Phenoloxidase ESP 4/2/2019 17:08 Assay was completed successfully. Results Well Sample Cycles Maximum slope (1/cycle) A6 A6 7 0.006 A7 A7 7 0.001 A8 A8 7 0.019 A9 A9 7 0.016 B6 B6 7 0.363 B7 B7 7 0.005 B8 B8 7 0.331 B9 B9 7 0.244 C6 C6 7 0.263 C7 C7 7 0.025 C8 C8 7 0.309 C9 C9 7 0.167 D6 D6 7 0.046 D7 D7 7 0.015 D8 D8 7 0.044 D9 D9 7 0.044 ##### Sheet/List 2 ##### Phenoloxidase ESP 4/2/2019 17:08 Assay was completed successfully. Replicate statistics Absorbance Maximum slope (1/cycle) Sample Dilution N CV Mean A6 All 1 0.00% 0.006 A6 1:1 1 0.00% 0.006 A7 All 1 0.00% 0.001 A7 1:1 1 0.00% 0.001 A8 All 1 0.00% 0.019 A8 1:1 1 0.00% 0.019 A9 All 1 0.00% 0.016 A9 1:1 1 0.00% 0.016 B6 All 1 0.00% 0.363 B6 1:1 1 0.00% 0.363 B7 All 1 0.00% 0.005 B7 1:1 1 0.00% 0.005 B8 All 1 0.00% 0.331 B8 1:1 1 0.00% 0.331 B9 All 1 0.00% 0.244 B9 1:1 1 0.00% 0.244 C6 All 1 0.00% 0.263 C6 1:1 1 0.00% 0.263 C7 All 1 0.00% 0.025 C7 1:1 1 0.00% 0.025 C8 All 1 0.00% 0.309 C8 1:1 1 0.00% 0.309 C9 All 1 0.00% 0.167 C9 1:1 1 0.00% 0.167 D6 All 1 0.00% 0.046 D6 1:1 1 0.00% 0.046 D7 All 1 0.00% 0.015 D7 1:1 1 0.00% 0.015 D8 All 1 0.00% 0.044 D8 1:1 1 0.00% 0.044 D9 All 1 0.00% 0.044 D9 1:1 1 0.00% 0.044 ##### Sheet/List 3 ##### Phenoloxidase ESP 4/2/2019 17:08 Assay was completed successfully. Raw OD(492) Plate # Well Cycle # 1 2 3 4 5 6 7 1 A6 Time (s) 0 296.857 593.152 889.42 1185.715 1482.054 1778.313 1 A6 Result 0.335 0.343 0.347 0.346 0.349 0.352 0.353 1 A7 Time (s) 0.643 297.491 593.786 890.054 1186.348 1482.688 1778.947 1 A7 Result 0.077 0.078 0.079 0.078 0.079 0.079 0.08 1 A8 Time (s) 1.286 298.134 594.429 890.697 1186.991 1483.331 1779.59 1 A8 Result 0.398 0.425 0.437 0.445 0.452 0.462 0.472 1 A9 Time (s) 1.92 298.768 595.063 891.331 1187.616 1483.965 1780.223 1 A9 Result 0.428 0.455 0.46 0.468 0.48 0.488 0.494 1 B6 Time (s) 4.393 301.179 597.465 893.741 1190.018 1486.348 1782.59 1 B6 Result 0.864 1.385 1.59 1.712 1.79 1.858 1.919 1 B7 Time (s) 3.75 300.536 596.822 893.098 1189.375 1485.706 1781.947 1 B7 Result 0.076 0.08 0.087 0.091 0.097 0.101 0.105 1 B8 Time (s) 3.143 299.965 596.259 892.527 1188.813 1485.143 1781.384 1 B8 Result 1.141 1.686 1.803 1.953 2.018 2.107 2.25 1 B9 Time (s) 2.554 299.393 595.688 891.965 1188.25 1484.59 1780.848 1 B9 Result 1.466 1.775 1.954 2.09 2.211 2.359 2.502 1 C6 Time (s) 5.018 301.759 598.036 894.304 1190.581 1486.911 1783.152 1 C6 Result 1.273 1.62 1.799 1.902 2.017 2.103 2.167 1 C7 Time (s) 5.607 302.331 598.607 894.866 1191.143 1487.473 1783.706 1 C7 Result 0.211 0.225 0.251 0.275 0.301 0.323 0.347 1 C8 Time (s) 6.25 302.973 599.241 895.509 1191.786 1488.107 1784.348 1 C8 Result 1.432 1.912 2.05 2.137 2.234 2.347 2.489 1 C9 Time (s) 6.822 303.536 599.804 896.072 1192.34 1488.661 1784.884 1 C9 Result 1.574 1.764 1.858 1.992 2.073 2.18 2.406 1 D6 Time (s) 9.313 306.018 602.286 898.545 1194.813 1491.125 1787.331 1 D6 Result 0.356 0.397 0.448 0.482 0.517 0.554 0.594 1 D7 Time (s) 8.67 305.375 601.643 897.902 1194.17 1490.482 1786.688 1 D7 Result 0.101 0.117 0.132 0.145 0.157 0.171 0.183 1 D8 Time (s) 8.036 304.741 601.009 897.268 1193.536 1489.848 1786.063 1 D8 Result 0.352 0.402 0.439 0.476 0.509 0.54 0.567 1 D9 Time (s) 7.402 304.107 600.375 896.634 1192.902 1489.223 1785.429 1 D9 Result 0.341 0.385 0.43 0.466 0.503 0.537 0.568 ##### Sheet/List 4 ##### Phenoloxidase ESP 4/2/2019 17:08 Assay was completed successfully. Info Instrument Instrument serial number 3180172 Instrument firmware version 103 Software Version 0.5.54.0 Plate Wells 96 (8x12) Manufacturer General Name A1 offset (µm) "14380,11240" Well distance (µm) 9000 Well diameter (µm) 6500 Operations Kinetics by plate Cycles 7 Interval 0:05:00 Shake No shake Absorbance Technology Absorbance Flashes 25 Calculation OD(492) End kinetics