MENDEL UNIVERSITY IN BRNO
martincerny83@gmail.com
Department of Molecular Biology and Radiobiology
umbr.af.mendelu.cz
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MENDEL UNIVERSITY IN BRNO
Martin Černý
Developmental and Production Biology – Omics Approaches
MENDEL UNIVERSITY IN BRNO
225 Department of Molecular Biology and Radiobiology
Developmental and Production Biology – Omics Approaches
Who are we?
6 postdocs
6 PhD students
6 graduate students
14+ pregraduate students
2 technicians
• Plant molecular physiology&Radiobiology
• Proteomics&Metabolomics
• Structural biologyhttp://umbr.af.mendelu.cz
Prof. RNDr. Břetislav Brzobohatý, CSc.
MENDEL UNIVERSITY IN BRNO
root growth
nutrient
uptake
leaf
development
pathogen
interaction
light
temperature
yield
aging
clock
flowering
Omics group
MENDEL UNIVERSITY IN BRNO
microscopy proteomics metabolomics
transformation
RT-qPCR
bioinformatics
MENDEL UNIVERSITY IN BRNO
Omics analyses
MENDEL UNIVERSITY IN BRNO
• Proteome profiling
• Relative and absolute
protein quantification
• Metabolome profiling
maXis impact
UHR-TOF
Protein-ligand
interaction
LESA
Nanomate
Detection of PTMs
amaZon speed ETD
ion trap
TSQ Quantiva
triple quadrupole MS
Targeted analyses
ag sensitivity
Our LC-MS
MENDEL UNIVERSITY IN BRNOQ Exactive GC Orbitrap
5/2017
MENDEL UNIVERSITY IN BRNO
Abiotic interactions
- Temperature perception
- Light quality, inensity, photoperiod
Biotic interactions
- Phytophthora
- Club root disease
Molecular processes and grwoth regulation
- UPS
- Phytohormones, growth regulators
Topics
MENDEL UNIVERSITY IN BRNO
“Cytokinin modulates proteomic,
transcriptomic and growth responses
to temperature shocks in Arabidopsis“
“The impact of heat stress targeting
on the hormonal and transcriptomic
response in Arabidopsis“
Abiotic interactions:
Cytokinin and temperature
MENDEL UNIVERSITY IN BRNOAbiotic interactions:
Cytokinin and temperature
MENDEL UNIVERSITY IN BRNOAbiotic interactions:
Cytokinin and temperature
2017-2019 GACR 17-04607S – Interaction between light and cytokinin
2017-2019 – PhD Talent – Role of HSP proteins in regulation of seed germination
Institute of
Experimental
Botany AS CR
Ing. Hana Habánová
RNDr. Radomíra Vaňková, CSc.
MENDEL UNIVERSITY IN BRNO
Phytophthora
2016-2022
MENDEL UNIVERSITY IN BRNOPlant signaling:
Biotic and abiotic interactions
New joint research centre at Mendel University
Our role:
Comparative analysis of Phytophthora infection
Plant hormones in Phytophthora infection
Abiotic stress and Phytophthora infection
MENDEL UNIVERSITY IN BRNO
Club root disease
MENDEL UNIVERSITY IN BRNOUPS in plant signaling
Andreas Bachmair
2017-2018 7AMB17AT027:
„Targeted protein degradation in
response to a plant hormone
cytokinine“).
MENDEL UNIVERSITY IN BRNO
Plant-plant interactions
CEITEC 2020
TE02000177 - Centrum pro inovativní využití a posílení
konkurenceschopnosti českých pivovarských surovin a
výrobků (2014-2019, TA0/TE)
- Stress response propagation
- Volatile compounds
MENDEL UNIVERSITY IN BRNO
Peptide libraries for targeted protein
identification and quantitation
Techniques development and
optimization
Targeted Metabolomics