File:
Print Date: User:
Plate Type: PCR Volume:
Doci
Document Information
Operator: Run Date: Last Modified: Instrument Type:
7.2.19_PD test
Monday, February 11, 2019 11:02:06 Administrator Standard Curve 40 uL
Administrator
Thursday, February 07, 2019 15:04:08 Thursday, February 07, 2019 18:13:27 Applied Biosystems 7300 Real-Time PCR System
Comments
SDS vl.4
					
Thermal Profile					
					
Stage	Temperature	Time	Repeat	Ramp Rate	Auto Increment
1	37.0 °C	2:00	1	Auto	
2	95.0 °C	15:00	1	Auto	
3	95.0 °C	0:05	45	Auto	
	60.0 °C	0:40		Auto	
	72.0 °C	0:20		Auto	
Standard 7300 Mode
Data Collection : Stage 3, Step 2
Analysis Methods:
Delta Rn
Detector Information
Detector Name
Reporter
Quencher
Threshold
Baseline Start
Baseline End
7.2.19 PDtest
Page 1 of 4 (02/11/19 11:02:06)
SYBR Green
SYBR
(none)
<0.747206>
Auto
Auto
L
Well  Sample Name Detector
Al A2 Bl B2
Task
StdDev Ct   Quantity     Mean Qty   StdDev Qty Filtered Tm
SYBR Green	NTC	Undet.
SYBR Green	Unknown	31.0733
SYBR Green	Standard	34
SYBR Green	Standard	29.6971
SYBR Green	Standard	26.2582
SYBR Green	Standard	23.0192
SYBR Green	Standard	19.4098
User Defined User Defined User Defined #1 #2 #3
7.2.19_PD test
Page 2 of 4 (02/11/19 11:02:06)
Raw Data
1.504e+006
1.400e+006
1.200e+006
1.000e+006
CD Ü
CD Ü (/) CD
O 3
8.000e+005
6.000e+005
4.000e+005
2.000e+005
0.000e+000
Filters
Reading: 1
Well(s): A1-A5.B1-B5
7.2.19_PD test
Page 3 of 4 (02/11/19 11:02:06)
Delta Rn vs Cycle
1.000e+004
1.000e+003
1.000e+002
1.000e+001
1.000e+000
DC
H—I
CD Q
1.000e-001
1.000e-002
1.000e-003
1.000e-004
1.000e-005
1    3   5    7   9   11   13 15 17 19 21  23 25 27 29 31  33 35 37 39 41  43 45
Selected Detector: All Well(s): A1-A5,B1-B5
Cycle Number
7.2.19_PD test
Page 4 of 4 (02/11/19 11:02:06)