SALES ORDER NO: 8818373237 CUSTOMER NO: 0000002011 SHIPMENT DATE: 15/03/2022 INSTITUTE: Prirodovedecká fakulta MU RESEARCHER: Prirodovedecká fakulta MU PURCHASE ORDER NO: 3112/0093/22 Bin Number: 803* Calculation is provided for oligos shipped dry. Batch # Oligo Name Oligo # Len Pur Scale MW Tm° µg/OD OD µg nmol Epsilon 1/(mMcm) Dimer2ndry GC % µl for 100µM* Sequence(5'-3') HA15562793 GOx_cHIS_FW 8818373237-20/0 34 DST 0.025 10379 80.7 32.6 10.44 341.0 32.8 317.7 No Moderate 55.8 328 ATATACCATGGGCCAGACCCTCCTTGTGAGCTCG HA15562794 GOx_cHIS_Rev 8818373237-30/0 32 DST 0.025 9968 73.6 30.5 15.4 470.9 47.2 326 No Weak 46.8 472 ATATACTCGAGGATGAGCAAGGCGGTAGTGTT HA15562795 GOx_NHIS_FW 8818373237-40/0 32 DST 0.025 9760 76.2 32.2 15.56 502.2 51.4 302.4 No Moderate 53.1 514 ATATAGGATCCCAGACCCTCCTTGTGAGCTCG HA15562796 GOx_NHIS_Rev 8818373237-50/0 32 DST 0.025 9937 71.6 30.3 14.88 451.9 45.4 327.2 No Weak 46.8 454 ATATACTCGAGCTAGATGAGCAAGGCGGTAGT Technical Datasheet Centrifuge tube prior to opening to prevent loss of pelleted oligonucleotide. For Research Use Only Key to Symbols: R = A + G, Y = C + T, M = A + C, K = G + T, S = G + C, W = A + T, H = A + T + C, B = G + T + C, D = G + A + T, N = A + C + G + T, V = G + A +C. Phosphorothioate linkages=*, 2'O-Methyl RNA = [mA], [mG], [mC], [mU], LNA Bases= [+A], [+G], [+C], [+T], DNA Bases within RNA oligos = [dA], [dC], [dG], [dT] QM-FM-028, Version 2, Effective: 6/24/2010. Sigma and Sigma Aldrich are registered trademarks of Sigma-Aldrich Biotechnology, L.P. and Sigma-Aldrich Co.