Cell Density
Monitoring System
Operating Instructions
3Cell Density Monitoring System Operating Instructions
TABLE OF CONTENTS
Hamilton Warranty
Please refer to the General Terms of Sales (GTS).
Important note
Copyright © 2021 Hamilton Bonaduz AG, Bonaduz Switzerland. All rights reserved. The
reproduction of any part of this document in any form is forbidden without the express
written agreement of Hamilton Bonaduz AG.
Contents of this operating instruction can be modified without previous announcement.
Technical modifications reserved. Greatest possible care was used on the correctness
of the information in this operating instruction. If errors should be discovered nevertheless,
Hamilton Bonaduz AG is pleased to be informed about it. Regardless of this, Hamilton
Bonaduz AG cannot assume liability for any errors in this operating instruction or for
their consequences.
Table of Contents
1	Preface..................................................................................................................10
2	 General Information.............................................................................................10
2.1	 Intended Use.............................................................................................................. 10
2.2	 About this Operating Instruction................................................................................ 10
3	Liability.................................................................................................................. 11
4	 Safety Precautions and Hazards........................................................................ 11
4.1	 General Precautions....................................................................................................11
4.2	 Operation of the Cell Density Monitoring System....................................................... 12
4.3	 Electrical Safety Precautions...................................................................................... 12
4.4	 Chemical, Radioactive or Biological Hazard Precautions.......................................... 14
5	 Product Description............................................................................................. 14
5.1	 Why Measuring Cell Density?.................................................................................... 14
5.2	 The Theory of Permittivity Measurement (Incyte)....................................................... 15
5.2.1	 The Dual-Frequency Measurement Mode.................................................... 15
5.2.2	 The Frequency Scan Mode........................................................................... 16
5.3	 The Theory of Turbidity Measurement........................................................................17
5.4	 Hardware Description................................................................................................ 18
5.4.1	 Control Unit................................................................................................... 20
5.4.1.1	 Arc View Controller..................................................................... 20
5.4.1.2	ComBox..................................................................................... 21
5.4.1.3	 Cell Density Integration Kit......................................................... 22
5.4.2	 Incyte Sensor Unit......................................................................................... 23
5.4.3	 Dencytee Sensor Unit................................................................................... 24
5.5	 Outline of the Control Unit Software........................................................................... 25
5.5.1	 Run Menu..................................................................................................... 25
5.5.2	 Sensor Channel Tab...................................................................................... 28
5.5.3	 Main Menu.................................................................................................... 28
5.5.4	 Unit Settings Menu........................................................................................ 30
5Cell Density Monitoring System Operating Instructions
5.5.5	 Measure Settings Menu................................................................................ 31
5.5.6	 Incyte Frequency Scan Settings Menu......................................................... 32
5.5.7	 Culture Settings Menu.................................................................................. 33
6	Installation............................................................................................................34
6.1	 Install the Control Unit................................................................................................ 34
6.1.1	 Install the Arc View Controller........................................................................ 34
6.1.2	 Install the ComBox........................................................................................ 35
6.1.3	 Install the Cell Density Integration Kit............................................................ 36
6.2	 Mount the Sensor in the Bioreactor........................................................................... 37
6.3	 Connect the Sensor Unit to the Control Unit.............................................................. 40
7	 Operation of Incyte..............................................................................................42
7.1	 Choose the Measurement Mode............................................................................... 42
7.2	 Addition of Arc Sensors............................................................................................. 42
7.3	 Start Recording Measurements in a Culture File ....................................................... 44
7.4	 Perform a Product Calibration and Set the Inoculation Time..................................... 45
7.5	 Add a Comment to the Culture File............................................................................ 45
7.6	 Customize the Main Graph........................................................................................ 46
7.7	 Setup a Multi View Graph.......................................................................................... 47
7.8	 Stop Recording the Culture File ................................................................................ 48
7.9	 Dismount the Sensor Unit ......................................................................................... 48
7.10	 Export a Culture File................................................................................................... 49
7.11	 Import a Culture File in an Excel Spreadsheet........................................................... 50
7.12	 Further Measurement Settings.................................................................................. 51
7.12.1	 Set the Cell Factor...................................................................................... 51
7.12.2	 Start the Incyte Scan................................................................................. 51
7.12.3	 Define the Custom Measurement Mode.................................................... 52
7.12.4	 Perform a Manual Product Calibration....................................................... 53
7.13	 Use Sensor Cleaning (Probe Cleaning)...................................................................... 53
8	 Operation of Dencytee.........................................................................................55
8.1	 Choose the Measurement Mode............................................................................... 55
8.2	 Addition of Arc Sensors............................................................................................. 55
8.3	 Start Recording Measurements in a Culture File........................................................ 58
8.4	 Perform Product Calibration and Set the Inoculation Time........................................ 59
8.5	 Add a Comment to the Culture File............................................................................ 59
TABLE OF CONTENTS
8.6	 Customize the Main Graph ....................................................................................... 60
8.7	 Setup a Multi View Graph.......................................................................................... 61
8.8	 Stop Recording the Culture File ................................................................................ 62
8.9	 Dismount the Sensor Unit ......................................................................................... 62
8.10	 Export a Culture File................................................................................................... 62
8.11	 Import the Culture File in an Excel Spreadsheet........................................................ 63
8.12	 Define the Custom Measurement Mode.................................................................... 64
9	 Administrator Menu.............................................................................................65
9.1	 Definition of the User Level......................................................................................... 66
9.1.1	 Change the Administrator Password............................................................ 66
9.1.2	 Define the User Level.................................................................................... 67
9.2	 Adjust the General Settings....................................................................................... 67
9.2.1	 Touch Screen Calibration.............................................................................. 68
9.2.2	 Adjustment of the Decimal Separator in the Culture File............................... 68
9.2.3	 Continuous Display of Graphed Data............................................................ 68
9.2.4	 Adjustment of Date and Time Settings.......................................................... 68
9.2.5	 Initialize the Arc View M12 Connectors......................................................... 69
9.2.6	 Enable the Cell Density Integration Kit Option............................................... 69
9.3	 Read the Network Settings........................................................................................ 70
9.4	 Adjust the Network Applications................................................................................ 71
9.4.1	 Activate Share Data Files............................................................................... 72
9.4.2	 Activate OPC Communication...................................................................... 72
9.5	 Install a License.......................................................................................................... 72
9.6	 Transfer Administrator Files........................................................................................ 73
9.6.1	 Export the Log Files.......................................................................................74
9.6.2	 Erase Temporary Files....................................................................................74
9.6.3	 Export Culture Data.......................................................................................74
9.7	 Update or Re-Set the Arc View Controller or Cell Density Integration Kit.................. 75
9.7.1	 Software Update of the Control Unit............................................................. 75
9.7.1.1	 Software Update of the Arc View Controller or
	 Cell Density Integration Kit......................................................... 75
9.7.1.2	 Software installation/update of the ComBox.............................. 76
	 9.7.1.2.1	 Procedure for one ComBox per PC.................... 76
	 9.7.1.2.2	 Procedure for two ComBoxes per PC................ 76
9.7.2	 Re-Set the Arc View Controller or Cell Density Integration Kit..................... 77
9.7.3	 Update Recovery of the Arc View Controller................................................. 77
TABLE OF CONTENTS
7Cell Density Monitoring System Operating Instructions
10	Maintenance.........................................................................................................78
10.1	 Daily Maintenance...................................................................................................... 78
10.2	 Weekly Maintenance.................................................................................................. 78
10.3	 Manual Cleaning Procedure at Process End for Cell Density Sensors...................... 79
10.3.1	 Incyte Sensors........................................................................................... 79
10.3.2	 Dencytee Sensors...................................................................................... 79
10.4	 Yearly Maintenance.................................................................................................... 79
10.5	 Sensor Unit Verification.............................................................................................. 80
10.5.1	 Verification of an Incyte Sensor Unit........................................................... 80
10.5.2	 Verification of a Dencytee Sensor Unit....................................................... 81
10.6	 Calibration of a Sensor Unit....................................................................................... 82
10.6.1	 Calibrate an Incyte Sensor Unit.................................................................. 83
10.6.2	 Calibrate a Dencytee Sensor Unit.............................................................. 86
10.7	 Transfer a Calibration Files to the Arc View Controller................................................ 86
10.7.1	 Clean and Back Up the Arc View Controller.............................................. 87
10.7.2	 Update the Control Unit............................................................................. 87
11	Troubleshooting....................................................................................................88
11.1	 Self-Diagnostic of the Sensor Unit............................................................................. 88
11.2	 Re-Set the Factory Settings....................................................................................... 89
11.3	 Verification and Calibration Error................................................................................ 89
11.3.1	 Verification Error......................................................................................... 89
11.3.2	 Calibration Error......................................................................................... 89
11.4	 Getting Technical Support.........................................................................................90
11.4.1	 Create Print Screens..................................................................................90
11.4.2	 System Information....................................................................................90
11.4.3	 System Data............................................................................................... 91
11.5	 Remove Corrupt Files................................................................................................ 91
11.6	 Transfer Error-Log-Files from the Arc View Controller................................................ 92
11.7	 Returning a System for Repair................................................................................... 93
12	 Connection of the Control Unit to the Process Control System......................94
12.1	 Connect by Modbus communication......................................................................... 94
12.1.1	 Preparation of the Control Unit................................................................... 94
12.1.2	 Preparation of the Modbus Connection..................................................... 95
12.1.2.1	 Definition of the Return Time...................................................... 95
12.1.2.2	 Overview of the Available Data................................................... 96
TABLE OF CONTENTSTABLE OF CONTENTS
12.1.2.3	 Definition of 32 Bit Data Type..................................................... 97
12.1.2.4	 Definition of Modbus Exceptions................................................ 98
12.1.2.5	 Pre-Amplifier Status Codes........................................................ 98
12.1.2.6	 Calibration and Validation via Modbus..................................... 100
	 12.1.2.6.1	 General Concepts............................................. 100
	 12.1.2.6.1.1	 Controlling the State Machine via Modbus....... 100
	 12.1.2.6.1.2	 The main state machine.................................... 102
	 12.1.2.6.1.3	 Modbus registers for CDIK, Arc View
		 Controller 265 and PC software....................... 103
	 12.1.2.6.1.4	 Modbus registers for Arc View Controller 465.. 104
	 12.1.2.6.1.5	 General error states.......................................... 105
	 12.1.2.6.2	 Validation Incyte Unit......................................... 106
	 12.1.2.6.2.1	 Table of states................................................... 107
	 12.1.2.6.2.2	 Error states....................................................... 107
	 12.1.2.6.3	 Calibration Incyte Unit....................................... 108
	 12.1.2.6.3.1	 Table of states................................................... 109
	 12.1.2.6.3.2	 Dilution steps for different calibration types.......110
	 12.1.2.6.3.3	 Error states........................................................111
	 12.1.2.6.4	 Validation Dencytee...........................................112
	 12.1.2.6.4.1	 Table of states....................................................113
	 12.1.2.6.4.2	 Error states........................................................113
	 12.1.2.6.5	 Calibration Dencytee..........................................114
	 12.1.2.6.5.1	 Table of states....................................................115
	 12.1.2.6.5.2	 Error states........................................................115
12.1.2.7	 Modbus Tables Arc View Controller 265, ComBox and
	 Cell Density Integration Kit........................................................116
	 12.1.2.7.1	 Input Registers Tables (Read only).....................116
	 12.1.2.7.1.1	 Read the Modbus Commands..........................116
	 12.1.2.7.1.2	 Incyte Input Registers Table...............................116
	 12.1.2.7.1.3	 Dencytee Input Registers Table........................ 122
	 12.1.2.7.2	 Holding Registers (read and write).................... 123
	 12.1.2.7.2.1	 Read and Write Function Code ........................ 123
	 12.1.2.7.2.2	 Incyte Holding Registers Table...........................124
	 12.1.2.7.2.3	 Dencytee Holding Registers Table.................... 125
	 12.1.2.7.2.4	 Allowed Values for Holding Registers................127
12.1.2.8	 Modbus Tables Arc View Controller 465 (XL) ...........................127
	 12.1.2.8.1	 Input Registers Tables (Read only).....................127
9Cell Density Monitoring System Operating Instructions
	 12.1.2.8.1.1	 Read the Modbus Commands..........................127
	 12.1.2.8.1.2	 Incyte Input Registers Table.............................. 128
	 12.1.2.8.1.3	 Dencytee Input Registers Table........................ 139
	 12.1.2.8.2	 Holding Registers (read and write) ....................142
	 12.1.2.8.2.1	 Read and Write Function Code .........................142
	 12.1.2.8.2.2	 Incyte Holding Registers Table.......................... 143
	 12.1.2.8.2.3	 Dencytee Holding Registers Table.................... 145
	 12.1.2.8.2.4	 Allowed Values for Holding Registers................147
12.2	 Connect by OPC Communication........................................................................... 148
12.2.1	 Introduction to OPC................................................................................. 148
12.2.2	 Configuration of the Control Unit.............................................................. 148
12.2.3	 Configuration of the OPC Client............................................................... 150
12.2.4	 OPC Classic Demo Client Start Guide..................................................... 150
12.2.4.1	 System Overview..................................................................... 150
12.2.4.2	 Download and Installation.........................................................151
12.2.4.3	 Connecting the OPC Classic Demo Client to the Control Unit....151
12.2.5	 Softing OPC Bridge Start Guide.............................................................. 155
12.2.5.1	 System Overview..................................................................... 155
12.2.5.2	 Download and Installation........................................................ 155
12.3	 Connecting the 4-20 mA Output Box...................................................................... 163
12.3.1	 Electrical Installation................................................................................. 163
12.3.2	 Software Configuration............................................................................ 166
12.4	 Connect by Profibus Communication...................................................................... 167
12.4.1	 Profibus General Information.................................................................... 167
12.4.1.1	 Ground the Modbus-Profibus module..................................... 168
12.4.2	 Profibus Interface..................................................................................... 168
12.4.2.1	 Overview.................................................................................. 168
12.4.2.2	 Input Buffer.............................................................................. 169
12.4.2.3	 Output Buffer........................................................................... 169
12.4.2.4	 Decoding the Return Values......................................................172
12.4.2.5	 Function codes..........................................................................174
12.4.3	 Modbus Interface..................................................................................... 182
12.4.3.1	 Configuration............................................................................ 182
13	Disposal..............................................................................................................182
14	 Software Versions..............................................................................................183
15	 Hardware Compatibility.....................................................................................184
16	 Ordering Information..........................................................................................184
16.1	Controller................................................................................................................. 184
16.2	 Cell Density Integration Kit....................................................................................... 185
16.3	Units......................................................................................................................... 185
16.4	 Spare Parts and Accessories................................................................................... 186
16.5	 Calibration Tools....................................................................................................... 187
17	Glossary..............................................................................................................187
18	FAQ......................................................................................................................188
18.1	 General Questions................................................................................................... 188
18.2	 Incyte Questions...................................................................................................... 190
TABLE OF CONTENTSTABLE OF CONTENTS
11Cell Density Monitoring System Operating Instructions
LIABILITY
SAFETY PRECAUTIONS AND HAZARDS
1	Preface
Welcome to the World of Hamilton Precision Instruments.
Congratulations on your purchase of a Hamilton Cell Density Monitoring System comprising
a Control Unit (Arc View Controller, Cell Density ComBox, Cell Density Integration Kit) and an
Incyte and/or Dencytee Unit. The sensors are designed for monitoring permittivity (Incyte) and
turbidity (Dencytee). This technology enables the online monitoring of viable and total cell density
during biotechnological processes. For the first time you can monitor cells in real-time, obtain
actionable data, and automate process adjustment. A standard measuring loop consists of a
Sensor Unit (i.e. a sensor with a Pre-amplifier), which is connected to the Control Unit. With the
Control Unit, it is also possible to view Arc sensors wirelessly, like pH and DO, on just one display.
Proper handling and maintenance of this monitoring technology will increase the lifespan of the
system. To learn about proper care and maintenance, please take the time to read this manual,
including the warranty information.
2	 General Information
2.1	 Intended Use
The Cell Density Monitoring System is designed to measure permittivity with the Incyte sensor
and turbidity with the Dencytee sensor in a liquid medium. These measurements may be
used for the control of bioprocesses within the defined specifications (see specifications sheets
www.hamiltoncompany.com). The permittivity measurement may be correlated to the viable cell
density, and the turbidity measurement may be correlated to the total cell density. In addition
to permittivity Incyte also measures conductivity. This measurement should not be used for the
control of bioprocesses.
2.2	 About this Operating Instruction
These Operating Instructions will help users to operate the Cell Density Monitoring System
correctly and safely. To achieve that goal, this document describes the different components
of the system and how they function. The Operating Instructions describe both the hardware
and software of the Cell Density Monitoring System in a depth enabling the user to operate the
system. After introducing the various parts, it is shown step by step how to operate the system.
After reading the Operating Instructions, users should be capable of installing and operating the
Cell Density Monitoring System. Following information are highlighted within this document:
PREFACE • GENERAL INFORMATION
ATTENTION! Essential information for avoiding personal injury or damage to
the equipment.
NOTE: Important instructions or interesting information.
3	Liability
The liability of Hamilton Bonaduz AG is detailed in the document “General Terms and Conditions
of Sale and Delivery,” Hamilton is expressly not liable for direct or indirect losses arising
from the use of the sensors. It must in particular be insured in this conjunction that malfunctions
can occur on account of the inherently limited useful life of sensors contingent upon
their relevant applications. The user is responsible for the calibration, maintenance and regular
replacement of the sensors. In the case of critical sensor applications, Hamilton recommends
using back-up measuring points in order to avoid consequential damages. The user is
responsible for taking suitable precautions in the event of a product failure. The sensor is not
intended as a safety device.
4	 Safety Precautions and Hazards
ATTENTION! Read the following safety instructions carefully before installing
and operating the Cell Density Monitoring System.
4.1	 General Precautions
For safe and correct use of Incyte and Dencytee, it is essential that both operating and service
personnel follow generally accepted safety procedures as well as the safety instructions given in
this document, the operating instruction of the Cell Density Monitoring System. The specification
given (see specifications sheets www.hamiltoncompany.com) as regards temperature; pressure
etc. may under no circumstances be exceeded. Inappropriate use or misuse can be dangerous.
Cleaning, assembly and maintenance should be performed by personnel trained in such work.
13Cell Density Monitoring System Operating Instructions
Before removing the sensor from the measuring setup, always make sure that no process medium
can be accidentally spilled. When removing and cleaning the sensor, it is recommended to wear
safety goggles and protective gloves. If the system cannot be repaired by the operator, it has to
be sent back to Hamilton for inspection. Necessary precautions should be taken when transporting
the sensors. For repair or shipment the System should be sent back in the original reusable
packaging box. Every Incyte or Dencytee sensor sent back for repair must be decontaminated. If
the conditions described in these operating instructions are not adhered to or if there is any inappropriate
interference with the equipment, all of our manufacturer’s warranties become obsolete.
4.2	 Operation of the Cell Density Monitoring System
The Cell Density Monitoring System must be used for the intended applications, and in optimum
safety and operational conditions. The specifications (such as temperature or pressure) defined
in the section entitled ‘Technical Specification’ (Chapter 15) must not be exceeded under any
circumstances.
Make sure that the process connections and O-rings are not damaged when screwing a sensor
into the process. O-rings are consumable parts which must be exchanged regularly (at least
once per year). Even when all required safety measures have been complied with, potential
risks still exist with respect to leaks or mechanical damage to the armature. Wherever there are
seals or screws, gases or liquids may leak out undetected. Always make sure that no process
medium can be accidentally spilled before removing the sensor from its measurement set up.
Do not put stress on the system by vibration, bending or torsion.
You may leave the pre-amplifier connected to the sensor during cleaning in place (CIP) and
sterilization in place (SIP). Stand clear of the pre-amplifier during CIP and SIP procedures as it
may become very hot. The pre-amplifier is not autoclavable. Dismount it prior to autoclavation to
avoid damages to the electronics.
4.3	 Electrical Safety Precautions
Only use the power supply provided with the Arc View Controller, or the ComBox. Do not connect
it to a power source of any voltage beyond the range stated in the specifications. Failure to
do so may lead to malfunction or damage of the system or impair user safety.
ATTENTION! Disconnect from power the Arc View Controller and ComBox prior
to mounting and dismounting a Sensor Unit.
In areas having significant electronic noise, Incyte sensors and preamps may require grounding.
Locations having electronic noise may cause interference to your permittivity readings. Grounding
the Incyte sensor and preamp should alleviate the interference.
•	 If the headplate of a benchtop bioreactor is not grounded, connect an earth ground wire to it.
•	 For large metallic bioreactors it is not necessary to ground the Incyte sensor or preamp if
the bioreactor is already grounded.
•	 For large bioreactors without a ground (e.g. glass lined reactors) connect a ground wire to
the fitting the Incyte sensor is mounted to.
ATTENTION! Only use
the Hamilton M 12 / M 12
(5 pole) cable to connect
Control and Sensor Unit.
Connect the headplate to ground.
The sensor and preamp will be grounded
via its stainless steel PG13.5 thread.
The stainless steel
PG13.5 thread
connects the
sensor and preamp
to ground.
	
Connect the fitting
to ground. The
sensor and preamp
will be grounded via
its stainless steel
PG13.5 thread.
The Tank is grounded.	 The Tank is not grounded.
Figure 1: Grounding
SAFETY PRECAUTIONS AND HAZARDSSAFETY PRECAUTIONS AND HAZARDS
15Cell Density Monitoring System Operating Instructions
4.4	 Chemical, Radioactive or Biological Hazard Precautions
Selection of the appropriate biological safety level and implementation of the required biosafety
measures for working with the Cell Density Monitoring System is the sole responsibility of the user.
If working with hazardous liquids observe and carry out the maintenance procedures, paying
attention to cleaning and decontamination. If parts or the complete Cell Density Monitoring
System become contaminated with biohazardous, radioactive or chemical material, it should be
cleaned. Failure to observe and carry out the maintenance procedures may impair the reliability
and correction functioning of the system.
Avoid damaging the power cord. Do not bend it excessively, step on it, or place heavy objects
on it. A damaged cord can easily become a shock or fire hazard. Never use a power cord after
it has become damaged.
5	 Product Description
5.1	 Why Measuring Cell Density?
Continuous monitoring of bioprocesses is required for both process control and optimization.
The control of the environmental conditions including pH or dissolved oxygen is well established
but does not provide information on the cell physiology. Parameters relating to cell physiology
are usually monitored offline after daily sampling of the culture. This method is time-consuming
and provides only discrete information on the bioprocess. Incyte and Dencytee sensors offer an
alternative for directly monitoring viable and total cell density in real time.
The Incyte sensor enables real-time, and online measurement of permittivity, which correlates
with the viable cell density. The measurement is not influenced by changes in the media, or by
the presence of microcarriers, dead cells, and cellular debris. The Incyte sensor has been especially
designed for monitoring the culture of mammalian and insect cells. It may also be used for
controlling yeast culture and high-density bacterial fermentation. Online monitoring of permittivity
with Incyte enables the early detection of process deviations without sampling and supports
timely process adjustment.
The Dencytee sensor allows the real-time, and online measurement of turbidity, which correlates
with the total cell density. The turbidity of a cell suspension refers to the optical density of the cell
suspension measured at a wavelength of 880 nm. Monitoring total cell density with Dencytee
PRODUCT DESCRIPTIONPRODUCT DESCRIPTION
is particularly suitable after inoculation when cells are expanding quickly but concentrations are
low, making capacitance-based readings less reliable. The turbidity measurement is performed
in the infra-red range and is therefore insensitive to changes in media color.
Used in combination Incyte and Dencytee provide a complete and real-time characterization of
the cell population for better bioprocess control.
5.2	 The Theory of Permittivity Measurement (Incyte)
5.2.1	 The Dual-Frequency Measurement Mode
In an alternating electrical field viable cells behave like small capacitors, but not dead cells or
cellular debris (Figure 1A). The charge of these small capacitors is measured by the Incyte
sensor and reported as permittivity in pF/cm.
The permittivity of viable cells is measured at a frequency specific of the cell type (fmes): usually
1 MHz for mammalian cells and bacteria, and 2 MHz for yeast (Figure 1B). It is continuously and
automatically corrected for the background permittivity measured at high frequency (fhigh). This
is the standard Dual-Frequency Measurement Mode. The permittivity measured by Incyte is
specific to viable cells and correlates with the viable cell density, especially during the exponential
growth phase.
ELECTRODES
ELECTRIC FIELD
VIABLE CELLS POLARIZE
DEAD CELLS HAVE A
DAMAGED MEMBRANE AND
DO NOT POLARIZE
A
+
+
+
+
+
+
+
+
+
- - -
-
-
-
-
-
Figure
1: Incyte measurement principle.
A: Viable but not dead cells exhibit a permittivity signal.
17Cell Density Monitoring System Operating Instructions
PRODUCT DESCRIPTIONPRODUCT DESCRIPTION
B: In the dual measurement mode the permittivity of viable cells is measured at a frequency specific to
the cell type (fmes) and corrected by the background permittivity measured at high frequency (fhigh).
5.2.2	 The Frequency Scan Mode
The polarization behavior of cells varies strongly at different frequencies, as shown in Figure 1B.
Cells fully polarize at low frequency whereas they hardly polarize at high frequency. The Incyte
Scan measures the permittivity signal at 17 different frequencies between 0.3 and 10 MHz
(Figure 2) and provides additional information especially on the cells physiology.
permittivity[pF/cm]
0.3 1 20
Frequency [MHz] Medium Permittivity
High frequency plateau
Figure 2: The ideal frequency spectrum of mammalian cells, called beta-dispersion.
permittivity[pF/cm]
0.1 1 20fmeas fhigh
Frequency [MHz]
2
3
2
3
3
Medium Permittivity
1
OpOptitimamall
frequency
for correlation
of permittivity
and viable
cell density
Low frequency plateau
Permittivity
of cells and
medium High frequency plateau
3
= -
3
1
B The analysis of the Incyte Scan provides additional information on the cell physiology1
. During
the Incyte Scan the most relevant parameters - Δεmax, fc, and α - are automatically calculated
and displayed on the Arc View Controller (Figure 3). The characteristic frequency, fc, provides an
indication of the average cell diameter. If fc decreases, it indicates that the cell diameter increases
during the culture. On the contrary, a shift of fc towards the higher frequency range indicates
that the cell size reduces. The height of the low frequency plateau, Δεmax, correlates with the
viable cell density. It increases as the cells grow. The slope (α) of the beta-dispersion at the
characteristic frequency fc provides an indication of the distribution of the cell diameter. A steep
slope, i.e. a large α indicates an homogenous culture.
permittivity[pF/cm]
0.3 1.0 20
fcFF
frequency [MHz]
3
Figure 3: Data Interpretation of the beta-dispersion, gained from an Incyte Scan.
5.3	 The Theory of Turbidity Measurement
Turbidity is commonly used to estimate the concentration of a cell suspension, especially in microbiology.
The Dencytee sensor provides online measurement of the turbidity of a cell suspension,
based on optical density (Figure 4). This measurement correlates with the total cell density
in a bioprocess. The sensor emits light through a 5 mm window onto a light detector. Cells in
suspension absorb and scatter light so less light is read by the detector. The measurement is
made at NIR (near infra-red) wavelengths so it is insensitive to changes in media color. All particles
that scatter light at 880 nm will be detected, including living and dead cells.
1
Ansorge S, Esteban G, Schmid G. On-line monitoring of infected Sf-9 insect cell cultures by scanning permittivity measurements and
comparison with off- line biovolume measurements. Cytotechnology. 2007;55(2-3):115-124. doi:10.1007/s10616-007-9093-0.
19Cell Density Monitoring System Operating Instructions
PRODUCT DESCRIPTIONPRODUCT DESCRIPTION
Figure 4: Measurement principle of Dencytee sensors.
5.4	 Hardware Description
A standard measuring loop consists of a Sensor Unit connected to the Control Unit (Figure 5).
It is recommended to measure in parallel turbidity with Dencytee and permittivity with Incyte in
order to collect information on both total and viable cell density.
LIGHT SOURCE, EMITS AT 880 NM
DETECTOR
5 MM WINDOW
Figure 5: Overview of the Cell Density Monitoring System (A: Arc View Controller, B: ComBox,
C: Cell Density Integration Kit), connected to a process control system (PCS).
PCS
PC
4-20 mA OUTPUT BOX
MODBUS
DENCYTEE
UNIT
INCYTE UNIT
DN 12
B
OPC*
COMBOX
PCS INDUSTRIAL PC
4-20 mA OUTPUT BOX
MODBUS
DENCYTEE
UNIT
INCYTE UNIT
DN 12
C
OPC
COMBOX
CELL DENSITY INTEGRATION KITSKID CONTROLLER
*By Modbus-Profibus DP Module, only for Arc View 465 (XL)
*In order to enable a OPC connection between a Combox and
the PCS via OPC only one Box can be installed per PC.
VCD
TCD
PCS ARC VIEW CONTROLLER
4-20 mA OUTPUT BOX
MODBUS / PROFIBUS*
DENCYTEE
UNIT
INCYTE UNIT
DN 12
A
OPC
21Cell Density Monitoring System Operating Instructions
PRODUCT DESCRIPTIONPRODUCT DESCRIPTION
5.4.1	 Control Unit
5.4.1.1	 Arc View Controller
The Arc View Controller (Figure 6) allows recording and plotting the measurements of up to four
connected Sensor Units. It stores the measurements in Culture Files and the calibration data of
all Sensor Units that have been connected. Hamilton Arc sensors, especially pH and dissolved
oxygen sensors, may be visualized and recorded in parallel via a wireless connection. Most connection
ports are available on the right hand side panel (Figure 6). The Arc View Controller 265
holds two M12 plugs on the side panel for the connection of two Sensor Units. They are labeled
as Channel. The Arc View Controller 465 is a four channel Controller and has two more M12
plugs for connecting additional Sensor Units. The AUX and Modbus M12 connectors, and the
Ethernet plug may be used to connect the Arc View Controller to the process control system.
The USB ports may be used to connect a USB stick and export the recorded log files. It also
serves to connect the Arc Wireless Converter MB (Ref 243498) or BT (Ref 243499) required to
log Arc sensors.
Figure 6: Arc View Controller 265, front and side panel.
VCD
TCD
ON/OFF
Channel 1 Channel 2
ModbusAUX
DC 24V
MOUNTING HOLES
MAIN DISPLAY
ON / OFF KEY SWITCH
POWER SUPPLY
M12 CONNECTORS
TO SENSOR
M12 CONNECTOR
TO ANALOG BOX
M12 CONNECTOR
TO MODBUS RTU
AND MODBUSPROFIBUS
DP
MODULE
USB PORT
RJ45 ETHERNET
CONNECTOR
ATTENTION! Instead of using the key switch it is recommended to plug/unplug
the Arc View Controller, if the serial number is below 1500/5500.
5.4.1.2	ComBox
The ComBox (Figure 7) allows recording and plotting the measurements of up to two connected
Sensor Units on a personal computer. The measurements in Culture Files and the
calibration data of all Sensor Units that have been connected are recorded on the PC. Hamilton
Arc sensors, especially pH and dissolved oxygen sensors, may be visualized and recorded
in parallel via a wireless connection, the Arc Wireless Converter MB (Ref 243498) or BT (Ref
243499) has to be connected to an USB Port.
The connection ports are available on top of the ComBox (Figure 7). It holds two M12 plugs for
the connection of two Sensor Units. They are labeled as Channel. The AUX and Modbus M12
connectors may be used to connect the ComBox to the process control system.
Figure 7: ComBox, top panel
ModbusAUXChannel 2Channel 1
MOUNTINGHOLES
(onlyCellDensityIntegrationKit)
POWER SUPPLY
(not for Cell Density Integration Kit)
M12 CONNECTORS
TO SENSOR
MOUNTINGHOLES
(onlyCellDensityIntegrationKit)
M12 CONNECTOR
TO ANALOG BOX
M12 CONNECTOR TO MODBUS RTU
AND MODBUS-PROFIBUS DP MODULE
23Cell Density Monitoring System Operating Instructions
PRODUCT DESCRIPTIONPRODUCT DESCRIPTION
5.4.2	 Incyte Sensor Unit
An Incyte Sensor Unit consists of a Pre-amplifier and a Sensor (Figure 9). The Pre-amplifier and
the Sensor have been factory calibrated as a pair. They are connected together via a VP11 connector.
A status LED, indicating whether the sensor is ready for measurement is available on top of
the Incyte pre-amplifier. The Incyte Sensor Unit is available with a shaft diameter of 12 mm (DN12)
or 25 mm (DN25). The measurement tip is made of PEEK and contains four platinum electrodes
for permittivity measurement. All wetted parts are USP Class VI certified.
Figure 9: Incyte Sensor Unit assembled.
STATUS LED
 M12 CONNECTOR
VP11 CONNECTOR
PEEK TIP WITH
PLATINUM ELECTRODES
PRE-AMPLIFIERSENSOR
INCYTESENSORUNIT
5.4.1.3	 Cell Density Integration Kit
The Cell Density Integration Kit (Figure 8) allows the complete integration of Incyte and Dencytee
sensors into the process control system either by Modbus, OPC or 4-20 mA.
The kit comprises an industrial PC, to run the required Software and a ComBox, to communicate
to the Sensor Units and the process control system. The connection ports are available on
top of the ComBox (Figure 7). It holds two M12 plugs for the connection of two Sensor Units.
They are labeled as Channel. The AUX and Modbus M12 connectors may be used to connect
the ComBox to the process control system.
Figure 8: Cell Density Integration Kit comprising the industrial PC and ComBox
NOTE: The connector is specified for 8 A and accepts a cable cross-section upto
1,5 mm2
.
12
34
LAN 1
LAN 2
MOUNTINGHOLES
POWER SUPPLY
USB PORT
RJ45 ETHERNET
CONNECTOR
DVI-I PORT
P-S (POWER-STATE EXIT)
PC_ON (PC_ON INLET)
GND (OV)
24V + (POWER SUPPLY)
25Cell Density Monitoring System Operating Instructions
PRODUCT DESCRIPTIONPRODUCT DESCRIPTION
5.5	 Outline of the Control Unit Software
5.5.1	 Run Menu
The Run Menu (Figure 11) appears automatically upon starting the Arc View Controller or
ComBox. It provides all the relevant information on a sensor connected to one of the Control
Unit Channels. The sensor status is shown as a status LED in the Channel Tab: The Main Graph
shows the measurements of the sensor connected to the selected channel over time. The measurement
values are also indicated on top of the Main Graph.
The Run Menu provides access to additional menus.
•	 The Main Menu is accessible with the Menu Button located on the bottom right hand side
of the screen.
•	 Four additional Settings Menus (Unit, Measure, F-Scan and Culture) are available using the
buttons located on the left hand side of the screen.
•	 The Graph Arc Settings Menu is used to start recording additional Arc sensors usually
pH or dissolved oxygen.
•	 The Multi-View Tab enables displaying simultaneously sensors connected to different
channels. It is also possible to overlay a running culture with an existing Culture File.
NOTE: A light blue tab in the run menu indicates that the function is activated, to
deactivate press the tab again. It turns to dark blue.
5.4.3	 Dencytee Sensor Unit
A Dencytee Sensor Unit consists of a Pre-amplifier and a Sensor (Figure 10). They are linked
together via a connecting cable with a VP8 connector. The measurement window has an optical
path length of 5 mm. The seal-less, sapphire window design assures the highest level of sterility,
cleanability and sensor integrity. All wetted parts are USP Class VI certified.
Figure 10: Picture of the assembled Dencytee Unit.
M12 CONNECTOR
CONNECTING CABLE
VP8 CONNECTOR
MEASUREMENT WINDOW
PRE-AMPLIFIERSENSOR
DENCYTEESENSORUNIT
27Cell Density Monitoring System Operating Instructions
PRODUCT DESCRIPTIONPRODUCT DESCRIPTION
Figure 11: Run Menu overview.
CHANNEL TAB SENSOR STATUS LED MULTI-VIEW TAB
MEASUREMENT VALUES
MAIN GRAPHUNIT SETTINGS MENU
MEASURE SETTINGS MENU
FREQUENCY SCAN SETTINGS MENU CULTURE SETTINGS MENU GRAPH ARC MENU
MAIN MENU
29Cell Density Monitoring System Operating Instructions
PRODUCT DESCRIPTIONPRODUCT DESCRIPTION
5.5.2	 Sensor Channel Tab
The Channel Tab (Figure 12) is used to view the measurements of the Sensor Unit connected to
the selected channel. The status LED indicates whether the Sensor Unit is ready for measurement.
The floppy icon indicates whether the measurements are currently recorded in a Culture File.
Recording is indicated by the floppy icon without the red mark (x).
FLOPPY ICON STATUS LED
Figure 12: Sensor Channel Tab.
5.5.3	 Main Menu
The Main Menu (Figure 13) provides access to functions that may not be required on a daily
basis such as calibration, user level settings, export of culture files, or connection to a process
control system.
The Explorer is used to export recorded Culture Files to a USB stick.
The Administrator Menu enables following functionality:
•	 Password control: this functionality allows modifying both the user level and the administrator
level password. (see Chapter 9.1)
•	 General settings: in this menu, it is possible to manage the general settings of the system
such as touch screen calibration and setting of time and date (see Chapter 9.2).
•	 Network applications: this functionality manages network applications such as remote access;
OPC server and file sharing system (see Chapter 9.4).
•	 Manage licenses: this interface allows the installation of new licenses (see Chapter 9.5).
•	 Administrator files: this functionality allows the export of the Controller’s data files (see
Chapter 9.6).
•	 Update system: this enables the update of the Arc View Controller (see Chapter 9.7).
NOTE: Update system functionality is not available for ComBox.
The About Menu contains the hardware and firmware version of the complete measurement
loop including Control Unit and Sensor Units.
The Calibration Menu contains the Sensor Unit calibration and verification (called Validate /
Check Unit Calibration) procedures.
In the Advanced Settings Menu, all adjustments to connect the Control Unit to a process control
system may be defined.
Figure 13: Structure of the Main Menu.
EXPLORER
ADMINISTRATOR MENU
ABOUT MENU
RUN MENU
CALIBRATION MENU
ADVANCED SETTINGS MENU
31Cell Density Monitoring System Operating Instructions
5.5.4	 Unit Settings Menu
The Unit Setting Menu (Figure 14) contains all relevant information of the Sensor Unit connected
to the selected channel, including
•	 Sensor Unit type
•	 Pre-amplifier information, notably the calibration lifetime
•	 Sensor information, e.g. the number of sterilization cycles and the calibration lifetime
Figure 14: Unit Settings Menu.
5.5.5	 Measure Settings Menu
In the Measure Settings Menu (Figure 15) all relevant adjustments for the bioprocess may be set.
In Measure Settings Menu it is possible to
•	 Run a product calibration using the Mark Zero Button
•	 Select the measure mode
•	 Set the Cell Density Unit and Cell Density Factor, which characterize the correlation of the
permittivity measurement with an offline cell density measurement (for Incyte only).
Figure 15: Measure Settings Menu of
Incyte (A) and Dencytee (B).
A
B
PRODUCT DESCRIPTIONPRODUCT DESCRIPTION
33Cell Density Monitoring System Operating Instructions
5.5.6	 Incyte Frequency Scan Settings Menu
The Incyte Frequency Scan may be activated in the Frequency Scan Settings Menu (Figure 16).
The product calibration of the Frequency Scan may also be performed in this Menu using the
Mark Zero Button. It consists of a product calibration of the permittivity measurement at every
frequency used in the Frequency Scan.
Figure 16: Scan display.
5.5.7	 Culture Settings Menu
The Culture Settings Menu (Figure 17) controls all steps required to create a Culture Files:
• Start recording measurements with the Record Button
• Set the inoculation time to log the inoculation time in the Culture File
• Add comments to log manually any event in the Culture File
• End recording to stop logging the measurements in a Culture File
If a recording has been started, the Culture Settings Menu also contains key information relative
to the culture started and the Culture File in which the measurements are logged.
Figure 17: Culture Settings Menu.
PRODUCT DESCRIPTIONPRODUCT DESCRIPTION
35Cell Density Monitoring System Operating Instructions
6	Installation
6.1	 Install the Control Unit
6.1.1	 Install the Arc View Controller
1.	 Inspect the workplace environment for:
	 - Compliance to technical specification
	 - Providing enough space to perform the next steps
2.	 Unpack the Arc View Controller from the packaging
	
	 Figure 18: Open packaging of the Arc View Controller.
3.	 Inspect the Controller and accessories for shipping damage or missing parts:
	 The packaging should contain the Arc View Controller, one test report, the operating
	 instructions, power supply, with 5 country specific cables.
NOTE: The ordered licenses are installed on the Arc View Controller.
4.	 The Arc View Controller may be installed on a bench besides the bioreactor, or mounted on
	 a wall. Please refer to the hardware specifications (Chapter 16.1.1) to choose the appropriate
	 mounting elements to fix the controller.
5.	 Mount the power supply and connect it to the Arc View Controller (Figure 6).
	 The Controller will automatically start up.
6.	 Switch off the Arc View Controller using the Key Switch (Figure 6).
NOTE: It is recommended to perform a Installation Qualification.
NOTE: It is recommended to connect the Arc View Controller to an Uninterruptible
Power Supply (UPS) to ensure continuous recording of data, nevertheless the Controller
restarts after power shutdown automatically at the last setting and continues recording.
After a power shut down the Sensor Unit may require some time for new equilibration.
6.1.2	 Install the ComBox
1.	 Inspect the workplace environment for:
	 - Compliance to technical specification
	 - Providing enough space to perform the next steps
2.	 Unpack the ComBox from the packaging
	
	 Figure 19: Open packaging of the ComBox.
INSTALLATIONINSTALLATION
37Cell Density Monitoring System Operating Instructions
INSTALLATIONINSTALLATION
3.	 Inspect the ComBox and accessories for shipping damage or missing parts: The packaging
	 should contain the ComBox, a power supply, with 5 country specific cables, the operating
	 instructions and a USB stick with the Software and ordered licenses
4.	 The ComBox has to be installed on a bench besides the bioreactor
5.	 Connect the power supply to the ComBox (Figure 7)
6.	 Switch on the PC and, install the Software (see chapter 9.7.1.2) and connect the ComBox by
	 USB, start the Software
7.	 Initialize the M12 connectors (see chapter 9.2.5)
8.	 Re-start the software and install the licenses (see chapter 9.5)
NOTE: It is recommended to perform a Installation Qualification.
6.1.3	 Install the Cell Density Integration Kit
1.	 Inspect the workplace environment for:
	 - Compliance to technical specification
	 - Providing enough space to perform the next steps
2.	 Unpack the ComBox from the packaging
	
	 Figure 20: Open packaging of the Cell Density Integration Kit.
3.	 Inspect the ComBox and accessories for shipping damage or missing parts: The packaging
	 should contain the ComBox, industrial PC (Software and licenses are pre-installed) and the
	 operating instructions
4.	 The Cell Density Integration kit has to be installed inside a skid cabinet
5.	 Connect the industrial PC via the 4-pole connector with the power supply of the cabinet
	 (Figure 8)
6.	 Connect the USB cable of the ComBox to the industrial PC
7.	 The ComBox has to be powered with 24 V via the Modbus Connector (Pin 1 and 3, see
	 chapter 12.1.1)
8.	 Ground the ComBox using the thread on the top
NOTE: It is recommended to perform a Installation Qualification.
6.2	 Mount the Sensor in the Bioreactor
1.	 Unpack your sensor and Pre-amplifier from the protective box
2.	 Inspect the unit for shipping damage or missing parts. The packaging should contain the
	 sensor, the Pre-amplifier and the calibration certificate
	
	 Figure 21: Incyte Sensor Unit.
39Cell Density Monitoring System Operating Instructions
3.	 Select the most appropriate port for mounting the sensor (Figure 22). Choose a position
	 where gas bubbles may not interfere with the measurement, for example in ports opposite
	 to the sparger or at a significant distance. Gas bubbles interfere with the measurement and
	 may cause reading errors. The electrodes of the Incyte sensor have to be kept at least 1 cm
	 away from any solid parts, especially metallic ones. The electric field may be subject to
	 interference and the measurement may be affected.
AGITATION
SPARGER
1 cm1 cm
1cm1cm
A
B
Figure 22: Mounting restrictions, A: Space requirements / B: Reactor mounting restrictions.
4.	Insert the sensor in the port. Do not scratch the platinum electrodes while inserting them.
5.	Verify the sensor orientation to make sure that the Pre-amplifier can be mounted
	 conveniently. The orientation notch of the sensor head is oriented opposite to the
	 Pre-amplifier cable (Figure 23).
NOTE: For mounting from a side-port ensure that the notch of the sensor head is
mounted in a horizontal position facing upwards. Any angle of inclination may lead to an
influence of the measurement.
YES NO
NOTCH OF THE
SENSOR HEAD
NOTCH OF THE
SENSOR HEAD
SIDE PORT
INSTALLATION
BENCH TOP
INSTALLATION
Figure 23: Mounting position of the Pre-amplifier.
NOTE: The vessel should be pressure-less and cold when removing the sensor. The
wear of safety goggles and protective gloves is prescribed.
6.	 Tighten the connection nut by hand.
NOTE: Various housings are available to custom fit your sensor in your installation.
INSTALLATIONINSTALLATION
41Cell Density Monitoring System Operating Instructions
6.3	 Connect the Sensor Unit to the Control Unit
1.	 Switch off the Arc View Controller.
2.	 Verify that the sensor’s male VP connector and the Pre-amplifier’s female VP connector are
dry and clean.
3.	 Align the sensor VP head and the Pre-amplifier connector and insert the Sensor in the
Pre-amplifier.
4.	 Hand-tighten the connector ring of the Pre-amplifier to ensure a robust and waterproof seal
(Figure 25).
5.	 Align the pins of the M12 connector and connect the Arc View Controller to the Pre-amplifier
using the Sensor Unit Cable.
ATTENTION! Make sure that the connector ring is tightened by hand. Never
use a wrench or another tool to tighten or loosen the ring.
ATTENTION! Do not use the Pre-amplifier as a lever to screw or unscrew the
sensor. This may cause mechanical damage in the preamplifier. Always use the
PG13.5 thread instead (Figure 20).
ATTENTION! Please ensure a stable position of the Dencytee Pre-amplifier in
a way that the cable is not bend.
6.	 Switch on the Arc View Controller. Wait at least 30 sec. until the Status LED on the main screen
turns green (Figure 11) and an automated firmware update starts. The status LED of the Incyte
Pre-amplifier (Figure 9) flashes green during this procedure.
NOTE: When receiving a new Sensor Unit, make sure to first connect it to the Arc
View Controller in order to transfer the calibration data. Do not use the Pre-amplifier
with another sensor before the calibration data has been transferred. If you do so the
calibration data will be lost.
1 2 3
Figure 24: Connection of a Sensor Unit.
INSTALLATIONINSTALLATION
43Cell Density Monitoring System Operating Instructions
7	 Operation of Incyte
7.1	 Choose the Measurement Mode
1.	 Go to the Measure Settings Menu (Figure 15). Choose the measurement mode, from the
predefined settings:
• Cell culture > fmes = 1000kHz, fhigh = 10MHz, signal integration = HIGH
• Yeast/fungi > fmes = 2000kHz, fhigh = 10MHz, signal integration = HIGH
• Bacteria > fmes = 1000kHz, fhigh = 10MHz, signal integration = HIGH
2.	 Enter the User password if one has been defined. Press OK.
3.	 Please refer to Chapter 7.12.3 to create your own measurement mode.
7.2	 Addition of Arc Sensors
It is possible to record and plot Arc sensor measurements in parallel to cell density (Figure 25).
That means in addition to the wired Incyte or Dencytee Unit, each Channel can also concurrently
support two Arc Sensors. Cell density is monitored on Chart A. Another measurement
may be shown additionally on Chart A (e.g. Arc pH, Arc DO or the conductivity measurement
of Incyte). The remaining measurements will appear on Chart B. Chart A is shown as the Main
Graph per default.
NOTE: The measurement
values transmitted over
wireless communication are
not intended to be used for
process control.
CHART A
CHART B
EXCHANGE CHART BUTTON
A
B
Figure 25: Run Menu when Arc DO (Chart A) and Arc pH (Chart B) sensors are monitored in parallel to Incyte (A).
The Frequency Scan has been activated (B).
NOTE: The Graph Arc requires Arc Sensors, the Arc Wireless Converter MB
(Ref 243498) or BT (Ref 243499) and Arc Wi Sensor Adapter 1G MB (Ref 242170),
Arc Wi Sensor Adapter 2G MB (Ref 243030), Arc Wi Sensor Adapter 1G BT
(Ref 243460) or Arc Wi Sensor Adapter 2G BT (Ref 243470).
1.	 Mount the Arc Wi Sensor Adapter between the Arc sensor and the Arc Cable VP 8 (Figure 26).
Figure 26: Mounting an Arc Sensor.
OPERATION OF INCYTEOPERATION OF INCYTE
ARC SENSOR
ARC WI SENSOR ADAPTER FOR
WIRELESS FUNCTIONALITY
VP8 CONNECTOR
45Cell Density Monitoring System Operating Instructions
2.	 Plug the Arc Wireless Converter MB or BT in one of the USB Ports on the side panel of the
Arc View Controller.
3.	 Press the button Graph Arc on the bottom left hand corner of the Run Menu (Figure 11). The
Arc View Controller will automatically scan for sensors. The Arc Wireless Converter MB or
BT is flashing in red during this process. The Graph Arc display opens (Figure 27). The cell
density measurement is set on Chart A per default.
4.	 Choose the second parameter to be shown on Chart A and press OK. The remaining
parameters may be shown on Chart B.
Figure 27: Graph Arc Menu.
5.	 Chart A is displayed per default on the main graph (Figure 23). Chart B is shown in the lower
left corner. Press the Exchange Chart Button (Figure 23) to switch the display of the two
charts. Chart B is not visible if the Incyte Scan mode is activated. It may be exchanged with
Chart A on the Main Graph using the Exchange Chart Button.
7.3	 Start Recording Measurements in a Culture File
1.	 Verify that the Status Indicator on the Run Menu of the Arc View Controller (Figure 11) and
the status LED of the Pre-amplifier are green (Figure 9).
2.	 Go to the Culture Settings Menu and press the Record Button (Figure 17).
3.	 Enter the User password if one has been defined. Press OK.
4.	 Follow the guided procedure to start recording the Culture file. Define the directory to save
the file, per default it is the home directory. Select the acquisition time, enter the optional
name of the Culture file. Add possible comments and the user name.
5.	 The Record Button is now disabled, and the information relative to the Culture and the Culture
File appears in the Culture Settings Menu. The Floppy Icon on the Channel Tab (Figure 12)
also confirms that the measurements are being recorded.
6.	 Start the Frequency scan if required (Chapter 7.12.2).
7.4	 Perform a Product Calibration and Set the Inoculation Time
NOTE: Even though the Dual Frequency Measurement Mode reduces the influence
of medium and medium changes on the measurement, it is usual to do a product
calibration, i.e. a zero-adjustment before inoculation. The product calibration may be
done automatically, which is the preferred setting. If the background signal is known the
product calibration may be done manually or adjusted over time (see Chapter 7.12.4).
1.	 Equilibrate the Incyte Sensor in culture medium, for at least 30 minutes prior to product
calibration and inoculation.
2.	 Verify on the Main Graph that the cell density measurement is stable.
3.	 Go to the Measure Settings Menu and press the Mark Zero Button to perform a product
calibration (Figure 15).
4.	 Enter the User password if one has been defined. Press OK.
5.	 Select automatic. The cell density measurement is now compensated for an offset shown in
the measurement values above the Main Graph on the Run Menu.
6.	 To assign the inoculation time, go to the Culture Settings Menu and press the Inoculate
Button (Figure 17). The time on the Main Graph will be reset to zero. A new time scale is
added to the Culture File to track the real culture time.
7.	 Enter the User password if one has been defined. Press OK.
7.5	 Add a Comment to the Culture File
During recording, a comment may be added at any time to the Culture File. This functionality
may be used to track offline samplings.
1.	 Go to the Culture Settings Menu and press the Comments Button (Figure 17).
OPERATION OF INCYTEOPERATION OF INCYTE
47Cell Density Monitoring System Operating Instructions
2.	 Enter the User password if one has been defined. Press OK.
3.	 Enter the title and the description of the comment. Both information are recorded in the
Culture File but only the title is shown on the Main Graph.
7.6	 Customize the Main Graph
In the Run Menu it is possible to adjust the visualization settings of the Main Graph.
•	 Press the Auto Scale Button to set an automated scaling.
•	 Press the Follow Button, to track the current cell density measurements.
•	 To display a certain part of the graph, move the graph to the left or the right using the
Move Right and Move Left Buttons.
•	 Click on the Zoom In Button to enlarge a detail in the graph and use the Zoom Out Button
to set back.
•	 Click on the X Scale or Y Scale Buttons to scale a specific area of the Main Graph
between Xmin and Xmax respectively Ymin and Ymax. Both Y axes may be scaled
independently by selecting axis 2.
•	 Clear the graph at the end of the Culture by pressing the Clear Button.
Figure 28: Visualization Settings of the Main Graph.
7.7	 Setup a Multi View Graph
In the Multi-View Tab (Figure 29), it is possible to plot the measurements of several channels on
the same graph. In addition the current measurements may be plotted in parallel to a previously
recorded Culture File.
NOTE: In the Multi-View Graph you can display two of five measurements at the
same time.
1.	 Go to the Multi-View Tab and select Graph 1.
2.	 Tick the Display Graph Check Box and enter a Title.
3.	 Select the Channel(s) to be displayed.
4.	 Select a recorded Culture File to be viewed.
5.	 Select the Measurements to be compared, e.g. cell density, Incyte Scan or conductivity (σ).
If using the Incyte scan the following values are also available fc, Δε, and α.
6.	 After definition of the Multi-View, the chosen Measurements are plotted on the Multi-View
Graph. All current measurement values are displayed on the upper right area.
Figure 29: Multi View setup.
Y-SCALE BUTTON
CLEAR BUTTON
X-SCALE BUTTON
AUTO SCALE BUTTON FOLLOW BUTTON
MOVE LEFT BUTTON MOVE RIGHT BUTTON
ZOOM IN BUTTON ZOOM OUT BUTTON
OPERATION OF INCYTEOPERATION OF INCYTE
49Cell Density Monitoring System Operating Instructions
7.8	 Stop Recording the Culture File
1.	 Go to the Culture Settings Menu and press the End Recording Button (Figure 17).
2.	 Enter the User password if one has been defined. Press OK.
3.	 Export the culture file (Chapter 7.10).
4.	 Dismount the Sensor (Chapter 7.9).
7.9	 Dismount the Sensor Unit
1.	 Switch off the Arc View Controller using the Key Switch.
2.	 Disconnect the Sensor Unit Cable from the Pre-amplifier.
3.	 Loosen the connector ring of the Pre-amplifier by hand and remove the Pre-Amplifier from
the Sensor (Figure 24).
4.	 Loosen the thread of the sensor manually and remove the sensor from the bioreactor.
ATTENTION: Make sure that the connector ring is loosen by hand. Never use a
wrench or another tool to tighten or loosened the ring.
ATTENTION: Do not use the Pre-amplifier as a lever to screw or unscrew the
sensor. This may cause mechanical damage in the preamplifier. Always use the
PG13.5 thread instead (Figure 24).
7.10	 Export a Culture File
1.	 Go to the Main Menu and press the Explorer Button (Figure 13). The Explorer (Figure 30)
displays simultaneously two directories; the Arc View Controller Home per default. Select
USB for the second directory.
2.	 Enter the User password if one has been defined. Press OK.
3.	 Plug a USB Stick on the side panel of the Arc View Controller (Figure 7) or PC, if using the
ComBox (Figure 8). Click on the refresh, select the USB stick and press open dir.
4.	 Select the Culture File in the home directory and press the Copy Button.
5.	 Refresh the USB directory, and verify that the Culture File has been transferred successfully.
6.	 Delete the Culture File copied to free storage space.
7.	 If needed, the Culture File may be renamed, by selecting the file and pressing the Rename
Button.
8.	 It is possible to create a new directory by pressing the Create Directory Button.
NOTE: Screenshots (see Chapter 11.4.1) are also stored in the Arc View Controller
Home directory.
Figure 30: Explorer Menu.
OPERATION OF INCYTEOPERATION OF INCYTE
51Cell Density Monitoring System Operating Instructions
7.11	 Import a Culture File in an Excel Spreadsheet
1.	 Open Microsoft Excel and select the Data Tab.
2.	 In the area Get External Data press the From Text Icon.
3.	 Choose the Culture file and click open.
4.	 Mark the column Date and use a User-defined Format to display the time recordings: yyyy.
mm.dd.hh.mm.ss.
5.	 Select the columns Record and Culture time and define the format as Numbers.
The file (Figure 31) is composed of three parts. The Header contains general information on the
Culture. The Events list contains all significant events and modifications that happened while the
culture file was recorded. The Measurement table with the following measurement values:
•	Date
•	 Record time (h): time in hours since the start of recording
•	 Culture time (h): time in hours since the inoculation
•	 Comment: contains start recording, inoculation, comments and stop recording
•	 Raw permittivity measurements
•	 Cell density value correlated to the product calibration and cell factor
HEADER
EVENTS
MEASUREMENT
Figure 31: Culture File.
7.12	 Further Measurement Settings
7.12.1	 Set the Cell Factor
The measured permittivity may be correlated to offline measurements, by defining a cell factor.
1.	 Go to the Measure Tab (Figure 15) and press the Cell Density Unit / Factor Button.
2.	 Enter the User password if one has been defined. Press OK.
3.	 Choose a UnitFactor from the drop-down list and press edit. Insert the Cell Factor. To learn
how to calculate the cell factor refer to Chapter 18.2.
4.	 The Cell Factor is displayed as measurement value on top of the Main Graph (Figure 11). Per
default the cell factor is 1.
NOTE: The Permittivity Factor 1.00 pF/cm is the factory value, which cannot be
changed. This is the factor the measurement is relying on. The UnitFactors are the
correlated customer factors.
7.12.2	 Start the Incyte Scan
NOTE: The Scan License is required to use the Incyte Scan option.
1.	 Go to the Scan Settings Menu (Figure 6) and activate the Frequency Scan.
2.	 Enter the User password if one has been defined. Press OK.
3.	 The curve of the Frequency Scan is displayed in the Run Menu, below the Main Graph.
Δεmax, fc and α are calculated and shown as well (Figure 32).
4.	 Equilibrate the sensor in the culture medium for at least 30 minutes.
5.	 Go to the Scan Settings Menu (Figure 6) and press the Mark Zero Button to perform the
product calibration of the Frequency Scan.
OPERATION OF INCYTEOPERATION OF INCYTE
53Cell Density Monitoring System Operating Instructions
DISPLAY OF INCYTE
FREQUENCY SCAN
Figure 32: Run Menu with activated Incyte Frequency Scan.
7.12.3	 Define the Custom Measurement Mode
The measurement frequencies, integration time and pulse filter may be adjusted to define a
custom Measurement Mode.
1.	 Go to the Measure Tab (Figure 15) and select MeasureMode1 in the drop down menu.
Press the Edit Button.
2.	 Enter the User password if one has been defined. Press OK.
3.	 Enter a new name for the measure mode. Define the measurement frequency (fmes) and the
background frequency (fhigh) (Figure 33).
4.	 Select the integration time, which creates a moving average over a defined number of
measurements:
•	 NONE: 1 measurement - Integration time : 6 sec
•	 LOW: 3 measurement3 - Integration time : 18 sec
•	 MEDIUM: 22 measurements - Integration time : 2.2 min
•	 HIGH: 111 measurements : Integration time : 11.1 min (default)
•	 SUPER HIGH: 333 measurements - Integration time : 33.3 min
•	 Custom, whereas 6 sec is the minimum time
5.	 If required activate the Pulse filter. The Pulse filter is only necessary for media with a high
content of solid particles.
6.	 Press the Save Button.
Figure 33: Custom Measurement Mode.
7.12.4	 Perform a Manual Product Calibration
1.	 Go to the Measure Settings Menu and press the Mark Zero Button to perform a product
calibration (Figure 15).
2.	 Enter the User password if one has been defined. Press OK.
3.	 Select manual and compensate the cell density measurement manually. Enter the known
permittivity of the background. The cell density measurement is now compensated for an
offset shown in the measurement values above the Main Graph on the Run Menu.
7.13	 Use Sensor Cleaning (Probe Cleaning)
The Sensor Cleaning is deactivated per default. It is only required if cell adhesion at the sensor
tip is noticed. The Cleaning function reduces the attachment of cells and may be required in few
processes, i.e. in long-term cell culture or fermentation of filamentous fungi. Use the cleaning
mode with caution and only if adhesion of cells at the platinum electrodes is noticed or suspected.
Start with SHORT cleaning time and a long Auto-Cleaning Period, at least 12 h. Increase
cleaning time / decrease the Auto-Cleaning Period only if no improvement is observed.
1.	 Enter the Advanced Settings Menu and select Probe cleaning.
2.	 Enter the User password if one has been defined. Press OK.
OPERATION OF INCYTEOPERATION OF INCYTE
55Cell Density Monitoring System Operating Instructions
Figure 34: Sensor Cleaning.
3.	 Choose between the Manual or Auto Cleaning. The auto cleaning consists of periodic
cleaning cycles, which start automatically after defined period whereas in the manual
cleaning mode the user starts a cleaning cycle manually.
4.	 Go to the Run Menu and press the Measure Button.
5.	 Select the duration of the Cleaning: short (cleaning time 30 s) or long (cleaning time 2 min).
6.	 Enter the User password if one has been defined. Press OK.
7.	 Activate the Auto-Cleaning (Figure 35) and define the Auto-Cleaning Period, i.e. the time
between two Auto-Cleaning cycles.
8.	 Alternatively, press Clean now to start a Cleaning cycle manually directly.
9.	 Confirm the password request, without entering any code (see Chapter 9.1).
NOTE: Measurements are unavailable during a cleaning cycle. The status LED on the
Pre-amplifier (Figure 9) is flashing green during this procedure. The sensor may need
time for equilibration after the cleaning cycle.
Figure 35: Selection of the cleaning mode.
8	 Operation of Dencytee
8.1	 Choose the Measurement Mode
1.	 Go to the Measure Settings Menu (Figure 15). Choose the measurement mode, from the
predefined settings of Measure Mode 1, 2 or 3, which are a combination of pulse filter and
signal integration.
2.	 Enter the User password if one has been defined. Press OK.
3.	 To create an own measurement mode see Chapter 8.12
8.2	 Addition of Arc Sensors
It is possible to record and plot Arc sensor measurements, in parallel to cell density (Figure 36).
That means in addition to the wired Incyte or Dencytee Unit, each channel can also concurrently
support two Arc Sensors. Cell density is monitored on Chart A. Another measurement may be
shown additionally on Chart A (e.g. Arc pH, Arc DO). The remaining measurements will appear
on Chart B. Chart A is shown as the Main Graph per default.
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57Cell Density Monitoring System Operating Instructions
NOTE: The measurement values transmitted over wireless communication are not
intended to be used for process control.
CHART A
CHART B
EXCHANGE
CHART BUTTON
Figure 36: Cell Density Graph with Arc DO, and Arc pH records.
NOTE: The Graph Arc requires Arc Sensors, the Arc Wireless Converter MB
(Ref 243498) or BT (Ref 243499) and Arc Wi Sensor Adapter 1G MB (Ref 242170),
Arc Wi Sensor Adapter 2G MB (Ref 243030), Arc Wi Sensor Adapter 1G BT
(Ref 243460) or Arc Wi Sensor Adapter 2G BT (Ref 243470).
OPERATION OF DENCYTEEOPERATION OF DENCYTEE
1.	 Mount the Arc Wi Sensor Adapter between the Arc sensor and the Arc Cable VP 8 (Figure 37).
ARC SENSOR
ARC WI SENSOR ADAPTER FOR
WIRELESS FUNCTIONALITY
VP8 CONNECTOR
Figure 37: Mounting an Arc Sensor.
2.	 Plug the Arc Wireless Converter MB or BT in one of the USB Ports on the side panel of the
Arc View Controller.
3.	 Press the button Graph Arc on the bottom left hand corner of the Run Menu (Figure 11). The
Arc View Controller will automatically scan for sensors. The Arc Wireless Converter MB or
BT is flashing in red during this process. The Graph Arc display opens (Figure 38). The cell
density measurement Dencytee is set on Chart A per default.
4.	 Choose the second parameter to be shown on Chart A and press OK. The remaining
parameters will be shown on Chart B.
59Cell Density Monitoring System Operating Instructions
Figure 38: Graph Arc Menu.
5.	 Chart A is displayed per default on the main graph (Figure 36). Chart B is shown in the
lower left corner. Press the Exchange Chart Button (Figure 36) to switch the display of the
both charts.
8.3	 Start Recording Measurements in a Culture File
1.	 Verify that the Status Indicator on the Run Menu of the Arc View Controller (Figure 11) is green.
2.	 Go to the Culture Settings Menu and press the Record Button (Figure 17).
3.	 Enter the User password if one has been defined. Press OK.
4.	 Follow the guided procedure to start recording the Culture file. Define the directory to save
the file, per default it is the home directory. Select the acquisition time, enter the optional
name of the Culture file. Add possible comments and the user name.
5.	 The Record Button is now disabled, and the information relative to the Culture and the
Culture File appears in the Culture Settings Menu. The Floppy Icon on the Channel Tab
(Figure 12) also confirms that the measurements are being recorded.
8.4	 Perform Product Calibration and Set the Inoculation Time
NOTE: Even though our measurement principle is not influenced by the media color,
it is usual to do a product calibration, i.e. a zero-adjustment before inoculation.
1.	 Equilibrate the Incyte Sensor in culture medium, for at least 30 minutes prior to product calibration
and inoculation.
2.	 Verify on the Main Graph that the cell density measurement is stable.
3.	 Go to the Measure Settings Menu and press the Mark Zero Button to perform a product
calibration (Figure 15).
4.	 Enter the User password if one has been defined. Press OK.
5.	 Select automatic. The cell density measurement is now compensated for an offset shown in
the measurement values above the Main Graph on the Run Menu.
6.	 To assign the inoculation time, go to the Culture Settings Menu and press the Inoculate
Button (Figure 17). The time on the Main Graph will be reset to zero. A new time scale is
added to the Culture File to track the real culture time.
8.5	 Add a Comment to the Culture File
During recording, a comment may be added any time to the Culture File. This functionality may
be used to track offline samplings.
1.	 Go to the Culture Settings Menu and press the Comments Button (Figure 17).
2.	 Enter the User password if one has been defined. Press OK.
3.	 Enter the title and the description of the comment. Both information are recorded in the
Culture File but only the title is shown on the Main Graph.
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61Cell Density Monitoring System Operating Instructions
8.6	 Customize the Main Graph
In the Run Menu it is possible to adjust the visualization settings of the Main Graph.
•	 Press the Auto Scale Button to set an automated scaling.
•	 Press the Follow Button, to track the current cell density measurements.
•	 To display a certain part of the graph, move the graph to the left or the right using the
Move Right and Move Left Buttons.
•	 Click on the Zoom In Button to enlarge a detail in the graph and use the Zoom Out Button
to set back.
•	 Click on the X Scale or Y Scale Buttons to scale a specific area of the Main Graph
between Xmin and Xmax respectively Ymin and Ymax. Both Y axes may be scaled
independently by selecting axis 2.
•	 Clear the graph at the end of the Culture by pressing the Clear Button.
Figure 39: Adjustments on the Main Graph.
8.7	 Setup a Multi View Graph
In the Multi-View Tab (Figure 40), it is possible to plot Incyte and Dencytee measurements in the
same graph. In addition the current data may be plotted in parallel to the graph of a previous
recorded culture file
NOTE: When setting up of the Multi-View, be aware that only 2 types of curves can
be displayed on a single graph.
1.	 Go to the Multi-View Tab and select Graph 1 (Figure 40), enter a title to label the Multi-View
graph.
2.	 Tick the display graph button and select the channel to be displayed.
3.	 Select a recorded culture file in the File selection.
4.	 Define the measurement type, e.g. cell density.
5.	 After definition of the Multi-View, all measurements are plotted in the defined Multi-View
Graph.
Figure 40: Multi View setup.
Y-SCALE BUTTON
CLEAR BUTTON
X-SCALE BUTTON
AUTO SCALE BUTTON FOLLOW BUTTON
MOVE LEFT BUTTON MOVE RIGHT BUTTON
ZOOM IN BUTTON ZOOM OUT BUTTON
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63Cell Density Monitoring System Operating Instructions
8.8	 Stop Recording the Culture File
1.	 Go to the Culture Settings Menu and press the End Recording Button (Figure 17).
2.	 Enter the User password if one has been defined. Press OK.
3.	 Export the culture file (Chapter 8.10).
4.	 Dismount the Sensor (Chapter 8.9).
8.9	 Dismount the Sensor Unit
1.	 Switch off the Arc View Controller using the Key Switch.
2.	 Disconnect the Sensor Unit Cable from the Pre-amplifier.
3.	 Loosen the connector ring of the Pre-amplifier by hand and remove the Pre-Amplifier from
the Sensor (Figure 24).
4.	 Loosen the thread of the sensor manually and remove the sensor from the bioreactor.
ATTENTION! Make sure that the connector ring is loosen by hand. Never use a
wrench or another tool to tighten or loosen the ring (Figure 24).
8.10	 Export a Culture File
1.	 Go to the Main Menu and press the Explorer Button (Figure 13). The Explorer (Figure 41)
displays simultaneously two directories; the Arc View Controller Home per default. Select
USB for the second directory.
2.	 Enter the User password if one has been defined. Press OK.
3.	 Plug a USB Stick on the side panel of the Arc View Controller (Figure 7) or PC, if using the
ComBox (Figure 8). Click on the refresh, select the USB stick and press open dir.
4.	 Select the Culture file in the home directory and press the Copy Button.
5.	 Refresh the USB directory, and verify that the Culture file has been transferred successfully.
6.	 Delete the Culture file copied to free storage space.
7.	 If needed, the Culture file may be renamed, by selecting the file and pressing the Rename
Button.
8.	 It is possible to create a new directory by pressing the Create Directory Button.
NOTE: Screenshots (see Chapter 11.4.1) are also stored in the Arc View Controller
Home directory.
Figure 41: Explorer Menu.
8.11	 Import the Culture File in an Excel Spreadsheet
1.	 Open Microsoft Excel and select the Data Tab.
2.	 In the area Get External Data press the From Text Icon.
3.	 Choose the Culture file and click open.
4.	 Mark the column Date and use a User-defined Format to display the time recordings: yyyy.
mm.dd.hh.mm.ss.
5.	 Select the columns Record and Culture time and define the format as Numbers.
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65Cell Density Monitoring System Operating Instructions
The file (Figure 42) is composed of three parts. The Header contains general information on the
Culture. The Events list contains all significant events and modifications that happened while the
culture file was recorded. The Measurement table with the following measurement values:
•	Date
•	 Record time (h): time in hours since the start of Recording
•	 Culture time (h): time in hours since the inoculation
•	 Comment: contains start recording, inoculation, comments and stop recording
•	 Raw turbidity measurements
•	 Cell density value correlated to the product calibration and cell factor a
HEADER
EVENTS
MEASUREMENT
Figure 42: Culture File
8.12	 Define the Custom Measurement Mode
The measurement, integration time and pulse filter may be adjusted to define the custom
Measurement Mode.
1.	 Go to the Measure Tab (Figure 15) and select MeasureMode1 in the drop down menu.
Press the Edit Button.
2.	 Enter the User password if one has been defined. Press OK.
3.	 Enter a new name for the measure mode (Figure 43).
4.	 Select the integration time, this creates a moving average over a defined number of
measurements
•	 NONE: 1 measurement - Integration time : 6 sec
•	 LOW: 3 measurement3 - Integration time : 18 sec
•	 MEDIUM: 22 measurements - Integration time : 2.2 min
•	 HIGH: 111 measurements : Integration time : 11.1 min (default)
•	 SUPER HIGH: 333 measurements - Integration time : 33.3 min
•	 Custom, whereas 6 sec is the minimum time
5.	 Activate the Pulse filter. The pulse filter is only necessary for media with a high content of
solid particles.
6.	 Press the Save Button.
Figure 43: Create new measure mode.
9	 Administrator Menu
From the Administrator Menu all system settings are accessible, including password control,
network settings and applications, general settings, like the touch screen calibration (only Arc
View Controller), the possibility to install licenses or update the system and transfer the Administrator
Files.
NOTE: To enter the Administrator Menu, a password is requested, enter the code
14147125 and press ok.
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67Cell Density Monitoring System Operating Instructions
9.1	 Definition of the User Level
To limit the access to the Arc View Controller, following User Level may be defined (Figure 44):
•	 Viewer: permission to see the Run Menu, no password needed
•	 User: permission to change the Run Settings, a User password may be defined
•	 Administrator: complete access to the Arc View Controller, default code 14147125
NOTE: As soon as a User Level password is defined, there is no possibility to delete it.
Figure 44: Definition of the User Level.
9.1.1	 Change the Administrator Password
1.	 Enter the Administrator Menu.
2.	 Press the Password Control button.
3.	 Insert the existing password in the old password-box of the Administrator level and define a
new one, confirm it and press validate.
NOTE: Please note the new administrator password.
ADMINISTRATOR MENUADMINISTRATOR MENU
9.1.2	 Define the User Level
1.	 Enter the Administrator Menu.
2.	 Press the Password Control button.
3.	 Leave the old password-box of the User level empty and define a new one, confirm it and
press validate.
NOTE: Please note the new user password.
9.2	 Adjust the General Settings
In the General Settings (Figure 45) it is possible to perform a touch screen calibration, adjust the
decimal separator in the Culture file, activate the continuous plotting of the cell density measurement
(without recording), adjust the time and date settings. Additionally it is possible to initialize
the M12 connectors and activate the Cell Density Integration Kit option.
Figure 45: Adjustment of the general settings.
69Cell Density Monitoring System Operating Instructions
9.2.1	 Touch Screen Calibration
1.	 Enter the Administrator Menu and press general settings button.
2.	 Select touch screen calibration and follow the instructions on the Main Display.
NOTE: Available for the Arc View Controller only.
9.2.2	 Adjustment of the Decimal Separator in the Culture File
If the decimal separate output is not compatible to the output software, e.g. Windows Excel, the
record file may be adjusted.
1.	 Enter the Administrator Menu and press general settings button.
2.	 Select the decimal separator to be dot or comma, per default international is selected.
9.2.3	 Continuous Display of Graphed Data
Always display the graph may be activated, to plot the current measurements without recording.
1.	 Enter the Administrator Menu and press general settings button.
2.	 Activate the Chart options and the graph is plotted continuously.
9.2.4	 Adjustment of Date and Time Settings
NOTE: Available only for the Arc View Controller and Cell Density Integration Kit (if
screen connected).
Time and date may be adjusted if displayed incorrect.
1.	 Enter the Administrator Menu and press general settings button.
2.	 Press set time and adjust date, time and time-zone (offset to UTC).
3.	 Press OK, the Control Unit requests for a re-start to change the date and time settings.
9.2.5	 Initialize the Arc View M12 Connectors
NOTE: You can use 1 of the combinations below for Channel assignment (including
Aux and Modbus channels):
1. M12 cable plus Pre-amplifier with sensor attached
2. M12 cable plus Pre-amplifier with Cal Simulator attached
3. M12 cable plus Pre-amplifier only
Initialize the M12 connectors after a Software update (see Chapter 9.7.1), or the re-set of the
factory settings (see Chapter 9.7.2), This ensures that the Sensor Units are correctly detected.
1.	 Enter the Administrator Menu and press general settings button.
2.	 Press Initialization of the Control Unit and follow the instructions on the main display.
9.2.6	 Enable the Cell Density Integration Kit Option
NOTE: This procedure enables the verification and calibration by Modbus, but
disables this possibility on the screen. It is only required if the procedure should run via
Modbus, e.g. if the system is integrated in a process control system.
1.	 Enter the Administrator Menu and press the general settings button.
2.	 Activate the Cell Density Integration Kit option
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71Cell Density Monitoring System Operating Instructions
ADMINISTRATOR MENUADMINISTRATOR MENU
9.3	 Read the Network Settings
NOTE: Available only for the Arc View Controller and Cell Density Integration Kit (if
screen connected).
In the Network Settings it is possible to read the Serial Number (Device Name) of the Arc
View Controller and Cell Density Integration Kit, as well as the max address, IP address, subnet
mask and gateway address (Figure 42). This information is relevant for the connection of
the Arc View Controller and Cell Density Integration Kit to the process control system via OPC
or the integration of the Controller or Kit in a network.
Figure 46: Network Settings display.
The Network Settings page lets you configure the network configuration of your device.
In order to use the networking features of the device, an IP address must be assigned.
Depending on the configuration of the network where you intend to install the device, one
of the following cases applies:
•	 Manual IP addressing (checkbox not selected)
•	 Automatic IP addressing (checkbox selected)
Manual IP addressing means that the IP configuration is given by the network administrator. The
relevant information (IP address of your device, subnet mask, gateway) must be entered in the
corresponding fields. Depending on your company's policy, you should not take an unassigned
IP address randomly for your device since it may result in networking problems.
Dynamic addressing means that the IP configuration is given by a service, called DHCP, running
on the network. This is the default configuration and is suitable in most cases.
NOTE: Check, when troubleshooting dynamic IP addressing:
•	Cabling
•	 DHCP service is running on the physical network where your Control Unit is connected.
•	 Check with your system administrator if the policy requires a manual entry of the
	 device's MAC address in the DHCP configuration. The MAC address is written on the
	 small sticker with barcode located on the main board. In addition the MAC address is
	 shown in the Network Settings View.
9.4	 Adjust the Network Applications
In the Network Applications it is possible to activate share data files for the integration in a Windows
network. Additionally the OPC communication (see Chapter 12.2) may be enabled (Figure 47).
Figure 47: Activate the Network Applications.
73Cell Density Monitoring System Operating Instructions
9.4.1	 Activate Share Data Files
NOTE: Available only for the Arc View Controller and Cell Density Integration Kit (if
screen connected).
1.	 Enter the Administrator Menu and press the Network Applications button.
2.	 Activate the share data files to enable the integration of the Control Unit into a Windows
Network.
3.	 Set Up the integration in the local network, using the Network Settings (see Chapter 9.3).
9.4.2	 Activate OPC Communication
1.	 Enter the Administrator Menu and press the Network Applications button.
2.	 Activate OPC to allow OPC communication to the process control system (see Chapter 12.2).
9.5	 Install a License
NOTE: The ordered licenses are installed on the Arc View Controller.
The ordered licenses are stored on the USB stick provided with the ComBox.
1.	 Enter the Administrator Menu and press manage license.
2.	 Connect the USB stick with the new license on one of the USB ports at the right panel
(Figure 6) of the Arc View Controller, or a PC where the ComBox is connected.
3.	 Click the load license button and select the license and press ok (Figure 48).
NOTE: After installation of the OPC license a re-start is requested.
Figure 48: License Menu.
9.6	 Transfer Administrator Files
The Administrator files (Figure 49) may be transferred for storage in an external hard drive or
used for trouble shooting purposes.
Figure 49: Transfer the administrator files.
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75Cell Density Monitoring System Operating Instructions
9.6.1	 Export the Log Files
NOTE: Available only for the Arc View Controller and Cell Density Integration Kit (if
screen connected).
1.	 Enter the Administrator Menu and press Administrator Files.
2.	 Connect a USB stick on one of the USB ports at the right panel (Figure 6) of the Arc View
Controller, or the industrial PC of the Cell Density Integration Kit.
3.	 Follow the instructions on the Main Display. The log files may be needed for trouble shooting
the system.
9.6.2	 Erase Temporary Files
NOTE: Available only for the Arc View Controller and Cell Density Integration Kit (if
screen connected).
1.	 Enter the Administrator Menu and press Administrator Files.
2.	 Press Erase temporary files to clean the memory of the Arc View Controller, or the industrial
PC of the Cell Density Integration Kit.
3.	 Follow the instruction on the main display of the Control Unit.
9.6.3	 Export Culture Data
NOTE: This function is available starting with Software Version 3.8.
1.	 Enter the Administrator Menu and press Administrator Files.
2.	 Select the Channel, to export the corresponding data, and enter the initial and final date for
the export.
3.	 Connect a USB stick on one of the USB ports at the right panel (Figure 6) of the Arc View
Controller or the PC, where the ComBox is Installed and follow the instruction on the Main
Display of the Control Unit.
9.7	 Update or Re-Set the Arc View Controller or
	 Cell Density Integration Kit
In the Update System Menu (Figure 50), it is possible to re-set the Control Unit to the factory
Software, or update the system.
Figure 50: Update System Setting.
NOTE: The settings are reset to factory when updating the Control Unit.
9.7.1	 Software Update of the Control Unit
9.7.1.1	 Software Update of the Arc View Controller or
	 Cell Density Integration Kit
1.	 Please check our Homepage www.hamiltoncompany.com/arc_view_update for new updates.
Save the file on an USB stick.
2.	 Enter the Administrator Menu and press Update system.
3.	 Connect the USB stick to the Arc View Controller and select the update file. Activate the
system update.
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77Cell Density Monitoring System Operating Instructions
4.	 Follow the instructions of the Main Display.
5.	 After the update is finished initialize the M12 Connectors (see Chapter 9.2.5).
9.7.1.2	 Software installation/update of the ComBox
NOTE: Prior to the update, save the current data.
9.7.1.2.1	 Procedure for one ComBox per PC
1.	 Please check our Homepage www.hamiltoncompany.com/arc_view_update for the newest
Software version.
2.	Download.
3.	 Execute the "Combox_Setup.exe".
4.	 Follow the instructions on the screen, until the Software is installed.
5.	 Connect the ComBox and close the Installer Software.
6.	 Start the Software and initialize the M12 connectors (see Chapter 9.2.5), close the Software.
7.	 Start the Software and install the licenses (see Chapter 9.5).
9.7.1.2.2	 Procedure for two ComBoxes per PC
1.	 Follow steps 1 and 5 in Chapter 9.7.1.2.1.
2.	 Duplicate folder "C:\Program Files (x86)\Hamilton\CellDensityComBox".
3.	 Adjust the folder name, while adding the number of the box, e.g. "...ComBox_2".
4.	 Create a shortcut for the "interface_Biomas_rack_V2.exe" in the new folder.
5.	 Save shortcut on the desktop under the defined name (in this case ...2).
7.	 Connect ComBox 2 and start Software 2.
8.	 Switch to the first Software, initialize the Channels (see Chapter 9.2.5) and install the licenses
(see Chapter 9.5).
9.	 Switch to the second Software, initialize the Channels (see Chapter 9.2.5) and install the
licenses (see Chapter 9.5).
ADMINISTRATOR MENUADMINISTRATOR MENU
NOTE: If you have more than two ComBoxes, repeat the procedure for the amount of
ComBoxes available.
9.7.2	 Re-Set the Arc View Controller or Cell Density Integration Kit
In case of Software failure, it is possible to re-set the factory settings.
1.	 Enter the Administrator Menu and press Update system.
2.	 Connect a USB Keyboard and a USB Mouse to the USB connections of the Arc View
Controller (Figure 6), or industrial PC of the Cell Density Integration Kit (Figure 8).
3.	 Press factory version, to re-set the factory setting of the Control Unit.
4.	 Follow the instructions on the Main Display.
5.	 After the system is re-set initialize the M12 Connectors (see Chapter 9.2.5).
9.7.3	 Update Recovery of the Arc View Controller
In case of script update, it is possible to perform a recovery update process instead of a complete
software update.
NOTE: This functionality is only available for Arc View Controller.
1.	 Connect the USB stick with the scripts (provided by Hamilton) to the Arc View Controller.
2.	 Enter the Administrator Menu select the .zip file, which contains the recovery scripts and
press Update recovery. The software now checks for completeness of the data and copies
the files to the recovery partition.
3.	 Upon completion the Arc View Controller requires two re-starts, which which have to be
confirmed after pressing Update recovery.
NOTE: If a wrong file is selected a instruction for the correct procedure will be
displayed on the Controller screen.
79Cell Density Monitoring System Operating Instructions
10	Maintenance
10.1	 Daily Maintenance
As a daily routine, at least prior to every run, the Status Indication on the Arc View Controller
may be checked (Figure 12). The Indicator should be green, if not please refer to Chapter 11.1.
10.2	 Weekly Maintenance
The Weekly Maintenance should be performed on a weekly basis or at the end of a Culture.
1.	 Check the Status Indicator on the Arc View Controller (Figure 12). The Indicator should be
green.
2.	 Decontaminate the sensor by Sterilization in Place, Cleaning in Place or Autoclavation.
3.	 Press the increment sterilization button (Figure 14) in the Unit Tab after autoclavation.
4.	 If needed perform the Manual Cleaning Procedure (see Chapter 10.3).
5.	 Download the recent Culture Files if not yet done.
NOTE: Incyte and Dencytee Sensors need to be replaced at least after 100 Autoclavation,
Sterilization-in place, or Cleaning-in-place cycles. The Status Indicator on the Arc
View Controller turns orange and a request to replace the sensor appears. Failure to do
so may lead to measurement errors.
ATTENTION! The pre-Amplifier is not autoclavable. Dismount it prior to
autoclavation to avoid damages to the electronics Please refer to Chapter 7.9
and 8.9 for instructions. Protect the sensor’s male connector with aluminum
foil to prevent condensed vapors from reaching the electrical contacts during
sterilization.
10.3	 Manual Cleaning Procedure at Process End
	 for Cell Density Sensors
Manual cleaning may be needed, when a thick biofilm sticks on the sensor.
10.3.1	 Incyte Sensors
1.	 After decontamination, immerse the PEEK sensor tip in Sodium Sulfite (Na2 SO3), 15 g/L.
2.	 Connect the pre-amplifier (see Chapter 6.3) and perform 2 cycles of short cleaning
(see Chapter 7.13).
3.	 Remove the Sensor from the solution and rinse the residual sodium sulfite with deionised
water.
4.	 Perform the Sensor Unit Verification procedure (see Chapter 10.5).
10.3.2	 Dencytee Sensors
1.	 After decontamination, emerse the Dencytee Sensor for 30 minutes in 1% (v/V) acetic acid
and afterwards 30 min in 1% (w/v) NaOH at room temperature.
2.	 Rinse the Sensor with deionized water.
3.	 Perform the Sensor Unit Verification procedure (see Chapter 10.5).
10.4	 Yearly Maintenance
1.	 Perform the Weekly Maintenance.
2.	 Perform the Sensor Verification procedure.
3.	 Export the temporary data (see Chapter 9.6.3).
4.	 Delete the temporary files (see Chapter 9.6.2).
5.	 Update the Control Unit with the latest Software Update (see Chapter 9.7.1).
MAINTENANCEMAINTENANCE
81Cell Density Monitoring System Operating Instructions
MAINTENANCEMAINTENANCE
10.5	 Sensor Unit Verification
NOTE: Verification just available on screen if Cell Density Integration Kit option not
activated.
A report of the verification is automatically created and stored (explorer or local hard disk). All
tolerances and acceptance criteria are shown in the report.
10.5.1	 Verification of an Incyte Sensor Unit
NOTE: The Capacitance Simulator (Ref 243743) and Hamilton 12880 μS/cm Conductivity
Standard (Ref 238988), formerly called Solution A, as well as a thermometer are
needed to perform the Sensor Unit Verification.
1.	 Connect the Incyte Unit, power on the Control Unit and equilibrate for at least 30 minutes
prior to starting the Verification procedure.
2.	 Go to the Main Menu and enter the Calibration Menu (Figure 13). Select the Verify / Check
Unit Calibration to start the Verification procedure.
3.	 Select the channel. The Sensor type is automatically detected.
4.	 Enter the user name and press start.
5.	 Connect the Capacitance Simulator and press Test Pre-amplifier Calibration to run the
Verification procedure (Figure 51). The successful Verification is indicated. If this procedure
fails it is recommended to perform a calibration.
Figure 51: Testing the pre-amplifier calibration.
6.	 Connect the Incyte Sensor to the Pre-amplifier.
7.	 Select the Manual temperature correction mode. The Automatic mode is disabled.
8.	 Pour Hamilton 12880 μS/cm Conductivity Standard in a clean beaker. Make sure the sensor
mounting instructions are followed (Figure 22).
9.	 Measure the temperature of the Standard with an external thermometer. Enter the conductivity
value according to the temperature correction table on the bottle of the Conductivity Standard.
10.	Press Test Sensor Calibration to carry on with the Verification (Figure 52). The successful
Verification is indicated. If this procedure fails it is recommended to perform a calibration
(see Chapter 10.6.1).
Figure 52: Checking the Sensor calibration.
10.5.2	 Verification of a Dencytee Sensor Unit
ATTENTION! Make sure that the Val/Cal Solution (Ref 243886) is not expired
and avoid contamination or dilution. Mix well prior to usage.
1.	 Connect a Dencytee Sensor Unit and power on the Control Unit. Equilibrate the Val/Cal
Solution (Ref 243886) at 23°C +/- 1°C for at least 30 min prior to starting the Verification
procedure.
83Cell Density Monitoring System Operating Instructions
2.	 Go to the Main Menu and enter the Calibration Menu (Figure 13). Select the Verify / Check
Unit Calibration to start the Verification procedure.
3.	 Select the channel. The Sensor type is automatically detected.
4.	 Enter the user name and press start.
5.	 Make sure that the light path is clean, dust-free and placed in the dark. Press Measure in air
to run the Verification procedure (Figure 53).
6.	 Insert the sensor directly in the bottle of the Val/Cal solution. Make sure that the light path is
well immersed and placed in the dark. Press Measure in solution (Figure 53). The successful
Verification is indicated. If this procedure fails it is recommended to perform a calibration
(see Chapter 10.6.2).
Figure 53: Testing the pre-amplifier calibration.
10.6	 Calibration of a Sensor Unit
NOTE: Calibration just available on screen if Cell Density Integration Kit option not
activated.
Calibration of a Sensor Unit is required if the Sensor Unit Verification did not passed success-
fully.
MAINTENANCEMAINTENANCE
10.6.1	 Calibrate an Incyte Sensor Unit
The Calibration procedure of an Incyte Sensor Unit is a five-step guided procedure. It takes
about an hour to perform this procedure. The Val/Cal Kit for Standard Sensors (Ref 243740),
Low Conductivity "LC" (Ref 243713) or High Conductivity "HC" (Ref 243714) is needed to perform
a Incyte Sensor Unit Calibration.
ATTENTION! Do not disconnect the Unit from the Arc View Controller, or the
Sensor from the pre-amplifier during the Calibration procedure.
NOTE: Make sure to prepare following equipment in addition to the Val/Cal Kit:
stirrer-plate, thermometer
1.	 Connect the Incyte Unit, power on the Control Unit and equilibrate for at least 30 minutes
prior to starting the Calibration procedure.
2.	 Go to the Main Menu and enter the Calibration Menu (Figure 13). Select Calibrate Unit to run
the Calibration procedure.
3.	 Select the channel. The Sensor type is automatically detected.
4.	 Enter the user name and press start (Figure 54).
Figure 54: Start of the Sensor Unit calibration.
85Cell Density Monitoring System Operating Instructions
5.	 Connect the Capacitance Simulator and press Test Pre-amplifier Calibration to proceed
with the Calibration procedure. The successful test is indicated. If this procedure fails it is
recommended to contact the local representative.
6.	 Replace the Simulator with the Incyte Sensor and press Measure in air to calibrate at zero
conductivity. If this procedure fails it is recommended to contact the local representative.
7.	 Select Manual temperature correction mode. The Automatic mode is disabled.
8.	 Pour Hamilton 12880 μS/cm Conductivity Standard in a clean beaker. Make sure the sensor
mounting instructions are followed (Figure 22).
9.	 Measure the temperature of the Standard with an external thermometer. Enter the conductivity
value according to the temperature correction table on the bottle of the Conductivity
Standard.
10.	Press Calibrate sensor constant to start the Calibration (Figure 55). The successful Calibration
is indicated. If this procedure fails it is recommended to contact the local representative.
Figure 55: Calibration of the Sensor constant.
11.	Rinse the Sensor with deionized water.
12.	Pour 150 mL deionized water in a clean beaker. In the following steps 1 to 30 mL of Solution
B is added gradually to the deionized water. Press start (Figure 56).
13.	Add the required amount of Solution B. Press Start.
MAINTENANCEMAINTENANCE
Figure 56: Incyte Sensor calibration.
15.	The successful Calibration is indicated. If this procedure fails it is recommended to contact
the local representative.
16.	Choose the directory, where the Calibration Report should be stored and press Generate
Calibration Report (Figure 57). The valid calibration is automatically saved in the memory of the
Pre-amplifier.
Figure 57: Generation of a Calibration Report.
87Cell Density Monitoring System Operating Instructions
MAINTENANCEMAINTENANCE
10.6.2	 Calibrate a Dencytee Sensor Unit
ATTENTION! Make sure that the Val/Cal Solution (Ref 243886) is not expired
and avoid contamination or dilution. Mix well prior to usage.
1.	 Connect a Dencytee Sensor Unit and power on the Control Unit. Equilibrate the Val/Cal
Solution (Ref 243886) at 23°C +/- 1°C for at least 30 min prior to starting the Calibration
procedure.
2.	 Go to the Main Menu and enter the Calibration Menu (Figure 13). Select Calibrate Unit to run
the Calibration procedure.
3.	 Select the channel. The Sensor type is automatically detected.
4.	 Enter the user name.
5.	 Make sure that the light path is clean, dust-free and placed in the dark. Press Measure in air
to proceed with the Calibration procedure (Figure 53).
6.	 Insert the sensor directly in the bottle of the Val/Cal solution. Make sure that the light path is
well immersed and placed in the dark. Press Measure in solution (Figure 53).
7.	 The successful Calibration is indicated. If this procedure fails it is recommended to contact
the local representative.
8.	 Choose the directory, where the Calibration Report should be stored and press Generate
Calibration Report (Figure 57). The valid calibration is automatically saved in the memory of the
Pre-amplifier.
10.7	 Transfer a Calibration Files to the Arc View Controller
Upon request the calibration of a new Sensor Unit may be done at Hamilton. This new Unit is
shipped with the calibration data on an USB stick. It is possible to transfer this Calibration File to
the Arc View Controller without connecting the Sensor Unit.
1.	 Go to the Main Menu and enter the Calibration Menu (Figure 13). Select Load Calibration
Files (Figure 58).
2.	 Connect the USB stick to the Control Unit (Figure 6, 7, 8) and choose the directory. Press
Load file.
Figure 58: Upload of calibration files.
10.7.1	 Clean and Back Up the Arc View Controller
To keep the storage space of the Control Unit free, the temporary data may be transferred to an
USB stick and deleted.
1.	 Export the temporary data (see Chapter 9.6.3).
2.	 Delete the temporary files (see Chapter 9.6.2).
10.7.2	 Update the Control Unit
1.	 Please check our Homepage www.hamiltoncompany.com/arc_view_update for recent
updates at least once a year.
2.	 Update the Control Unit (see Chapter 9.7.1 and 9.7.2).
89Cell Density Monitoring System Operating Instructions
11	Troubleshooting
11.1	 Self-Diagnostic of the Sensor Unit
The Sensor Unit is running a self-diagnosis continuously. The Status Indicator on the Control
Unit (Figure 12). and the Incyte Status LED on the Incyte Pre-amplifier (Figure 9) report direct
feedback.
The feedback output is similar to a traffic light. A green Status Indicator means everything works
as excepted. An orange Status Indicator shows a warning, that the measurement may be not
correct. A red indication gives an error that the system has stopped working (Table 1).
Table 1: Status Table.
11.2	 Re-Set the Factory Settings
It is possible to re-set the factory settings, in case of Software failure. See Chapter 9.7.2.
11.3	 Verification and Calibration Error
11.3.1	 Verification Error
It is recommended to perform a Calibration (Chapter 10.6), if the Verification of an Incyte or
Dencytee Unit fails (see Chapter 10.5).
11.3.2	 Calibration Error
If a calibration of a Dencytee Unit fails (see Chapter 10.6.2) re-start the Control Unit and perform
a Calibration again. Please contact your local representative, if the Calibration fails again after
re-start of the Controller.
Please contact your local representative, if the Calibration of an Incyte Unit fails.
TROUBLESHOOTINGTROUBLESHOOTING
Notification on the	 Status	 Status LED	 Description	
Control Unit	 Indicator	 (Incyte)
SENSOR: ok	 Green	 Green	 The Sensor Unit is working as
			 expected.
Simulator connected	 Green	 Green	 The permittivity simulator is
			 connected to the Pre-amplifier.
SENSOR: cleaning state	 Green	 Flashing green	 The Pre-amplifier is applying a cleaning
			 cycle, no measurement available.
AMPLIFIER: temperature 	 Green	 Flashing green	 The Pre-amplifier temperature is
security activated			 too high, the security fuse is
			 activated, no measurement available.
SENSOR: Out of range	 Green	 Flashing green	 The measurement is out of range.
measurement	
AMPLIFIER: not found 	 Gray	 Green	 No communication between Sensor
			 Unit and Arc View Controller, please
			 contact the local representative.
SENSOR: sterilization 	 Orange	 Orange	 The Sensor has been sterilized more
count overflow			 than 100 times. Please contact your
			 local representative.
AMPLIFIER : total 	 Orange	 Orange	 The Pre-amplifier has been used
duration of use overflow			 more than 6 months continuously.
			 Please perform a verification.
SENSOR: invalid 	 Orange	 Orange	 The current sensor has not been
sensor calibration			 calibrated with the Pre-amplifier.
Notification on the	 Status	 Status LED	 Description	
Control Unit	 Indicator	 (Incyte)
SENSOR: invalid	 Orange	 Orange	 The sensor serial number is not valid.
calibration	
SENSOR: total	 Orange	 Orange	 The sensor has been used more than
duration overflow			 6 months continuously. Please
			 perform a verification.
SENSOR: out of 	 Orange	 Orange	 The measurement is out of the
calibration range			 calibration range.
SENSOR: not found	 Red	 Red	 No Sensor connected to the
			 Pre-amplifier.
BOX: not connected	 Grey	 Grey	 ComBox is not connected to the PC.
91Cell Density Monitoring System Operating Instructions
11.4	 Getting Technical Support
If any troubles are observed, which you cannot solve based on this operation instruction,
please contact Hamilton Technical Support for further help: techsupport.pa.ch@hamilton.ch
or the local Hamilton representative.
For diagnosis of the measurement loop further data may be needed, it is explained in chapters
11.4.1 to 11.4.3 to get the relevant information.
11.4.1	 Create Print Screens
NOTE: Integrated print screen functionality only available with Arc View Controller.
It is possible to create print screens on certain unexpected events.
1.	 Press the Print Screen Button in the Run Menu (Figure 11) The image is automatically
recorded in the home directory.
2.	 Transfer the Screen Shoot to a USB stick (see Chapter 7.10 and 8.10).
11.4.2	 System Information
The System information gives information about the current Soft- and Hardware version of the
complete Measuring Loop.
1.	 Press the About Button in the Main Manu (Figure 13) and press print screen (Figure 59).
2.	 Transfer the Screen Shoot to a USB stick (see Chapter 7.10 and 8.10).
TROUBLESHOOTINGTROUBLESHOOTING
Figure 59: General information menu of the Arc View Controller.
11.4.3	 System Data
The log files contain the relevant information about the measurement loop, including error
codes. Please send the log files to the technical support for trouble shooting.
See Chapter 9.6.1
11.5	 Remove Corrupt Files
In case of Software failure the deletion of corrupt files may be required.
NOTE: Connect a keyboard to the USB connector of the Arc View Controller prior to
system start.
NOTE: A USB stick is required to remove the files from the Arc View Controller and
store them for safety reasons, to ensure minimal loss of data.
93Cell Density Monitoring System Operating Instructions
ATTENTION! Remove the files stepwise and run a system start after each step
to confirm if the Software is working stable:
•	 move temporary files (stored culture files and calibration/verification reports)
•	 move log files (recent/running culture files)
•	 move configuration files (all configuration data and settings) - this requires the
	 new set up of channel initialization, the OPC or 4- 20 mA communication
1.	 Switch on the Arc View Controller
2.	 Select Evo Recovery with the arrow key, as soon as the possibility is displayed, the software
will now boot in the recovery mode and shows a selection menu.
3.	 Select possibility «4. File recovery» by typing 4 and confirming with the enter key
4.	 Enter the administrator password and confirm with enter. A new selection menu will be
shown.
5.	 Select the possibilities stepwise and confirm with enter, by skipping step 4 as, as this is only
required to transfer error-logs (please see chapter 11.6).
6.	 Wait until the procedure is finished and the system has re-started
7.	 In case the Software is not running stabile perform the next step
NOTE: The data is saved on the USB stick in the folder: Arc-View_backup
11.6	 Transfer Error-Log-Files from the Arc View Controller
If case the Software fails a tool will create Error-Log-Files. These files are relevant information for
the Technical Support.
NOTE: Connect a keyboard to the USB connector of the Arc View Controller prior to
system start.
TROUBLESHOOTINGTROUBLESHOOTING
NOTE: A USB stick is required to remove the files from the Arc View Controller.
1.	 Switch on the Arc View Controller.
2.	 Select Evo Recovery with the arrow key, as soon as the possibility is displayed, the software
will now boot in the recovery mode and shows a selection menu.
3.	 Select possibility «4. File recovery» by typing 4 and confirming with the enter key.
4.	 Enter the administrator password and confirm with enter. A new selection menu will be
shown.
5.	 Select «4: Move crash reports» by typing 4 and confirming with the enter key.
6.	 Wait until the procedure is finished and the system has re-started.
7.	 Remove the USB drive. The error report is now located on the USB drive and is a file ending
with «.dmp», like «interface_Biomass_rack_V2.exe.X.dmp», whereas X may be a 4 or 5 digit
number. There may be up to two files like this.
11.7	 Returning a System for Repair
Please contact Hamilton Technical Support for return of the Instrument. You have to assign a
RGA-number clearly written on the packaging. Please be aware that we are not responsible
for any damages during transportation, if the material is not shipped in original wrapping and
suitable protected.
NOTE: Please ensure that the material is properly decontaminated prior to wrapping.
95Cell Density Monitoring System Operating Instructions
12	 Connection of the Control Unit
	 to the Process Control System
In this chapter it is explained, how to connect the Control Unit to the process control system by:
•	 Modbus communication
•	 OPC communication
•	 4-20 mA Output signal
12.1	 Connect by Modbus communication
12.1.1	 Preparation of the Control Unit
NOTE: The Control Unit acts as Modbus slave (Modbus server), all cell density
measurements can be read by a Modbus master (client). The present implementation of
the Modbus protocol uses the RTU mode over a RS-485 serial line.
1.	 Enter the Advanced Settings and select the Modbus Configuration
Figure 60: Menu for Modbus Configuration.
2.	 Connect an open-end M12 cable to the Modbus Connector (Figure 61) on the right side of
the Controller and configure the Modbus parameters (Figure 60).
Pin	Function
1	 +24 V
2	 RS-485 A
3	GND
4	 RS-485 B
5	n.c.
2
4
31
5
Figure 61: Female M12 connector description.
12.1.2	 Preparation of the Modbus Connection
12.1.2.1	 Definition of the Return Time
The return time depends on the type of Modbus requests.
Modbus request	 Reading	 Writing one register or multiple	 Writing the same register or
		 registers once (next write must 	 the same multiple registers
		 be on different registers)	 twice in a row
Return time	 200ms	 200ms	 1s
Table 2: Modbus return time.
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
97Cell Density Monitoring System Operating Instructions
12.1.2.2	 Overview of the Available Data
The Control Unit provides for each channel three sets of information on Modbus.
Standard cell density data:
•	 Sensor SN
•	 Amplifier SN
•	 Amplifier Status (integer type format)
•	Conductivity
•	 Cell density
•	 Measure index
•	 Amplifier status (float type format)
Incyte Scan data:
•	 Delta Epsilon
•	Fc
•	Alpha
•	 Fscan Epsilon (not available with standard licenses)
•	 Spectro index
Capacitance at all frequencies of the Incyte Scan:
•	 Fscan - Index
•	 Scan frequencies list (frequency)
•	 Scan C(f) list (permitivity)
Each one of the three data sets (standard cell density, Incyte Scan data, permittivity at all
frequencies of the Incyte Scan) has its own index which is incremented at each valid measurement.
An index increment stopped for a long time means there is no communication between
the Arc View Controller and amplifier, e.g. if the pre-amplifier is not connected.
To preserve data integrity, the Modbus master must request the entire data set in only one
frame. The system guarantees the data set index tallies with only one measurement.
NOTE: If the master reads data separately with an undefined time between requests,
the Arc View Controller could change registers during this time and so the index could
not tally with only a single measurement.
12.1.2.3	 Definition of 32 Bit Data Type
The Modbus protocol defines registers of 16 bit words. The registers are transmitted in big-endian
format. The float values and unsigned long values are 32 bit sized; therefore they are mapped
into two registers. These 32 bit values are transmitted low word (16 bit) first whereas the bytes
inside the words are sent with big-endian format.
For example:
•	 Floating point representation: 97.18467
•	 Hexadecimal representation 0x42C25E8D (IEEE 754 Standard)
•	 The float value in the register table:
Registers	Value
86	0x5E8D
87	0x42C2
Table 3: Register Values.
Modbus answer frame (slave address = 1):
• 0x01 | 0x04 | 0x5E | 0x8D | 0x42 | 0xC2 | 0xC9 | 0x76
Activate the byte swap option in the Modbus configuration screen allow to swap the byte in the
word. The answer frame with this option is:
•	 0x01 | 0x04 | 0x8D | 0x5E | 0xC2 | 0x42 | 0xC9 | 0x76
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
99Cell Density Monitoring System Operating Instructions
12.1.2.4	 Definition of Modbus Exceptions
If the slave cannot serve a request, it returns an exception with the following error codes:
Error code	 Name	 Description
0x01	 Illegal Function	 The function code received in the query is not an
		 allowable action for the slave.
0x02	 Illegal Data Address	 The data address received in the query is not an
		 allowable address for the slave.
0x03	 Illegal Data Value	 A value contained in the query data field is not an
		 allowable value for the slave.
0x06	 Slave Device Busy	 The slave is engaged in processing a long-duration
		 program command and transmits the message later
		 when the slave is free.
Table 4: Definitions of Modbus Exceptions.
12.1.2.5	 Pre-Amplifier Status Codes
The following table lists the possible status code for Modbus registers amplifier status.
Table 5: Pre-amplifier Status Code.
NOTE: Some status codes are only available for Incyte Sensors.
Status	 Code	 Amplifier led	 Description
		indicator
SENSOR: not found	 0	 Red	 No sensor connected to the Pre-amplifier.
SENSOR: sterilization 	 1	 Orange	 The sensor has been sterilized more
count overflow			 than 100 times. Please contact the
			 local representative.
AMPLIFIER : total duration 	 4	 Orange	 The preamplifier has been used more
of use overflow			 than 1 year continuously. Please
			 perform a Pre-amplifier check.
PROBE: invalid sensor	 5	 Orange	 The current sensor has not been
calibration 			 calibrated with the Pre-amplifier.
SENSOR: invalid calibration	 6	 Orange	 The current sensor has not been
			 calibrated with the Pre-amplifier.
			 The sensor serial number is not valid.
SENSOR: total duration	 7	 Orange	 The sensor has been used more than
overflow 			 6 months continuously. Please perform
			 a verification.
PROBE: out of calibration	 8	 Orange	 The measurement is out of the
range			 calibration range.
PROBE: cleaning state	 9	 Flashing green	 The Pre-amplifier is applying specific
			 voltage to clean the sensor, no
			 measurement available.
AMPLIFIER: temperature 	 10	 Flashing green	 The Pre-amplifier temperature is too
security activated			 high, the security fuse is activated, no
			 measurement available.
PROBE: Out of range 	 11	 Flashing green	 The measurement is out of range.
measurement
PROBE: ok	 12	 Green	 The unit is working as expected.
Biomass simulator connected	 13	 Green	 The Simulator is connected to the
			 Pre-amplifier.
SENSOR: total sterilization 	 14	 Orange	 The automatic sterilization counter has
time overflow			 reached the maximum value (100).
SENSOR: overtime 	 15	 Orange	 The sensor has been used more than
reached-calib/check needed			 1 year since the last calibration. Please
			 perform a calibration.
AMPLIFIER: overtime 	 16	 Orange	 The Pre-Amplifier has been used more
reached/check needed			 than 1 year since the last calibration
			 Please perform a calibration.
AMPLIFIER: not found 	 17	 Off	 No Pre-Amplifier is connected to the
			 channel.
bootloader	 18	 Red flashing	 Pre-Amplifier firmware updating.
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
101Cell Density Monitoring System Operating Instructions
12.1.2.6	 Calibration and Validation via Modbus
Dencytee and Incyte units can be calibrated and validated via Modbus if the Cell Density Integration
Kit mode is enabled (see Chapter 9.2.6).
Since calibration and validation are both procedures with multiple steps involved, an individual
state machine that can be controlled via Modbus is used for the four use cases:
•	 Calibration of Incyte Unit
•	 Validation of Incyte Unit
•	 Calibration of Dencytee Unit
•	 Validation of Dencytee Unit
12.1.2.6.1		 General Concepts
12.1.2.6.1.1	 Controlling the State Machine via Modbus
The state machine of one measurement channel can be controlled using four Modbus registers.
•	 Parameter: Register for passing an input argument to the state machine (e.g. nominal
conductivity of currently used calibration standard)
•	 Command: Register for command codes that trigger state transitions in the state machine.
•	 Acknowledge: Status register controlled by the software in order to signal the state of
processing the command.
•	 State: Register that returns the current state of the state machine
A state transition involves the following typical steps:
•	 Modbus Master writes the parameter (if required)
•	 Modbus Master writes the command; the software confirms reception of the command by
setting Acknowledge to 0.
•	 Modbus Master resets the command to 0 in order to start execution of the command in
the software.
•	 Once execution of a command is finished, the software writes the new state to the State
register and resets the Acknowledge register to 1 in order to indicate readiness for a new
command.
Parameter
Modbus Master
Command
Modbus Master	
0
Acknoledge
Control Unit	
0
State
Control Unit
COMMAND
EXECUTION
IN BIOMASS
NEW VALUE P
NEW VALUE S
NEW VALUE A
Default value for P is 0 / Default value for A is 1
NEW VALUE C
1
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
103Cell Density Monitoring System Operating Instructions
The main state machine for every single measurement channel looks as depicted in this chapter.
If CDIK mode is switched off when starting the software, the state machine will transition automatically
to state 8 “not supported”. If CDIK (Cell Density Integration Kit) mode is switched on
when starting the software, the state machine will be in state 1 “Initial”.
At any time only one calibration or validation process on one channel can be active. As soon
as a calibration or validation on any measurement channel is started, the state machines of the
other channels will automatically transition to state 9 “Busy with another channel”.
Every calibration / validation has a specific sub-state machine that will be described in chapters
12.1.2.6.2 – 12.1.2.6.5.
When the calibration or validation is completed the state machine of the corresponding measurement
channel will be in state 2 “Final”. After resetting the state machine to state 1 “Initial”
the state machines of the other measurement channels will transition automatically from state 9
“Busy” to 1 “Initial”.
State	 Command	 Next State	 Description
1	 1	 10	 Start a calibration or validation. The command code is
			 always 1, the sensor type and the action (validation or
			 calibration) is specified by selecting the corresponding
			 Modbus register (see Chapter 12.1.2.6.1.3). Make sure
			 the sensor is connected to the Pre-amplifier
2	 2	 1	 Reset the state machine to the initial state in order to
			 resume normal operation
8	 –		 Not Supported: CDIK mode is switched off
9	 –		 Busy: A calibration/validation is active on another
			measurement channel
12.1.2.6.1.3	 Modbus registers for CDIK, Arc View Controller 265 and
		 PC software
The following table defines the Modbus registers used for controlling the state machines for the
two measurement channels depending on the type of sensor and action (calibration or validation).
Note that Parameter and Command have to be read and written as Modbus Holding Registers
(Modbus function codes 0x03 and 0x10). Acknowledge and State have to be read as Modbus
Input Registers (Modbus function code 0x04).
12.1.2.6.1.2	 The main state machine
State 8 CDIK
not supported
CDIK not supported
CDIKsupported
State 1 Initial
Statemachine
«Calibration Incyte»
Statemachine
«Validation Incyte»
Statemachine
«Calibration Dencytee»
Statemachine
«Validation Dencytee»
Command1
Command1
Command1
Command1
Command2
State 2 Final
State 9 Busy with
another channel
Any other channel
is not in State Initial
All other channels
are in State Initial
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
105Cell Density Monitoring System Operating Instructions
	 Parameter	 Command	 Acknowledge	State
	 (Holding Reg.)	 (Holding Reg.)	 (Input Reg.)	 (Input Reg.)
Channel 3
Validation Incyte	 44	 43	 130	 131
Calibration Incyte	 46	 45	 132	 133
Validation Dencytee	 114	 113	 824	 825
Calibration Dencytee	 116	 115	 826	 827
Channel 4
Validation Incyte	 60	 59	 176	 177
Calibration Incyte	 62	 61	 178	 179
Validation Dencytee	 130	 129	 871	 872
Calibration Dencytee	 132	 131	 873	 874
12.1.2.6.1.5	 General error states
During calibration and validation errors will be indicated by a transition to an error state. Error
states have a state number of 900 and above. With command 3 a transition to state 2 “Final”
is possible.
In any state if an invalid command is invoked the state machine transitions to state 999 “Invalid
command”.
State	 Command	 Next State	 Description
9xx	 3	 2	 States numbered from 900 to 999 are error states. The
			 command 3 “Cancel” transitions from any error state to
			 the state 2 “Final”. Some error states allow repeating the
			 step that has led to the error. This will be mentioned in
			 the corresponding error state description
900	 3	 2	 Wrong sensor type
997	 3	 2	 Error while resetting the sensor or preamplifier
998	 3	 2	 Generic error while communicating with the
			 sensor / preamplifier
999	3	2	Invalid command
	 Parameter	 Command	 Acknowledge	State
	 (Holding Reg.)	 (Holding Reg.)	 (Input Reg.)	 (Input Reg.)
Channel 1
Validation Incyte	 12	 11	 38	 39
Calibration Incyte	 14	 13	 40	 41
Validation Dencytee	 44	 43	 388	 389
Calibration Dencytee	 46	 45	 390	 391
Channel 2
Validation Incyte	 28	 27	 84	 85
Calibration Incyte	 30	29	 86	87
Validation Dencytee	 60	 59	 435	 436
Calibration Dencytee	 62	 61	 437	 438
12.1.2.6.1.4	 Modbus registers for Arc View Controller 465
The following table defines the Modbus registers used for controlling the state machines for the
four measurement channels depending on the type of sensor and action (calibration or validation).
Note that Parameter and Command have to be read and written as Modbus Holding Registers
(Modbus function codes 0x03 and 0x10). Acknowledge and State have to be read as Modbus
Input Registers (Modbus function code 0x04).
	 Parameter	 Command	 Acknowledge	State
	 (Holding Reg.)	 (Holding Reg.)	 (Input Reg.)	 (Input Reg.)
Channel 1
Validation Incyte	 12	 11	 38	 39
Calibration Incyte	 14	 13	 40	 41
Validation Dencytee	 82	 81	 730	 731
Calibration Dencytee	 84	 83	 732	 733
Channel 2
Validation Incyte	 28	 27	 84	 85
Calibration Incyte	 30	29	 86	87
Validation Dencytee	 98	 97	 777	 778
Calibration Dencytee	 100	 99	 779	 780
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
107Cell Density Monitoring System Operating Instructions
12.1.2.6.2.1	 Table of states
State	 Command	 Next State	 Description
10	 10	 20	 Temperature stabilization of the preamplifier
20			 User has to connect the Incyte simulator to the
			preamplifer
20	 20	 30	 Calibration check of the preamplifier
30			 User disconnects the Incyte simulator and connects the
			 Incyte sensor. The sensor is immersed in solution A.
			 The conductivity of solution A at the current temperature
			 has to be determined using the table on the bottle
30	 30	 2	 Check sensor calibration
			 Parameter: conductivity of solution A at current
			temperature
			 The conductivity has to be written as a 16 Bit integer value
			 in the unit µS/cm to the corresponding Modbus register
2			 The user removes the sensor from Solution A
12.1.2.6.2.2	 Error states
State	 Command	 Next State	 Description
901	 3	 2	 Error while reading the preamplifier’s temperature
902	 3	 2	 Preamplifier temperature is out of specified range
903	 3	 2	 Preamplifier temperature is not stable
904	 3	 2	 Preamplifier calibration is invalid
905	 3	 2	 Sensor/Preamplifier is not calibrated
920	 3	 2	 Preamplifier/sensor calibration is invalid
12.1.2.6.2		 Validation Incyte Unit
No Incyte sensor connected
State 10 Ready for
temperature stabilization
Failure
Execute preamplifier reset
Execute temperature
stabilization
Error states according table
Failure
State 20 Ready for check
preamplifier calibration
User connects the simulator
Preamplifier calibration
check is performed
Failure
State 30 Ready for check
sensor calibration
Sensor calibration check
is performed
User disconnects the simulator
User connects the sensor and
immerses it in solution A
Failure
Failure
Execute preamplifier reset
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
109Cell Density Monitoring System Operating Instructions
12.1.2.6.3.1	 Table of states
State	 Command	 Next State	 Description
10	 10	 20	 Temperature stabilization of the preamplifier.
			 Acknowledge: The type of calibration is returned
			 1 = Standard
			 2 = High conductivity
			 3 = Low conductivity
20	 20	 30	 Initialize Preamplifier
30			 User has to connect the Incyte simulator to the
			preamplifer
30	 30	 40	 Calibration check of the preamplifier
40			 User disconnects the Incyte simulator and connects the
			Incyte sensor
40	 40	 50	 Calibrate zero conductivity in air
50			 The sensor is immersed in solution A. The conductivity
			 of solution A at the current temperature has to be
			 determined using the table on the bottle
50	 50	 110	 Calibration of cell constant
			 Parameter: conductivity of solution A at current
			temperature
			 The conductivity has to be written as a 16 Bit integer value
			 in the unit µS/cm to the corresponding Modbus register
110			 The user immerses the sensor in de-ionized water and adds
			 solution B according the calibration type (see 12.1.2.6.3.2)
110	 110	 120	 Calibration of dilution step 1.
			 If this step fails, it can be repeated (see 12.1.2.6.3.3)
120			 User adds solution B according the calibration type
			(see 12.1.2.6.3.2)
120	 120	 130	 Calibration of dilution step 2.
			 If this step fails, it can be repeated (see 12.1.2.6.3.3)
130			 User adds solution B according the calibration type
			(see 12.1.2.6.3.2)
			…
180			 User adds solution B according the calibration type
			(see 12.1.2.6.3.2)
180	 180	 200	 Calibration of dilution step 8.
			 If this step fails, it can be repeated (see 12.1.2.6.3.3)
200			 The user removes the sensor from Solution B
200	 200	 2	 Calibration file is created
12.1.2.6.3		 Calibration Incyte Unit
No Incyte sensor connected
State 10 Ready for temperature stabilization
Failure
Execute preamplifier reset
Execute temperature
stabilization
Error states according table
Failure
State 20 Ready for preamplifier initialization
Failure
State 30 Ready for preamplifier calibration check
Preamplifier calibration
check is performed
User connects
the simulator
Failure
Failure
Create calibration file
State 40 Ready for zero conductivity calibration
Zero conductivity
calibration is performed
User disconnects the simulator
and connects the sensor
State 50 Ready for cell constant calibration
Cell constant calibration
is performed
Failure
State 1n0 Ready for dilution step n
User immerses the
sensor in solution A
User immerses the
sensor in dilution n
Failure
State 200 Ready for creating calibration file
n<=8
n=n+1
Failure
Execute preamplifier reset
Failure
Cancel
Cancel
States 91n Failure in
dilution step n
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
111Cell Density Monitoring System Operating Instructions
12.1.2.6.3.3	 Error states
State	 Command	 Next State	 Description
901	 3	 2	 Error while reading the preamplifier’s temperature
902	 3	 2	 Preamplifier temperature is out of specified range
903	 3	 2	 Preamplifier temperature is not stable
904	 3	 2	 Preamplifier is not calibrated or calibration is invalid
905	 3	 2	 Error while initializing sensor
906	 3	 2	 Error while calibrating zero conductivity
907	 3	 2	 Conductivity out of range
908	 3	 2	 Calibration in conductivity standard failed
909	 3	 2	 Failure in dilution step 1. Incyte calibration can be
	 	 cancelled with command 3. Dilution step can be
	 4	 110	 repeated with command 4
910	 3	 2	 Failure in dilution step 2. Incyte calibration can be
	 	 cancelled with command 3. Dilution step can be
	 4	 120	 repeated with command 4
911	 3	 2	 Failure in dilution step 3. Incyte calibration can be
	 	 cancelled with command 3. Dilution step can be
	 4	 130	 repeated with command 4
912	 3	 2	 Failure in dilution step 4. Incyte calibration can be
	 	 cancelled with command 3. Dilution step can be
	 4	 140	 repeated with command 4
913	 3	 2	 Failure in dilution step 5. Incyte calibration can be
	 	 cancelled with command 3. Dilution step can be
	 4	 150	 repeated with command 4
914	 3	 2	 Failure in dilution step 6. Incyte calibration can be
	 	 cancelled with command 3. Dilution step can be
	 4	 160	 repeated with command 4
915	 3	 2	 Failure in dilution step 7. Incyte calibration can be
	 	 cancelled with command 3. Dilution step can be
	 4	 170	 repeated with command 4
916	 3	 2	 Failure in dilution step 8. Incyte calibration can be
	 	 cancelled with command 3. Dilution step can be
	 4	 180	 repeated with command 4
917	 3	 2	 Conductivity is not stable
918	 3	 2	 Error while writing calibration file
12.1.2.6.3.2	 Dilution steps for different calibration types
Part of the Incyte calibration is a series of measurements in different conductivity dilutions.
Solution B is added to a beaker with 150ml de-ionized water in 8 steps according the following
table. The dose is specific for the calibration type of the Incyte unit (can be read in state 10,
see Chapter 12.1.2.6.3.1)
	 Addition of Solution B per dilution step [ml]
	 Standard	 High conductivity	 Low conductivity
	 calibration	calibration	 calibration
Dilution step 1	 1	 1	 0.5
Dilution step 2	 1	 2	 1
Dilution step 3	 2	 2	 1.5
Dilution step 4	 5	 5	 2
Dilution step 5	 10	 15	 5
Dilution step 6	 20	 20	 10
Dilution step 7	 20	 30	 10
Dilution step 8	 30	 40	 20
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
113Cell Density Monitoring System Operating Instructions
12.1.2.6.4.1	 Table of states
State	 Command	 Next State	 Description
10	 10	 20	 Temperature stabilization of the preamplifier
20			 Make sure the light path is clean, dust-free and dark
20	 20	 30	 Measurement in air
30			 User immerses the sensor in the Val/Cal Solution OD
30	 30	 2	 Measurement in Val/Cal Solution
2			 User removes the sensor from Val/Cal Solution
12.1.2.6.4.2	 Error states
State	 Command	 Next State	 Description
920	 3	 2	 Error while reading the preamplifier’s temperature
921	 3	 2	 Preamplifier temperature is out of specified range
922	 3	 2	 Preamplifier temperature is not stable
923	 3	 2	 External interference light detected
928	 3	 2	 Error while measuring in air
929	 3	 2	 Error while measuring in Val/Cal solution
930	 3	 2	 Preamplifier/sensor calibration is invalid
12.1.2.6.4		 Validation Dencytee
No Dencytee sensor connected
State 10 Ready for
temperature stabilization
Failure
Execute preamplifier reset
Execute temperature
stabilization
Error states according table
Failure
State 20 Ready for
measurement in air
Measurement in air
is performed
Failure
State 30 Ready for measurement
in Val/Cal Solution
Measurement in Val/Cal
Solution is performed
Failure
Failure
Execute preamplifier reset
Cancel
User immerses the sensor
in Val/Cal Solution
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
115Cell Density Monitoring System Operating Instructions
12.1.2.6.5.1	 Table of states
State	 Command	 Next State	 Description
10	 10	 20	 Temperature stabilization of the preamplifier
20	 20	 30	 Initialize Preamplifier
30			 Make sure the light path is clean, dust-free and dark
30	 30	 40	 Calibration in air
40			 User immerses the sensor in the Val/Cal Solution OD
40	 40	 50	 Calibration in Val/Cal Solution
50			 User removes the sensor from Val/Cal Solution
50	 50	 2	 Calibration file is created
12.1.2.6.5.2	 Error states
State	 Command	 Next State	 Description
920	 3	 2	 Error while reading the preamplifier’s temperature
921	 3	 2	 Preamplifier temperature is out of specified range
922	 3	 2	 Preamplifier temperature is not stable
923	 3	 2	 External interference light detected
924	 3	 2	 Error while initializing sensor
925	 3	 2	 Error while calibrating in air
926	 3	 2	 Error while calibrating in Val/Cal Solution
927	 3	 2	 Error while writing calibration file
12.1.2.6.5		 Calibration Dencytee
State 40 Ready for calibration in Val/Cal solution
No Dencytee sensor connected
State 10 Ready for temperature stabilization
Failure
Execute preamplifier reset
Execute temperature
stabilization
Error states according table
Failure
State 20 Ready for preamplifier initialization
Failure
State 30 Ready for calibration in air
Calibration in air
is performed
Failure
Failure
Execute preamplifier reset
Calibration in Val/Cal
solution is performed
State 50 Ready for creating calibration file
Failure
Create calibration file
Failure
User immerses the sensor
in Val/Cal Solution
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
117Cell Density Monitoring System Operating Instructions
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
Gateway serial number 	 001	  	 string	 6
Measures - Ch 1	  	  	  	  
Sensor serial number	 007	  	 string	 6
Amplifier serial number	 013	  	 string	 6
Amplifier status	 019	  	 word 16bits	 1
Conductivity 	 020	 mS/cm	 float32	 2
Permittivity	 022	 pF/cm	float32	 2
Measure Index	 024	  	 unsigned long	 2
Amplifier Status - float format	 026	  	 float32	 2
Reserved	 028		 float32	 2
Reserved	 030		 float32	 2
Reserved	 032		 float32	 2
Reserved	 034		 float32	 2
Reserved	 036		 float32	 2
Acknowledge Val. Incyte	 038	  	 word 16bits	 1
State Val. Incyte	 039	  	 word 16bits	 1
Acknowledge Cal. Incyte	 040	  	 word 16bits	 1
State Cal. Incyte	 041	  	 word 16bits	 1
Spectro - Ch 1	  	  	  	  
DeltaEpsilon	 042	 	 float32	 2
Fc	 044	 	 float32	 2
Alpha	 046	 	 float32	 2
FscanEpsilon	 048	 	 float32	 2
Spectro index	 050	  	 unsigned long	 2
Reserved	 052		 Word 16bits	 1
Measures - Ch 2	  	  	  	  
Sensor serial number	 053	  	 string	 6
Amplifier serial number	 059	  	 string	 6
Amplifier status	 065	  	 word 16bits	 1
Conductivity 	 066	 mS/cm	 float32	 2
Permittivity	 068	 pF/cm	float32	 2
Measure Index	 070	  	 unsigned long	 2
Amplifier Status - float format	 072	  	 float32	 2
Reserved	 074		 float32	 2
Reserved	 076		 float32	 2
Reserved	 078		 float32	 2
Reserved	 080		 float32	 2
Reserved	 082		 float32	 2
Acknowledge Val. Incyte	 084	  	 word 16bits	 1
State Val. Incyte	 085	  	 word 16bits	 1
Acknowledge Cal. Incyte	 086	  	 word 16bits	 1
State Cal. Incyte	 087	  	 word 16bits	 1
Spectro - Ch 2	  	  	  	  
DeltaEpsilon	 088	 	 float32	 2
Fc	 090	 	 float32	 2
Alpha	 092	 	 float32	 2
FscanEpsilon	 094	 	 float32	 2
Spectro index	 096	  	 float32 / ul	 2
Reserved	 098	  	 word 16bits	 1
Info-Fscan-Ch1	  	  	  	  
Reserved	 99	  	 word 16bits	 1
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
12.1.2.7	 Modbus Tables Arc View Controller 265, ComBox and
	 Cell Density Integration Kit
12.1.2.7.1 Input Registers Tables (Read only)
12.1.2.7.1.1 Read the Modbus Commands
All measurements and readable information which comes from the sensors are memorized in
the Modbus input registers table. The Modbus function code 0x04 is used to read this table. It
allows reading 1 to 125 contiguous input registers from the Control Unit. The request specifies
the starting address and the number of registers to be read. In the Modbus frame, registers
are addressed starting at zero. For example, input registers numbered 1-16 are addressed as
0-15 inside the frame.
12.1.2.7.1.2 Incyte Input Registers Table
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
119Cell Density Monitoring System Operating Instructions
Reserved	 100	  	 word 16bits	 1
Reserved	 101	  	 word 16bits	 1
index-Fscan-ch1	 102	  	 unsigned long	 2
Freq-FSCAN-Ch1(30 points)	  	  	  	  
Scan-Freq-Pt0	 104	 Hz	 float 32 bits	 2
Scan-Freq-Pt1	 106	 Hz	 float 32 bits	 2
Scan-Freq-Pt2	 108	 Hz	 float 32 bits	 2
Scan-Freq-Pt3	 110	 Hz	 float 32 bits	 2
Scan-Freq-Pt4	 112	 Hz	 float 32 bits	 2
Scan-Freq-Pt5	 114	 Hz	 float 32 bits	 2
Scan-Freq-Pt6	 116	 Hz	 float 32 bits	 2
Scan-Freq-Pt7	 118	 Hz	 float 32 bits	 2
Scan-Freq-Pt8	 120	 Hz	 float 32 bits	 2
Scan-Freq-Pt9	 122	 Hz	 float 32 bits	 2
Scan-Freq-Pt10	 124	 Hz	 float 32 bits	 2
Scan-Freq-Pt11	 126	 Hz	 float 32 bits	 2
Scan-Freq-Pt12	 128	 Hz	 float 32 bits	 2
Scan-Freq-Pt13	 130	 Hz	 float 32 bits	 2
Scan-Freq-Pt14	 132	 Hz	 float 32 bits	 2
Scan-Freq-Pt15	 134	 Hz	 float 32 bits	 2
Scan-Freq-Pt16	 136	 Hz	 float 32 bits	 2
Scan-Freq-Pt17	 138	 Hz	 float 32 bits	 2
Scan-Freq-Pt18	 140	 Hz	 float 32 bits	 2
Scan-Freq-Pt19	 142	 Hz	 float 32 bits	 2
Scan-Freq-Pt20	 144	 Hz	 float 32 bits	 2
Scan-Freq-Pt21	 146	 Hz	 float 32 bits	 2
Scan-Freq-Pt22	 148	 Hz	 float 32 bits	 2
Scan-Freq-Pt23	 150	 Hz	 float 32 bits	 2
Scan-Freq-Pt24	 152	 Hz	 float 32 bits	 2
Scan-Freq-Pt25	 154	 Hz	 float 32 bits	 2
Scan-Freq-Pt26	 156	 Hz	 float 32 bits	 2
Scan-Freq-Pt27	 158	 Hz	 float 32 bits	 2
Scan-Freq-Pt28	 160	 Hz	 float 32 bits	 2
Scan-Freq-Pt29	 162	 Hz	 float 32 bits	 2
C(f)-FSCAN-Ch1(30 points)	  	  	  	  
Scan-C(f)-Pt0	 164	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt1	 166	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt2	 168	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt3	 170	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt4	 172	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt5	 174	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt6	 176	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt7	 178	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt8	 180	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt9	 182	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt10	 184	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt11	 186	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt12	 188	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt13	 190	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt14	 192	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt15	 194	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt16	 196	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt17	 198	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt18	 200	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt19	 202	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt20	 204	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt21	 206	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt22	 208	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt23	 210	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt24	 212	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt25	 214	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt26	 216	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt27	 218	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt28	 220	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt29	 222	 pF/cm	 float 32 bits	 2
Info-Fscan-Ch2	  	  	  	
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
121Cell Density Monitoring System Operating Instructions
Reserved	 224	  	 word 16bits	 1
Reserved	 225	  	 word 16bits	 1
Reserved	 226	  	 word 16bits	 1
index-Fscan-ch2	 227	  	 unsigned long	 2
Freq-FSCAN-Ch2(30 points)	  	  	  	  
Scan-Freq-Pt0	 229	 Hz	 float 32 bits	 2
Scan-Freq-Pt1	 231	 Hz	 float 32 bits	 2
Scan-Freq-Pt2	 233	 Hz	 float 32 bits	 2
Scan-Freq-Pt3	 235	 Hz	 float 32 bits	 2
Scan-Freq-Pt4	 237	 Hz	 float 32 bits	 2
Scan-Freq-Pt5	 239	 Hz	 float 32 bits	 2
Scan-Freq-Pt6	 241	 Hz	 float 32 bits	 2
Scan-Freq-Pt7	 243	 Hz	 float 32 bits	 2
Scan-Freq-Pt8	 245	 Hz	 float 32 bits	 2
Scan-Freq-Pt9	 247	 Hz	 float 32 bits	 2
Scan-Freq-Pt10	 249	 Hz	 float 32 bits	 2
Scan-Freq-Pt11	 251	 Hz	 float 32 bits	 2
Scan-Freq-Pt12	 253	 Hz	 float 32 bits	 2
Scan-Freq-Pt13	 255	 Hz	 float 32 bits	 2
Scan-Freq-Pt14	 257	 Hz	 float 32 bits	 2
Scan-Freq-Pt15	 259	 Hz	 float 32 bits	 2
Scan-Freq-Pt16	 261	 Hz	 float 32 bits	 2
Scan-Freq-Pt17	 263	 Hz	 float 32 bits	 2
Scan-Freq-Pt18	 265	 Hz	 float 32 bits	 2
Scan-Freq-Pt19	 267	 Hz	 float 32 bits	 2
Scan-Freq-Pt20	 269	 Hz	 float 32 bits	 2
Scan-Freq-Pt21	 271	 Hz	 float 32 bits	 2
Scan-Freq-Pt22	 273	 Hz	 float 32 bits	 2
Scan-Freq-Pt23	 275	 Hz	 float 32 bits	 2
Scan-Freq-Pt24	 277	 Hz	 float 32 bits	 2
Scan-Freq-Pt25	 279	 Hz	 float 32 bits	 2
Scan-Freq-Pt26	 281	 Hz	 float 32 bits	 2
Scan-Freq-Pt27	 283	 Hz	 float 32 bits	 2
Scan-Freq-Pt28	 285	 Hz	 float 32 bits	 2
Scan-Freq-Pt29	 287	 Hz	 float 32 bits	 2
C(f)-FSCAN-Ch2(30 points)	  	  	  	  
Scan-C(f)-Pt0	 289	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt1	 291	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt2	 293	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt3	 295	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt4	 297	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt5	 299	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt6	 301	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt7	 303	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt8	 305	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt9	 307	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt10	 309	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt11	 311	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt12	 313	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt13	 315	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt14	 317	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt15	 319	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt16	 321	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt17	 323	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt18	 325	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt19	 327	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt20	 329	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt21	 331	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt22	 333	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt23	 335	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt24	 337	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt25	 339	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt26	 341	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt27	 343	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt28	 345	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt29	 347	 pF/cm	 float 32 bits	 2
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
Table 6: Incyte Input Registers.
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
123Cell Density Monitoring System Operating Instructions
Gateway serial number 	 351	  	 string	 6
Measures - Ch 1	  	  	  	  
Sensor serial number	 357	  	 string	 6
Amplifier serial number	 363		 string	 6
Amplifier status	 369		 word 16 bits	 1
Reserved	 370		 float 32	 2
Biomass	 372		 float 32	 2
Measure Index	 374		 unsigned long	 2
Amplifier Status – float format	 376		 float 32	 2
Reserved	 378		 float 32	 2
Reserved	 380		 float 32	 2
Reserved	 382		 float 32	 2
Reserved	 384		 float 32	 2
Reserved	 386		 float 32	 2
Acknowledge Val. Dencytee	 388		 word 16 bits	 1
State Val. Dencytee	 389		 word 16 bits	 1
Acknowledge Cal. Dencytee	 390		 word 16 bits	 1
State Cal. Dencytee	 391		 word 16 bits	 1
Reserved	 392		 float32	 2
Reserved	 394		 float 32	 2
Reserved	 396		 float 32	 2
Reserved	 398		 float 32	 2
Reserved	 400		 float 32	 2
Reserved	 402		 float 32	 2
Measures - Ch 2	  	  	  	  
Sensor serial number	 404	  	 string	 6
Amplifier serial number	 410		 string	 6
Amplifier status	 416		 word 16 bits	 1
Reserved	 417		 float 32	 2
Biomass	 419		 float 32	 2
Measure Index	 421		 unsigned long	 2
Amplifier Status – float format	 423		 float 32	 2
Reserved	 425		 float 32	 2
Reserved	 427		 float 32	 2
Reserved	 429		 float 32	 2
Reserved	 431		 float 32	 2
Reserved	 433		 float 32	 2
Acknowledge Val. Dencytee	 435		 word 16 bits	 1
State Val. Dencytee	 436		 word 16 bits	 1
Acknowledge Cal. Dencytee	 437		 word 16 bits	 1
State Cal. Dencytee	 438		 word 16 bits	 1
Reserved	 439		 float32	 2
Reserved	 441		 float 32	 2
Reserved	 443		 float 32	 2
Reserved	 445		 float 32	 2
Reserved	 447		 float 32	 2
Reserved	 449		 float 32	 2
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
12.1.2.7.1.3 Dencytee Input Registers Table
Table 7: Dencytee input register.
12.1.2.7.2 Holding Registers (read and write)
12.1.2.7.2.1 Read and Write Function Code
Writing holding registers allow changing configuration and performs several actions.
• Use 0x06 standard function code to write single holding register.
This function code is used to write a single holding register in the Control Unit. The Request
PDU specifies the address of the register to be written. Registers are addressed starting at
zero. Therefore register numbered 1 is addressed as 0. The normal response is an echo of the
request, returned after the register contents have been written.
• Use 0x10 standard function code to write holding registers.
This function code is used to write a block of contiguous registers (1 to 123 registers) in the
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
125Cell Density Monitoring System Operating Instructions
Channel 1				
Mark Zero auto	 1		 word 16bits	 1
Clear Zero auto	 2		 word 16bits	 1
Recepy number Unit Factor	 3		 word 16bits	 1
Recepy number Measure Mode	 4		 word 16bits	 1
Activate Manual Cleaning	 5		 word 16bits	 1
Activate auto Cleaning	 6		 word 16bits	 1
Recepy number Cleaning	 7		 word 16bits	 1
Reserved	 8		 word 16bits	 1
Offset zero	 9	 pF/cm	 Float 32bits	 2
Command Val. Incyte	 11		 word 16bits	 1
Parameter Val. Incyte	 12		 word 16bits	 1
Command Cal. Incyte	 13		 word 16bits	 1
Parameter Cal. Incyte	 14		 word 16bits	 1
Reserved	 15		 word 16bits	 1
Reserved	 16		 word 16bits	 1
Channel 2				
Mark Zero auto	 17		 word 16bits	 1
Clear Zero auto	 18		 word 16bits	 1
Recepy number Unit Factor	 19		 word 16bits	 1
Recepy number Measure Mode	 20		 word 16bits	 1
Activate Manual Cleaning	 21		 word 16bits	 1
Activate auto Cleaning	 22		 word 16bits	 1
Recepy number Cleaning	 23		 word 16bits	 1
Reserved	 24		 word 16bits	 1
Offset zero	 25	 pF/cm	 Float 32bits	 2
Command Val. Incyte	 27		 word 16bits	 1
Parameter Val. Incyte	 28		 word 16bits	 1
Command Cal. Incyte	 29		 word 16bits	 1
Parameter Cal. Incyte	 30		 word 16bits	 1
Reserved	 31		 word 16bits	 1
Reserved	 32		 word 16bits	 1
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
Control Unit. The data to be written is specified in the request data field. Data is packed as two
bytes per register. The normal response returns the function code, starting address, and quantity
of registers written.
• Use 0x03 standard function code to read Holding Registers
This function code is used to read the contents of a contiguous block of holding registers in
the Control Unit. The Request PDU specifies the starting register address and the number of
registers. In the PDU registers are addressed starting at zero. Therefore registers numbered 1-16
are addressed as 0-15. The register data in the response message are packed as two bytes per
register, with the binary contents right justified within each byte.
12.1.2.7.2.2 Incyte Holding Registers Table
Table 8: Incyte holding registers table.
12.1.2.7.2.3 Dencytee Holding Registers Table
Channel 1				
Mark Zero auto	 33		 word 16bits	 1
Clear Zero auto	 34		 word 16bits	 1
Recepy number Unit Factor	 35		 word 16bits	 1
Recepy number Measure Mode	 36		 word 16bits	 1
Reserved	 37		 word 16bits	 1
Reserved	 38		 word 16bits	 1
Reserved	 39		 word 16bits	 1
Reserved	 40		 word 16bits	 1
Offset zero	 41	 pF/cm	 Float 32bits	 2
Command Val. Dencytee	 43		 word 16bits	 1
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
127Cell Density Monitoring System Operating Instructions
Parameter Val. Dencytee	 44		 word 16bits	 1
Command Cal. Dencytee	 45		 word 16bits	 1
Parameter Cal. Dencytee	 46		 word 16bits	 1
Reserved	 47		 word 16bits	 1
Reserved	 48		 word 16bits	 1
Channel 2				
Mark Zero auto	 49		 word 16bits	 1
Clear Zero auto	 50		 word 16bits	 1
Recepy number Unit Factor	 51		 word 16bits	 1
Recepy number Measure Mode	 52		 word 16bits	 1
Reserved	 53		 word 16bits	 1
Reserved	 54		 word 16bits	 1
Reserved	 55		 word 16bits	 1
Reserved	 56		 word 16bits	 1
Offset zero	 57	 pF/cm	 Float 32bits	 2
Command Val. Dencytee	 59		 word 16bits	 1
Parameter Val. Dencytee	 60		 word 16bits	 1
Command Cal. Dencytee	 61		 word 16bits	 1
Parameter Cal. Dencytee	 62		 word 16bits	 1
Reserved	 63		 word 16bits	 1
Reserved	 64		 word 16bits	 1
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
Table 9: Dencytee holding registers table.
12.1.2.7.2.4 Allowed Values for Holding Registers
Only the following values may be written in the holding registers:
Register Name	 Value(s)
Mark Zero	 1
Clear Zero	 1
Activate Auto Cleaning	 0 to 1
Recipe Number Unit Factor	 0 to 8
Recipe Number Measure Mode	 0 to 3
Recipe Number Cleaning	 0 to 1
Table 10: Allowed values for holding registers.
12.1.2.8 Modbus Tables Arc View Controller 465 (XL)
12.1.2.8.1 Input Registers Tables (Read only)
12.1.2.8.1.1 Read the Modbus Commands
All measurements and readable information which comes from the sensors are memorized in
the Modbus input registers table. The Modbus function code 0x04 is used to read this table.
It allows reading 1 to 125 contiguous input registers from the Arc View Controller. The request
specifies the starting address and the number of registers to be read. In the Modbus frame,
registers are addressed starting at zero. For example, input registers numbered 1-16 are addressed
as 0-15 inside the frame.
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
129Cell Density Monitoring System Operating Instructions
Gateway serial number 	 001	  	 string	 6
Measures - Ch 1	  	  	  	  
Sensor serial number	 007	  	 string	 6
Amplifier serial number	 013	  	 string	 6
Amplifier status	 019	  	 word 16bits	 1
Conductivity 	 020	 mS/cm	 float32	 2
Permittivity	 022	 pF/cm	float32	 2
Measure Index	 024	  	 unsigned long	 2
Amplifier Status - float format	 026	  	 float32	 2
Reserved	 028		 float32	 2
Reserved	 030		 float32	 2
Reserved	 032		 float32	 2
Reserved	 034		 float32	 2
Reserved	 036		 float32	 2
Acknowledge Val. Incyte	 038	  	 word 16bits	 1
State Val. Incyte	 039	  	 word 16bits	 1
Acknowledge Cal. Incyte	 040	  	 word 16bits	 1
State Cal. Incyte	 041	  	 word 16bits	 1
Spectro - Ch 1	  	  	  	  
DeltaEpsilon	 042	 	 float32	 2
Fc	 044	 	 float32	 2
Alpha	 046	 	 float32	 2
FscanEpsilon	 048	 	 float32	 2
Spectro index	 050	  	 unsigned long	 2
Reserved	 052		 Word 16bits	 1
Measures - Ch 2	  	  	  	  
Sensor serial number	 053	  	 string	 6
Amplifier serial number	 059	  	 string	 6
Amplifier status	 065	  	 word 16bits	 1
Conductivity 	 066	 mS/cm	 float32	 2
Permittivity	 068	 pF/cm	float32	 2
Measure Index	 070	  	 unsigned long	 2
Amplifier Status - float format	 072	  	 float32	 2
Reserved	 074		 float32	 2
Reserved	 076		 float32	 2
Reserved	 078		 float32	 2
Reserved	 080		 float32	 2
Reserved	 082		 float32	 2
Acknowledge Val. Incyte	 084	  	 word 16bits	 1
State Val. Incyte	 085	  	 word 16bits	 1
Acknowledge Cal. Incyte	 086	  	 word 16bits	 1
State Cal. Incyte	 087	  	 word 16bits	 1
Spectro - Ch 2	  	  	  	  
DeltaEpsilon	 088	 	 float32	 2
Fc	 090	 	 float32	 2
Alpha	 092	 	 float32	 2
FscanEpsilon	 094	 	 float32	 2
Spectro index	 096	  	 float32 / ul	 2
Reserved	 098	  	 word 16bits	 1
Measures - Ch 3	  	  	  	  
Sensor serial number	 099	  	 string	 6
Amplifier serial number	 105	  	 string	 6
Amplifier status	 111	  	 word 16bits	 1
Conductivity 	 112	 mS/cm	 float32	 2
Permittivity	 114	 pF/cm	float32	 2
Measure Index	 116	  	 unsigned long	 2
Amplifier Status - float format	 118	  	 float32	 2
Reserved	 120		 float32	 2
Reserved	 122		 float32	 2
Reserved	 124		 float32	 2
Reserved	 126		 float32	 2
Reserved	 128		 float32	 2
Acknowledge Val. Incyte	 130	  	 word 16bits	 1
State Val. Incyte	 131	  	 word 16bits	 1
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
12.1.2.8.1.2 Incyte Input Registers Table
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
131Cell Density Monitoring System Operating Instructions
Acknowledge Cal. Incyte	 132	  	 word 16bits	 1
State Cal. Incyte	 133	  	 word 16bits	 1
Spectro - Ch 3		  	  	  
DeltaEpsilon	 134	 	 float32	 2
Fc	 136	 	 float32	 2
Alpha	 138	 	 float32	 2
FscanEpsilon	 140	 	 float32	 2
Spectro index	 142	  	 unsigned long	 2
Reserved	 144		 Word 16bits	 1
Measures - Ch 4	  	  	  	  
Sensor serial number	 145	  	 string	 6
Amplifier serial number	 151	  	 string	 6
Amplifier status	 157	  	 word 16bits	 1
Conductivity 	 158	 mS/cm	 float32	 2
Permittivity	 160	 pF/cm	float32	 2
Measure Index	 162	  	 unsigned long	 2
Amplifier Status - float format	 164	  	 float32	 2
Reserved	 166		 float32	 2
Reserved	 168		 float32	 2
Reserved	 170		 float32	 2
Reserved	 172		 float32	 2
Reserved	 174		 float32	 2
Acknowledge Val. Incyte	 176	  	 word 16bits	 1
State Val. Incyte	 177	  	 word 16bits	 1
Acknowledge Cal. Incyte	 178	  	 word 16bits	 1
State Cal. Incyte	 179	  	 word 16bits	 1
Spectro - Ch 4		  	  	  
DeltaEpsilon	 180	 	 float32	 2
Fc	 182	 	 float32	 2
Alpha	 184	 	 float32	 2
FscanEpsilon	 186	 	 float32	 2
Spectro index	 188	  	 unsigned long	 2
Reserved	 190		 Word 16bits	 1
Info-Fscan-Ch1	  	  	  	  
Reserved	 191	  	 word 16bits	 1
Reserved	 192	  	 word 16bits	 1
Reserved	 193	  	 word 16bits	 1
index-Fscan-ch1	 194	  	 unsigned long	 2
Freq-FSCAN-Ch1(30 points)		  	  	  
Scan-Freq-Pt0	 196	 Hz	 float 32 bits	 2
Scan-Freq-Pt1	 198	 Hz	 float 32 bits	 2
Scan-Freq-Pt2	 200	 Hz	 float 32 bits	 2
Scan-Freq-Pt3	 202	 Hz	 float 32 bits	 2
Scan-Freq-Pt4	 204	 Hz	 float 32 bits	 2
Scan-Freq-Pt5	 206	 Hz	 float 32 bits	 2
Scan-Freq-Pt6	 208	 Hz	 float 32 bits	 2
Scan-Freq-Pt7	 210	 Hz	 float 32 bits	 2
Scan-Freq-Pt8	 212	 Hz	 float 32 bits	 2
Scan-Freq-Pt9	 214	 Hz	 float 32 bits	 2
Scan-Freq-Pt10	 216	 Hz	 float 32 bits	 2
Scan-Freq-Pt11	 218	 Hz	 float 32 bits	 2
Scan-Freq-Pt12	 220	 Hz	 float 32 bits	 2
Scan-Freq-Pt13	 222	 Hz	 float 32 bits	 2
Scan-Freq-Pt14	 224	 Hz	 float 32 bits	 2
Scan-Freq-Pt15	 226	 Hz	 float 32 bits	 2
Scan-Freq-Pt16	 228	 Hz	 float 32 bits	 2
Scan-Freq-Pt17	 230	 Hz	 float 32 bits	 2
Scan-Freq-Pt18	 232	 Hz	 float 32 bits	 2
Scan-Freq-Pt19	 234	 Hz	 float 32 bits	 2
Scan-Freq-Pt20	 236	 Hz	 float 32 bits	 2
Scan-Freq-Pt21	 238	 Hz	 float 32 bits	 2
Scan-Freq-Pt22	 240	 Hz	 float 32 bits	 2
Scan-Freq-Pt23	 242	 Hz	 float 32 bits	 2
Scan-Freq-Pt24	 244	 Hz	 float 32 bits	 2
Scan-Freq-Pt25	 246	 Hz	 float 32 bits	 2
Scan-Freq-Pt26	 248	 Hz	 float 32 bits	 2
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
133Cell Density Monitoring System Operating Instructions
Scan-Freq-Pt27	 250	 Hz	 float 32 bits	 2
Scan-Freq-Pt28	 252	 Hz	 float 32 bits	 2
Scan-Freq-Pt29	 254	 Hz	 float 32 bits	 2
C(f)-FSCAN-Ch1(30 points)		  	  	  
Scan-C(f)-Pt0	 256	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt1	 258	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt2	 260	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt3	 262	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt4	 264	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt5	 266	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt6	 268	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt7	 270	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt8	 272	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt9	 274	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt10	 276	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt11	 278	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt12	 280	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt13	 282	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt14	 284	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt15	 286	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt16	 288	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt17	 290	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt18	 292	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt19	 294	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt20	 296	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt21	 298	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt22	 300	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt23	 302	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt24	 304	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt25	 306	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt26	 308	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt27	 310	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt28	 312	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt29	 314	 pF/cm	 float 32 bits	 2
Info-Fscan-Ch2		  	  	
Reserved	 316	  	 word 16bits	 1
Reserved	 317	  	 word 16bits	 1
Reserved	 318	  	 word 16bits	 1
index-Fscan-ch2	 319	  	 unsigned long	 2
Freq-FSCAN-Ch2(30 points)		  	  	  
Scan-Freq-Pt0	 321	 Hz	 float 32 bits	 2
Scan-Freq-Pt1	 323	 Hz	 float 32 bits	 2
Scan-Freq-Pt2	 325	 Hz	 float 32 bits	 2
Scan-Freq-Pt3	 327	 Hz	 float 32 bits	 2
Scan-Freq-Pt4	 329	 Hz	 float 32 bits	 2
Scan-Freq-Pt5	 331	 Hz	 float 32 bits	 2
Scan-Freq-Pt6	 333	 Hz	 float 32 bits	 2
Scan-Freq-Pt7	 335	 Hz	 float 32 bits	 2
Scan-Freq-Pt8	 337	 Hz	 float 32 bits	 2
Scan-Freq-Pt9	 339	 Hz	 float 32 bits	 2
Scan-Freq-Pt10	 341	 Hz	 float 32 bits	 2
Scan-Freq-Pt11	 343	 Hz	 float 32 bits	 2
Scan-Freq-Pt12	 345	 Hz	 float 32 bits	 2
Scan-Freq-Pt13	 347	 Hz	 float 32 bits	 2
Scan-Freq-Pt14	 349	 Hz	 float 32 bits	 2
Scan-Freq-Pt15	 351	 Hz	 float 32 bits	 2
Scan-Freq-Pt16	 353	 Hz	 float 32 bits	 2
Scan-Freq-Pt17	 355	 Hz	 float 32 bits	 2
Scan-Freq-Pt18	 357	 Hz	 float 32 bits	 2
Scan-Freq-Pt19	 359	 Hz	 float 32 bits	 2
Scan-Freq-Pt20	 361	 Hz	 float 32 bits	 2
Scan-Freq-Pt21	 363	 Hz	 float 32 bits	 2
Scan-Freq-Pt22	 365	 Hz	 float 32 bits	 2
Scan-Freq-Pt23	 367	 Hz	 float 32 bits	 2
Scan-Freq-Pt24	 369	 Hz	 float 32 bits	 2
Scan-Freq-Pt25	 371	 Hz	 float 32 bits	 2
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
135Cell Density Monitoring System Operating Instructions
Scan-Freq-Pt26	 373	 Hz	 float 32 bits	 2
Scan-Freq-Pt27	 375	 Hz	 float 32 bits	 2
Scan-Freq-Pt28	 377	 Hz	 float 32 bits	 2
Scan-Freq-Pt29	 379	 Hz	 float 32 bits	 2
C(f)-FSCAN-Ch2(30 points)		  	  	  
Scan-C(f)-Pt0	 381	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt1	 383	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt2	 385	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt3	 387	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt4	 389	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt5	 391	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt6	 393	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt7	 395	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt8	 397	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt9	 399	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt10	 401	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt11	 403	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt12	 405	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt13	 407	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt14	 409	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt15	 411	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt16	 413	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt17	 415	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt18	 417	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt19	 419	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt20	 421	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt21	 423	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt22	 425	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt23	 427	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt24	 429	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt25	 431	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt26	 433	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt27	 435	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt28	 437	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt29	 439	 pF/cm	 float 32 bits	 2
Info-Fscan-Ch3		  	  	  
Reserved	 441	  	 word 16bits	 1
Reserved	 442	  	 word 16bits	 1
Reserved	 443	  	 word 16bits	 1
index-Fscan-ch1	 444	  	 unsigned long	 2
Freq-FSCAN-Ch3(30 points)		  	  	  
Scan-Freq-Pt0	 446	 Hz	 float 32 bits	 2
Scan-Freq-Pt1	 448	 Hz	 float 32 bits	 2
Scan-Freq-Pt2	 450	 Hz	 float 32 bits	 2
Scan-Freq-Pt3	 452	 Hz	 float 32 bits	 2
Scan-Freq-Pt4	 454	 Hz	 float 32 bits	 2
Scan-Freq-Pt5	 456	 Hz	 float 32 bits	 2
Scan-Freq-Pt6	 458	 Hz	 float 32 bits	 2
Scan-Freq-Pt7	 460	 Hz	 float 32 bits	 2
Scan-Freq-Pt8	 462	 Hz	 float 32 bits	 2
Scan-Freq-Pt9	 464	 Hz	 float 32 bits	 2
Scan-Freq-Pt10	 466	 Hz	 float 32 bits	 2
Scan-Freq-Pt11	 468	 Hz	 float 32 bits	 2
Scan-Freq-Pt12	 470	 Hz	 float 32 bits	 2
Scan-Freq-Pt13	 472	 Hz	 float 32 bits	 2
Scan-Freq-Pt14	 474	 Hz	 float 32 bits	 2
Scan-Freq-Pt15	 476	 Hz	 float 32 bits	 2
Scan-Freq-Pt16	 478	 Hz	 float 32 bits	 2
Scan-Freq-Pt17	 480	 Hz	 float 32 bits	 2
Scan-Freq-Pt18	 482	 Hz	 float 32 bits	 2
Scan-Freq-Pt19	 484	 Hz	 float 32 bits	 2
Scan-Freq-Pt20	 486	 Hz	 float 32 bits	 2
Scan-Freq-Pt21	 488	 Hz	 float 32 bits	 2
Scan-Freq-Pt22	 490	 Hz	 float 32 bits	 2
Scan-Freq-Pt23	 492	 Hz	 float 32 bits	 2
Scan-Freq-Pt24	 494	 Hz	 float 32 bits	 2
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
137Cell Density Monitoring System Operating Instructions
Scan-Freq-Pt25	 496	 Hz	 float 32 bits	 2
Scan-Freq-Pt26	 498	 Hz	 float 32 bits	 2
Scan-Freq-Pt27	 500	 Hz	 float 32 bits	 2
Scan-Freq-Pt28	 502	 Hz	 float 32 bits	 2
Scan-Freq-Pt29	 504	 Hz	 float 32 bits	 2
C(f)-FSCAN-Ch3(30 points)		  	  	  
Scan-C(f)-Pt0	 506	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt1	 508	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt2	 510	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt3	 512	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt4	 514	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt5	 516	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt6	 518	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt7	 520	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt8	 522	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt9	 524	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt10	 526	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt11	 528	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt12	 530	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt13	 532	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt14	 534	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt15	 536	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt16	 538	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt17	 540	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt18	 542	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt19	 544	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt20	 546	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt21	 548	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt22	 550	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt23	 552	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt24	 554	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt25	 556	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt26	 558	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt27	 560	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt28	 562	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt29	 564	 pF/cm	 float 32 bits	 2
Info-Fscan-Ch4		  	  	
Reserved	 566	  	 word 16bits	 1
Reserved	 567	  	 word 16bits	 1
Reserved	 568	  	 word 16bits	 1
index-Fscan-ch2	 569	  	 unsigned long	 2
Freq-FSCAN-Ch4(30 points)		  	  	  
Scan-Freq-Pt0	 571	 Hz	 float 32 bits	 2
Scan-Freq-Pt1	 573	 Hz	 float 32 bits	 2
Scan-Freq-Pt2	 575	 Hz	 float 32 bits	 2
Scan-Freq-Pt3	 577	 Hz	 float 32 bits	 2
Scan-Freq-Pt4	 579	 Hz	 float 32 bits	 2
Scan-Freq-Pt5	 581	 Hz	 float 32 bits	 2
Scan-Freq-Pt6	 583	 Hz	 float 32 bits	 2
Scan-Freq-Pt7	 585	 Hz	 float 32 bits	 2
Scan-Freq-Pt8	 587	 Hz	 float 32 bits	 2
Scan-Freq-Pt9	 589	 Hz	 float 32 bits	 2
Scan-Freq-Pt10	 591	 Hz	 float 32 bits	 2
Scan-Freq-Pt11	 593	 Hz	 float 32 bits	 2
Scan-Freq-Pt12	 595	 Hz	 float 32 bits	 2
Scan-Freq-Pt13	 597	 Hz	 float 32 bits	 2
Scan-Freq-Pt14	 599	 Hz	 float 32 bits	 2
Scan-Freq-Pt15	 601	 Hz	 float 32 bits	 2
Scan-Freq-Pt16	 603	 Hz	 float 32 bits	 2
Scan-Freq-Pt17	 605	 Hz	 float 32 bits	 2
Scan-Freq-Pt18	 607	 Hz	 float 32 bits	 2
Scan-Freq-Pt19	 609	 Hz	 float 32 bits	 2
Scan-Freq-Pt20	 611	 Hz	 float 32 bits	 2
Scan-Freq-Pt21	 613	 Hz	 float 32 bits	 2
Scan-Freq-Pt22	 615	 Hz	 float 32 bits	 2
Scan-Freq-Pt23	 617	 Hz	 float 32 bits	 2
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
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139Cell Density Monitoring System Operating Instructions
Scan-Freq-Pt24	 619	 Hz	 float 32 bits	 2
Scan-Freq-Pt25	 621	 Hz	 float 32 bits	 2
Scan-Freq-Pt26	 623	 Hz	 float 32 bits	 2
Scan-Freq-Pt27	 625	 Hz	 float 32 bits	 2
Scan-Freq-Pt28	 627	 Hz	 float 32 bits	 2
Scan-Freq-Pt29	 629	 Hz	 float 32 bits	 2
C(f)-FSCAN-Ch4(30 points)		  	  	  
Scan-C(f)-Pt0	 631	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt1	 633	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt2	 635	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt3	 637	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt4	 639	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt5	 641	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt6	 643	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt7	 645	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt8	 647	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt9	 649	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt10	 651	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt11	 653	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt12	 655	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt13	 657	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt14	 659	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt15	 661	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt16	 663	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt17	 665	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt18	 667	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt19	 669	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt20	 671	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt21	 673	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt22	 675	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt23	 677	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt24	 679	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt25	 681	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt26	 683	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt27	 685	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt28	 687	 pF/cm	 float 32 bits	 2
Scan-C(f)-Pt29	 689	 pF/cm	 float 32 bits	 2
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
Table 11: Incyte Input Registers.
12.1.2.8.1.3 Dencytee Input Registers Table
Gateway serial number 	 693	  	 string	 6
Measures - Ch 1		  	  	  
Sensor serial number	 699	  	 string	 6
Amplifier serial number	 705		 string	 6
Amplifier status	 711		 word 16 bits	 1
Reserved	 712		 float 32	 2
Biomass	 714		 float 32	 2
Measure Index	 716		 unsigned long	 2
Amplifier Status – float format	 718		 float 32	 2
Reserved	 720		 float 32	 2
Reserved	 722		 float 32	 2
Reserved	 724		 float 32	 2
Reserved	 726		 float 32	 2
Reserved	 728		 float 32	 2
Acknowledge Val. Dencytee	 730		 word 16 bits	 1
State Val. Dencytee	 731		 word 16 bits	 1
Acknowledge Cal. Dencytee	 732		 word 16 bits	 1
State Cal. Dencytee	 733		 word 16 bits	 1
Reserved	 734		 float32	 2
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
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141Cell Density Monitoring System Operating Instructions
Reserved	 736		 float 32	 2
Reserved	 738		 float 32	 2
Reserved	 740		 float 32	 2
Reserved	 742		 float 32	 2
Reserved	 744		 float 32	 2
Measures - Ch 2		  	  	  
Sensor serial number	 746	  	 string	 6
Amplifier serial number	 752		 string	 6
Amplifier status	 758		 word 16 bits	 1
Reserved	 759		 float 32	 2
Biomass	 761		 float 32	 2
Measure Index	 763		 unsigned long	 2
Amplifier Status – float format	 765		 float 32	 2
Reserved	 767		 float 32	 2
Reserved	 769		 float 32	 2
Reserved	 771		 float 32	 2
Reserved	 773		 float 32	 2
Reserved	 775		 float 32	 2
Acknowledge Val. Dencytee	 777		 word 16 bits	 1
State Val. Dencytee	 778		 word 16 bits	 1
Acknowledge Cal. Dencytee	 779		 word 16 bits	 1
State Cal. Dencytee	 780		 word 16 bits	 1
Reserved	 781		 float32	 2
Reserved	 783		 float 32	 2
Reserved	 785		 float 32	 2
Reserved	 787		 float 32	 2
Reserved	 789		 float 32	 2
Reserved	 791		 float 32	 2
Measures - Ch 3		  	  	  
Sensor serial number	 793	  	 string	 6
Amplifier serial number	 799		 string	 6
Amplifier status	 805		 word 16 bits	 1
Reserved	 806		 float 32	 2
Biomass	 808		 float 32	 2
Measure Index	 810		 unsigned long	 2
Amplifier Status – float format	 812		 float 32	 2
Reserved	 814		 float 32	 2
Reserved	 816		 float 32	 2
Reserved	 818		 float 32	 2
Reserved	 820		 float 32	 2
Reserved	 822		 float 32	 2
Acknowledge Val. Dencytee	 824		 word 16 bits	 1
State Val. Dencytee	 825		 word 16 bits	 1
Acknowledge Cal. Dencytee	 826		 word 16 bits	 1
State Cal. Dencytee	 827		 word 16 bits	 1
Reserved	 828		 float32	 2
Reserved	 830		 float 32	 2
Reserved	 832		 float 32	 2
Reserved	 834		 float 32	 2
Reserved	 836		 float 32	 2
Reserved	 838		 float 32	 2
Measures - Ch 4		  	  	  
Sensor serial number	 840	  	 string	 6
Amplifier serial number	 846		 string	 6
Amplifier status	 852		 word 16 bits	 1
Reserved	 853		 float 32	 2
Biomass	 855		 float 32	 2
Measure Index	 857		 unsigned long	 2
Amplifier Status – float format	 859		 float 32	 2
Reserved	 861		 float 32	 2
Reserved	 863		 float 32	 2
Reserved	 865		 float 32	 2
Reserved	 867		 float 32	 2
Reserved	 869		 float 32	 2
Acknowledge Val. Dencytee	 871		 word 16 bits	 1
State Val. Dencytee	 872		 word 16 bits	 1
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
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143Cell Density Monitoring System Operating Instructions
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
Acknowledge Cal. Dencytee	 873		 word 16 bits	 1
State Cal. Dencytee	 874		 word 16 bits	 1
Reserved	 875		 float32	 2
Reserved	 877		 float 32	 2
Reserved	 879		 float 32	 2
Reserved	 881		 float 32	 2
Reserved	 883		 float 32	 2
Reserved	 885		 float 32	 2
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
Table 12: Dencytee input register.
12.1.2.8.2 Holding Registers (read and write)
12.1.2.8.2.1 Read and Write Function Code
Writing holding registers allow changing configuration and performs several actions.
• Use 0x06 standard function code to write single holding register.
This function code is used to write a single holding register in the Arc View Controller. The Request
PDU specifies the address of the register to be written. Registers are addressed starting
at zero. Therefore register numbered 1 is addressed as 0. The normal response is an echo of
the request, returned after the register contents have been written.
• Use 0x10 standard function code to write holding registers.
This function code is used to write a block of contiguous registers (1 to 123 registers) in the Arc
View Controller. The data to be written is specified in the request data field. Data is packed as
two bytes per register. The normal response returns the function code, starting address, and
quantity of registers written.
• Use 0x03 standard function code to read Holding Registers
This function code is used to read the contents of a contiguous block of holding registers in the
Arc View Controller. The Request PDU specifies the starting register address and the number of
registers. In the PDU registers are addressed starting at zero. Therefore registers numbered 1-16
are addressed as 0-15. The register data in the response message are packed as two bytes per
register, with the binary contents right justified within each byte.
12.1.2.8.2.2 Incyte Holding Registers Table
Channel 1				
Mark Zero auto	 1		 word 16bits	 1
Clear Zero auto	 2		 word 16bits	 1
Recepy number Unit Factor	 3		 word 16bits	 1
Recepy number Measure Mode	 4		 word 16bits	 1
Activate Manual Cleaning	 5		 word 16bits	 1
Activate auto Cleaning	 6		 word 16bits	 1
Recepy number Cleaning	 7		 word 16bits	 1
Reserved	 8		 word 16bits	 1
Offset zero	 9	 pF/cm	 Float 32bits	 2
Command Val. Incyte	 11		 word 16bits	 1
Parameter Val. Incyte	 12		 word 16bits	 1
Command Cal. Incyte	 13		 word 16bits	 1
Parameter Cal. Incyte	 14		 word 16bits	 1
Reserved	 15		 word 16bits	 1
Reserved	 16		 word 16bits	 1
Channel 2				
Mark Zero auto	 17		 word 16bits	 1
Clear Zero auto	 18		 word 16bits	 1
Recepy number Unit Factor	 19		 word 16bits	 1
Recepy number Measure Mode	 20		 word 16bits	 1
Activate Manual Cleaning	 21		 word 16bits	 1
Activate auto Cleaning	 22		 word 16bits	 1
Recepy number Cleaning	 23		 word 16bits	 1
Reserved	 24		 word 16bits	 1
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145Cell Density Monitoring System Operating Instructions
Offset zero	 25	 pF/cm	 Float 32bits	 2
Command Val. Incyte	 27		 word 16bits	 1
Parameter Val. Incyte	 28		 word 16bits	 1
Command Cal. Incyte	 29		 word 16bits	 1
Parameter Cal. Incyte	 30		 word 16bits	 1
Reserved	 31		 word 16bits	 1
Reserved	 32		 word 16bits	 1
Channel 3				
Mark Zero auto	 33		 word 16bits	 1
Clear Zero auto	 34		 word 16bits	 1
Recepy numbrer Unit Factor	 35		 word 16bits	 1
Recepy number Measure Mode	 36		 word 16bits	 1
Activate Manual Cleaning	 37		 word 16bits	 1
Activate auto Cleaning	 38		 word 16bits	 1
Recepy number Cleaning	 39		 word 16bits	 1
Reserved	 40		 word 16bits	 1
Offset zero	 41	 pF/cm	 Float 32bits	 2
Command Val. Incyte	 43		 word 16bits	 1
Parameter Val. Incyte	 44		 word 16bits	 1
Command Cal. Incyte	 45		 word 16bits	 1
Parameter Cal. Incyte	 46		 word 16bits	 1
Reserved	 47		 word 16bits	 1
Reserved	 48		 word 16bits	 1
Channel 4				
Mark Zero auto	 49		 word 16bits	 1
Clear Zero auto	 50		 word 16bits	 1
Recepy numbrer Unit Factor	 51		 word 16bits	 1
Recepy number Measure Mode	 52		 word 16bits	 1
Activate Manual Cleaning	 53		 word 16bits	 1
Activate auto Cleaning	 54		 word 16bits	 1
Recepy number Cleaning	 55		 word 16bits	 1
Reserved	 56		 word 16bits	 1
Offset zero	 57	 pF/cm	 Float 32bits	 2
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
Table 13: Incyte holding registers table.
12.1.2.8.2.3 Dencytee Holding Registers Table
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
Channel 1				
Mark Zero auto	 71		 word 16bits	 1
Clear Zero auto	 72		 word 16bits	 1
Recepy number Unit Factor	 73		 word 16bits	 1
Recepy number Measure Mode	 74		 word 16bits	 1
Reserved	 75		 word 16bits	 1
Reserved	 76		 word 16bits	 1
Reserved	 77		 word 16bits	 1
Reserved	 78		 word 16bits	 1
Offset zero	 79	 pF/cm	 Float 32bits	 2
Command Val. Dencytee	 81		 word 16bits	 1
Parameter Val. Dencytee	 82		 word 16bits	 1
Command Cal. Dencytee	 83		 word 16bits	 1
Parameter Cal. Dencytee	 84		 word 16bits	 1
reserved	 85		 word 16bits	 1
reserved	 86		 word 16bits	 1
Channel 2				
Mark Zero auto	 87		 word 16bits	 1
Command Val. Incyte	 59		 word 16bits	 1
Parameter Val. Incyte	 60		 word 16bits	 1
Command Cal. Incyte	 61		 word 16bits	 1
Parameter Cal. Incyte	 62		 word 16bits	 1
Reserved	 63		 word 16bits	 1
Reserved	 64		 word 16bits	 1
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
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147Cell Density Monitoring System Operating Instructions
Clear Zero auto	 88		 word 16bits	 1
Recepy number Unit Factor	 89		 word 16bits	 1
Recepy number Measure Mode	 90		 word 16bits	 1
Reserved	 91		 word 16bits	 1
Reserved	 92		 word 16bits	 1
Reserved	 93		 word 16bits	 1
Reserved	 94		 word 16bits	 1
Offset zero	 95	 pF/cm	 Float 32bits	 2
Command Val. Dencytee	 97		 word 16bits	 1
Parameter Val. Dencytee	 98		 word 16bits	 1
Command Cal. Dencytee	 99		 word 16bits	 1
Parameter Cal. Dencytee	 100		 word 16bits	 1
Reserved	 101		 word 16bits	 1
Reserved	 102		 word 16bits	 1
Channel 3				
Mark Zero auto	 103		 word 16bits	 1
Clear Zero auto	 104		 word 16bits	 1
Recepy number Unit Factor	 105		 word 16bits	 1
Recepy number Measure Mode	 106		 word 16bits	 1
Reserved	 107		 word 16bits	 1
Reserved	 108		 word 16bits	 1
Reserved	 109		 word 16bits	 1
Reserved	 110		 word 16bits	 1
Offset zero	 111	 pF/cm	 Float 32bits	 2
Command Val. Dencytee	 113		 word 16bits	 1
Parameter Val. Dencytee	 114		 word 16bits	 1
Command Cal. Dencytee	 115		 word 16bits	 1
Parameter Cal. Dencytee	 116		 word 16bits	 1
Reserved	 117		 word 16bits	 1
Reserved	 118		 word 16bits	 1
Channel 4				
Mark Zero auto	 119		 word 16bits	 1
Clear Zero auto	 120		 word 16bits	 1
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
Table 14: Dencytee holding registers table.
12.1.2.8.2.4 Allowed Values for Holding Registers
Only the following values may be written in the holding registers:
Register Name	 Value(s)
Mark Zero	 1
Clear Zero	 1
Activate Auto Cleaning	 0 to 1
Recipe Number Unit Factor	 0 to 8
Recipe Number Measure Mode	 0 to 3
Recipe Number Cleaning	 0 to 1
Table 15: Allowed values for holding registers.
Recepy number Unit Factor	 121		 word 16bits	 1
Recepy number Measure Mode	 122		 word 16bits	 1
Reserved	 123		 word 16bits	 1
Reserved	 124		 word 16bits	 1
Reserved	 125		 word 16bits	 1
Reserved	 126		 word 16bits	 1
Offset zero	 127	 pF/cm	 Float 32bits	 2
Command Val. Dencytee	 129		 word 16bits	 1
Parameter Val. Dencytee	 130		 word 16bits	 1
Command Cal. Dencytee	 131		 word 16bits	 1
Parameter Cal. Dencytee	 132		 word 16bits	 1
Reserved	 133		 word 16bits	 1
Reserved	 134		 word 16bits	 1
Function	 Register	 Unit	 Data type	 Data Size
	 address			 (registers)
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149Cell Density Monitoring System Operating Instructions
12.2	 Connect by OPC Communication
NOTE: To connect the Arc View Controller or ComBox with the process control
system, the OPC License (Ref 243820) is needed. The Cell Density Integration Kit is
equipped with all licenses.
12.2.1	 Introduction to OPC
OPC is a standard which specifies the communication of real-time data between control devices
from different manufacturers. The Arc View Controller offers an OPC XML-DA server. OPC XMLDA
is the OPC Foundation’s adoption of the XML (eXtensible Markup Language) set of technologies.
An OPC XML-DA client is used to communicate with the Arc View Controller. The Arc
View Controller takes advantage of the “OPC-XML-DA-SDK-LIN” library supplied by Softing. The
library is OPC compliant.
12.2.2	 Configuration of the Control Unit
Enter the Administrator Settings (Figure 47), select the Network Applications and activate
OPC (Figure 62).
Figure 62: Activation of the OPC protocol.
The Arc View Controller OPC server provides the following data:
Arc View Controller:
•	 Controller name (with serial number): character string.
Sensor Unit:
•	 Amplifier serial number: character string.
•	 Sensor serial number: character string.
•	 Probe status: character string.
•	 Conductivity: floating point value.
•	 Permittivity: floating point value.
•	 Cell Density: floating point value.
•	 Fc (IncyteScan cutting frequency): floating point value.
•	 DeltaEps (IncyteScan Δε): floating point value.
•	 Alpha (IncyteScan α): floating point value.
•	 IncyteScanFrequencies: list of floating point values.
•	 C(f) (IncyteScan Permittivity values): list of floating point values.
•	 IncyteScanPermittivity (Permittivity measured by FScan method): floating point value.
NOTE: The refresh rate is about 30s for the Incyte Scan parameters and 3s for the
other parameters.
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151Cell Density Monitoring System Operating Instructions
12.2.3	 Configuration of the OPC Client
The connection of the OPC client to the Control Unit OPC server is made using the following
URL: http://ArcViewControllerName:8079/EVOOPC/XMLDA, where «ArcViewControllerName»
(ex: E265-XXX-XXX) is the name of the Control Unit that is on the sticker on the left side panel
and in the about menu (Figure 13) of the software. Alternatively, you can use the IP address.
NOTE: The Control Unit OPC server has been tested and validated with the OPC
Classic Demo client, from Softing and the OPC Viewer client from CommServer. Further
patches, to ensure the newest version of the bridge may be required.
12.2.4	 OPC Classic Demo Client Start Guide
12.2.4.1	 System Overview
Figure 63: OPC Classic Demo System Overview.
12.2.4.2	 Download and Installation
1.	 Download the OPC-Classic Demo installer for Windows from the Softing homepage. The
OPC client must be installed on a user PC connected to the local network.
2.	 After downloading OPC-Classic Demo, run the setup and follow the instructions.
3.	 Once the installation is completed, run the OPC Demo Client. If the installation was successful,
the following screen is displayed (Figure 64).
Figure 64: OPC Classic Demo client.
12.2.4.3	 Connecting the OPC Classic Demo Client to the Control Unit
1.	 Connect the Control Unit and the PC (with the OPC client) to the local network.
2.	 Connection of the OPC client on the PC to the integrated OPC server in the Control Unit
using the following URL: http://ArcViewControllerName:8079/EVOOPC/XMLDA, where
“ArcViewControllerName” (ex: E265-XXX-XXX) is the name of the Control Unit that is on
the sticker on the left side panel and in the About Menu (Figure 13) of the software. Alternatively,
the IP address of the device may be used. In the example below, the IP address
192.168.128.181 was used. Enter the OPC Server URL in the associated text control and
press add server (Figure 65).
CONTROL
UNIT
OPC CLIENT
LOCAL NETWORK
OPC XML-DA
USER PC
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153Cell Density Monitoring System Operating Instructions
Figure 65: Insertion of the Arc View Controller URL.
3.	 If the OPC Server URL is correct, the following screen (Figure 66) is displayed with a green
light beside the OPC server:
Figure 66: Connected Arc View Controller in the Classic Demo.
4.	 Select the DA Browse tab to do the data selection (Figure 67).
Figure 67: Data selection in the DA Browse tab.
5.	 Double click on each item that needs to be monitored. Each selected item is added to the list
(Figure 68).
Figure 68: Item selection in the DA Browse tab.
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155Cell Density Monitoring System Operating Instructions
6.	 To observe the data evolution select the DA Items tab to see the evolution of the data
selection (Figure 69).
Figure 69: Connected Arc View Controller with the Tool Box Demo Client.
ATTENTION! If there is a red light besides the items, activate the OPC server
on your Arc View Controller (see Chapter 9.4).
12.2.5	 Softing OPC Bridge Start Guide
12.2.5.1	 System Overview
Figure 70: System Overview of the Softing OPC bridge.
12.2.5.2	 Download and Installation
1.	 Purchase the dataFEED OPC Suite for Windows, available from Softing.
2. 	After downloading dataFEED OPC Suite, run the setup and follow the instructions.
3.	 Start the dataFEED OPC Suite Configurator on the Windows start menu
(Start => Programs => Softing OPC Easy Connect => dataFEED OPC Suite => Configurator).
4.	 Select the Tab Data Source on the left side and click on OPC Server (Figure 71).
CONTROL
UNIT
OPC BRIDGE
LOCAL NETWORK
OPC XML-DA
USER PC
OPC CLIENT
OPC DA
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157Cell Density Monitoring System Operating Instructions
Figure 71: Starting with the OPC Bridge.
5.	 Click on the add a new data source button. The OPC Server Wizard starts on the widget
(Figure 72).
6.	 Click next.
Figure 72: OPC Server Wizard.
7.	 Enter a name for the data source and select Server Type XML-DA (Figure 73).
Figure 73: Definition of the data source and the server type.
8.	 Connect the Control Unit to the local network. Connect the PC to the local network, the connection
of the OPC client to the Control Unit OPC server is made using the following URL:
http://ArcViewControllerName:8079/EVOOPC/XMLDA, where “ArcViewControllerName” (ex:
E265-XXX-XXX) is the name of the Control Unit that is on the sticker on the left side panel
and in the About Menu (Figure 13) in the Controller software. Alternatively, the IP address
of the device may be used. In the example below, the IP address 172.16.125.102 was used
(Figure 74). Enter the URL and proceed.
Figure 74: Definition of the
Control Unit URL.
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159Cell Density Monitoring System Operating Instructions
9.	 Test the connection (Figure 75).
Figure 75: Validating the server connection.
10. If the configuration was successful the following text (Figure 76) is written on the terminal.
Figure 76: Successful validation of the server connection.
11. Finish the valid bridge configuration without further adjustments (Figure 77).
Figure 77: ending the valid bridge configuration.
12.	Confirm the new settings to save them. If the configuration was successful, the screen
should look like the following (Figure 78).
Figure 78: Data Source Window of the OPC connection.
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161Cell Density Monitoring System Operating Instructions
13. Select Data Destination and choose OPC Client.
14. Copy the Class ID of the OPC Data Access (Figure 79).
Figure 79: Choose the OPC Client Data Destination.
15.	Start the OPC Bridge (local application => start). The settings are disabled and the
programm icon (upper left corner) is green (Figure 80).
Figure 80: Starting the OPC Bridge.
16. In order to test the bridge start the Softing OPC Classic Demo Client. Select the OPC Server
Tab and select the DA server (Figure 81).
Figure 81: Selection of the DA Server.
17. The Server is added to the left terminal and with an green status indication (Figure 82).
Figure 82: Activation of the DA Server.
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163Cell Density Monitoring System Operating Instructions
18.	Select the available DA items (types of information) in the tab DA Browse with right click and
the selection add item (Figure 83).
Figure 83: Selection of the DA Items.
19.	Display the selected DA Item values in the Tab DA Items (Figure 84).
Figure 84: Display of the selected DA Item values.
The OPC DA bridge is now functional and communicates with the OPC server of the Control
Unit. It may be connected with the favorite OPC DA client.
12.3	 Connecting the 4-20 mA Output Box
NOTE: Please be aware that the 4-20 mA Output Box (Ref 243850) is needed.
The Analog Output Box is specially designed to connect the Control Unit to the Process Control
Systems with 4-20 mA input cards. It has 4 outputs for cell density, 4 outputs for conductivity
(both 4-20 mA current sources) and 4 alarms (open drain). The Analog Output Box is designed
in a DIN rail for being installed in the cabinet. Refer to figure 61 to see the M12 Pin assignment.
12.3.1	 Electrical Installation
1.	 Connect the Arc 4-20 mA Output Box to the AUX M12 connector of the Control Unit (Figure 85).
VCD
TCD
ON/OFF
Channel 1 Channel 2
ModbusAUX
DC 24V
ModbusAUXChannel 2Channel 1
Figure 85: Connecting the 4-20mA Analog Box with the Arc View and ComBox.
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
165Cell Density Monitoring System Operating Instructions
2.	 Connect the outputs of the 4-20mA Output Box to the passive inputs of the process control
system (process control system is a current sink) (Figure 86, Figure 87, Figure 88).
•	 X (1/2/3/4) represents the cell density on Channel 1/2/3/4
•	 Sigma (1/2/3/4) represents the conductivity on Channel 1/2/3/4
•	 Alarm (1/2/3/4) represents the alarm of Channel 1/2/3/4
4-20 mA OUTPUTS
CONNECTIONS
ALARM OUTPUTS
CONNECTIONS
4-20 mA OUTPUTS
CONNECTIONS
ALARM OUTPUTS
CONNECTIONS
Figure 86: Port Configuration of the 4-20 Box.
+5V
X / Sigma
External load, max 150 Ω
4-20 mA Box
Figure 87: Schematic diagram of an analog output.
3.	 To use the alarm output, which is an open drain output, connect an external relay to the
Alarm output.
Vdd
Alarm X
4-20mA Box
imax = 1A
Figure 88: schematic diagram to connect an external relay to the Alarm output.
The channel and port overview is shown in table 17.
Channel	Pin	 Description	 Measurement
1	 1	 X1	 Cell Density Channel 1
	2		 Ground Cell Density Channel 1
	 3	 Sigma 1	 Conductivity Channel 1
	4		 Ground Condcutivity Channel 1
2	 1	 X2	 Cell Density Channel 2
	2		 Ground Cell Density Channel 2
	 3	 Sigma 2	 Conductivity Channel 2
	4		 Ground Condcutivity Channel 2
3	 1	 X3	 Cell Density Channel 3
	2		 Ground Cell Density Channel 3
	 3	 Sigma 3	 Conductivity Channel 3
	4		 Ground Condcutivity Channel 3	
4	 1	 X4	 Cell Density Channel 4
	2		 Ground Cell Density Channel 4
	 3	 Sigma 4	 Conductivity Channel 4
	4		 Ground Condcutivity Channel 4
Table 17: Channel and port overview.
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
167Cell Density Monitoring System Operating Instructions
12.3.2	 Software Configuration
1.	 Go to the Advanced settings (Figure 13) and select the 4-20 mA Configuration (Figure 85).
2.	 Select the Channel to configure the output range after 4-20 mA current output. Enter the cell
density measurement values for 4 mA and 20 mA output (Figure 89).
Figure 89: Software configuration of the 4-20 mA Box for Cell Density measurements.
3.	 Enter the conductivity measurement values for 4 mA and 20 mA output (Figure 90).
NOTE: Conductivity measurement is just available for Incyte Sensors.
Figure 90: Software Configuration of the 4-20 mA Box for conductivity.
4.	 Select a trigger for the alarm output. Choose from cell density, conductivity or measurement
status. and define the minimal and maximal threshold, where an alarm should be triggered
(Figure 91).
Figure 91: Set Up of the Alarms.
12.4	 Connect by Profibus Communication
12.4.1	 Profibus General Information
The Arc View Controller Modbus-Profibus DP Module is already pre-configured. The ID for the
Card must be set correctly. The GSD file provided must be filed to the process control system
for it to recognize the Module. The GSD file is available at: www.hamiltoncompany.com/
arc_view_update
ATTENTION! The Arc View Controller Modbus-Profibus DP Module is only
available for Arc View Controller 465 and Arc View Controller 465 XL.
NOTE: Please be aware that the Modbus-Profibus module(Ref 243889) and M12 /
open end cables (5 m, Ref 243851, 10 m, Ref 243852) are needed.
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
169Cell Density Monitoring System Operating Instructions
12.4.1.1	 Ground the Modbus-Profibus module
NOTE: If the terminal 3 is not connected, the Modbus is in a floating state. Normally
(with short cables)  this is not an issue but if EMI (EMC) issues are present it may cause 
a problem. IIn this case terminal 3 should be connected together with the PWR supply
GND to hold the bus line to a defined potential (Figure 92).
Modbus line
brown
yellow
Rx232
Tx232
AP-GND
Rx422+
Rx422-
Tx422+
Tx422-
RS485-Termination:
Rx422 = off
Tx422 = on
*)
1
2
3
4
5
6
7
RS485+
RS485I/O
1
I/O 3
I/O 5
I/O 7
I/O 2
I/O 4
I/O 6
I/O 8
X6
X1
Rx422*TerminationTx422*
1
3
5
7
2
4
6
8
Deutschmann
Automation
CL-Profibus
OnOffOnOff
Power supply
10...33 VDCX2
Tx-D
ebug
R
x-D
ebug
0V
(Pw
r)
U
B
(Pw
r)
4
3
2
1
Figure 92: Overview of the Modbus-Profibus module
12.4.2	 Profibus Interface
12.4.2.1	Overview
The following information is always available in the Profibus output buffer:
Incyte:
•	 Amplifier Status Incyte (word, 2 Bytes)
•	 Conductivity (float, 4 Bytes)
•	 Permittivity (float, 4 Bytes)
•	 Measure Index Incyte (unsigned long, 4 Bytes)
CONNECTION OF THE CONTROL UNIT
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CONNECTION OF THE CONTROL UNIT
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•	 Delta Epsilon (float, 4 Bytes)
•	 Fc (float, 4 Bytes)
•	 Alpha (float, 4 Bytes)
•	 FScanEpsilon (float, 4 Bytes)
•	 SpectroIndex (unsigned long, 4 Bytes)
Dencytee:
•	 Amplifier Status Dencytee (word, 2 Bytes)
•	 Biomass (float, 4 Bytes)
•	 Measure Index Dencytee (unsigned long, 4 Bytes)
12.4.2.2	 Input Buffer
The input buffer must be at least 8 byte and contain the function code and the sensor type (see
page 9, Function codes) for every channel:
Byte Number	 Type	 Content
0	 byte	 Sensor type Channel 1
1	 byte	 Function code Channel 1
2	 byte	 Sensor type Channel 2
3	 byte	 Function code Channel 2
4	 byte	 Sensor type Channel 3
5	 byte	 Function code Channel 3
6	 byte	 Sensor type Channel 4
7	 byte	 Function code Channel 4
Table 18: Input buffer table
12.4.2.3	 Output Buffer
The following table defines the content of the output buffer for each channel. The data is refreshed
continously and depends on the defined sensor type (defined in the input buffer). The
corresponding Modbus Registers and lengths in the Arc View Controller are defined in brackets.
For data requested by a function code one byte-array per channel is reserved in the output.
171Cell Density Monitoring System Operating Instructions
Byte	 Type	 Sensor type	 Sensor type	 Sensor type
Number		 Channel 1 = 0 (none)	 Channel 1 = 1 Incyte 	 Channel 1 = 2 Dencytee
0…1	 word 	 0x0000	 Amplifier Status Capa	 Amplifier Status OD
	 16bit		 (19/1)	 (711/1)
2…5	 float	 0.0f	 Conductivity (20/2)	 0.0f
6…9	 float	 0.0f	 Permittivity (22/2)	 Biomass (714/2)
10…13	 uint32	 0	 Measure Index Capa (24/2)	 Measure Index OD (716/2)
14…17	 float	 0.0f	 Delta Epsilon (42/2)	 0.0f
18…21	 float	 0.0f	 Fc (44/2)	 0.0f
22…25	 float	 0.0f	 Alpha (46/2)	 0.0f
26…29	 float	 0.0f	 FScanEpsilon (48/2)	 0.0f
30…33	 uint32	 0	 SpectroIndex (50/2)	 0
34…45	 byte[]	 0x00	 Data requested by the function code for the channel	
Byte	 Type	 Sensor type	 Sensor type	 Sensor type
Number		 Channel 2 = 0 (none)	 Channel 2 = 1 Incyte 	 Channel 2 = 2 Dencytee
46…47	 word	 0x0000	 Amplifier Status Capa	 Amplifier Status OD
	 16bit		 (65/1)	 (758/1)
48…51	 float	 0.0f	 Conductivity (66/2)	 0.0f
52…55	 float	 0.0f	 Permittivity (68/2)	 Biomass (761/2)
56…59	 uint32	 0	 Measure Index Capa	 Measure Index OD
			 (70/2)	 (763/2)
60…63	 float	 0.0f	 Delta Epsilon (88/2)	 0.0f
64…67	 float	 0.0f	 Fc (90/2)	 0.0f
68…71	 float	 0.0f	 Alpha (92/2)	 0.0f
72…75	 float	 0.0f	 FScanEpsilon (94/2)	 0.0f
76…79	 uint32	 0	 SpectroIndex (96/2)	 0
80…91	 byte[]	 0x00	 Data requested by the function code for the channel
Byte	 Type	 Sensor type	 Sensor type	 Sensor type
Number		 Channel 3 = 0 (none)	 Channel 3 = 1 Incyte 	 Channel 3 = 2 Dencytee
92…93	 word	 0x0000	 Amplifier Status Capa	 Amplifier Status OD
	 16bit		 (111/1)	 (805/1)
94…97	 float	 0.0f	 Conductivity (112/2)	 0.0f
98…101	 float	 0.0f	 Permittivity (114/2)	 Biomass (808/2)
102…105	 uint32	 0	 Measure Index Capa	 Measure Index OD
			 (116/2)	 (810/2)
106…109	 float	 0.0f	 Delta Epsilon (134/2)	 0.0f
110…113	 float	 0.0f	 Fc (136/2)	 0.0f
114…117	 float	 0.0f	 Alpha (138/2)	 0.0f
118…121	 float	 0.0f	 FScanEpsilon (140/2)	 0.0f
122…125	 uint32	 0	 SpectroIndex (142/2)	 0
126…137	 byte[]	 0x00	 Data requested by the function code for the channel
Byte	 Type	 Sensor type	 Sensor type	 Sensor type
Number		 Channel 4 = 0 (none)	 Channel 4 = 1 Incyte 	 Channel 4 = 2 Dencytee
138…139	 word	 0x0000	 Amplifier Status Capa	 Amplifier Status OD
	 16bit		 (157/1)	 (852/1)
140…143	 float	 0.0f	 Conductivity (158/2)	 0.0f
144…147	 float	 0.0f	 Permittivity (160/2)	 Biomass (855/2)
148…151	 uint32	 0	 Measure Index Capa	 Measure Index OD
			 (162/2)	 (857/2)
152…155	 float	 0.0f	 Delta Epsilon (180/2)	 0.0f
156…159	 float	 0.0f	 Fc (182/2)	 0.0f
160…163	 float	 0.0f	 Alpha (184/2)	 0.0f
164…167	 float	 0.0f	 FScanEpsilon (186/2)	 0.0f
168…171	 uint32	 0	 SpectroIndex (188/2)	 0
172…183	 byte[]	 0x00	 Data requested by the function code for the channel
Byte Number	 Type	 Content
184	 byte	Error
Table 19: Input buffer table
The output data is refreshed every second.
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CONNECTION OF THE CONTROL UNIT
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173Cell Density Monitoring System Operating Instructions
12.4.2.4	 Decoding the Return Values
Characters
According to the Modbus convention, the characters are interchanged byte-wise.
The character «01234567» will be transmitted as «10325476.»
Float, Integers
According to the Modbus convention, the 4 byte values are interchanged word/2-byte-wise.
For example: the float value 2.54 has the hex representation of 0x40228f5c. The Arc View
Controller puts this value as 0x8f5c 4022 on the line.
Bit Masks
When an interface error to the Arc View Controller occurs, the module makes a retry. If this
retry fails, the module puts an error on byte 184 and the all other fields of the output buffer are
set to 0x00.
Byte 184 	 Description
0x00	 Everything ok
0x01	 Arc View Controller replies with an error
	 (Arc View Controller replies correctly, but the answer contains an error code)
0x02	 Wrong answer
	 (no Arc View Controller available, Arc View Controller sends an invalid message
	 or no message)
Table 20: Return values of the Arc View Controller
The pre-amplifier status looks like this:
Hex Code	 Description
0x00	 SENSOR: not found
	 No sensor connected to the preamplifier.
0x01	 SENSOR: sterilization count overflow
	 The sensor has been sterilized more than 100 times. Please contact your local
	representative.
Hex Code	 Description
0x04	 AMPLIFIER : total duration of use overflow
	 The preamplifier has been used more than 1 year continuously. Please perform
	 a preamplifier check
0x05	 SENSOR: invalid sensor calibration
	 The current sensor has not been calibrated with the preamplifier
0x06	 SENSOR: invalid calibration
	 The current sensor has not been calibrated with the preamplifier. The sensor
	 serial number is not valid.
0x07	 SENSOR: total duration overflow
	 The sensor has been used more than 6 months continuously. Please perform
	 a probe check
0x08	 SENSOR: out of calibration range
	 The measurement is out of the calibration range.
0x09	 SENSOR: cleaning state
	 The preamplifier is applying specific voltage to clean the sensor, no
	 measurement available.
0x0a	 AMPLIFIER: temperature security activated
	 The preamplifier temperature is too high, the security fuse is activated, no
	 measurement available.
0x0b	 SENSOR: Out of range measurement
	 The measurement is out of range.
0x0c	 SENSOR: ok
	 The probe is working as expected.
0x0d	 Biomass simulator connected
	 The calibration cap is connected to the preamplifier.
0x0e	 SENSOR: total sterilization time overflow
	 The automatic sterilization counter has reached the maximum value (100)
0x0f	 SENSOR: overtime reached-calib/check needed
	 The sensor has been used more than 1 year since the last calibration.
	 Please perform a calibration.
0x10	 AMPLIFIER: overtime reached/check needed
	 The amplifier has been used more than 1 year since the last calibration.
	 Please perform a calibration.
0x11	 AMPLIFIER: not found
	 No preamplifier is connected to the channel.
0x12	 bootloader
	 Amplifier firmware updating
Table 21: Return values of the pre-amplifier
CONNECTION OF THE CONTROL UNIT
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CONNECTION OF THE CONTROL UNIT
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175Cell Density Monitoring System Operating Instructions
9	 FScan Frequency	 Byte 34…37: Frequency 15 (226/2)	 Byte 34…45: 0x00
	 15-17 reading	 Byte 38…41: Frequency 16 (228/2)
		 Byte 42…45: Frequency 17 (230/2)	
10	 FScan Frequency	 Byte 34…37: Frequency 18 (232/2)	 Byte 34…45: 0x00
	 18-20 reading	 Byte 38…41: Frequency 19 (234/2)
		 Byte 42…45: Frequency 20 (236/2)	
11	 FScan Frequency	 Byte 34…37: Frequency 21 (238/2)	 Byte 34…45: 0x00
	 21-23 reading	 Byte 38…41: Frequency 22 (240/2)
		 Byte 42…45: Frequency 23 (242/2)
12	 FScan Frequency	 Byte 34…37: Frequency 24 (244/2)	 Byte 34…45: 0x00
	 24-26 reading	 Byte 38…41: Frequency 25 (246/2)
		 Byte 42…45: Frequency 26 (248/2)
13	 FScan Frequency	 Byte 34…37: Frequency 27 (250/2)	 Byte 34…45: 0x00
	 27-29 reading	 Byte 38…41: Frequency 28 (252/2)
		 Byte 42…45: Frequency 29 (254/2)
14	 FScan-Values	 Byte 34…37: FScan 0 (256/2)	 Byte 34…45: 0x00
	 0-2 reading	 Byte 38…41: FScan 1 (258/2)
		 Byte 42…45: FScan 2 (260/2)
15	 FScan-Values	 Byte 34…37: FScan 3 (262/2)	 Byte 34…45: 0x00
	 3-5 reading	 Byte 38…41: FScan 4 (264/2)
		 Byte 42…45: FScan 5 (266/2)
16	 FScan-Values	 Byte 34…37: FScan 6 (268/2)	 Byte 34…45: 0x00
	 6-8 reading	 Byte 38…41: FScan 7 (270/2)
		 Byte 42…45: FScan 8 (272/2)
17	 FScan-Values	 Byte 34…37: FScan 9 (274/2)	 Byte 34…45: 0x00
	 9-11 reading	 Byte 38…41: FScan 10 (276/2)
		 Byte 42…45: FScan 11 (278/2)
18	 FScan-Values	 Byte 34…37: FScan 12 (280/2)	 Byte 34…45: 0x00
	 12-14 reading	 Byte 38…41: FScan 13 (282/2)
		 Byte 42…45: FScan 14 (284/2)
19	 FScan-Values	 Byte 34…37: FScan 15 (286/2)	 Byte 34…45: 0x00
	 15-17 reading	 Byte 38…41: FScan 16 (288/2)
		 Byte 42…45: FScan 17 (290/2)	
20	 FScan-Values	 Byte 34…37: FScan 18 (292/2)	 Byte 34…45: 0x00
	 18-20 reading	 Byte 38…41: FScan 19 (294/2)
		 Byte 42…45: FScan 20 (296/2)	
21	 FScan-Values	 Byte 34…37: FScan 21 (298/2)	 Byte 34…45: 0x00
	 21-23 reading	 Byte 38…41: FScan 22 (300/2)
		 Byte 42…45: FScan 23 (302/2)
22	 FScan-Values	 Byte 34…37: FScan 24 (304/2)	 Byte 34…45: 0x00
	 24-26 reading	 Byte 38…41: FScan 25 (306/2)
		 Byte 42…45: FScan 26 (308/2)
23	 FScan-Values	 Byte 34…37: FScan 27 (310/2)	 Byte 34…45: 0x00
	 27-29 reading	 Byte 38…41: FScan 28 (312/2)
		 Byte 42…45: FScan 29 (314/2)
NOTE: Some status codes are only available for Incyte pre-amplifiers.
For more details about the pre-amplifier status, see the Operating Instructions Manual available
at www.hamiltoncompany.com
12.4.2.5	 Function codes
In the following tables, the integration of each channel with the function code in the output buffer
is defined. The Modbus registers and lengths are written in brackets.
Table for Channel 1:
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Code	 Function	 Sensor type	 Sensor type
		 Channel 1 = 1 (Incyte)	 Channel 1 = 2 (Dencytee)
0	 No Function	 Byte 34…45: 0x00	
1	 Serial number	 Byte 34…45: Gateway serial number (1/6)
	 Arc View reading	
2	 Serial number	 Byte 34…45: Sensor serial number (7/6)	 Byte 34…45: Sensor serial
	 Sensor reading		 number (699/6)
3	 Serial number	 Byte 34…45: Amplifier serial number (13/6)	 Byte 34…45: Amplifier
	 Preamp reading		 serial number (705/6)
4	 FScan Frequency	 Byte 34…37: Frequency 0 (196/2)	 Byte 34…45: 0x00
	 0-2 reading	 Byte 38…41: Frequency 1 (198/2)
		 Byte 42…45: Frequency 2 (200/2)	
5	 FScan Frequency	 Byte 34…37: Frequency 3 (202/2)	 Byte 34…45: 0x00
	 3-5 reading	 Byte 38…41: Frequency 4 (204/2)
		 Byte 42…45: Frequency 5 (206/2)	
6	 FScan Frequency	 Byte 34…37: Frequency 6 (208/2)	 Byte 34…45: 0x00
	 6-8 reading	 Byte 38…41: Frequency 7 (210/2)
		 Byte 42…45: Frequency 8 (212/2)	
7	 FScan Frequency	 Byte 34…37: Frequency 9 (214/2)	 Byte 34…45: 0x00
	 9-11 reading	 Byte 38…41: Frequency 10 (216/2)
		 Byte 42…45: Frequency 11 (218/2)	
8	 FScan Frequency	 Byte 34…37: Frequency 12 (220/2)	 Byte 34…45: 0x00
	 12-14 reading	 Byte 38…41: Frequency 13 (222/2)
		 Byte 42…45: Frequency 14 (224/2)
Table 22: Function Codes Channel 1
177Cell Density Monitoring System Operating Instructions
Code	 Function	 Sensor type	 Sensor type
		 Channel 2 = 1 (Incyte)	 Channel 2 = 2 (Dencytee)
0	 No Function	 Byte 80…91: 0x00	
1	 Serial number	 Byte 80…91: Gateway serial number (1/6)
	 Arc View reading		
2	 Serial number	 Byte 80…91: Sensor serial number (53/6)	 Byte 80…91: Sensor serial
	 Sensor reading		 number (746/6)
3	 Serial number	 Byte 80…91: Amplifier serial number (59/6)	 Byte 80…91: Amplifier
	 Preamp reading 		 serial number (752/6)
4	 FScan Frequency	 Byte 80…83: Frequency 0 (321/2)	 Byte 80…91: 0x00
	 0-2 reading	 Byte 84…87: Frequency 1 (323/2)
		 Byte 88…91: Frequency 2 (325/2)
5	 FScan Frequency	 Byte 80…83: Frequency 3 (327/2)	 Byte 80…91: 0x00
	 3-5 reading	 Byte 84…87: Frequency 4 (329/2)
		 Byte 88…91: Frequency 5 (331/2)
6	 FScan Frequency	 Byte 80…83: Frequency 6 (333/2)	 Byte 80…91: 0x00
	 6-8 reading	 Byte 84…87: Frequency 7 (335/2)
		 Byte 88…91: Frequency 8 (337/2)
7	 FScan Frequency	 Byte 80…83: Frequency 9 (339/2)	 Byte 80…91: 0x00
	 9-11 reading	 Byte 84…87: Frequency 10 (341/2)
		 Byte 88…91: Frequency 11 (343/2)	
8	 FScan Frequency	 Byte 80…83: Frequency 12 (345/2)	 Byte 80…91: 0x00
	 12-14 reading	 Byte 84…87: Frequency 13 (347/2)
		 Byte 88…91: Frequency 14 (349/2)
9	 FScan Frequency	 Byte 80…83: Frequency 15 (351/2)	 Byte 80…91: 0x00
	 15-17 reading	 Byte 84…87: Frequency 16 (353/2)
		 Byte 88…91: Frequency 17 (355/2)
10	 FScan Frequency	 Byte 80…83: Frequency 18 (357/2)	 Byte 80…91: 0x00
	 18-20 reading	 Byte 84…87: Frequency 19 (359/2)
		 Byte 88…91: Frequency 20 (361/2)
11	 FScan Frequency	 Byte 80…83: Frequency 21 (363/2)	 Byte 80…91: 0x00
	 21-23 reading	 Byte 84…87: Frequency 22 (365/2)
		 Byte 88…91: Frequency 23 (367/2)
12	 FScan Frequency	 Byte 80…83: Frequency 24 (369/2)	 Byte 80…91: 0x00
	 24-26 reading	 Byte 84…87: Frequency 25 (371/2)
		 Byte 88…91: Frequency 26 (373/2)
13	 FScan Frequency	 Byte 80…83: Frequency 27 (375/2)	 Byte 80…91: 0x00
	 27-29 reading	 Byte 84…87: Frequency 28 (377/2)
		 Byte 88…91: Frequency 29 (379/2)
Table for Channel 2:
14	 FScan-Values	 Byte 80…83: FScan 0 (381/2)	 Byte 80…91: 0x00
	 0-2 reading	 Byte 84…87: FScan 1 (383/2)
		 Byte 88…91: FScan 2 (385/2)
15	 FScan-Values	 Byte 80…83: FScan 3 (387/2)	 Byte 80…91: 0x00
	 3-5 reading	 Byte 84…87: FScan 4 (389/2)
		 Byte 88…91: FScan 5 (391/2)	
16	 FScan-Values	 Byte 80…83: FScan 6 (393/2)	 Byte 80…91: 0x00
	 6-8 reading	 Byte 84…87: FScan 7 (395/2)
		 Byte 88…91: FScan 8 (397/2)
17	 FScan-Values	 Byte 80…83: FScan 9 (399/2)	 Byte 80…91: 0x00
	 9-11 reading	 Byte 84…87: FScan 10 (401/2)
		 Byte 88…91: FScan 11 (403/2)
18	 FScan-Values	 Byte 80…83: FScan 12 (405/2)	 Byte 80…91: 0x00
	 12-14 reading	 Byte 84…87: FScan 13 (407/2)
		 Byte 88…91: FScan 14 (409/2)
19	 FScan-Values	 Byte 80…83: FScan 15 (411/2)	 Byte 80…91: 0x00
	 15-17 reading	 Byte 84…87: FScan 16 (413/2)
		 Byte 88…91: FScan 17 (415/2)
20	 FScan-Values	 Byte 80…83: FScan 18 (417/2)	 Byte 80…91: 0x00
	 18-20 reading	 Byte 84…87: FScan 19 (419/2)
		 Byte 88…91: FScan 20 (421/2)
21	 FScan-Values	 Byte 80…83: FScan 21 (423/2)	 Byte 80…91: 0x00
	 21-23 reading	 Byte 84…87: FScan 22 (425/2)
		 Byte 88…91: FScan 23 (427/2)
22	 FScan-Values	 Byte 80…83: FScan 24 (429/2)	 Byte 80…91: 0x00
	 24-26 reading	 Byte 84…87: FScan 25 (431/2)
		 Byte 88…91: FScan 26 (433/2)
23	 FScan-Values	 Byte 80…83: FScan 27 (435/2)	 Byte 80…91: 0x00
	 27-29 reading	 Byte 84…87: FScan 28 (437/2)
		 Byte 88…91: FScan 29 (439/2)
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CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
Table 23: Function Codes Channel 2
179Cell Density Monitoring System Operating Instructions
Code	 Function	 Sensor type	 Sensor type
		 Channel 3 = 1 (Incyte)	 Channel 3 = 2 (Dencytee)
0	 No Function	 Byte 126…137: 0x00	
1	 Serial number	 Byte 126…137: Gateway serial number (1/6)
	 Arc View reading
2	 Serial number	 Byte 126…137: Sensor serial number (99/6)	 Byte 126…137: Sensor
	 Sensor reading 		 serial number (793/6)
3	 Serial number	 Byte 126…137: Amplifier serial number	 Byte 126…137: Amplifier
	 Preamp reading	 (105/6)	 serial number (799/6)
4	 FScan Frequency	 Byte 126…129: Frequency 0 (446/2)	 Byte 126…137: 0x00
	 0-2 reading	 Byte 130…133: Frequency 1 (448/2)
		 Byte 134…137: Frequency 2 (450/2)
5	 FScan Frequency	 Byte 126…129: Frequency 3 (452/2)	 Byte 126…137: 0x00
	 3-5 reading	 Byte 130…133: Frequency 4 (454/2)
		 Byte 134…137: Frequency 5 (456/2)
6	 FScan Frequency	 Byte 126…129: Frequency 6 (458/2)	 Byte 126…137: 0x00
	 6-8 reading	 Byte 130…133: Frequency 7 (460/2)
		 Byte 134…137: Frequency 8 (462/2)
7	 FScan Frequency	 Byte 126…129: Frequency 9 (464/2)	 Byte 126…137: 0x00
	 9-11 reading	 Byte 130…133: Frequency 10 (466/2)
		 Byte 134…137: Frequency 11 (468/2)
8	 FScan Frequency	 Byte 126…129: Frequency 12 (470/2)	 Byte 126…137: 0x00
	 12-14 reading	 Byte 130…133: Frequency 13 (472/2)
		 Byte 134…137: Frequency 14 (474/2)
9	 FScan Frequency	 Byte 126…129: Frequency 15 (476/2)	 Byte 126…137: 0x00
	 15-17 reading	 Byte 130…133: Frequency 16 (478/2)
		 Byte 134…137: Frequency 17 (480/2)
10	 FScan Frequency	 Byte 126…129: Frequency 18 (482/2)	 Byte 126…137: 0x00
	 18-20 reading	 Byte 130…133: Frequency 19 (484/2)
		 Byte 134…137: Frequency 20 (486/2)
11	 FScan Frequency	 Byte 126…129: Frequency 21 (488/2)	 Byte 126…137: 0x00
	 21-23 reading	 Byte 130…133: Frequency 22 (490/2)
		 Byte 134…137: Frequency 23 (492/2)
12	 FScan Frequency	 Byte 126…129: Frequency 24 (494/2)	 Byte 126…137: 0x00
	 24-26 reading	 Byte 130…133: Frequency 25 (496/2)
		 Byte 134…137: Frequency 26 (498/2)
13	 FScan Frequency	 Byte 126…129: Frequency 27 (500/2)	 Byte 126…137: 0x00
	 27-29 reading	 Byte 130…133: Frequency 28 (502/2)
		 Byte 134…137: Frequency 29 (504/2)
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
Table for Channel 3:
14	 FScan-Values	 Byte 126…129: FScan 0 (506/2)	 Byte 126…137: 0x00
	 0-2 reading	 Byte 130…133: FScan 1 (508/2)
		 Byte 134…137: FScan 2 (510/2)
15	 FScan-Values	 Byte 126…129: FScan 3 (512/2)	 Byte 126…137: 0x00
	 3-5 reading	 Byte 130…133: FScan 4 (514/2)
		 Byte 134…137: FScan 5 (516/2)
16	 FScan-Values	 Byte 126…129: FScan 6 (518/2)	 Byte 126…137: 0x00
	 6-8 reading	 Byte 130…133: FScan 7 (520/2)
		 Byte 134…137: FScan 8 (522/2)
17	 FScan-Values	 Byte 126…129: FScan 9 (524/2)	 Byte 126…137: 0x00
	 9-11 reading	 Byte 130…133: FScan 10 (526/2)
		 Byte 134…137: FScan 11 (528/2)
18	 FScan-Values	 Byte 126…129: FScan 12 (530/2)	 Byte 126…137: 0x00
	 12-14 reading	 Byte 130…133: FScan 13 (532/2)
		 Byte 134…137: FScan 14 (534/2)
19	 FScan-Values	 Byte 126…129: FScan 15 (536/2)	 Byte 126…137: 0x00
	 15-17 reading	 Byte 130…133: FScan 16 (538/2)
		 Byte 134…137: FScan 17 (540/2)
20	 FScan-Values	 Byte 126…129: FScan 18 (542/2)	 Byte 126…137: 0x00
	 18-20 reading	 Byte 130…133: FScan 19 (544/2)
		 Byte 134…137: FScan 20 (546/2)
21	 FScan-Values	 Byte 126…129: FScan 21 (548/2)	 Byte 126…137: 0x00
	 21-23 reading	 Byte 130…133: FScan 22 (550/2)
		 Byte 134…137: FScan 23 (552/2)
22	 FScan-Values	 Byte 126…129: FScan 24 (554/2)	 Byte 126…137: 0x00
	 24-26 reading	 Byte 130…133: FScan 25 (556/2)
		 Byte 134…137: FScan 26 (558/2)
23	 FScan-Values	 Byte 126…129: FScan 27 (560/2)	 Byte 126…137: 0x00
	 27-29 reading	 Byte 130…133: FScan 28 (562/2)
		 Byte 134…137: FScan 29 (564/2)
Table 24: Function Codes Channel 3
181Cell Density Monitoring System Operating Instructions
Code	 Function	 Sensor type	 Sensor type
		 Channel 4 = 1 (Incyte)	 Channel 4 = 2 (Dencytee)
0	 No Function	 Byte 172…183: 0x00	
1	 Serial number	 Byte 172…183: Gateway serial number (1/6)
	 Arc View reading
2	 Serial number	 Byte 172…183: Sensor serial number	 Byte 172…183: Sensor
	 Sensor reading	 (145/6)	 serial number (840/6)
3	 Serial number	 Byte 172…183: Amplifier serial number	 Byte 172…183: Amplifier
	 Preamp reading	 (151/6)	 serial number (846/6)
4	 FScan Frequency	 Byte 172…175: Frequency 0 (571/2)	 Byte 172…183: 0x00
	 0-2 reading	 Byte 176…179: Frequency 1 (573/2)
		 Byte 180…183: Frequency 2 (575/2)
5	 FScan Frequency	 Byte 172…175: Frequency 3 (577/2)	 Byte 172…183: 0x00
	 3-5 reading	 Byte 176…179: Frequency 4 (579/2)
		 Byte 180…183: Frequency 5 (581/2)
6	 FScan Frequency	 Byte 172…175: Frequency 6 (583/2)	 Byte 172…183: 0x00
	 6-8 reading	 Byte 176…179: Frequency 7 (585/2)
		 Byte 180…183: Frequency 8 (587/2)	
7	 FScan Frequency	 Byte 172…175: Frequency 9 (589/2)	 Byte 172…183: 0x00
	 9-11 reading	 Byte 176…179: Frequency 10 (591/2)
		 Byte 180…183: Frequency 11 (593/2)
8	 FScan Frequency	 Byte 172…175: Frequency 12 (595/2)	 Byte 172…183: 0x00
	 12-14 reading	 Byte 176…179: Frequency 13 (597/2)
		 Byte 180…183: Frequency 14 (599/2)
9	 FScan Frequency	 Byte 172…175: Frequency 15 (601/2)	 Byte 172…183: 0x00
	 15-17 reading	 Byte 176…179: Frequency 16 (603/2)
		 Byte 180…183: Frequency 17 (605/2)
10	 FScan Frequency	 Byte 172…175: Frequency 18 (607/2)	 Byte 172…183: 0x00
	 18-20 reading	 Byte 176…179: Frequency 19 (609/2)
		 Byte 180…183: Frequency 20 (611/2)
11	 FScan Frequency	 Byte 172…175: Frequency 21 (613/2)	 Byte 172…183: 0x00
	 21-23 reading	 Byte 176…179: Frequency 22 (615/2)
		 Byte 180…183: Frequency 23 (617/2)
12	 FScan Frequency	 Byte 172…175: Frequency 24 (619/2)	 Byte 172…183: 0x00
	 24-26 reading	 Byte 176…179: Frequency 25 (621/2)
		 Byte 180…183: Frequency 26 (623/2)
13	 FScan Frequency	 Byte 172…175: Frequency 27 (625/2)	 Byte 172…183: 0x00
	 27-29 reading 	 Byte 176…179: Frequency 28 (627/2)
		 Byte 180…183: Frequency 29 (629/2)
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
CONNECTION OF THE CONTROL UNIT
TO THE PROCESS CONTROL SYSTEM
Table for Channel 4:
14	 FScan-Values	 Byte 172…175: FScan 0 (631/2)	 Byte 172…183: 0x00
	 0-2 reading	 Byte 176…179: FScan 1 (633/2)
		 Byte 180…183: FScan 2 (635/2)
15	 FScan-Values	 Byte 172…175: FScan 3 (637/2)	 Byte 172…183: 0x00
	 3-5 reading	 Byte 176…179: FScan 4 (639/2)
		 Byte 180…183: FScan 5 (641/2)
16	 FScan-Values	 Byte 172…175: FScan 6 (643/2)	 Byte 172…183: 0x00
	 6-8 reading	 Byte 176…179: FScan 7 (645/2)
		 Byte 180…183: FScan 8 (647/2)
17	 FScan-Values	 Byte 172…175: FScan 9 (649/2)	 Byte 172…183: 0x00
	 9-11 reading	 Byte 176…179: FScan 10 (651/2)
		 Byte 180…183: FScan 11 (653/2)
18	 FScan-Values	 Byte 172…175: FScan 12 (655/2)	 Byte 172…183: 0x00
	 12-14 reading	 Byte 176…179: FScan 13 (657/2)
		 Byte 180…183: FScan 14 (659/2)
19	 FScan-Values	 Byte 172…175: FScan 15 (661/2)	 Byte 172…183: 0x00
	 15-17 reading	 Byte 176…179: FScan 16 (663/2)
		 Byte 180…183: FScan 17 (665/2)
20	 FScan-Values	 Byte 172…175: FScan 18 (667/2)	 Byte 172…183: 0x00
	 18-20 reading	 Byte 176…179: FScan 19 (669/2)
		 Byte 180…183: FScan 20 (671/2)
21	 FScan-Values	 Byte 172…175: FScan 21 (673/2)	 Byte 172…183: 0x00
	 21-23 reading	 Byte 176…179: FScan 22 (675/2)
		 Byte 180…183: FScan 23 (677/2)
22	 FScan-Values	 Byte 172…175: FScan 24 (679/2)	 Byte 172…183: 0x00
	 24-26 reading	 Byte 176…179: FScan 25 (681/2)
		 Byte 180…183: FScan 26 (683/2)
23	 FScan-Values	 Byte 172…175: FScan 27 (685/2)	 Byte 172…183: 0x00
	 27-29 reading	 Byte 176…179: FScan 28 (687/2)
		 Byte 180…183: FScan 29 (689/2)
Table 25: Function Codes Channel 4
183Cell Density Monitoring System Operating Instructions
SOFTWARE VERSIONS
HARDWARE COMPATIBILITY
12.4.3	 Modbus Interface
12.4.3.1	Configuration
The Arc View Controller Modbus Communication needs to be configured as described in
Chapter 12.1.
13	Disposal
The design of Hamilton Sensors optimally considers environmental compatibility.
In accordance with the EC guideline 2012/19/EU Hamilton Sensors, pre-amplifier
and Controller that are worn out or no longer required must be sent to a dedicated
collection point for electrical and electronic devices, alternatively, must be sent to Hamilton for
disposal. Sensors must not be sent to an unsorted waste disposal point.
有害物質表，請參閱www.hamiltoncompany.com，
章節過程分析，符合性聲明
DISPOSAL
14	 Software Versions
Version	Description
3.1	 Hamilton branding and basic Arc Sensor integration (MB)
3.2	 Graph and Data Logging for Arc Sensors (MB)
3.3	 Manual configuration of "Network Settings", allowing Cell Factor < 1.0,
	 set time-zone offset, Graph and Data Logging for Arc Sensors (MB and BT)
3.4	 Enabling Incyte (LC) low conductivity calibration, OPC connection improved,
	 Dencytee external light detection improved
3.5	 Enabling Cell Density Integration Kit, Dencytee external light detection improved
3.6	 Improved error analysis and sterilization cycle counter. Dencytee external light
	 detection adjusted
3.7	 Implementation of an overall software stabilization, including:
	 - Protection against corruption of initialization and culture files
	 - Disabled function to download running culture files
	 - Possibility to delete corrupt files from the Arc View Controller
	 - Fix of export data procedure
	 - Possibility to perform a script recovery
	 - Implementation of an error-logger to collect status-data in case of software
	 malfunction
3.7.1	 General improvements to increase system stability based on the error-logger, which was
	 implemented in version 3.7. Corrections of the alarm output for the 4-20 mA Output Box
3.7.2	 General improvements to increase system stability. Tolerances and measured value of
	 verification are now available in verification report
185Cell Density Monitoring System Operating Instructions
ORDERING INFORMATION ORDERING INFORMATION
15	 Hardware Compatibility
ATTENTION! Fogale devices are only partially compatible with Hamilton
products.
Please do not combine Hamilton labelled Pre-Amplifier and sensors with a Control Unit having a
software version below 3.0.
PC Box Software (V 2.8) and licenses are not compatible with the ComBox and vice versa.
Evo200 and ibiomass cannot be updated with the current Hamilton Software.
16	 Ordering Information
16.1	Controller
Choose Controller	 Choose License*
243800	 Arc View 265	 Code	 Incyte License
243801	 Arc View 465	 1	 yes
243802	 Arc View 465 XL	 0	 no
243810	 Cell Density ComBox		 Code	 Incyte Scan License**
			 1	 yes
			 0	 no
				Code	 Dencytee License
				 1	 yes
				 0	 no
					Code	 OPC License
					 1	yes
					 0	no
2438xy						 Order Code
* Licenses are specific to the Control Unit / ** Requires Incyte License
16.2	 Cell Density Integration Kit
Ref	Description
243809	 Cell Density Integration Kit
16.3	Units
Ref	Description
243710	 Incyte Unit DN25 - SG
243711	 Incyte Unit DN25 - DG
243712	 Incyte Unit DN25 - DG BE
243700	 Incyte Unit DN12 - 120
243701	 Incyte Unit DN12 - 220
243702	 Incyte Unit DN12 - 320
243703	 Incyte Unit DN12 - 420
243704	 Incyte Unit - 120 LC
243705	 Incyte Unit - 220 LC
243755	 Dencytee Unit - 120
243756	 Dencytee Unit - 225
243757	 Dencytee Unit - 325
243758	 Dencytee Unit - 425
187Cell Density Monitoring System Operating Instructions
ORDERING INFORMATION GLOSSARY
16.4	 Spare Parts and Accessories
Ref	Description
242379	 I-PC for CDIK
242380	 CDIK ComBox
243850	 4-20 mA Output Box
243851	 5 m cable M12 / open end
243852	 10 m cable M12 / open end
243720	 Incyte Pre-Amp
243730	 Incyte Sensor DN25 - SG
243731	 Incyte Sensor DN25 - DG
243732	 Incyte Sensor DN12 - 120
243733	 Incyte Sensor DN12 - 220
243734	 Incyte Sensor DN12 - 320
243735	 Incyte Sensor DN12 - 420
243736	 Incyte Sensor DN25 - DG BE
243716	 Incyte Sensor - 120 LC
243717	 Incyte Sensor - 220 LC
243760	 Dencytee Pre-Amp
243750	 Dencytee Sensor - 120
243751	 Dencytee Sensor - 225
243752	 Dencytee Sensor - 325
243753	 Dencytee Sensor - 425
243870	 5 m cable M12 / M12
243871	 10 m cable M12 / M12
243872	 20 m cable M12 / M12
243873	 40 m cable M12 / M12
243820	 OPC License (OPC XML-DA)
243822	 Incyte License
243823	 Incyte Scan License
243824	 Dencytee License
243883	 Arc View Controller Power Supply
243889	 Arc View Controller Modbus-Profibus DP module
16.5	 Calibration Tools
Ref	Description
243740	 Incyte Val/Cal Kit
	 Sol A, Sol B, Capacitance Simulator
243713	 Incyte Val/Cal Kit LC
	 Sol A, Sol B (LC), Capacitance Simulator
238988	 Sol A for Incyte Calibration
243742	 Sol B for Incyte Calibration
243708	 Sol B for Incyte Calibration LC
243743	 Capacitance Simulator
243886	 Dencytee Val/Cal Sol
17	Glossary
Name	Description
Unit	 Refers to sensor and pre-amplifier calibrated onto each other
Probe	 Equivalent to sensor
Capacitance 	 Measurement of the viable cell density with the Incyte sensor,
measurement	 based on an alternating electric field
Optical density/ 	 Measurement of the total cell density with the Dencytee sensor,
turbidity measurement	 based on the turbidity of the medium
Incyte Scan/FScan/Scan	 Multifrequency scanning during capacitance measurements
Calibration	 Calibration of a sensor and a pre-amplifier, meaning not a
	 correlation to biological offline measurements
ComBox	 Refers to Cell Density ComBox
CDIK	 Refers to Cell Density Integration Kit
Table 21: Overview of relevant terms.
189Cell Density Monitoring System Operating Instructions
FAQ FAQ
18	FAQ
18.1	 General Questions
What is the Administrator Password?
The default Password is 14147125
How Should the Sensors be stored?
The recommended storage conditions are at 4 to 50°C and 80 % relative humidity. The Sensors
are very robust; they do not have to be stored in a specific container or liquid.
How often do I need to calibrate the Unit?
The Sensor comes factory-calibrated. A calibration procedure must be performed when the
Sensor or the Pre-amplifier is replaced.
What is the max. cable length between Unit and Arc View Controller?
The distance is theoretically unlimited because the signal is digital.
What is the effect of gas bubbles on the measurement?
Gas bubbles have two main impacts:
1. Decrease in the signal intensity, since the Sensor is not measuring in air
2. Increase of background noise
What is the effect of agitation variations on the measurement?
The agitation can influence the size of air bubbles. This number and size of air bubbles can
affect the Cell Density measurement
Can I detect microorganism contamination with the Sensors?
Yes, indirectly by the effect that the contamination has on the culture, for example a faster, or
slower growths, but a direct measure of the contaminants is not possible.
Do you measure % viability?
No. We measure the viable cell density with Incyte and total cell density with Dencytee. You can
graph both signals on one screen to analyze the deviation of viable and total cell density.
What is the effect of anti-foam agents on the measurement?
Antifoam has no effect on Incyte Sensors. Anti-foam may interfere with the turbidity measurement
of Dencytee, as it may scatter the light.
Is it possible to validate the system?
Yes, the system can be validated. Hamilton can provide IQ/OQ validation packages
(Ref 243999-07). The systems have already been validated for use in GMP environment with
several customers.
How big is the memory for data storage in the Arc View Controller?
The memory of the system is 16 GB which corresponds to one year of recording with a recording
period of 1 second (recording of full dataset, including spectroscopy data), or 60 years of
recording for a recording period of 1 minute.
Is the system software 21 CFR Part11 compliant?
No
Can I use the cell density signal to trigger actuator(s)?
Yes. You can configure the alarm outputs of the 4-20 mA Box to control pumps.
191Cell Density Monitoring System Operating Instructions
18.2	 Incyte Questions
How is the correlation Cell Factor calculated for the Incyte Sensor?
The system gives you the possibility to correlate the permittivity signal of the Incyte with a reference
measurement such as Viable Cell Count (cell/ml), OD or Dry Cell Weight (g/l). The correlated
value is referred to as the “Cell Density” value and is linked to the permittivity according to
the following equation: Cell Density = (Permittivity – Offset) x cell factor. In order to correlate the
permittivity signal with your reference measurements, you must determine the cell factor during
the exponential phase of a culture by simultaneously taking your offline reference measurements
and the permittivity readings. You can then build the linear relationship between the capacitance
measurements (x axis) and your reference measurements (y axis): the slope of the linear correlation
corresponds to the cell factor. The cell factor is cell line specific.
Can the electric field of Incyte damage/harm the cells?
No. The frequency of the electric field applied by the system is within 300 kHz and 10 MHz.
These frequencies do not damage the cells.
What is the effect of cell aggregates, cell clumping on the Incyte measurement?
The proximity of cells can induce a slight decrease in the capacitance signal, up to 3-4%. It is
also known that cell aggregates can create some noise on the signal which can be minimized
with the system integration function which is based on a moving average calculation.
When should I use the Incyte Scan?
All calculation done during the Incyte Scan are based on a spherical model. For this reason, this
scan works best is for cell culture and yeast applications. The spectroscopy module provides information
related to the physiological state of the cells. It may be used to monitor key phases of
processes that are typically characterized by major physiological changes in the cell population.
Can I use the Incyte Sensor with micro-carriers?
Yes, the Sensor is insensitive to micro-carriers. Incyte measures the bio volume of each cell on
the microcarrier.
FAQ FAQ
How is Incyte measurement influenced if the organisms are not round but cylindrical
or long stretched?
The measurement is possible but calculations are based on spherical cells. That means there
are 2 beta dispersion curves, one for the small dimension and one for the long dimension.
The signal is the combination of those 2 curves.
Are spores detected by Incyte Sensor?
Spores are not detectable because they do not have a plasma membrane, and are too small.
Will oil drops influence the measurement of Incyte?
Oil will most likely create a surface layer on the electrodes and that will affect calibration and
probably disturb the signal stability during the run. The effect at higher concentrations is not
significant.
What is the effect of changes in conductivity on the Incyte measurement?
Changes in conductivity during a culture do not impact the capacitance measurement. The
Sensor measures in a wide range of conductivity (2 to 50 mS/cm) and changes within that range
do not affect the capacitance measurements.
What is the effect of changes in pH on the Incyte measurement?
There is no effect from pH.
Can I use the Incyte Sensor in very high cell concentrations?
Yes, there is no limitation in the higher end of concentrations.
Can I use the Incyte Sensors for offline measurements?
Yes, the Sensor may be used in stirred beaker experiments. For optimal results, it is important to
make sure that there is at least 1 cm of free space around the Sensor to avoid any interactions
with the electric field. The integration/moving average function may be reduced to a low integration
or no integration for real-time measurements.
193Cell Density Monitoring System Operating Instructions
FAQ NOTES
What can I do, if a constant high offset (> 3 pF/cm) is observed?
Please make clear zero of the dual measurement and scan after the process or ensure to mark
zero prior to every inoculation.
What is the effect of solid particles on the Incyte measurement?
A content of solid particles higher than 10% (w/v) may interfere with the Incyte measurement, as
the particles disturb the electrical field and may create a noise level.
195Cell Density Monitoring System Operating Instructions
NOTES NOTES
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