Phenoloxidase ESP 4/2/2019 15:20 Assay was completed successfully. Results Well Sample Cycles Maximum slope (1/cycle) A1 A1 7 0.005 A2 A2 7 0.002 A3 A3 7 0.002 A4 A4 7 0.003 A5 A5 7 0.004 B1 B1 7 0.435 B2 B2 7 0.08 B3 B3 7 0.014 B4 B4 7 0.315 B5 B5 7 0.155 C1 C1 7 0.293 C2 C2 7 0.089 C3 C3 7 0.026 C4 C4 7 0.352 C5 C5 7 0.178 D1 D1 7 0.011 D2 D2 7 0.007 D3 D3 7 0.009 D4 D4 7 0.047 D5 D5 7 0.069 E1 E1 7 0.008 F1 F1 7 0.18 G1 G1 7 0.439 H1 H1 7 0.045 ##### Sheet/List 2 ##### Phenoloxidase ESP 4/2/2019 15:20 Assay was completed successfully. Replicate statistics Absorbance Maximum slope (1/cycle) Sample Dilution N CV Mean A1 All 1 0.00% 0.005 A1 1:1 1 0.00% 0.005 A2 All 1 0.00% 0.002 A2 1:1 1 0.00% 0.002 A3 All 1 0.00% 0.002 A3 1:1 1 0.00% 0.002 A4 All 1 0.00% 0.003 A4 1:1 1 0.00% 0.003 A5 All 1 0.00% 0.004 A5 1:1 1 0.00% 0.004 B1 All 1 0.00% 0.435 B1 1:1 1 0.00% 0.435 B2 All 1 0.00% 0.08 B2 1:1 1 0.00% 0.08 B3 All 1 0.00% 0.014 B3 1:1 1 0.00% 0.014 B4 All 1 0.00% 0.315 B4 1:1 1 0.00% 0.315 B5 All 1 0.00% 0.155 B5 1:1 1 0.00% 0.155 C1 All 1 0.00% 0.293 C1 1:1 1 0.00% 0.293 C2 All 1 0.00% 0.089 C2 1:1 1 0.00% 0.089 C3 All 1 0.00% 0.026 C3 1:1 1 0.00% 0.026 C4 All 1 0.00% 0.352 C4 1:1 1 0.00% 0.352 C5 All 1 0.00% 0.178 C5 1:1 1 0.00% 0.178 D1 All 1 0.00% 0.011 D1 1:1 1 0.00% 0.011 D2 All 1 0.00% 0.007 D2 1:1 1 0.00% 0.007 D3 All 1 0.00% 0.009 D3 1:1 1 0.00% 0.009 D4 All 1 0.00% 0.047 D4 1:1 1 0.00% 0.047 D5 All 1 0.00% 0.069 D5 1:1 1 0.00% 0.069 E1 All 1 0.00% 0.008 E1 1:1 1 0.00% 0.008 F1 All 1 0.00% 0.18 F1 1:1 1 0.00% 0.18 G1 All 1 0.00% 0.439 G1 1:1 1 0.00% 0.439 H1 All 1 0.00% 0.045 H1 1:1 1 0.00% 0.045 ##### Sheet/List 3 ##### Phenoloxidase ESP 4/2/2019 15:20 Assay was completed successfully. Raw OD(492) Plate # Well Cycle # 1 2 3 4 5 6 7 1 A1 Time (s) 0 296.643 592.705 888.786 1184.911 1480.982 1777.08 1 A1 Result 0.157 0.164 0.168 0.17 0.172 0.176 0.178 1 A2 Time (s) 0.643 297.277 593.348 889.429 1185.554 1481.625 1777.723 1 A2 Result 0.095 0.097 0.099 0.1 0.102 0.102 0.1 1 A3 Time (s) 1.286 297.92 593.991 890.072 1186.197 1482.268 1778.366 1 A3 Result 0.923 0.918 0.914 0.91 0.889 0.894 0.893 1 A4 Time (s) 1.911 298.545 594.616 890.697 1186.822 1482.893 1778.991 1 A4 Result 0.152 0.156 0.159 0.158 0.158 0.157 0.156 1 A5 Time (s) 2.545 299.179 595.25 891.33 1187.455 1483.527 1779.625 1 A5 Result 0.939 0.942 0.94 0.943 0.948 0.944 0.94 1 B1 Time (s) 5.67 302.277 598.339 894.402 1190.5 1486.572 1782.652 1 B1 Result 0.38 0.876 1.25 1.478 1.622 1.73 1.815 1 B2 Time (s) 5.027 301.634 597.697 893.759 1189.857 1485.938 1782.009 1 B2 Result 0.082 0.109 0.14 0.169 0.205 0.262 0.364 1 B3 Time (s) 4.402 301.018 597.072 893.134 1189.232 1485.313 1781.393 1 B3 Result 0.944 0.948 0.953 0.966 0.978 0.99 1.007 1 B4 Time (s) 3.759 300.384 596.447 892.527 1188.643 1484.732 1780.813 1 B4 Result 0.263 0.471 0.806 1.1 1.255 1.364 1.48 1 B5 Time (s) 3.17 299.804 595.875 891.955 1188.08 1484.161 1780.25 1 B5 Result 1.276 1.484 1.586 1.663 1.758 1.864 1.971 1 C1 Time (s) 6.304 302.902 598.938 894.982 1191.063 1487.143 1783.214 1 C1 Result 1.187 1.574 1.773 1.851 1.981 2.061 2.159 1 C2 Time (s) 6.911 303.473 599.5 895.545 1191.634 1487.705 1783.777 1 C2 Result 0.394 0.474 0.536 0.613 0.702 0.791 0.87 1 C3 Time (s) 7.545 304.107 600.125 896.17 1192.259 1488.33 1784.393 1 C3 Result 1.037 1.072 1.087 1.114 1.127 1.151 1.179 1 C4 Time (s) 8.161 304.723 600.741 896.786 1192.866 1488.938 1785 1 C4 Result 0.775 1.246 1.478 1.64 1.761 1.859 1.964 1 C5 Time (s) 8.786 305.313 601.322 897.348 1193.429 1489.5 1785.563 1 C5 Result 1.374 1.592 1.729 1.823 1.92 2.015 2.103 1 D1 Time (s) 11.938 308.447 604.447 900.473 1196.554 1492.616 1788.679 1 D1 Result 0.128 0.132 0.145 0.154 0.157 0.163 0.171 1 D2 Time (s) 11.295 307.804 603.804 899.83 1195.911 1491.973 1788.036 1 D2 Result 0.077 0.086 0.092 0.098 0.103 0.107 0.112 1 D3 Time (s) 10.652 307.161 603.161 899.188 1195.268 1491.33 1787.393 1 D3 Result 0.168 0.178 0.183 0.19 0.2 0.2 0.208 1 D4 Time (s) 10.009 306.518 602.518 898.554 1194.634 1490.697 1786.759 1 D4 Result 0.194 0.239 0.287 0.329 0.367 0.406 0.447 1 D5 Time (s) 9.366 305.884 601.884 897.92 1194 1490.063 1786.125 1 D5 Result 0.289 0.364 0.427 0.467 0.504 0.537 0.57 1 E1 Time (s) 12.572 309.089 605.089 901.116 1197.197 1493.268 1789.322 1 E1 Result 0.15 0.163 0.166 0.169 0.17 0.171 0.174 1 F1 Time (s) 13.214 309.723 605.732 901.75 1197.839 1493.902 1789.964 1 F1 Result 0.197 0.327 0.441 0.556 0.713 0.909 1.073 1 G1 Time (s) 13.866 310.366 606.375 902.384 1198.464 1494.527 1790.58 1 G1 Result 0.807 1.434 1.685 1.902 2.017 2.111 2.194 1 H1 Time (s) 14.482 310.938 606.947 902.947 1199.027 1495.089 1791.134 1 H1 Result 0.203 0.251 0.294 0.342 0.38 0.413 0.44 ##### Sheet/List 4 ##### Phenoloxidase ESP 4/2/2019 15:20 Assay was completed successfully. Info Instrument Instrument serial number 3180172 Instrument firmware version 103 Software Version 0.5.54.0 Plate Wells 96 (8x12) Manufacturer General Name A1 offset (µm) "14380,11240" Well distance (µm) 9000 Well diameter (µm) 6500 Operations Kinetics by plate Cycles 7 Interval 0:05:00 Shake No shake Absorbance Technology Absorbance Flashes 25 Calculation OD(492) End kinetics