C8953
NMR strukturní analýza
semináˇr
COSY & NOESY
Jan Novotný, Kateˇrina Peterková
176003@is.muni.cz
March 27, 2019
COSY : Atropine
COSY : Atropine
8
8
6
6
4
4
2
2
8 8
6 6
4 4
2 2
δ1-1H(ppm)
COSY : Atropine5 4 3 2 1
5 5
4 4
3 3
2 2
δ1-1
H(ppm)
NOESY - introduction
Nuclear Overhauser effect
dipol-dipol interaction
magnetisation transfer TROUGH
SPACE as a consequence of
cross-relaxation
NOESY
correlates nuclei if their distance is
smaller than 5 Å
acq
t1 t2
90 9090
P E M D
tmix
NOE differential experiment
Colchicine - DQF-COSY
8
8
6
6
4
4
2
2
8 8
6 6
4 4
2 2
δ1-1
H(ppm)
54
6
3
1
2
11
10 16
14
15
7
8
9 NH
17
18CH3
19
O
O
O O
CH3
22
CH3
21
CH3
20
O
CH3 23
O
12
13
Colchicine - NOESY
8 8
6 6
4 4
2 2
δ1-1H(ppm)
54
6
3
1
2
11
10 16
14
15
7
8
9 NH
17
18CH3
19
O
O
O O
CH3
22
CH3
21
CH3
20
O
CH3 23
O
12
13
Next session:
Midterm Test, Heteronuclear correlations