Poster: Epoxidation is Preferred Way of First-stage Metabolism of Abiraterone Acetate in Brown
Bullhead (Ameiurus Nebulosus)


Samuel Mach^*1,3, Alexandr Jegorov^1, Marek Kuzma^2, Jakub Zápal^2, Zdeněk Šimek^3, Jan Čejka^4,
Václav Eigner^4

^*corresponding author: samuel.mach@tevapharm.cz, tel: +420553642640

^1 Teva Czech Industries, s.r.o., Ostravská 29, 747 70 Opava Komárov, Czech Republic

^2 Academy of Sciences of the Czech Republic, Inst. of Microbiology, Videňská 1083, 142 20 Prague 4

^3 Masaryk University Brno, RECETOX, Kamenice 126/3, 625 00 Brno, Czech Republic

^4 Prague Institute of Chemical Technology, Technická 5, 166 28 Prague 6


Abstract: Expected both primary (products of hydroxylation) and secondary (products of
glucuronidation and sulphatation) metabolites of abiraterone acetate prodrug were identified in
aquarium water containing excreted metabolites from brown bullhead (Ameiurus nebulosus) by means of
HPLC/MS. NMR measurement of a prevailing metabolite presumed to be one of possible
hydroxy-abiraterones discovered that it is not hydroxy-abiraterone but abiraterone-16,17-epoxide.
Closer analysis of MS^2 and MS^3 spectra revealed that also some secondary metabolites are probably
16,17-epoxides.