SALES ORDER NO: 8820007824 CUSTOMER NO: 0000002011 SHIPMENT DATE: 03/03/2023 Technical Datasheet INSTITUTE: Prírodovedecká fakulta MU RESEARCHER: Prírodovedecká fakulta MU PURCHASE ORDER NO: 3112/0070/23 Batch # Oligo Name Oligo # Len Pur Scale MW Tm° ug/OD OD nmol Epsilon 1/(mMcm) Dime 2ndry GC% |il for 100uM* Sequence(5'-3') HA16369766 T7-G02-HIS_FW 8820007824-30/0 32 DST 0.025 9739 84.7 32.3 9.4 304.2 31.2 300.9 No Strong 59.3 312 TCGCGGATCCCTGCCACACTACATCAGGAGCA HA16369767 HIST7-G02_FW 8820007824-40/0 32 DST 0.025 9739 84.7 32.3 9.56 309.4 31.7 300.9 No Strong 59.3 317 TCGCGGATCCCTGCCACACTACATCAGGAGCA HA16369768 HIS-G02_FW 8820007824-50/0 32 DST 0.025 9777 76.8 31.4 8.52 267.6 27.3 311.2 No Moderate 46.8 273 TATGCATATGCTGCCACACTACATCAGGAGCA HA16369769 G02-HIS_FtEV 8820007824-60/0 33 DST 0.025 10154 80.8 32.1 9.16 294.1 28.9 316.2 No Moderate 51.5 289 GGTGCTCGAGCTGCATGGAAGCATAATCTTCCA HA16369770 G02_FtEV 8820007824-70/0 34 DST 0.025 10458 78.5 32.2 7.04 226.7 21.6 324.7 No Moderate 50 216 GGTGCTCGAGTCACTGCATGGAAGCATAATCTTC HA16369771 tHSP_FW 8820007824-80/0 39 DST 0.025 12142 72.1 29.9 11.72 351.1 28.9 405.3 No Weak 33.3 289 GAGCTCAAGCTTATATGAAGATGAAGATGAAATATTTGG HA16369772 tHSP_REV 8820007824-90/0 41 DST 0.025 12482 66.5 31.2 5.4 168.5 13.5 399.8 No Weak 26.8 135 ACTGATAGTTTAAACTTATCTTTAATCATATTCCATAGTCC * Calculation is provided lor oligos shipped dry. Centrifuge tube prior to opening to prevent loss of pelleted oligonucleotide. For Research Use Only Key to Symbols: R = A + G, Y = C + T, M = A + C, K = G + T, S = G + C, W=A+T, H = A+ T+C, B = G + T + C, D = G + A+T, N = A+ C + G + T, V = G + A +C. Phosphorothioate linkages-*, 2'O-Methyl RNA = [mA], [mG], [mC], [mU], LNA Bases= [+A], [+G], [+C], [+T], DNA Bases within RNA oligos = [dA], [dC], [dG], [dT] Bin Number: 223 QM-FM-028, Version 2, Ettective: 6/24/2010. Sigma and Sigma Aldrich are registered trademarks ot Sigma-Aldrich Biotechnology, L.P. and Sigma-Aldrich Co. StGMA Where bio begins lift íťr'imp