Document Name: Skupina 1 Plate Type: Standard Curve Vliv inhibitorů User: Student Document Information Operator: Administrator Run Date: Tuesday May 16 2017 11:52:56 Last Modified: Tuesday May 16 2017 13:38:28 Instrument Type: Applied Biosystems 7500 Real-Time PCR System Comments: SDS v1.4 Thermal Cycler Profile Stage Repetitions Temperature Time Ramp Rate 1 1 50.0 °C 2:00 100 2 1 95.0 °C 10:00 100 3 40 95.0 °C 0:15 100 60.0 °C 1:00 100 Standard 7500 Mode Data Collection : Stage 3 Step 2 PCR Volume: 25 µL Standard Curve Information Detector Name Slope Intercept R2 Number of Standards Number of Unknowns CCND1 -2.786213 40.003639 0.948232 16 9 GAPDH_VIC -2.924167 33.242157 0.982112 36 19 Well Sample Name Detector Task Ct Qty A1 CCND1 CCND1 Standard 18.4294 150 A1 CCND1 GAPDH_VIC Standard 25.8697 150 A2 CCND1 CCND1 Standard 21.5279 15 A2 CCND1 GAPDH_VIC Standard 28.7357 15 A3 CCND1 CCND1 Standard 24.3866 1.5 A3 CCND1 GAPDH_VIC Standard 31.5662 1.5 A4 CCND1 CCND1 Standard 27.7297 0.15 A4 CCND1 GAPDH_VIC Standard 34.9805 0.15 A5 CCND1 CCND1 Standard 30.1494 0.015 A5 CCND1 GAPDH_VIC Standard 38.6585 0.015 A6 CCND1 CCND1 Standard 31.3094 0.0015 A6 CCND1 GAPDH_VIC Standard 36.7398 0.0015 A7 FOS FOS Standard 18.3039 150 A7 FOS GAPDH_VIC Standard Undetermined 150 A8 FOS FOS Standard 21.4056 15 A8 FOS GAPDH_VIC Standard Undetermined 15 A9 FOS FOS Standard 24.2154 1.5 A9 FOS GAPDH_VIC Standard Undetermined 1.5 A10 FOS FOS Standard 27.7687 0.15 A10 FOS GAPDH_VIC Standard Undetermined 0.15 A11 FOS FOS Standard 31.0664 0.015 A11 FOS GAPDH_VIC Standard Undetermined 0.015 A12 FOS FOS Standard 34.2391 0.0015 A12 FOS GAPDH_VIC Standard Undetermined 0.0015 B1 CCND1 CCND1 Standard 18.4036 150 B1 CCND1 GAPDH_VIC Standard 25.8965 150 B2 CCND1 CCND1 Standard 21.4893 15 B2 CCND1 GAPDH_VIC Standard 28.6825 15 B3 CCND1 CCND1 Standard 24.4518 1.5 B3 CCND1 GAPDH_VIC Standard 31.6747 1.5 B4 CCND1 CCND1 Standard 27.9473 0.15 B4 CCND1 GAPDH_VIC Standard 35.1995 0.15 B5 CCND1 CCND1 Standard 30.2364 0.015 B5 CCND1 GAPDH_VIC Standard 38.2376 0.015 B6 CCND1 CCND1 Standard 31.4168 0.0015 B6 CCND1 GAPDH_VIC Standard Undetermined 0.0015 B7 FOS FOS Standard 18.653 150 B7 FOS GAPDH_VIC Standard 38.5237 150 B8 FOS FOS Standard 21.3685 15 B8 FOS GAPDH_VIC Standard Undetermined 15 B9 FOS FOS Standard 24.238 1.5 B9 FOS GAPDH_VIC Standard Undetermined 1.5 B10 FOS FOS Standard 27.7048 0.15 B10 FOS GAPDH_VIC Standard Undetermined 0.15 B11 FOS FOS Standard 31.0959 0.015 B11 FOS GAPDH_VIC Standard Undetermined 0.015 B12 FOS FOS Standard 34.6231 0.0015 B12 FOS GAPDH_VIC Standard Undetermined 0.0015 C1 CCND1 CCND1 Standard 18.6895 150 C1 CCND1 GAPDH_VIC Standard 26.1399 150 C2 CCND1 CCND1 Standard 21.5834 15 C2 CCND1 GAPDH_VIC Standard 28.343 15 C3 CCND1 CCND1 Standard 24.4589 1.5 C3 CCND1 GAPDH_VIC Standard 31.0661 1.5 C4 CCND1 CCND1 Standard 27.7295 0.15 C4 CCND1 GAPDH_VIC Standard 34.798 0.15 C5 CCND1 CCND1 Standard 30.3379 0.015 C5 CCND1 GAPDH_VIC Standard 38.0906 0.015 C6 CCND1 CCND1 Standard 31.5435 0.0015 C6 CCND1 GAPDH_VIC Standard Undetermined 0.0015 C7 FOS FOS Standard 18.7039 150 C7 FOS GAPDH_VIC Standard Undetermined 150 C8 FOS FOS Standard 21.366 15 C8 FOS GAPDH_VIC Standard Undetermined 15 C9 FOS FOS Standard 24.303 1.5 C9 FOS GAPDH_VIC Standard Undetermined 1.5 C10 FOS FOS Standard 28.0202 0.15 C10 FOS GAPDH_VIC Standard Undetermined 0.15 C11 FOS FOS Standard 31.0923 0.015 C11 FOS GAPDH_VIC Standard Undetermined 0.015 C12 FOS FOS Standard 33.5546 0.0015 C12 FOS GAPDH_VIC Standard Undetermined 0.0015 D1 20 % FBS CCND1 Unknown Undetermined D2 20 % FBS CCND1 Unknown Undetermined D3 20 % FBS CCND1 Unknown Undetermined D4 bez FBS CCND1 Unknown Undetermined D5 bez FBS CCND1 Unknown 28.2555 D6 bez FBS CCND1 Unknown 26.1166 D9 noRT CCND1 Unknown Undetermined D9 noRT GAPDH_VIC Unknown 32.3923 D10 noRT FOS Unknown Undetermined D10 noRT GAPDH_VIC Unknown Undetermined D11 NK CCND1 Unknown Undetermined D11 NK GAPDH_VIC Unknown 32.2859 D12 NK FOS Unknown Undetermined D12 NK GAPDH_VIC Unknown Undetermined E1 20 % FBS FOS Unknown 37.5476 E2 20 % FBS FOS Unknown 37.8208 E3 20 % FBS FOS Unknown 37.1939 E4 bez FBS FOS Unknown 36.0935 E5 bez FBS FOS Unknown 37.0759 E6 bez FBS FOS Unknown 37.6394 E9 noRT CCND1 Unknown - E9 noRT GAPDH_VIC Unknown - E10 noRT FOS Unknown - noRT a NK E10 noRT GAPDH_VIC Unknown - nenapipe- E11 NK CCND1 Unknown - továno v E11 NK GAPDH_VIC Unknown - duplikátu E12 NK FOS Unknown - E12 NK GAPDH_VIC Unknown - F1 20 % FBS GAPDH Unknown 16.6395 F2 20 % FBS GAPDH Unknown 16.6686 F3 20 % FBS GAPDH Unknown 16.6944 F4 bez FBS GAPDH Unknown 19.7386 F5 bez FBS GAPDH Unknown 19.8824 F6 bez FBS GAPDH Unknown 19.9571 G1 20 % FBS GAPDH_VIC Unknown 19.3746 G1 20 % FBS CCND1 Unknown 26.6831 G2 20 % FBS GAPDH_VIC Unknown 16.6382 G2 20 % FBS CCND1 Unknown 24.8887 G3 20 % FBS GAPDH_VIC Unknown 16.9457 G3 20 % FBS CCND1 Unknown 25.3738 G4 bez FBS GAPDH_VIC Unknown 21.2117 G4 bez FBS CCND1 Unknown 29.8633 G5 bez FBS GAPDH_VIC Unknown 21.1432 G5 bez FBS CCND1 Unknown 30.2967 G6 bez FBS GAPDH_VIC Unknown 21.3178 G6 bez FBS CCND1 Unknown 30.622 G7 1 ul EtOH CCND1 Unknown 31.6994 G7 1 ul EtOH GAPDH_VIC Unknown 17.962 G8 1 ul EtOH CCND1 Unknown Undetermined G8 1 ul EtOH GAPDH_VIC Unknown 16.2312 G9 1 ul EtOH CCND1 Unknown Undetermined G9 1 ul EtOH GAPDH_VIC Unknown 18.5049 G10 1 ul EtOH FOS Unknown Undetermined G10 1 ul EtOH GAPDH_VIC Unknown 20.4713 G11 1 ul EtOH FOS Unknown Undetermined G11 1 ul EtOH GAPDH_VIC Unknown Undetermined G12 1 ul EtOH FOS Unknown Undetermined G12 1 ul EtOH GAPDH_VIC Unknown 19.7808 H1 20 % FBS GAPDH_VIC Unknown 18.4237 H1 20 % FBS FOS Unknown Undetermined H2 20 % FBS GAPDH_VIC Unknown 19.1789 H2 20 % FBS FOS Unknown Undetermined H3 20 % FBS GAPDH_VIC Unknown 18.294 H3 20 % FBS FOS Unknown Undetermined H4 bez FBS GAPDH_VIC Unknown 16.5937 H4 bez FBS FOS Unknown 35.3634 H5 bez FBS GAPDH_VIC Unknown 21.1738 H5 bez FBS FOS Unknown Undetermined H6 bez FBS GAPDH_VIC Unknown 20.3871 H6 bez FBS FOS Unknown Undetermined H7 2 ul EtOH CCND1 Unknown Undetermined H7 2 ul EtOH GAPDH_VIC Unknown Undetermined H8 2 ul EtOH CCND1 Unknown Undetermined H8 2 ul EtOH GAPDH_VIC Unknown Undetermined H9 2 ul EtOH CCND1 Unknown Undetermined H9 2 ul EtOH GAPDH_VIC Unknown Undetermined H10 2 ul EtOH FOS Unknown Undetermined H10 2 ul EtOH GAPDH_VIC Unknown Undetermined H11 2 ul EtOH FOS Unknown Undetermined H11 2 ul EtOH GAPDH_VIC Unknown Undetermined H12 2 ul EtOH FOS Unknown Undetermined H12 2 ul EtOH GAPDH_VIC Unknown Undetermined ##### Sheet/List 2 ##### Document Name: Skupina 2 Plate Type: Standard Curve User: Student Document Information Operator: Administrator Run Date: Tuesday May 16 2017 14:17:40 Last Modified: Tuesday May 16 2017 16:13:47 Instrument Type: Applied Biosystems 7500 Real-Time PCR System Comments: SDS v1.4 Thermal Cycler Profile Stage Repetitions Temperature Time Ramp Rate 1 1 50.0 °C 2:00 100 2 1 95.0 °C 10:00 100 3 40 95.0 °C 0:15 100 60.0 °C 1:00 100 Standard 7500 Mode Data Collection : Stage 3 Step 2 PCR Volume: 25 µL Standard Curve Information Detector Name Slope Intercept R2 Number of Standards Number of Unknowns GAPDH_VIC -2.689338 34.924797 0.920620 36 21 CCND3 -2.468792 40.327991 0.841345 17 18 Jun -3.614629 50.110546 0.922626 8 14 Well Sample Name Detector Task Ct Qty A1 CCND3 GAPDH_VIC Standard 18.3935 150 A1 CCND3 CCND3 Standard 25.0702 150 A2 CCND3 GAPDH_VIC Standard 25.8427 15 A2 CCND3 CCND3 Standard 32.0229 15 A3 CCND3 GAPDH_VIC Standard 28.2354 1.5 A3 CCND3 CCND3 Standard 34.6097 1.5 A4 CCND3 GAPDH_VIC Standard 30.2413 0.15 A4 CCND3 CCND3 Standard 36.5557 0.15 A5 CCND3 GAPDH_VIC Standard 31.8654 0.015 A5 CCND3 CCND3 Standard 39.795 0.015 A6 CCND3 GAPDH_VIC Standard 33.6954 0.0015 A6 CCND3 CCND3 Standard 38.1574 0.0015 A7 JUN GAPDH_VIC Standard 19.2098 150 A7 JUN Jun Standard 30.941 150 A8 JUN GAPDH_VIC Standard 25.4387 15 A8 JUN Jun Standard 36.8211 15 A9 JUN GAPDH_VIC Standard 28.0151 1.5 A9 JUN Jun Standard 38.461 1.5 A10 JUN GAPDH_VIC Standard 30.0119 0.15 A10 JUN Jun Standard Undetermined 0.15 A11 JUN GAPDH_VIC Standard 31.8899 0.015 A11 JUN Jun Standard Undetermined 0.015 A12 JUN GAPDH_VIC Standard 33.9661 0.0015 A12 JUN Jun Standard Undetermined 0.0015 B1 CCND3 GAPDH_VIC Standard 18.5239 150 B1 CCND3 CCND3 Standard 25.0995 150 B2 CCND3 GAPDH_VIC Standard 25.8326 15 B2 CCND3 CCND3 Standard 32.0437 15 B3 CCND3 GAPDH_VIC Standard 28.2114 1.5 B3 CCND3 CCND3 Standard 34.4897 1.5 B4 CCND3 GAPDH_VIC Standard 30.3148 0.15 B4 CCND3 CCND3 Standard 36.0501 0.15 B5 CCND3 GAPDH_VIC Standard 32.0089 0.015 B5 CCND3 CCND3 Standard 38.504 0.015 B6 CCND3 GAPDH_VIC Standard 33.9079 0.0015 B6 CCND3 CCND3 Standard 37.7831 0.0015 B7 JUN GAPDH_VIC Standard 20.1451 150 B7 JUN Jun Standard 31.9663 150 B8 JUN GAPDH_VIC Standard 25.5433 15 B8 JUN Jun Standard 36.7994 15 B9 JUN GAPDH_VIC Standard 28.0885 1.5 B9 JUN Jun Standard 38.6304 1.5 B10 JUN GAPDH_VIC Standard 30.1984 0.15 B10 JUN Jun Standard Undetermined 0.15 B11 JUN GAPDH_VIC Standard 31.8479 0.015 B11 JUN Jun Standard Undetermined 0.015 B12 JUN GAPDH_VIC Standard 33.9928 0.0015 B12 JUN Jun Standard Undetermined 0.0015 C1 CCND3 GAPDH_VIC Standard 19.2388 150 C1 CCND3 CCND3 Standard 25.6467 150 C2 CCND3 GAPDH_VIC Standard 25.9362 15 C2 CCND3 CCND3 Standard 32.0763 15 C3 CCND3 GAPDH_VIC Standard 28.2938 1.5 C3 CCND3 CCND3 Standard 34.2669 1.5 C4 CCND3 GAPDH_VIC Standard 30.2787 0.15 C4 CCND3 CCND3 Standard 36.1434 0.15 C5 CCND3 GAPDH_VIC Standard 31.9479 0.015 C5 CCND3 CCND3 Standard Undetermined 0.015 C6 CCND3 GAPDH_VIC Standard 33.9829 0.0015 C6 CCND3 CCND3 Standard 38.6347 0.0015 C7 JUN GAPDH_VIC Standard 20.1399 150 C7 JUN Jun Standard 31.7631 150 C8 JUN GAPDH_VIC Standard 25.5344 15 C8 JUN Jun Standard 36.2194 15 C9 JUN GAPDH_VIC Standard 28.1155 1.5 C9 JUN Jun Standard Undetermined 1.5 C10 JUN GAPDH_VIC Standard 30.2569 0.15 C10 JUN Jun Standard Undetermined 0.15 C11 JUN GAPDH_VIC Standard 31.9398 0.015 C11 JUN Jun Standard Undetermined 0.015 C12 JUN GAPDH_VIC Standard 34.1667 0.0015 C12 JUN Jun Standard Undetermined 0.0015 D1 20% FBS CCND3 Unknown 29.1614 D2 20% FBS CCND3 Unknown 29.0937 D3 20% FBS CCND3 Unknown 29.1116 D4 bez FBS CCND3 Unknown 28.9889 D5 bez FBS CCND3 Unknown 28.9082 D6 bez FBS CCND3 Unknown 28.879 D9 noRT GAPDH_VIC Unknown 38.2706 D9 noRT CCND3 Unknown 39.4046 D10 noRT GAPDH_VIC Unknown Undetermined D10 noRT Jun Unknown 35.4734 D11 NK GAPDH_VIC Unknown 38.5132 D11 NK CCND3 Unknown 38.9134 D12 NK GAPDH_VIC Unknown Undetermined D12 NK Jun Unknown Undetermined E1 20% FBS Jun Unknown 33.7131 E2 20% FBS Jun Unknown 33.7083 E3 20% FBS Jun Unknown 33.3043 E4 bez FBS Jun Unknown 32.7198 E5 bez FBS Jun Unknown 32.4231 E6 bez FBS Jun Unknown 32.241 E9 noRT GAPDH_VIC Unknown 37.7673 E9 noRT CCND3 Unknown 37.439 E10 noRT GAPDH_VIC Unknown Undetermined E10 noRT Jun Unknown 35.8698 E11 NK GAPDH_VIC Unknown 38.4244 E11 NK CCND3 Unknown Undetermined E12 NK GAPDH_VIC Unknown Undetermined E12 NK Jun Unknown Undetermined F1 20% FBS GAPDH Unknown 20.2913 F2 20% FBS GAPDH Unknown 20.7614 F3 20% FBS GAPDH Unknown 20.3764 F4 bez FBS GAPDH Unknown 20.0838 F5 bez FBS GAPDH Unknown 20.0465 F6 bez FBS GAPDH Unknown 20.0074 G1 20% FBS GAPDH_VIC Unknown 22.4943 G1 20% FBS CCND3 Unknown 28.6324 G2 20% FBS GAPDH_VIC Unknown 22.507 G2 20% FBS CCND3 Unknown 28.627 G3 20% FBS GAPDH_VIC Unknown 22.4893 G3 20% FBS CCND3 Unknown 28.732 G4 bez FBS GAPDH_VIC Unknown 22.2209 G4 bez FBS CCND3 Unknown 28.5485 G5 bez FBS GAPDH_VIC Unknown 22.1242 G5 bez FBS CCND3 Unknown 28.4723 G6 bez FBS GAPDH_VIC Unknown 22.1082 G6 bez FBS CCND3 Unknown 28.4504 G7 20% FBS 1 ul EtOH GAPDH_VIC Unknown 27.3844 G7 20% FBS 1 ul EtOH CCND3 Unknown 30.1352 G8 20% FBS 1 ul EtOH GAPDH_VIC Unknown 27.6088 G8 20% FBS 1 ul EtOH CCND3 Unknown 30.2859 G9 20% FBS 1 ul EtOH GAPDH_VIC Unknown 29.7969 G9 20% FBS 1 ul EtOH CCND3 Unknown 30.8013 G10 bez FBS 1 ul EtOH GAPDH_VIC Unknown 27.9581 G10 bez FBS 1 ul EtOH CCND3 Unknown Undetermined G11 bez FBS 1 ul EtOH GAPDH_VIC Unknown Undetermined G11 bez FBS 1 ul EtOH CCND3 Unknown Undetermined G12 bez FBS 1 ul EtOH GAPDH_VIC Unknown 35.3945 G12 bez FBS 1 ul EtOH CCND3 Unknown Undetermined H1 20% FBS GAPDH_VIC Unknown 22.0656 H1 20% FBS Jun Unknown 33.4399 H2 20% FBS GAPDH_VIC Unknown 22.3191 H2 20% FBS Jun Unknown 33.5406 H3 20% FBS GAPDH_VIC Unknown 22.1659 H3 20% FBS Jun Unknown 33.5191 H4 bez FBS GAPDH_VIC Unknown 21.943 H4 bez FBS Jun Unknown 32.6125 H5 bez FBS GAPDH_VIC Unknown 21.907 H5 bez FBS Jun Unknown 32.5121 H6 bez FBS GAPDH_VIC Unknown 21.9092 H6 bez FBS Jun Unknown 32.2909 H7 20% FBS 2 ul EtOH GAPDH_VIC Unknown Undetermined H7 20% FBS 2 ul EtOH Jun Unknown Undetermined H8 20% FBS 2 ul EtOH GAPDH_VIC Unknown Undetermined H8 20% FBS 2 ul EtOH Jun Unknown Undetermined H9 20% FBS 2 ul EtOH GAPDH_VIC Unknown Undetermined H9 20% FBS 2 ul EtOH Jun Unknown Undetermined H10 bez FBS 2 ul EtOH GAPDH_VIC Unknown Undetermined H10 bez FBS 2 ul EtOH Jun Unknown Undetermined H11 bez FBS 2 ul EtOH GAPDH_VIC Unknown Undetermined H11 bez FBS 2 ul EtOH Jun Unknown Undetermined H12 bez FBS 2 ul EtOH GAPDH_VIC Unknown Undetermined H12 bez FBS 2 ul EtOH Jun Unknown Undetermined