SIMULTANEOUS IMAGING OF PROTEINS AND ELEMENTS IN TISSUES
BY LA-ICP-MS
Michaela TVRDOŇOVÁ 1,2
, Lena ASCHER3
, Norbert JAKUBOWSKI3
,
Marketa VACULOVIČOVÁ4,5
, Marcela VLČNOVSKÁ5
, Viktor KANICKÝ1
and Tomas
VACULOVIČ 1,2
1
Department of Chemistry, Faculty of Science, Masaryk University in Brno, Kotlarska 2, 611 37
Brno,
2
CEITEC Masaryk University in Brno Kamenice 5, 625 00 Brno,
3
BAM Federal Institut for Materials Research and Testing, Richard-Willstätter-Straße 11,
12489 Berlin
4
Brno Univ Technol, Cent European Inst Technol, Purkynova 123, CZ-61200 Brno, Czech
Republic,
5
Mendel Univ Brno, Dept Chem & Biochem, Zemedelska 1, CZ-61300 Brno, Czech Republic
Email of presenting author: 358018@mail.muni.cz
Laser ablation with inductively coupled plasma is still more used in life science as biology and
biomedicine and the utilization of metals and proteins determination simultaneously is also
growing up. 1,2
We have developed a new strategy of antibody labeling (it can specific binds to
proteins) by nanoparticles which is composed of thousands of atoms and thus increases the
sensitivity enormously and of course decreases the limit of detection, compare to lanthanoids
labeling. The ability of successfully tagged antibodies bounds to antigen (protein) was proved by
dot blot on membrane imaged by LA-ICP-MS. It should be universal method to image
simultaneously distribution of elements and proteins as well. One of the many possible
applications is binding of labelled antibodies to the tumour tissues containing metallothionein
(known as a transporter of Cu, Zn and heavy metals) and image their distribution in different
tumour stage.
References
1. L. Mueller, A. J. Herrmann, S. Techritz, U. Panne, and N. Jakubowski, Quantitative
characterization of single cells by use of immunocytochemistry combined with multiplex LAICP-MS.
Analytical and Bioanalytical Chemistry, 409, (2017):3667.
2. S. Kanje, A. J. Herrmann, S. Hober, and L. Mueller, Next generation of labeling reagents for
quantitative and multiplexing immunoassays by the use of LA-ICP-MS. Analyst, 141,
(2016):6374.
Acknowledgements
Czech Science Foundation (project No. 17-12774S). Ministry of Education, Youth and Sports of
the Czech Republic under the project CEITEC 2020 (LQ1601). MUNI/A/ 1288/2017