Physical-chemistry methods
for study of protein-protein
interactions involved in
neurodegenerative diseases
JOZEF HRITZ
jozef.hritz@ceitec.muni.cz
CEITEC MU, Structural Biology, building A4, office:2.11
Biophysical Chemistry
RESEARCH OBJECT: 14-3-3/complexes
>500
partners
NMR: REGULATORY DOMAIN
14-3-3: BINDING MODES
Hritz J.; Byeon I-J.; Krzysiak T.; Martinez A.; Sklenář V.; Gronenborn A.M. Dissection of binding between a
phosphorylated tyrosine hydroxylase peptide and 14-3-3ζ: a complex story elucidated by NMR. Biophys. J. 2014, 107,
2185-2194
14-3-3: NEURODEGENERATION
Alzheimer
Parkinson
Lateral sclerosis
1%
ALZHEIMER DISEASE: 14-3-3+Aβ
B
IH
FE
CB
IH
FE
CB
IH
FE
C
BIOPHYSICAL
CHEMISTRY
14-3-3 KINETIC ASSAY: FRET
Experimental Biophysical Chemistry• – fluorescence assays
Computational Biophysical Chemistry• – structural and free energy calculations
of dimer/monomer equilibria
HANDS ON EXPERIENCE
CONFERENCES AND INTERNSHIPS
SUITABLE CANDIDATES
OPEN POSITIONS
jozef.hritz@ceitec.muni.cz