File:
Print Date: User:
Plate Type: PCR Volume:
Doci
Document Information
Operator: Run Date: Last Modified: Instrument Type:
SMAM 15.4.
Tuesday, April 16, 2019 16:22:22 Administrator Standard Curve 40 uL
Administrator
Monday, April 15, 2019 17:10:58 Monday, April 15, 2019 18:50:50 Applied Biosystems 7300 Real-Time PCR System
Comments
SDS vl.4
					
Thermal Profile					
					
Stage	Temperature	Time	Repeat	Ramp Rate	Auto Increment
1	37.0 °C	2:00	1	Auto	
2	95.0 °C	15:00	1	Auto	
3	95.0 °C	0:05	45	Auto	
	60.0 °C	0:40		Auto	
	72.0 °C	0:27		Auto	
Standard 7300 Mode
Data Collection : Stage 3, Step 3
Analysis Methods:
Delta Rn
Detector Information
Detector Name
Reporter
Quencher
Threshold
Baseline Start
Baseline End
SMAM 15.4.
Page 1 of 4 (04/16/19 16:22:22)
SYBR Green
SYBR
(none)
<0.134109>
Auto
Auto
Standard Curve Information
]
□
Detector Name
Slope
Intercept
R2
Standards
Unknowns
SYBR Green
-4.226952
35.989067
0.988742
Well   Sample Name Detector
Task
Ct
StdDev Ct   Quantity     Mean Qty   StdDev Qty Filtered Tm
User Defined User Defined User Defined #1 #2 #3
SYBR	Green	Unknown	Undet.
SYBR	Green	Unknown	Undet.
SYBR	Green	Unknown	44.0298
SYBR	Green	Unknown	Undet.
SYBR	Green	Unknown	Undet.
SYBR	Green	Unknown	28.0272
SYBR	Green	NTC	Undet.
SYBR	Green	Standard	32.2665
SYBR	Green	Standard	26.6947
SYBR	Green	Standard	23.4759
SYBR	Green	Standard	19.2496
SYBR	Green	Standard	Undet.
0.0125243
100
1.0e+003 1.0e+004
SMAM 15.4.
Page 2 of 4 (04/16/19 16:22:22)
Raw Data
CD Ü
CD Ü </) CD
O 3
1.240e+006
1.150e+006
9.500e+005
7.500e+005
5.500e+005
3.500e+005
1.500e+005
4.220e+004
Reading: 1
Well(s): A1-A7,B1-B5
Filters
SMAM 15.4.
Page 3 of 4 (04/16/19 16:22:22)
Delta Rn vs Cycle
1.000e+004
1.000e+003
1.000e+002
1.000e+001
DC
H—I
CD Q
1.000e+000
1.000e-001
1.000e-002
1.000e-003
1.000e-004
13 15 17 19 21  23 25 27 29 31  33 35 37 39 41  43 45
Cycle Number
Selected Detector: All Well(s): A1-A7.B1-B5
SMAM 15.4.
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