TABLE 5 Characteristics ot Candida spp. most commonly encountered in the clinical laboratory* Growth: - Organism Microscopic morphology on cornmeal-Tween 80 agar at 25 C In Sabouraud broth With cycloheximlde at 25"C On SOA at 37"C Germ tubes Urease (25-C) C. albicans C dubimiensis Pseudohyphae with terminal cfilamy oospores; clusters of blastoconidia at septa NSG 0 C. tropicalis Blastoconidia anywhere along pseudohyphae Narrow surface film with bubbles ov 0 0 C parapsilosis Blastoconidia along curved pseudohyphae; giant mycelial eel's NSG 0 + 0 0 C lusilaniae Short chains of elongate blastoconidia along curved pseudohyphae NSG 0 + 0 0 C guiltiermondii Fairly short, fine pseudohyphae; clusters of blastoconidia at sepia NSG + + 0 0 C ketyr (C p&Budotroptcalts) Elongated blastoconidia resembling "togs in a stream* along pseudo-hypnae NSG ♦ 0 0 C rugosa Pseudohyphae with elongated blastoconidia. some in chains NSG 0 0 0 C zeytanotdes Pseudohypnae give featherlike appearance at low power Pellide (delayed) 0 0 C giabrata No pseudohyphae; cells small, terminal Duddmg NSG 0 - 0 0 C. krusai Pseudohyphae with cross-matchsttcks or treelike blastoconidia Wide Surface film up sides of tube 0 0 C. tipolytica Elongated blastoconidia in short chains along pseudohyphae Pellicle (delayed) 0 * ' fct I ■' ins SOA, Sabouraud Oestro*« agar; +, powttve. 0 negativa. W reaction may bt weak; V. strain variation; NSG. no Surface growth. 'fermeniston is demonstfsied by the production ol gas (acid ctoes not indicate fermentation) I 0 1 i s S I ! 1 galactose ssimi i 3 ation s 0 1 3 of. c 1 o c > X ,1 o c £ rr jl a 1 1 I S -* s I 3 Fe 7. 0 1 rm«n s e ! ation 1 c ! Galactose 5. Trehalose I -Q O Z-(J B V 0 • 0 0 0 0" 0 i o- 0 0 • 0 0 V II 0 pv 0 ♦ 0 +* 1 0 - 0 ♦ o 0 + - 0 *" 0 + 0 0 0 0 + 0 0 0 + 0 0 0 V 0 - 0 t 0 0 + 0 0 0 0 ov V 0 V + + + 0 ♦ ' 0 + ♦ ♦ + 0 0 *<• 0 *" 0 0 + 4 0 0 + 0 0 0 • 0 - ♦v 0 0 + 0 0 0 1 0 3 0 V 0 0 0 0 0 0 0 0 0 0 0 — 0 0 0 0 0" □ 0 0 + 0 c 0" 0 0 0 — 0 ov 0 + 0 0 0 0 0 0 0 0 0 + 0 0 + 0 0 0 0 *v 0 + c 0 0 0 0 0 0 a 0 0 0 0 + 0 0 0 0 0 0 0 0 0 V 0 0 0 0 0 0 0 0 0 0 1 0 0 a 0 0 122 IDENTIFICATION OF FUNGI IN CULTURE DETAILED DESCRIPTIONS