AMP 3/4/2019 12:23 Assay was completed successfully. Results Well Sample Cycles A1 A1 3 B1 B1 3 C1 C1 3 D1 D1 3 ##### Sheet/List 2 ##### AMP 3/4/2019 12:23 Assay was completed successfully. Replicate statistics Luminescence Cycles Sample Dilution N CV Mean A1 All 1 0.00% 3 A1 1:1 1 0.00% 3 B1 All 1 0.00% 3 B1 1:1 1 0.00% 3 C1 All 1 0.00% 3 C1 1:1 1 0.00% 3 D1 All 1 0.00% 3 D1 1:1 1 0.00% 3 ##### Sheet/List 3 ##### AMP 3/4/2019 12:23 Assay was completed successfully. Luminescence (counts) Plate # Well Cycle # 1 2 3 1 A1 Time (s) 0 4.991 9.973 1 A1 Result 984 1159 958 1 B1 Time (s) 1.143 6.143 11.125 1 B1 Result 260567 260025 260039 1 C1 Time (s) 2.285 7.285 12.277 1 C1 Result 624 780 653 1 D1 Time (s) 3.428 8.428 13.419 1 D1 Result 275 289 248 ##### Sheet/List 4 ##### AMP 3/4/2019 12:23 Assay was completed successfully. Info Instrument Instrument serial number 3180172 Instrument firmware version 103 Software Version 0.5.54.0 Plate Wells 96 (8x12) Manufacturer General Name A1 offset (µm) "14380,11240" Well distance (µm) 9000 Well diameter (µm) 6500 Operations Kinetics by plate Cycles 3 Interval 0:00:00 Shake No shake Luminescence Technology Luminescence Filter IR Cutoff Counting time (s) 1 Aperture Default Read mode Top Focus (mm) Default PMT voltage Default Discriminator voltage Default End kinetics