATGGTGAAACCCCATCTCTCCTAAAAATACA CAAATTAGCCGGGTGTGGTGGCGCATGCCTGTAATCCCAGCTACCCAGGAGGCTGAGGCAGGAGAATGGCTTGAACCCAGGAGGCGGAGGTTGCAGTGA GGTGAGATGGTGCCATTGCACTCCAGCTTGGGTGACAGAGCAAGACTCTGTCTCCAAAAAAAAAAGAGAAAAGATGATACAGACAGCCTGTGTTTATTG AAACAATTCATGCTGTCATTAAAAACCTTCCCACAGAGAAAACTATCCCAAATGGCTTCACTGATGAGTTCTACCAAACATTCAAATAAAAATAACACC CATTCTAAACAAATGGCTCCAGAAAAGTGAATTCTAAAGGACATTTTCCAAGCAACTCTCCGAGGCCAGCATCCCCCCGATGCTAAACCAGACCAAGCT ATCACAAGAAAAGGAGACCGCAGGCCAGTCCTTCACGAGCGCAGACGCAACCCCTGCGAGGCCTGGCAATGTGGACCCAACACATAGGAGAAGTGTGAC ACTGTGTGGTGGACCAGGGACCGGGGGCTGGGGGCTCACCCAAAGACCGGGGGCTCACCCAAAGACCGGGGCTCACCCAAAGACTGGGGCTCACCCAAG GAGCAGGGGCTCGCCCAAAGACCGGGGCTCGCCCAGGGACCGGGGGCTCACCCAAAGACCAGGGCTCACCCAAAGACCGGGGGCTCACCCAAAGACTGG GGCTCGCCCAGGGACCGGGGGCTCACCCAGGGAGCAGGGGCTCACCCAAAGACCAGGGCTCACCCAAAGACCAGGGCTCATCCAAAGACCGGGGCTCAC CCAGGGAGCAGGGGCTCGCCCAGGGACCAGGGGCTCACCCAAAGACCGGGGCTCGCCCAAAGACCGGGGCTCACCCAGGGACCAGGGGCTCGCCCAAAC ACAGGGGGCTCACCCAAAGACTGGGGGCTCACCCAAACACCGGGGGCTCACCCAGGGACTAGGGGCTCACCCAAACACCGGGGGCTCACCCAGGGACCG GGGGCTCACCCAGGGACCGGGGGCTCACCCAAACACTGGGGGCTCACCCAAACACCAGGGGCTCACCCAAACACCGGGGCTGCCTTCACACTCAGACGT CAGTCAGGTCAAGACCAGTCCTTCATGACCACAGACGCTACCCCTGCGTGGCCGGGCAATGCACGCTGCGGTGGAACAGGGACCGGGGGCTCACCCAAG GACCGGGGCTGCCTTCACACTCAGACGTCAGTCAGGTCCATTCACCTTTTACAGGACCAAAGGAAAACCTCACTGCCCTCTCAATACACGCAGAAATAC ACATTTAGCATCCCGGACAAACTTCAAGGCACTTTTATAATTTTAAAAAGTAAACTGAGGCCAGGCGTGATGGCTCATGCCTGTCATCATCCCAGCACC ATGAGAGGCTGAGGCAAGAGGATCCTTCCAGCCCAGGAGTTCCAGACCAGCCTGGGCAACATAGCAGAACCCTGTCTCTACCAAAAAAGAAAAAATCAG CCAGGTGTGGTGGCGGGTGCCAGTAGTCACGGCTACTCGGGAGGGCTGAGGTGGGTGGATCACTGGAGCCCAGGAGGTCGAGGCTGCAGTGAGCTGAGA TCATGCCACTGCACTCCAGCCCGGGGGCAGAATGAGAACCTGTCTCAAAAAAAACACAAGAGCTCCCAGCAAACAAGAAACCAAAAGGGAAGGGGCCTC CTGAGTCTGATGAAGGACGCCAGGCAGGGCCGCTGCGTCACGGACGACTTGAGCCCGAGGCGGGAAGGGGCGTGCTCTCCACGGCTCTCCAGCACTGCA CTGGCAGGCCGAGCCTGTGTACAGAAAGTCAGGGAGGAGACACCGATGCAGACGAGAAGAGAAAGGCTGAACTGTGCTCACCTGAAGATGACCTGACCG TGCGTTAGAGACTCTGCCGGACTTAAAGGTTGAGCTGGTAGAGCCGATGACTGAGTTAAACTTGGTCAGAAAATGCAAAGTCCACATATAAAACCTAAC AGTGCTTCTCAACATTCACAATTGACAATCATATTAAAGTAAAAAAATACCACGTACAGTGTCATCAGAAACAGGAAATGCTTGGGTATCAATGTGATG AACCGTGAACAAGACCAAACTACAAGGCGTACTGAAAGAAGTGAAAGATGCCTGAAATAAGGGAGAGCTGGACCGCGTTCATGAGACGGAAGCCTCAAT ATGGTCAAGATTCTTCCAAGTTCATCTGGAGTCAACAGGATGCCCATGTAATCCCAGCAGGCTGTCGTAGCAACGGAGGGACCGGCTCTAAAATTCACA CGGTGGGAACGAACAACGGTGCAGCCACTCGGGAAGACAGCGTGCAGGTTCCCCAGAAAGCCGGAAGTGGAACTGCACGCGCACACGGAACACCACGCG ATCCGGCCCTCCACACACGCACATGGAACACCGTGCTGTATATGCCACAAACGTCTCCGAGTTCTGTGCTCCAAATGGTTACGGTGGTGAATTCTGTTG TGTGAATTTTCCCATAATAAAAAAAAGTGCCTGCTGTCTGATTCTGTTTACATAAAATTCCAGAAAATTCCGGCTAATCTATATTGACAGAAAGGAGGC CTGAGGTAGCGTGGGATGAGGGCAGGTGCGCGTCAGAAAGCAGCAGAGAAAAGTCCAGCGCACGTGCCCGCTCAAGCGCAAGGATGCAGGGACGCCTAT TGTCTTGGTGCTGTGGATAGTTTCATGGGTGGGTACAGACGTCAAAGCTCATTGAATTGTACCTTCCACACGCACCATGCATCCCACATCAGTTATATC TCAACACAGCTGCCCCAAAATGAAAAAAAGGCAAACTGATGAGAAAGAAACACGTAAGGTGGTCAACACGTACATCAGGGAAATGAGGAGTGCAGCCAG GACGGGACGCCTGCACACGCACAGAACGGCTAAAGGCAAAGCGTGTGGCCACGTGCCCGCAGGGGTCAACGCTGCTGCCTGGAAATGCGTCACTCAGGG CCACCCCGGAAGCAGGGTTTTTTGTTTTTTGTTTTTTTGCGTAGAGACACAGTCTTGCTCAGTCGCCCAGGCTGGAGTGCAGTGGTGCGATCTTGGCTC ACTGCAACCTCCACCTCCCAGGTTCAAGCGATTTTCCTGTCTCAGCCTCCCGAGTAGCTGGAATGACAGGTGCCCGCCACCATACCTGGCTAATTTTTG TATTTTTAGTAGAGATGTGGTTTCATCATGTTGGCCAGGCTGGCCTCGAACTCCTGACCTCAGGTGATCTGCCTACCTCAGCCTCCCGAAGTGCTGGGA TTAGTGGAGTGAGCCACCACTCCTGGGCCCTGGAAGCAGTTTTATAGCTTCTTACAAAACTAAACATGTGGCCACCAGATGAGGCAGAGCTCACCTCTG CGGTATTAATCACCTCAGGAAAATGCAAGCATGTCTGCGTAAATCAGTGCACAGACACACAGCAGCTTTACTCACAAGAAGCAAAAACCAAAGCAACCA GACGTCTATCAACCCTGGAACAGGGGAGAGATGGAGCACACACACAGCAGAAGCTACTCAGTGATAGAAAAGAAACTGCCCATACGGCCAGGGACACAT AGTGAGCCCCACAGTATCCACACCCTGGCCAGCCACACGGGAGCCCCACGGTAACCACGCCTTGGCCAGCCACACAGCGAGCCCCACAGTATCCACACC CCGCCAGCCACACAGCGAGCCCCACGGTAACCACGCCTTCGCCAGCCACACAGCGAGCCCCACAGTATCCACACCACAGGACTTAATCAGTCCTCAACA CACACACATAGCGAAACATCTTGCTGTACCCTGTATACACAAATATTACTTGTCCATTCAAAATAAA ÚKOL 5 Analyzujte část genomu mnohobuněčného eukaryotického organismu také pomocí programu GENSCAN (http://genes.mit.edu/GENSCAN.html). Porovnejte s výsledky z úkolu 4.