Practical Course
Binding Analysis for Experimental Biologists
12. - 16. May 2014
PC lecture room 1.21
A2
IC113
code for registration in IS MU
Výzkumná skupina Molekulární komplexy chromatinu
Národní centrum pro výzkum biomolekul, Přírodovědecká fakulta
Masarykova univerzita
www.biokin.com/courses/bkeb/index.html
Lecturer Dr. Petr Kuzmic
The course is dedicated to general principles of data analysis that can be
used in all biological sciences. The course includes theoretical introduction
lectures and practical sessions. Practical sessions will be held in the
computer laboratory using the software DynaFit. Real data from published
literature will be analyzed.
Lectures
• Matching mathematical models to experimental data
• Confidence intervals for rate and equilibrium constants
• Experimental methods for the determination of equilibrium and rate constants
• Analysis of equilibrium binding data
• Enzyme catalysis and inhibition of enzymes
Practical PC/laptop exercises
• Determination of an equilibrium binding constant for protein-protein interactions
• Determination of rate constants for protein-ligand interactions
• Resolution of stoichiometry for protein-ligand interactions
• Selecting an appropriate mechanism for enzyme inhibition
Students are encouraged to bring their own experimental data.