test• 1. Alanine scan means:
– A. sequential substitutions (mutagenesis) of amino acids by alanine?
– B. sequential substitutions (mutagenesis) of alanine residues by cysteins?
– C. searching for alanine residues in protein sequnces?
– D. specific „scanning“ of proteomic data?
• 2. Classical yeast two-hybrid system is based on:
– A. reactivation of GFP fluorescence?
– B. reactivation of transcription activator (like Gal4)?
– C. reactivation of DHFR enzyme?
– D. reactivation of signaling RAS pathway?
• 3. Site directed „crosslink“ by BMOE:
– A. covalently links SH groups of cystein residues?
– B. covalently links SH groups of lysine residues?
– C. covalently links NH2 groups of cystein residues?
– D. links SH groups of cystein residues non-covalently?
• 4. FRET method is based on:
– A. reactivation of GFP fluorescence?
– B. reactivation of transcription activator (like Gal4)?
– C. fluorescence resonance of ethan?
– D. proximity of fluorophore (e.g. CFP) emitting light with a wavelength absorbed by the other fluorophore
(e.g. YFP) which then emits detected light?
Send the test to E-mail: jpalecek@sci.muni.cz