Operating manual
eve® - the bioprocess platform software
Version: 1.102
2 version 1.102 | Operating manual eve®
Infors AG
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Engineering and production in Switzerland
Operating manual eve® | version 1.102 3
Contents
1 INTRODUCTION 7
1.1 Package structure 7
1.2 About this document 9
2 INSTALLATION 10
2.1 Requirements 10
2.1.1 Hardware 10
2.1.2 Operating system 11
2.1.3 System requirements 11
2.2 Installation 12
2.3 Software maintenance 15
2.4 First log-in 16
3 DASHBOARD 17
3.1 Dashboard screen 17
3.2 Main menus 18
3.3 Control keys 20
3.3.1 Menu structure appearance 21
3.3.2 Search and filter tables 23
3.3.2.1 Advanced filtering 23
4 PLAN & RUN 26
4.1 Single Batch 26
4.1.1 Basic setup 27
4.1.1.1 Project allocation 28
4.1.1.2 Load and apply recipe 28
4.1.1.3 Parameter configuration 29
4.1.1.4 Device Selection 35
4.1.2 Organism information 36
4.1.2.1 Select organism 38
4.1.2.2 Inoculum information 39
4.1.3 Culture media information 40
4.1.3.1 Culture media selection 41
4.1.3.2 Edit culture media compounds 42
4.1.4 Batch strategy 44
4.1.4.1 Phase configuration 46
4.1.4.2 Transition rules 47
4.1.4.3 Parameter set points 53
4.1.4.4 Predefined functions 54
4.2 Multiple Batch 61
4.2.1 Reference batch configuration 61
4.2.2 Multiple batch overview table 63
4.2.2.1 Change device 64
4.2.2.2 Change basic setup 65
4.2.2.3 Change organism information 66
4.2.2.4 Change culture media information 67
4.2.2.5 Change strategy 68
4.2.2.6 Start, open or delete a batch process 69
4.2.2.7 Custom functions and scripts 71
5 MONITOR & ANALYSE 73
5.1 Monitor a batch process 74
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5.1.1 Alarms 75
5.1.1.1 Subscribe to e-mail alarm notifications 76
5.1.1.2 Active alarm 77
5.1.1.3 Inactive alarm 77
5.2 Chart configuration 78
5.2.1 Axis configuration 79
5.2.1.1 Change parameter position 80
5.2.1.2 Show and hide all parameters 81
5.2.2 Parameter appearance 82
5.2.2.1 Change axis 82
5.2.2.2 Change marker 83
5.2.2.3 Change color 83
5.2.2.4 Change line size 83
5.2.3 Axis scale 84
5.2.3.1 Change min. and max. value 84
5.2.3.2 Change decimals of the axis scale: 85
5.2.4 Time scale and zoom 86
5.2.4.1 Change time scale 86
5.2.4.2 Change time since inoculation 87
5.2.4.3 Zoom navigation: 87
5.2.5 Tool tip mode 88
5.2.6 Refresh rate 90
5.3 Chart windows management 91
5.3.1 Add additional charts 92
5.4 Chart templates 93
5.4.1 Save a chart template 93
5.4.2 Apply a chart template 94
5.4.3 Delete a chart template 94
5.5 Chart export 95
5.5.1 Print chart 96
5.6 Batch comparison 96
5.6.1 Select batch processes 96
5.6.2 Compare batch processes 98
5.6.3 Synchronise batch processes 99
5.6.4 Switch between compared batch processes 100
5.7 Operator-mediated batch adjustments 101
5.7.1 Inoculate batch process 102
5.7.2 Offline samples 103
5.7.3 Logbook entry 105
5.7.4 Parameter set points 105
5.7.5 Change strategy 106
5.7.6 Skip phase manually 106
5.7.7 Stop batch 107
6 LIBRARIES 108
6.1 Batches 109
6.1.1 Archive data 110
6.1.2 Import data 111
6.1.3 Export data 112
6.1.4 Report 112
6.2 Recipe 117
6.2.1 Create new recipe 117
6.2.2 Apply recipes 117
6.2.3 Import Recipes 118
6.2.4 Export Recipes 119
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6.3 Organism 120
6.3.1 Add new organism 120
6.3.2 Edit organism 122
6.3.3 Delete an organism 122
6.4 Culture media 123
6.4.1 Add new culture medium 124
6.4.2 Edit culture medium 126
6.4.3 Delete culture media 127
6.5 Compounds 128
6.5.1 Add new compound 129
6.5.2 Edit compound 130
6.5.3 Delete compound 130
6.6 Soft-sensors 131
6.6.1 Soft-sensor basics 131
6.6.2 Add new soft-sensor 135
6.6.3 Apply soft-sensor 140
6.6.3.1 Running batch 140
6.6.3.2 Completed batch 141
6.6.4 Edit a soft-sensor 142
6.6.5 Delete a soft-sensor 143
6.7 Audit trail 144
6.7.1 Export 147
7 RESOURCES 149
7.1 Equipment 149
7.1.1 Add new equipment 149
7.1.1.1 Select equipment 150
7.1.1.2 Configure Equipment 151
7.1.1.3 Edit equipment 151
7.1.2 Bind device to existing equipment 152
7.1.3 Delete device from existing equipment 155
7.1.4 Edit device 156
7.1.4.1 Additional parameter information 157
7.1.4.2 Change device parameter settings 158
7.1.4.3 Configure unknown device parameter161
7.2 Projects 162
7.2.1 Create new project 163
7.3 User administration 164
7.3.1 Add new user 166
7.3.2 Edit user 167
7.3.2.1 Activate/inactivate user 167
7.3.2.2 Define user role 167
7.3.2.3 Reset password 167
8 SETTINGS 169
8.1 My Profile 169
8.1.1 Change Password 170
8.1.2 Change profile picture 171
8.1.3 Enable e-mail alarm notification 172
8.2 Global 173
8.2.1 Batch settings 174
8.2.1.1 Default batch report settings 174
8.2.1.2 Mandatory information 175
8.2.2 Data settings 176
8.2.2.1 .csv export settings 176
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8.2.2.2 Custom basic setup metadata 177
8.2.2.3 Custom organism metadata 178
8.2.2.4 Custom culture media metadata 180
8.2.2.5 Backup 182
8.2.3 System settings 184
8.2.3.1 Automatic user log-off 184
8.2.3.2 User blocking after failed log-in 184
8.2.3.3 IP white-list 185
8.2.3.4 Password aging 185
8.2.3.5 High security password 185
8.2.3.6 E-Mail server configuration 186
8.2.3.7 REST API access 187
8.3 About 187
8.4 License 188
8.4.1 Activate license online 189
8.4.2 Activate license offline 190
9 GLOSSARY 192
INTRODUCTION
Operating manual eve® | version 1.102 7
1 Introduction
eve® the bioprocess platform software, is able to do more than just
plan, control and analyse your bioprocesses. eve® software integrates
work flows, devices, bioprocess information and big data in a
web-based platform that lets you organise your projects - no matter
how complex they are.
eve® integrates complex control strategies based on soft-sensors and
phase transition techniques and makes it accessible to non-expert
users via a modern intuitive work-flow. On-line and off-line data are
seamlessly integrated for comparison and graphical analysis in real
time.
eve® is the platform for big data in bioprocesses. It uses cutting-edge
NoSQL technology instead of traditional SQL. eve® catapults your
bioprocesses into the world of big data, making bioprocess information
centralised, unlimited and fast.
eve® software embraces incubator shakers and bioreactors of all
sizes and types and allows to connect and manage your lab
resources in a easy way.
1.1 Package structure
eve® has a modular structure and permits that only required capabilities
need to be specified and each user can build a tailored set of
functionality to meet their precise needs. The core software can be
expanded with additional packages.
eve® Core:
Plan and monitor experiments, including data storage and export.
– Work flow oriented planing and monitoring of up to 99 batches
– Connect any number of bioreactors and shakers*
– Save batch data in a central NoSQL database (Elastic)
– Preconfigured, individually adjustable charts
– Libraries for organising and administrating process data
– Dynamic and fixed alarms
– Data import: .csv, .xls, .xlsx, .eve, .iri
– Data export: .csv, .eve
– Supported clients: OPC XML DA, OPC DA, OPC UA
* Number depends on server performance
INTRODUCTION
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eve® Plan & Control:
Design complex strategies, control mechanisms and expanded ana-
lysis.
– Complete batch strategy system including preconfigured and programmable
functions
– Comparison charts: compare current with completed batches
(Golden Batch)
– Time- and event-based comparison between current and archived
batches in adaptable charts
– Set control values manually or through the aid of a function
(Strategy, etc.)
– Create soft sensors using integrated templates
– Plan multiple batches simultaneously in a single experiment
– Save experiment as recipes
eve® User Management & Reporting:
A perfect addition for people that would like to go beyond FDA part
11 compliance. Track, record and report all the actions in your batch
or system.
– Export batch reports of your planned experiments and/or batches
with all your recipe information
– Log of relevant changes and events (e.g. start batch, setpoints, offline
samples etc.)
– Log of changes in the software environment (e.g. configuration,
updates etc.)
– Log of changes in the User Management (e.g. new user, role etc.)
– Enhanced User Management with 5 user roles (System Administrator,
Manager, User, Operator and Guest)
– Automatic log-off
– High-security password and password aging
– Limit batch control to selected computer
INTRODUCTION
Operating manual eve® | version 1.102 9
1.2 About this document
This manual enables the safe and efficient handling of eve the bioprocess
platform software. This manual must be accessible to the operators
at all times. The operators must read thoroughly and fully
understand this manual before commencement of any work.
Adhering to all the points, advice and instructions concerning safety
and operation in this manual is a pre-condition for safe work.
This manual provides the information which are included in eve® the
bioprocess platform software with the following license con-
figuration:
– eve® Core
– eve® Plan & Control
– eve® User Management & Reporting
All of the figures in this manual showing the various menus, dialogue
boxes and pages of the eve bioprocess platform software reflect the
view of a user with the authorisation level of a Manager.
All relevant text statements about general information and direct
warnings in this manual are marked as follows:
This note gives a simple application example for a function
or setting in the bioprocess platform software eve
and indicates an easy solution for a specific demand.
This symbol indicates further information about a function
or setting in the bioprocess platform software eve.
This symbol indicates a hazard situation which could
result in malfunction of the software and/or data loss.
In addition, the following concept of lists will also be used:
– Normal lists use a simple dash
> Specific actions which must be carried out in this order use an
orange angle bracket
> Screens which appear and the results of other actions use a grey
angle bracket
[] Text inside square brackets refers to control elements (buttons)
INSTALLATION
Operating manual eve® | version 1.102 10
2 Installation
2.1 Requirements
2.1.1 Hardware
For a successful operation of eve the bioprocess platform software
the following minimal requirements have to be met:
System requirements for up to 6 devices:
Category Requirements
processor Intel i5 or above
memory 16 GB RAM
hard disc
250 GB SSD or SAS
(500 GB SSD recommended)
monitor resolution 1280 x 1024 (1920 x 1080 recommended)
network Gigabit Ethernet LAN adapter
operating system
64-bit, all systems
(see "Operating system" on the next page)
System requirements for up to 20 devices:
Category Requirements
processor Intel i7, i9 or Xeon (at least 4 cores)
memory 16 GB RAM (32 GB recommended)
hard disc 500 GB SSD or SAS
monitor resolution 1280 x 1024 (1920 x 1080 recommended)
network Gigabit Ethernet LAN adapter
operating system
64-bit, only Windows Server
(see "Operating system" on the next page)
System requirements for up to 80 devices:
Category Requirements
processor Intel Xeon (at least 8 cores)
memory 32 GB RAM (64 GB recommended)
hard disc 1 TB SSD or SAS
monitor resolution 1280 x 1024 (1920 x 1080 recommended)
network Gigabit Ethernet LAN adapter
operating system
64-bit, only Windows Server (see "Operating
system" on the next page)
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2.1.2 Operating system
To install and run eve® the bioprocess platform software, one of the
following operating systems (64bit) has to be installed:
– Windows 7 Professional
– Windows 10 Pro
– Windows Server 2012 R2 Standard
– Windows Server 2016
In addition, we recommend one of the following web browser:
– Chrome
– Edge
– Internet Explorer 11
Please avoid any additional applications that are using
a lot of resources (CPU, memory, I/O, Bandwidth etc.).
2.1.3 System requirements
Before you install eve ® the bioprocess platform software please
ensure that the following requirements are met:
– Port 80 and 8080 must not be used or blocked
– No pre installed version of elasticsearch data base
During the installation of eve ® the computer must
have access to the internet.
INSTALLATION
Operating manual eve® | version 1.102 12
2.2 Installation
> Click on the eve Setup.exe file.
> The Welcome to the InstallShield Wizard for eve screen appears.
> Click the [NEXT] button at the bottom of the welcome screen.
> The license agreement screen appears.
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> Click the "I accept the terms in the license agreement" and click
the [NEXT] button at the bottom of the license agreement screen.
> The custom setup screen appears.
> Click the [INSTALL] button at the bottom of the custom setup
screen.
> All the program features are then being installed.
> During the installation the installer needs a reboot before the configuration
of eve® can continue.
INSTALLATION
Operating manual eve® | version 1.102 14
> Click the yes button in order to restart the computer.
> After restart the InstahllShieldWizard completed screen appears.
> Click the [FINISH] button at the bottom of the InstallShield Wizard
completed screen.
> eve® the bioprocess platform software has been installed suc-
cessfully.
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2.3 Software maintenance
In order to update, repair or uninstall eve® the bioprocess platform
software proceed as follows:
Update:
> Click on the newly received eve Setup.exe file.
> Follow the same instructions as for the first installation (see "Installation"
on page 12).
Repair and uninstall:
> Click on the eve Setup.exe file which was originally used for the
first installation.
> The program maintenance screen appears.
> Click on the corresponding orange field (repair or uninstall) and
continue with the process.
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Operating manual eve® | version 1.102 16
2.4 First log-in
After a successful installation of eve® the software can be started as
follows:
> Click on the launch eve icon which is available on your desktop.
> The default web browser of your operating systems starts and the
eve® login-in screen appears.
For the first log-in please use the default user name:
User name: Administrator
Password: Administrator
> Enter your user name and the corresponding password.
> Click the [LOGIN] button.
> The DASHBOARD screen appears.
Administrator are only allowed to manage the equipment
and user accounts (see "Resources" on
page 149). Full feature access is exclusively activated
for users with a role as a manager or user.
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3 Dashboard
The dashboard is the main overview window of eve® the bioprocess
platform software. The widget-like tiles of running and planned
batch processes support a fast, easy control and access of the experiments
and batch processes.
3.1 Dashboard screen
Number Function
1 Display of current position
2 Main menu structure
3 Running batch overview and control table
4 Planned experiment overview and control table
5 Audit trail overview
6 Help (eve documentation)
7 Full screen mode
8 Log out of software
9 User profile
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Operating manual eve® | version 1.102 18
3.2 Main menus
PLAN & RUN:
In the PLAN & RUN menu single and multiple batch processes can be
planned and started. The following functions are available:
– Clean and intuitive work flow procedure for batch processes
– Connection to a comprehensive organism, culture media and softsensor
library
– Revolutionary strategy design tool for batch processes with integrated
functions and soft-sensors
MONITOR & ANALYSE:
In the MONITOR & ANALYSE menu all batch processes can be monitored,
controlled and analysed. The following functions are avail-
able:
– Monitor and control batch processes
– Compare live and historical batch data
– Advanced chart customisation
LIBRARIES:
In the LIBRARIES menu all the stored information about, batches,
recipes, organisms, culture media, soft-sensors and audit is accessible.
The following functions are available:
– Import and export of batch data
– Creation of organism, culture media and compounds library
– Built-in soft-sensor scripting engine
– Audit trail on user actions
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RESOURCES:
In the RESOURCES menu the configuration of equipment, projects
and users can be managed. The following functions are available:
– Quick and easy device connection
– Project and data structuring
– User management
SETTINGS:
In the SETTINGS menu the configuration of user specific and global
settings can be changed. In addition, information about the software
and the license configuration is provided. The following functions are
available:
– Profile customisation
– Log-off and data export configuration
– Software information
– License configuration
DASHBOARD
Operating manual eve® | version 1.102 20
3.3 Control keys
Control key Description
Help
Full screen
Windowed screen
Log out
User profile
Open (planned) batch
Open (completed and running) batch
Export
Report
Edit
Delete
Settings
Load
Expand
Minimise
Add
Close
Time
Skip phase
Filter table
Archive
Binding (soft-sensor)
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In eve several options are available to improve the navigation and
work space behaviour in the software. By default on bottom the lefthand
side of the screen the menu structure is always minimised.
3.3.1 Menu structure appearance
To expand the menu structure, proceed as follows:
> Click the small arrow tab which is attached to the menu structure
in order to expand the menu structure.
> The entire menu structure is expanded and the menu titles are dis-
played.
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Operating manual eve® | version 1.102 22
> Click again the small arrow tab in order to minimise the menu struc-
ture.
> The menu structure is minimised again.
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3.3.2 Search and filter tables
Almost every table in eve® the bioprocess platform software can be
searched and filtered and allows you to search quickly for a specific
entry (e.g. culture media compound).
> Enter any term and click the [FILTER] button in order to look for a
specific entry.
> The entries of the table are filtered according to the entry.
> Click on any header in a table in order to search for a specific entry
or sort the entries in an ascending or descending order.
> The options for sort and filter appear.
> Click the header in order to sort the table in an ascending order.
> The entries of the table are in an ascending order.
> Click the header again in order to sort the table in a descending
order.
> The entries of the table are in a descending order.
3.3.2.1 Advanced filtering
Two data libraries namely, BATCH and AUDIT TRAIL have an
advanced search function. This search is a very powerful full-text
search function and is capable of searching for:
– Project names (e.g. EcoliGFP)
– Experiment names (e.g. GrowthExp001)
– Batch names (e.g. E_coli_001)
– Batch descriptions (e.g. growth studies of EColi)
– Device names (e.g. Labfors)
– Unit names (e.g. "B")
– Organism names (e.g. "Escherichia coli")
– Clone names (e.g. K-12)
– Culture media names (e.g. BasalSaltMedia)
– Culture media compound names (e.g. Glucose)
– Phase names (e.g. Fed-batch)
– Function names (e.g. linear)
– States (e.g. completed)
– User names (e.g. User1)
DASHBOARD
Operating manual eve® | version 1.102 24
– Event type (Audit Trail, e.g. Inoculate)
– Action (Audit Trail, e.g.
– Each query (search/filter) is a series of terms and operators.
– A term can be a single word - Escherichia or - coli or - a phrase surrounded
by double quotes - "Escherichia coli" - which searches for
all the words in the phrase, in the same order:
Escherichia coli
states that:
- Escherichia OR coli has to be present
– Wildcard searches can be run on individual terms, using ? to
replace a single character, and * to replace zero or more char-
acters:
Lab*
states that:
- Lab has to be present and more characters could fol-
low
– By default, all terms are optional, as long as one term matches.
– The preferred operators are + (this term must be present) and (this
term must not be present). All other terms are optional. For
example, this query:
Coli Labfors +K-12 -planned
states that:
- K-12 must be present
- Planned must not be present
- Coli and Labfors are optional - their presence
increases the relevance
The familiar operators AND, OR and NOT (also written &&, || and !)
are also supported. However, the effects of these operators can be
more complicated than is obvious at first glance. NOT takes precedence
over AND, which takes precedence over OR. While the +
and - only affect the term to the right of the operator, AND and OR
can affect the terms to the left and right.
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Rewriting the above query using AND, OR and NOT demonstrates
the power and complexity of this function:
Coli OR Labfors AND K-12 AND NOT planned
This is incorrect because Labfors is now a required
term.
(Coli OR Labfors) AND K-12 AND NOT planned
This is incorrect because at least one of coli or labfors is
now required and the search for those terms would be
scored differently from the original query.
((Coli AND K-12) OR (Labfors AND K-12) OR K-12)
AND NOT planned
This form now replicates the logic from the original
query correctly, but the relevance scoring bears little
resemblance to the original.
To execute such search/filter queries proceed as follows:
> Enter the search/filter term in the search entry field.
> Click the [SEARCH] (or enter) button.
> The query result is a list of all library entries matching the search/-
filter.
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4 Plan & Run
Bioprocesses can be either conducted in parallel or one at a time.
The bioprocess platform software eve allows you to chose your
desired work flow and then plan and run experiments and batches
easily.
The PLAN & RUN menu includes the following sub-menus and allows
you to configure your bioprocesses individually:
Menu Function
Single batch
- Experiments with one single batch process
- Configuration of all process parameters
- Supportive experiment work flow
- Detailed organism and culture media library
- Revolutionary strategy design tool
Multiple batch
- Reference batch for multiple batch applications
- Overview table of all planned batch processes
- Experiment with multiple batch processes
- Configuration of all process parameters
- Supportive experiment work flow
- Detailed organism and culture media library
- Revolutionary strategy design tool
In order to plan and run a batch process proceed as follows:
> Click the [PLAN & RUN] button on the left-hand side of the screen.
> The sub-menus of PLAN & RUN appear.
4.1 Single Batch
The SINGLE BATCH is a function which allows you to plan and run
individual batch processes. The entire work flow can be divided into
four steps. In each of the four work flow steps, the specific details
can be configured and used for your batch process. The icon of each
step indicate the position in the work flow and can be simultaneously
used as a navigation button. The four work flow steps are as follows:
– Basic setup (see "Basic setup" on the facing page)
– Organism (see "Organism information" on page 36)
– Culture media (see "Culture media information" on page 40)
– Strategy (see "Batch strategy" on page 44)
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Operating manual eve® | version 1.102 27
4.1.1 Basic setup
In the BASIC SETUP section generic information about the batch process
can be configured. In addition, the allocation to a project, loading
of recipes and the selection of the equipment with the
subsequent configuration of all the process parameters can be man-
aged.
> Click the [SINGLE BATCH] button on the left-hand side of the
PLAN & RUN menu.
> The BASIC SETUP screen appears.
Number Function
1 Current work flow position (BASIC SETUP)
2 Name of the batch process
3 Description of the batch process
4 Allocation to a project
5 Selection of a recipe
6 Selection of the device
7 Configuration of the process parameters
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4.1.1.1 Project allocation
> Click the icon to get a list of the stored projects.
> A list of the created projects appears.
> Select your appropriate project.
> The batch process is allocated to the specific project.
The allocation of a batch process to a any project is
only possible if a projects is created in the library (see
"Projects" on page 162). If no project was created the
allocation is by default a "no-name" project.
4.1.1.2 Load and apply recipe
> Click the icon in the LOAD RECIPEfield in order to select the
appropriate recipe for your batch process.
> The LOAD RECIPE pop-up screen appears.
> Select the corresponding recipe and click the [OK] button at the
bottom of the LOAD RECIPE pop-up screen.
> Depending on your recipe the entire work flow is configured.
If no recipe is selectable you have to create a recipe
from an existing batch (see "Create new recipe" on
page 117).
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Operating manual eve® | version 1.102 29
4.1.1.3 Parameter configuration
In the CONFIGURE PARAMETER section three different kinds of parameters
can be configured and selected:
– Device parameters (see "Device parameters:" on the next page)
– Offline parameters (see "Offline parameter:" on page 32)
– Soft-sensors (see "Soft-sensor:" on page 34)
Number Function
1 Control parameter on / off
2 Field for device parameter set point value
3 Field for lower alarm value
4 Field for upper alarm value
5
Fixed alarm type: Any specific value under or above a
set point which defines absolute boundary limits that
should not be reached.
6
Dynamic alarm type: A certain window which moves
with the set point (e.g. always ± 5 °C of the temperature
set point).
7 Alarm on / off
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Device parameters:
The configure device parameters allows you to set the following:
– Set Control parameter on / off(see "Control parameter on / off:"
below)
– Setpoints of device parameters (see "Setpoint of device parameters:"
on the facing page)
– Alarm type (see "Alarm type:" on the facing page)
– Limit and range of alarms (see "Limit and range of alarms:" on the
facing page)
– Set arlarm on / off (see "Alarm on / off:" on the facing page)
> Click the icon in the CONFIGURE PARAMETERS section in
order to configure your parameters.
> The CONFIGURE PARAMETERS pop-up screen appears.
> By default the list of device parameters is expanded.
Control parameter on / off:
In order to change the control of a specific parameter click directly
into device parameter list:
> Click on any control toggle button of a specific parameter.
> The control of the specific parameter is on / off.
If you click on the CONTROL column header itself you
can either switch all parameter control on or off sim-
ultaneously.
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Setpoint of device parameters:
In order to change a setpoint of a specific parameter click directly
into the device parameters list:
> Click into any setpoint field of a specific parameter and enter the
new value.
> The new setpoint of the parameter is set.
Alarm type:
In order to change the alarm type of a specific parameter click directly
into the device parameters list:
> Click either on the FIXED or DYNAMIC alarm type button.
> The alarm type of the specific parameter has changed an the lower
and upper alarms can be configured.
Limit and range of alarms:
Depending on the selected alarm type the ranges and limits of the
alarm can be set differently. In order to change the range or limit of a
specific parameter alarm click directly into the device parameters
list:
> Click into either the upper or lower alarm field of the specific para-
meter.
> Change the value either by entering a new value.
> The new range or limits of the alarm is set.
Alarm on / off:
In order to change if an alarm is enabled or disabled of a specific
parameter click directly into the device parameters list:
> Click on the OFF button of the specific parameter.
> The alarm of the specific parameter if on / off.
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Offline parameter:
Each batch process can have several configured offline parameters.
In order to add an offline parameter proceed as follows:
> Click the icon in the CONFIGURE PARAMETERS section in
order to configure your parameters.
> The CONFIGURE PARAMETERS pop-up screen appears.
> By default the list of device parameters is expanded.
> Click the offline parameters tab in order to add an offline para-
meter.
> Click the [ADD A NEW OFFLINE PARAMETER] button.
> The ADD OFFLINE PARAMETER pop-up screen appears.
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> Select an appropriate offline parameter from the list and click the
[OK] button at the bottom of the pop-up screen.
> The new offline parameter is added to the offline parameter list.
To configure a completely new offline parameter proceed as follows:
> Click the [CREATE] button in the ADD OFFLINE PARAMETER popup
screen.
> A new pop-up screen with the configuration of the offline parameter
appears.
> Enter any name for the offline parameter (mandatory).
> Select a parameter type for the offline parameter (optional).
> Select the units for the offline parameter (optional).
> Define the decimals for the offline parameter (mandatory).
> Click the [SAVE] button in order to save your settings.
Configuring an offline parameter can be helpful if you
already know what kind of samples you will take during
the cultivation process. For example the definition
of biomass (xt)- and substrate concentration (st) may
be useful for visualising the taken offline samples in
the chart display.
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Soft-sensor:
If no appropriate soft-sensor is listed you have to configure
a new soft-sensor in the library (see "Softsensors"
on page 131)
To select a soft-sensor for your batch proceed as follows:
> Click the [SOFT SENSOR PARAMETERS] button in the CONFIGURE
PARAMETERS section.
> Click the [ADD] button at the top of the soft-sensor parameters list.
> A new window select a soft-sensor appears.
> Click on the soft-sensor from your soft-sensor library.
> Click the edit button to change the constant values if necessary.
> Click the [OK]button to save your selected soft-sensorparameter.
> The selected soft-sensors are displayed in the parameter list.
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4.1.1.4 Device Selection
> Click the icon in the SELECT DEVICE field in order to select the
appropriate device for your batch process.
> The CHANGE DEVICE pop-up screen appears, where all your configured
devices are listed.
> Click on any free vessel in order to continue with the configuration
of the batch process.
> Click the [OK] button at the bottom of the CHANGE DEVICE popup
screen in order to save your settings and get back to the BASIC
SETUP screen.
If you simply want to plan an experiment it is also possible
to select a bioreactor vessel which is in use.
However, to start a batch process the device must be
free.
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4.1.2 Organism information
The organism information allows you to specify further details about
the organism of the specific batch process. This information is
optional. However, the information can be connected/linked to several
other applications and allows easy storage of batch information
in one place.
Configuring the organism information, following conditions have to
be met:
– The BASIC SETUP of the batch has to be specified (see "Basic
setup" on page 27)
– The allocation of a specific device (see "Device Selection" on the
previous page)
– Optional: Configuration of the process parameters (see "Parameter
configuration" on page 29)
In order to access the organism information proceed as follows:
> Click the [SINGLE BATCH] button on the left-hand side of the
PLAN & RUN menu.
> Click on the ORGANISM icon in the work flow illustration or on the
[NEXT] button at the bottom of the screen.
> The ORGANISM screen appears.
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Number Meaning
1 Current work flow position (ORGANISM)
2 Specification of organism group
3 Specification of organism
4 Specification of clone/strain
5 ID Number
6 Inocoulum volume at inoculation time point
7 Inoculum concentration/OD at inoculation time point
Inoculum volume and inoculum concentration/OD can
also be specified during a running batch process as
soon as the batch is inoculated (see "Inoculate batch
process" on page 102).
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4.1.2.1 Select organism
If no organism is selectable you have to create a organism
in the library (see "Add new organism" on
page 120).
> Click the icon in the ORGANISM CLASS section to select the
appropriate organism for your batch process.
> The select organism pop-up screen appears.
> Click on the appropriate organism for your batch process.
> Click the [OK] button in order to save your settings.
> Information about the specific organism is displayed in the organism
screen.
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4.1.2.2 Inoculum information
Inoculum volume:
> Enter any value in the INOCULUM VOLUME field for your
inoculum volume.
> Select the corresponding unit for your inoculum volume in the drop
down menu next to it.
> The inoculum volume with the corresponding unit is displayed.
Inoculum concentration:
> Enter any value in the INOCULUM CONCENTRATION field for
your inoculum concentration.
> Select the corresponding unit for your inoculum concentration in
the drop down menu next to it.
> The inoculum concentration with the corresponding unit is dis-
played.
Specifying the inoculum concentration ( x 0 ) as an
example enables the possibility to use this value for a
biomass growth model. Furthermore, any entered
information in this section is saved to the batch process
and always accessible.
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4.1.3 Culture media information
The culture media information allows you to specify further details
about the culture media of the specific batch process. This information
is optional. However, the information can be connected/linked
to several other applications and allows easy storage of batch information
in one place.
Configuring the culture media, the following conditions have to be
met:
– The basic setup of the batch has to be specified (see "Basic setup"
on page 27)
– The allocation of a specific device (see "Device Selection" on
page 35)
– Optional: Configuration of the process parameters (see "Parameter
configuration" on page 29)
– Optional: Configuration of the organism information (see "Organism
information" on page 36)
In order to access the culture media information proceed as follows:
> Click the [SINGLE BATCH] button on the left-hand side of the
PLAN & RUN menu.
> Click on the CULTURE MEDIA icon in the work flow illustration or
on the [NEXT] button at the bottom of the screen.
> The CULTURE MEDIA screen appears.
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Number Meaning
1 Current work flow position (CULTURE MEDIA)
2 Selection of stored culture media
3 Culture media entries
4 Edit specific culture media entry
5 Delete specific culture media entry
4.1.3.1 Culture media selection
> Click the [ADD CULTURE MEDIA] button at the top of the culture
media screen.
> The SELECT A CULTURE MEDIA pop-up screen appears.
> Select the appropriate culture media for your batch process.
> As soon as you click on a culture medium the list of the compounds
appears.
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> Click the [OK] button in order to save your selection.
If no culture media is selectable you have to create a
culture media in the library (see "Add new culture
medium" on page 124).
4.1.3.2 Edit culture media compounds
Simple changes of the culture media compounds can be conducted
by clicking into the corresponding fields. Each amount and unit of a
specific culture media compound can be changed directly in the displayed
list.
> Click directly on the specific culture media compound which you
want to change and enter the new value.
> Change the unit of the specific culture media compound.
> The changed value will turn immediately into orange as an indication
of change.
By clicking on the icon you can easily delete a culture
medium compound from the current culture
media compound list.
Add a culture media compound:
> Click into the enter a compound field at the top of the culture
media compound list.
> Enter the new culture media compound.
As you start to type any culture media compound a list
of proposed culture media compounds (synchronised
from the culture media compounds library) appears
and allows you to select you desired culture media
compound.
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Save changes of the selected culture media:
Changing any values or compounds of the selected culture media results
into two options for saving the altered culture medium:
– Save the altered culture medium to the culture media library
– Save the altered culture medium to the current batch process
> Click the [SAVE TO LIBRARY] button to save the altered culture
media to the culture media library.
> The save to library work flow steps appears.
> Enter a new name for the altered culture media.
> Click the [SAVE] button in order to save the altered culture
medium in the culture media library and continue in the PLAN &
RUN work flow.
Click the [OK] button if you simply want to save the
altered culture medium to your batch process and continue
in the PLAN & RUN work flow.
If you do not save the altered culture media to your culture
media library the culture media will not be available
for any further procedures.
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4.1.4 Batch strategy
The BATCH STRATEGY section is the source of every advanced
batch process strategy configuration. Multiple phases with specific
transitions and different parameter set points are easy to configure.
In addition, customised functions or scripts for any complex strategy
can be applied as required.
Configuring the batch strategy, the following conditions have to be
met:
– The setup of the batch has to be specified (see "Basic setup" on
page 27)
– The allocation of a specific device (see "Device Selection" on
page 35)
– Optional: Configuration of the process parameters (see "Parameter
configuration" on page 29)
– Optional: Configuration of the organism information (see "Organism
information" on page 36)
– Optional: Configuration of the culture media information (see "Culture
media information" on page 40)
In order to access the batch strategy proceed as follows:
> Click the [SINGLE BATCH] button on the left-hand side of the
PLAN & RUN menu.
> Click on the BATCH STRATEGY icon in the work flow illustration or
on the [NEXT] button at the bottom of the screen.
> The strategy screen appears.
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Number Meaning
1 List of all configured process parameters
2 Name of the phase
3 Icon for deleting a phase
4 Icon for transition rule between several phases
5 Visualisation of a configured function
6 Current work flow position
7 Icon for adding a phase
For a simple fed-batch cultivation the preprogrammed
function "exponential" can be used to get a exponential
feeding profile. The exponential feed profile is
based on Ft = F0 ∙ eµ∙t where F0 and µ∙t have to be
defined.
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4.1.4.1 Phase configuration
Each batch process can consists of several phases. Defining the number
of phases at the very beginning helps to build the frame work of
the strategy. As soon as all the phases are configured it is easy to configure
each parameter for every phase. In each phase several process
parameters can be controlled by three types:
– Fixed values (e.g. Temperature 30 °C)
– Functions (e.g. exponential feed rate)
– Enabled (on) / Disabled (off) (e.g. activation of soft-sensor)
By default every batch process starts with the phases
"Preparation" and "Batch". The transition for this two
phases is triggered by inoculating the batch (see
"Inoculate batch process" on page 102). However, the
two preconfigured phases can be renamed or deleted.
Add a phase:
> Click the [PLUS] button on the right-hand side of the last phase con-
tainer.
> A new phase is attached to your strategy and connected by a
unconfigured transition.
> Click into the name of the phase and enter a new name.
> The new phase is ready for the configuration of all process para-
meters.
Delete a phase:
> Click the icon on the right-hand side of the phase container.
> Confirm the deletion of the selected phase.
> The entire phase is deleted immediately.
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4.1.4.2 Transition rules
Transition rules define in which circumstances the strategy continues
and the next phase gets active. Each transition rule operates as a trigger
and starts the strategy for every phase.
The following five different options are available:
– Parameter - value rule (see "Parameter - value rule:" on the next
page)
– Parameter - parameter rule (see "Parameter - Parameter rule:" on
page 49)
– Event rule (see "Event rule:" on page 50)
– Time rule (see "Time-out rule:" on page 50)
– Combining of several transition rules (see "Combining transition
rules:" on page 52)
> Add a new phase to the strategy.
> Click the orange transition icon between two phases.
> The TRANSITION RULES screen appears.
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Parameter - value rule:
The parameter - value rule is the standard transition rule where any
defined parameter reaches a certain value and triggers the transition
rule.
> Click the [PARAMETER - VALUE RULE] button at the top of the
TRANSITION RULE screen.
> A new rule is added to the list with a drop down list of all parameters,
a boolean expression and a value field.
> Select any specific parameter from the drop down list.
> Select any appropriate boolean expression from the drop down list.
> Enter any value for the transition rule in the corresponding field.
> Click the [OK] button in order to save your settings.
If you want to delete a transition rule simply click on
the icon on the right-hand side of the transition rule.
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Parameter - Parameter rule:
The parameter - parameter rule can be used to compare calculated
and measured parameters (e.g. off-line and on-line values) or any
other similar parameters.
> Click the [PARAMETER - PARAMETER RULE] button at the top of
the TRANSITION RULE screen.
> A new rule is added to the list with a drop down list of all parameters,
a boolean expression and a value field.
> Select any specific parameter from the drop down list.
> Select any appropriate boolean expression from the drop down list.
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> Select any corresponding parameter from the drop down list.
> Click the [OK] button to save your settings.
Event rule:
The event rule is based on any event during the batch process and
can be used to trigger the transition rule by this specific event (e.g.
inoculation).
> Click the [EVENT RULE] button at the top of the TRANSITION RULE
screen.
> A new rule is added to the list with a drop down list of all parameters,
a boolean expression and a value field.
> Select any process event from the drop down list.
> Click the [OK] button in order to save your settings.
Time-out rule:
The time-out rule can be used to define the duration of a specific
phase.
> Click the [TIMEOUT RULE] button at the top of the TRANSITION
RULE screen.
> A new rule is added to the list with a drop down list of all parameters,
a boolean expression and a value field.
> Enter any value for the duration of the phase in the corresponding
field.
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> Select any appropriate time unit from the drop down list.
> Click the [OK] button in order to save your settings.
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Combining transition rules:
All of the four transition rules can be combined. A specific transition
rule also can be used multiple times (e.g. 3 times parameter - value
rule).
> Click on any of the four transition rule buttons.
> An additional transition rule appears in the transition rule screen.
> Select one of the boolean connection buttons.
> Click the [OK] button in order to save your settings.
Several transition rules can only be combined by one of
the boolean connection buttons (e.g. 3 different transitions
rules are connected with "AND").
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4.1.4.3 Parameter set points
Defining different kind of phases allows you to change the set point
of a parameter at a specific time point during the process strategy.
> Click in any parameter entry field in order to change the specific
set point.
> Enter a new value for the specific parameter set point.
The set point of a parameter continues as long as no
other value/function is configured for this specific parameter
(i.e. also for several process phases). This rule is
also true for any configured function which reaches a
certain maximum/minimum at a specific time point. As
long as no other specific set point is configured in the
strategy the parameter value will not change.
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4.1.4.4 Predefined functions
For each parameter any function can be configured within a specific
process phase or for several process phases. eve provides a set of predefined
functions which can be applied to any parameter. The following
predefined functions are available:
– Linear (see "Linear function:" below)
– Ramp (see "Ramp function:" on page 56)
– Exponential (see "Exponential function:" on page 57)
– Step (see "Step function:" on page 58)
– Gravimetric Feed (see "Gravimetric Feed:" on page 60)
> Click the icon on the right-hand side of the parameter entry
field in order to configure any function for a specific parameter.
> The CREATE FUNCTION screen appears.
> Select for how many phases the specific function should be applied
(e.g. preparation- and batch-phase or a single phase).
> Select any of the provided functions and click on the corresponding
icon.
> The FUNCTION CONFIGURATION screen appears where - depending
on the function - several constants can be defined
Linear function:
The preconfigured linear function consists of the two constants
namely, the slope and the offset (intercept). For each constant any
real number can be configured.
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> Click the linear function icon in order to configure the linear func-
tion.
> The linear function configuration screen appears.
> Enter any real numbers for the constants slope (a) and offset (b).
> Click the [OK] button in order to save your settings.
If the configured function does not reach the planned
end value (maximum/minimum) within the configured
phase (see "Transition rules" on page 47) the set point
of this specific parameter will no longer follow the configured
function and the last value will be applied.
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Ramp function:
The preconfigured ramp function is based on the linear function but
consists of the start and the end point and any specified time interval.
This function is especially applicable when no previous information
(i.e. calculation and models) of the parameter is available.
> Click the ramp function icon in order to configure the ramp func-
tion.
> The ramp function configuration screen appears.
> Enter any real number for the start and end set point.
> Enter any real number for the time interval and select the corresponding
time unit.
> A live visualisation of the configured function is displayed.
> Click the [OK] button in order to save your settings
The time interval defines the slope of the ramp function
and by that how fast the end set point is reached.
If the configured function does not reach the planned
end value (maximum/minimum) within the configured
phase (see "Transition rules" on page 47) the set point
of this specific parameter will no longer follow the configured
function and the last value will be applied.
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Exponential function:
The preconfigured exponential function consists of two constants
namely, the factor and the exponent. For each constant, any real
number can be configured.
> Click the exponential function icon in order to configure the exponential
function.
> The exponential function configuration screen appears.
> Enter any real number for factor a (F0) and the exponent b (µ).
> A live visualisation of the configured function is displayed.
> Click the [OK] button in order to save your settings.
If the configured function does not reach the planned
end value (maximum/minimum) within the configured
phase (see "Transition rules" on page 47) the set point
of this specific parameter will no longer follow the configured
function and the last value will be applied.
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Step function:
The step function is a table that allows to configure a certain parameter
value as setpoint for any given timepoint (absolute).
> Click the step function icon in order to configure the step function.
> The step function configuration screen appears.
> Click the [ADD STEP] button in order to add any additional step to
your table.
> Within a step configure the specific (absolute) timepoint (hh:mm:ss)
when the setpoint change should be applied.
> The table is always ordered automatically as soon as you enter a
new value.
> Click into the setpoint entry field in order to confgure any setpoint
of the parameter for this specific step.
> If necessary, you can click the repeat checkbox in order to repeat
the entire step function (all steps).
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The repeat function within the step function is especially
useful when a repetitive profile is applied.
If the configured function does not reach the planned
end value (maximum/minimum) within the configured
phase (see "Transition rules" on page 47) the set point
of this specific parameter will no longer follow the configured
function and the last value will be applied.
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Gravimetric Feed:
Gravimetric Feed is a function designed for feed pumps (although
available for all parameter types). The evolution of the setpoint will
follow a feed rate profile in g h-1 based on the flow rate of the pump
and controlled by the weight of a specific balance.
Two types of the gravimetric feed are available, namely:
– Feed rate based: If this mode is selected, the feed is optimised for
feed rate. The overall (absolute) weight can drift due to interferences
on the balance.
– Weight based: If this mode is selected, the feed is optimised for
feed integral. The feed rate will fluctuate more.
> Click the step function icon in order to configure the step function.
> The step function configuration screen appears.
> Select the type of gravimetric feed control by clicking on the [FEED
RATE] or [WEIGHT] button.
> Select the connected balance for your gravimetric feed.
In the drop down selection all parameters of type
"Weight" and "Unknown" are displayed and select-
able.
> Enter the maximal flow rate of the used pump at 100% (Infors
HT equipment provides a built in calibration function for that) in
g h-1.
> Enter the values for factor a (F0) and the exponent b (µ).
> Click the [OK] button in order to save your settings.
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4.2 Multiple Batch
The MULTIPLE BATCH is a function which allows you to plan and run
batch processes in parallel. The entire work flow is almost the same
as for a single batch process (four work flow steps). However, at the
beginning of a multiple batch process a reference batch has to be
configured which defines a rough frame work for all the batch pro-
cesses.
4.2.1 Reference batch configuration
The reference batch is the source configuration of every multiple
batch experiment and will be applied automatically to all batch processes
within the specific experiment.
> Click the [MULTIPLE BATCH] button on the left-hand side of the
PLAN & RUN menu.
> A generic setup screen for multiple batch experiment appears.
> Enter any name for the multiple batch experiment.
The name of the multiple batch experiment will be
used both, for the experiment and the single batch processes
within the experiment. Each batch process will
have an ascending number next to the batch name.
> Enter any description about the multiple batch experiment.
> Follow the instructions of single batch for the project allocation (see
"Project allocation" on page 28).
> Enter the amount of batch processes within the multiple batch
experiment.
> Click the [CONFIGURE REFERENCE BATCH] button at the bottom
right-hand side of the screen
> The basic setup screen with the entered experiment name and
description appears.
> Follow the instructions of single batch for the device selection (see
"Device Selection" on page 35)
> Follow the instruction of single batch for the parameter configuration
(see "Parameter configuration" on page 29).
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> Follow the instruction of single batch for the organism information
(see "Organism information" on page 36)
> Follow the instruction of single batch for the culture media selection
(see "Culture media selection" on page 41)
> Follow the instruction of single batch for the strategy configuration
(see "Batch strategy" on page 44).
> Click the [GENERATE EXPERIMENT] button at the bottom righthand
side of the strategy screen.
> The multiple batch overview table of all the planned batch processes
appears.
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4.2.2 Multiple batch overview table
The reference batch is applied to the number of defined batch processes
within the multiple batch experiment. As soon as the experiment
is generated the following multiple batch overview table
appears:
Number Function
1 Name of the multiple batch experiment
2
Name of the single batch processes where each
batch process gets a individual number
3a, 3b Button for selecting equipment, change basic setup
4a, 4b Organism description, change organism information
5a, 5b
Culture media description, change culture media
information
6a, 6b Strategy description, change strategy
7 Start button for single batch process
8
Delete button in order to delete a single batch process
from the overview table
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4.2.2.1 Change device
In order to change the device for a single batch process proceed as
follows:
> Click on the device name button in the basic setup column of the
multiple batch overview table.
> The CHANGE DEVICE pop up screen appears.
> The vessels of your devices are listed (in use/free) and can be selec-
ted.
> Click on any free vessel of your devices.
> Click the [OK] button in order to save your settings and get back to
the multiple batch overview table.
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4.2.2.2 Change basic setup
In order to change the basic setup for a single batch process proceed
as follows:
> Click the icon in the basic setup column of the multiple batch
overview table.
> The BASIC SETUP screen of this specific batch process appears.
> Follow the instruction of single batch for edit the basic setup (see
"Basic setup" on page 27)
> Click the [BACK TO OVERVIEW] button to get back to the multiple
batch overview table.
If you want to change the name of a single batch process
you can click directly into the name column of the
multiple batch overview table and change it.
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4.2.2.3 Change organism information
In order to change the organism information for a single batch process
proceed as follows:
> Click the icon in the organism column of the multiple batch
overview table.
> The ORGANISM screen of this specific batch process appears.
> Change the organism by clicking the icon next to the change
organism option.
> The SELECT ORGANISM pop up screen appears.
> Click on the appropriate organism for your single batch process.
> Click the [OK] button in order to save your settings.
> Click the [BACK TO OVERVIEW] button to get back to the multiple
batch overview table.
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4.2.2.4 Change culture media information
In order to change the culture media information for a single batch
process proceed as follows:
> Click the icon in the culture media column of the multiple batch
overview table.
> The CULTURE MEDIA screen of this specific batch process
appears.
> Click the [ADD CULTURE MEDIA] button on the top of the
CULTURE MEDIA screen.
If you want to delete or edit an existing culture media
click the or icon on the right-hand side of the culture
media list.
> The ADD CULTURE MEDIA pop-up screen appears.
> Follow the instruction of single batch for edit the culture media (see
"Edit culture media compounds" on page 42).
> Click the [OK] button in order to save your settings.
> Click the back to overview button to get back to the multiple batch
overview table.
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4.2.2.5 Change strategy
In order to change the strategy for single batch process proceed as fol-
lows:
> Click the icon in the strategy column of the multiple batch overview
table.
> The STRATEGY screen of this specific batch process appears.
> Follow the instructions of single batch to edit the strategy (see
"Batch strategy" on page 44).
> Click the [BACK TO OVERVIEW] button to get back to the multiple
batch overview table.
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4.2.2.6 Start, open or delete a batch process
The multiple batch overview table allows three different actions for a
single batch process:
– Start batch process (see "Start single batch process:" below)
– Open batch process (see "Open single batch process:" on the next
page)
– Delete batch process (see "Delete single batch process:" on
page 71)
Start single batch process:
> Click the [START] button in the action column of multiple batch
overview table.
> The MONITOR screen of this specific batch process appears.
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Open single batch process:
> Click the [OPEN] button in the action column of multiple batch
overview table.
> The MONITOR screen of the specific batch process appears.
At the top of the chart the name of the multiple batch
experiment is displayed. Click on it to get back to the
multiple batch overview table. In addition, the running/started
batch processes within the experiment are
listed in a tab which lets you switch between several
batch processes quickly.
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Delete single batch process:
> Click the [DELETE] button on the right-hand side of the action
column in the multiple batch overview table.
> Confirm that you really want to delete the specific batch process
from the multiple batch overview table.
> The new list (updated) of all the batch processes within the multiple
batch experiment appears.
4.2.2.7 Custom functions and scripts
The custom parameter functions and scripts allows you to program
any function or script for parameters or advanced customised control
strategies. The scripts are based on the C# (C sharp) scripting language
and are exactly the same as for soft-sensors (see "Edit a softsensor"
on page 142).
> Click the custom function icon in order to configure the custom
function.
> The custom function configuration screen appears.
> Enter any function or script in the editor window.
Sine function example:
//Setpoint as a Sine
var startValue = (output.MaximalValue - output.MinimalValue)
/ 2.0;
var sineRange = startValue * 0.7;
output.Setpoint = startValue + sine Range * Math.Sin
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> An automatic syntax checker will ensure the correct syntax. Syntax
errors will be notified and displayed with an error message in the
corresponding script line.
> Click the [OK] button in order to save your settings.
If the configured function does not reach the planned
end value (maximum/minimum) within the configured
phase (see "Transition rules" on page 47) the set point
of this specific parameter will no longer follow the configured
function and the last value will be applied.
Fore a more comprehensive description about the
scripting in eve®, please ask for the eve® scripting
guide.
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5 Monitor & Analyse
In the MONITOR & ANALYSE menu, all your running and completed
batch processes can be monitored and analysed. The monitor section
allows you to configure the measured values from the batch process,
displaying and structuring them in a convenient way.
This chapter covers the following topics:
– Open and monitor a batch process (see " Monitor a batch process"
on the facing page)
– Chart configuration (see "Chart configuration" on page 78)
– Add new charts and general chart management (see "Chart windows
management" on page 91)
– Save and load chart templates (see "Save a chart template" on
page 93)
– Print and save charts (see "Chart export" on page 95)
– Batch comparison of live and historical data (see "Batch comparison"
on page 96)
– Operator-mediated batch adjustments (see "Operator-mediated
batch adjustments" on page 101)
In order to monitor and analyse a batch process proceed as follows:
> Click the [MONITOR & ANALYSE] button on the left-hand side of the
screen.
> The sub-menu of MONITOR & ANALYSE appears.
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5.1 Monitor a batch process
In order to monitor and display a batch process, different ways to
open the chart display are available, depending on the status of the
selected batch process.
Running batch process:
> Click the [BATCHES] button on the left-hand side of the MONITOR
& ANALYSE menu.
> The BATCHES screen appears with all the running and completed
batch processes.
> Click the [OPEN] button on the right-hand side of the batches list.
> The chart display of the specific batch process appears.
In addition, every running experiment and its batch processes can be
accessed through the dashboard:
> Click the [DASHBOARD] button on the left-hand side of the menu.
> The DASHBOARD screen appears with the running batches and
planned experiments.
> Click either directly on the experiment name (link) or on the
[OPEN] button on the right-hand side of the batch process.
> The chart display of the specific batch process appears.
Completed batch processes:
> Click the [LIBRARIES] button on the left-hand side of the menu.
> Click the [BATCHES] button in the sub-menu selection.
> The BATCHES screen appears with all running and completed
experiments and batch processes.
> Click the [OPEN] button on the right-hand side of the experiment
list.
> The chart display of the specific batch appears.
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5.1.1 Alarms
Alarms can be configured for every parameter. Each parameter
alarm can have a defined range with fixed or dynamic limits (see
"Parameter configuration" on page 29). When a parameter value is
out of range the alarm signal turns red.
The visual notification of an alarm is a tab at the bottom (centre) of
the window in each menu/sub-menu. Depending on the alarm state
the alarm tab is flashing in red or appears in white.
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To see and expand the list of the current alarms, proceed as follows:
> Click the [ALARMS] button at the bottom of the window (either in
red or white).
> The alarm list appears.
Number Function
1 Direct link to Audit trail
2 Active alarm (parameter is out of range)
3 Inactive alarm (parameter was out of range)
4 Open batch with chart
5 Acknowledge alarm (only applicable for inactives)
5.1.1.1 Subscribe to e-mail alarm notifications
All running batches can be monitored additionally by e-mail alarm
notifications (see "Settings" on page 169) . In the MONITOR &
ANALYSE batch library each user can subscribe (receiving e-mail
alarm notifications) to certain batches.
To subscribe to a batch, proceed as follows:
> Click on the toggle button in the E-MAIL ALARM column of a specific
batch.
> You are subscribed/unsubscibed to the specific batch
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E-Mail alarm notifications will be sent only once when
a parameter is out of range.
5.1.1.2 Active alarm
An alarm is active when the current value of the parameter is out of
range. The user cannot acknowledge the alarm but only open the
batch by clicking the icon. When an alarm is active, the entry
itself and the alarm notification tab is red.
Parameters which are out of range are both, shown in
the alarm tab list and the audit trail (see "Audit trail"
on page 144).
5.1.1.3 Inactive alarm
An alarm is inactive when the current value of the parameter has
been out of range but is within the range again. The user can
acknowledge the alarm by clicking the icon. When an alarm is
inactive, the entry itself is green but the notification tab is red.
Parameters which has been out of range are both,
shown in the alarm tab list and the audit trail (see
"Audit trail" on page 144).
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5.2 Chart configuration
Number Meaning
1 Tab of current batch / compare view
2
Edit mode to switch between batch time and inoculation
time
3 Tool tip
4 Export function
5
Chart settings: axis configuration, templates, refresh
rate and delete chart
6 Zoom and navigation window
7 x (time) axis
8 y axis
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5.2.1 Axis configuration
By default all the parameters are displayed on the first left axis (L1).
In order to change the appearance of the parameters proceed as fol-
lows:
> Click the icon on the right-hand side of the chart display.
> A menu with setting options for the chart display appears.
> Click the CONFIGURE AXIS option.
> The CONFIGURE AXIS pop-up screen appears.
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5.2.1.1 Change parameter position
In order to change the position of a specific parameter, proceed as
follows:
> Click on any number in order to allocate your parameter to one of
the six axes (left 1-3 and right 1-3).
> The specific parameter appears on the selected axis (second left in
this case) on the chart display.
> Click on the dash in order to hide a parameter.
> The specific parameter disappears from the chart display.
If two or more parameters have a similar axis scale it is also possible
to combine them on one axis:
> Click on the same number in order to allocate your two parameters
to the same axis.
> Both of the parameters appear on the same axis on the chart dis-
play.
As soon as you allocate a parameter to a specific axis
the parameter will appear on the selected axis (live synchronisation
in the background on the chart display).
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5.2.1.2 Show and hide all parameters
Hide all parameters:
> Click the [HIDE ALL PARAMETERS] button at the top of the AXIS
PARAMETERS pop-up screen.
> All the parameters disappear from the chart display.
Show all parameters:
> Click the [SHOWALL PARAMETERS] button at the top of the AXIS
PARAMETERS pop-up screen.
> All the parameters appear on the chart display on the first left axis.
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5.2.2 Parameter appearance
The appearance of each parameter can be customised, completely.
In order to configure the appearance of a specific parameter, proceed
as follows:
> Click directly on the axis caption (parameter name) either on the
left-hand side or right-hand side of the main chart display.
> The PARAMETER APPEARANCE pop-up screen appears.
5.2.2.1 Change axis
> Click on any number in the AXIS option in order to allocate your
parameter to one of the six axes (left 1-3 and right 1-3).
> The specific parameter appears on the selected axis (first left in this
case).
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5.2.2.2 Change marker
> Click on the icon in the MARKER option to change the marker
of your parameter appearance.
> A drop down list with different markers appears.
> Select a new marker for the specific parameter.
> The specific parameter appears with the new marker.
5.2.2.3 Change color
> Click on the icon in the COLOR option in order to change the
color of your parameter appearance.
> A drop down list with different colors appears.
> Select a new color for the specific parameter.
> The specific parameter appears with the new color.
5.2.2.4 Change line size
> Click on the cursor of the scroll bar in the LINE SIZE option and
move it to the right (thicker) or to the left (thinner) in order to
change the line size.
> The specific parameter appears with the new line size.
Any change in parameter appearance has immediate
effect and is visualised live in the background on the
chart display.
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5.2.3 Axis scale
Each axis scale can be configured individually. In order to change the
axis scale proceed as follows:
> Click on any number on the axis in order to change the scale of the
axis.
> The AXIS SCALE pop-up screen appears.
5.2.3.1 Change min. and max. value
By default all the axis have the AUTO-SCALE option activated. In
order to change the axis scale you have to uncheck this option.
> Click on the auto-scale check box in the AUTO SCALE option.
> The entry field for min. and max. value is activated.
> Enter a new value for the min. and max. value.
> Click the [SET] button at the bottom of the pop-up screen.
> The configured axis scale appears on the chart display.
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5.2.3.2 Change decimals of the axis scale:
> Click on the cursor of the scroll bar in the DECIMALS option and
move it to the right or to the left in order to change the amount of
decimals.
> Click the [SET] button at the bottom of the pop-up screen.
> The changed decimals of the axis scale appears on the chart dis-
play.
Each parameter has a set number of decimals. In order
to change the decimals for the entire system you have
to configure the specific device parameter (see "Edit
device" on page 156).
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5.2.4 Time scale and zoom
Depending on how long a batch process is running, either batch time
in hours or a date/time format time scale may be more convenient.
In addition, enlargement of a specific area can help to differentiate a
behaviour of a specific parameter.
5.2.4.1 Change time scale
> Click directly on a time point on the x-axis in order to change the
time format.
> The scale of the x-axis changes.
– An example of a date/time format time scale.
– An example of a batch time in hours.
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5.2.4.2 Change time since inoculation
During the monitoring the chart can be configured that the hours
before the inoculation are negative and the zero point shifts towards
the incoulation timepoint:
> Click the icon to switch from total batch time to batch time since
inoculation.
> The batch time (since inoc.) is displayed next to the clock icon ( )
as well as on the axis itself (zero point is moved towards the inoculation
line).
5.2.4.3 Zoom navigation:
> Click (and hold) anywhere on the chart display and drag the mouse
pointer to the time point where you want to end the zoom.
> The area where the zoom is applied to gets a pale blue back-
ground.
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Navigation of the zoom window:
At the bottom of the chart display a quick navigation toolbar is available.
As soon as any zoom is applied to an area on the chart display
the quick navigation toolbar can be used for navigation. In order to
scroll on the time scale with the selected zoom window, proceed as
follows:
> Drag on the edges of the pale blue zoom window at the bottom of
the chart display or click directly into the area an drag it to a specific
time point.
> The selected zoom area is applied to the chart display.
Reset the zoom:
> Click on the [FULL] button at the top left-hand side of the chart dis-
play.
> The chart display immediately shows the full screen mode.
> Click the 1 hour - or 1 day - zoom button in order to zoom exactly 1
hour or 1 day.
The very top left of the chart displays if the data is
"Live" (running process) or "History" (past).
5.2.5 Tool tip mode
Two different tool tip modes are available. In order to change the
tool tip mode proceed as follows:
> Click the icon on the right-hand side of the chart display.
> A menu with setting options for the chart display appears.
> Click the CHANGE TOOL TIP MODE option.
> The appearance of the tool tip mode changes.
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– Example of the default tool tip mode.
– Example of the second tool tip mode in the upper left corner of the
chart display.
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5.2.6 Refresh rate
The refresh rate is a setting which defines how often the recorded
parameters are refreshed on the chart display.
> Click the icon on the right-hand side of the chart display.
> A menu with setting options for the chart display appears.
> Click the SET REFRESH RATE option.
> The REFRESH RATE POP-UP screen appears.
> Enter a new value for the refresh rate.
> Click the [OK] button to save your settings.
> The newly entered refresh rate is set to the chart display.
Depending on if the "apply to all" option is active or
not, the refresh rate is applied to a single chart display
or to all the chart displays on the monitor. Furthermore,
the refresh rate is always saved in the templates.
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5.3 Chart windows management
The chart display screen is capable of visualising up to 4 charts. Each
of the charts can be configured, positioned and scaled individually.
Number Meaning
1 Add chart button to position and scale the charts
2 First chart display with one parameter
3 Second chart display with one parameter
4 Third chart display with several parameters
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5.3.1 Add additional charts
> Click the [ADD CHART] button on the top right-hand side of the
chart display.
> A new chart is added and the chart display appears in split screen.
You can add up to four charts by clicking on the [ADD
CHART] button.
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5.4 Chart templates
The entire configuration of a chart display can be saved as a template.
The saved templates are stored (user specific) and always
accessible for the current user. In order to save a template of the
chart configuration proceed as follows:
> Click the icon on the right-hand side of the chart display.
> A menu with setting options for the chart display appears.
> Click the SAVE AND LOAD TEMPLATE option
> The template pop-up screen appears.
5.4.1 Save a chart template
> Click the [SAVE CURRENT CONFIGURATION AS TEMPLATE] button
in the template pop-up screen.
> A new input entry field appears where the name and a description
of the template can be specified.
> Enter any name for the template.
> Enter any description for the template (optional).
> Click the [OK] button in order to save the template.
> The template is saved and can now be used.
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5.4.2 Apply a chart template
> Click the [APPLY] button on the right-hand side of the template
pop-up screen.
> The selected template is applied to the specific chart display.
5.4.3 Delete a chart template
> Click the [DELETE] button on the right-hand side of the template
pop-up screen.
> Confirm the deletion of the selected template.
> The selected template is deleted.
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5.5 Chart export
Each chart display can be printed or exported as a pdf (print) or raw
csv format.
Number Function
1 Icon for export settings
2
Option for print chart display and/or download as
pdf file
3 Option to export raw data as csv
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5.5.1 Print chart
> Click the icon on the right-hand side of the chart display.
> A menu with export options for the chart display appears.
> Click the PRINT CHART option.
> The print option screen of your running system appears.
> Click the [PRINT] button on the left-hand side of the print screen.
> The chart display is printed.
Depending on the operating system installed, the
standard print option screen may look different.
5.6 Batch comparison
The batch comparison function is a very powerful tool to compare
several batch processes independent of the origin of the data i.e. historical
(stored) or live data (running batch). The compare function
can always be accessed from the monitor chart display.
5.6.1 Select batch processes
In order to compare several batch processes proceed as follows:
> Open any batch process either a live running batch process or a
closed batch process from the library (see " Monitor a batch process"
on page 74).
> The chart display of the specific batch process appears.
> Click the [COMPARE BATCHES] button at the bottom right-hand
side of the chart display.
> A new table with all completed and running batch processes
appears.
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> Select the batch processes for the comparison by clicking on the
check box on the left-hand side of the table.
> Click the [COMPARE] button on the bottom right-hand side of the
table.
> A pop-up screen appears where you have to define a new name
for the batch process comparison.
> Enter a new name for the batch process comparison.
> Click the [OK] button.
> The chart display of all the compared batch processes appears.
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5.6.2 Compare batch processes
In order to compare parameters from several batch processes the
same chart display configuration as for a single batch process can be
applied (see "Axis configuration" on page 79).
If the same parameter is compared from several batch processes
each parameter gets a small number in square brackets next to its
parameter name (axis caption) and batch process name in the tabs.
Number Function
1 CO2 axis caption of batch number 3
2 Batch process tab number 3
3 CO2signal of batch number 3
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5.6.3 Synchronise batch processes
In order to overlay parameter signals exactly or to synchronise the
time scale from several batch processes proceed as follows:
> Click the [SYNCHRONISATION] button at the bottom right-hand
side of the compare chart display.
> The SYNCHRONISATION pop-up screen appears.
> If the batch processes are inoculated (see "Inoculate batch process"
on page 102) a synchronisation at the time point where inoculation
happend is also possible.
> Enter the exact time point at the specific batch process where the
synchronisation should be applied to.
> Click the [APPLY] button to see the entire parameter signal move
in the background of the compare chart display.
> Click the [OK] button to save your synchronisation settings.
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5.6.4 Switch between compared batch processes
The compare chart display shows all the compared batch processes
as a tab at the top of the chart display. In order to switch to a single
batch process proceed as follows:
> Click on any tab of the compared batch processes which are listed
as a tab at the top of the chart display.
> The chart display of the select single batch process appears.
> Click on the compare view tab (name of your compare view) to get
back to the compare view (batch_compare in this case).
> The chart display of the compare view appears.
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5.7 Operator-mediated batch adjust-
ments
When a certain batch process is monitored different operator-mediated
batch adjustments can be applied.
Number Meaning
1
Inoculate button for inoculum information and inocu-
lation
2
Offline samples where a measured sample can be
entered with the according time stamp
3
Log book entry where at any time point a comment can
be entered
4 Parameters button to access the list of parameter values
5 Strategy button to access the strategy
6 Skip phase manually button
7 Stop batch button
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5.7.1 Inoculate batch process
> Click the [INOCULATE] button at the bottom of the chart display.
> The INOCULUM pop-up screen appears.
> If available, enter the measured value of your inoculum concentration
into the CONCENTRATION entry field.
The inoculum volume and inoculum concentration
(from the recipe) are pre filled if this information was
provided in the organism work flow (see "Organism
information" on page 36).
> Click the inoculate batch button at the bottom of the INOCULUM
pop-up screen.
> Click the [OK] button next to the [INOCULATE BATCH] button.
> The batch process is inoculated and a red line at the inoculation
time point appears on the chart display.
Once a batch process is inoculated the inoculum
information (i.e. inoculum time point, inoculum
volume and inoculum concentration) can always be
accessed by the [INOCULUM INFORMATION] button
on the bottom of the chart display.
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5.7.2 Offline samples
> Click the offline samples tab on the right hand side of the chart dis-
play.
> The OFFLINE SAMPLES screen appears.
> Click the new [SAMPLE BUTTON] on the top of the OFFLINE
SAMPLES screen.
> The new sample appears in the offline sample list with the corresponding
time stamp.
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> Click the icon on the right hand side of the sample entry in
order to define the offline sample volume.
> Enter any value for the offline sample volume and click to [OK] button
to save your setting
> Click the [ NEW MEASUREMENT] button in order to add any measurement
to this offline sample.
> A new entry field with the configured offline parameter appears.
> Select the appropriate offline parameter from the drop down list.
> Enter any value for the offline parameter measurement and click
the [SUBMIT] button to save your value (termporary).
The submit button allows to save the offline parameter
value in a temporary mode (the value can be edited
with the [EDIT] button). However, to save the offline
parameter permanently you have to confirm the
valvue with the [CONFIRM] button.
> The entered value for the offline parameter measurement is saved
to the offline sample.
> Click the [CONFIRM] button to save the offline parameter value.
By clicking again on the [NEW MEASUREMENT] button
you can add as much measurements as you want
to one single offline sample.
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5.7.3 Logbook entry
> Click the [LOG BOOK] tab on the top right-hand side of the chart
display
> The LOG BOOK screen appears.
> Enter any comment in the message field.
> Click the [SUBMIT] button.
> The entered comment is saved in the log book and appears in the
log book list with the according time stamp.
> Click the LOG BOOK tag again to close the log book.
5.7.4 Parameter set points
> Click the [PARAMETERS] button at the bottom of the chart display.
> A new pop-up window with the list of all recorded parameters
appears.
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> In order to change any parameter set point or parameter alarm proceed
with the steps as for parameter configuration (see "Parameter
configuration" on page 29).
The parameter set point window is a window, which
can be displayed independently on screen to always
have full access to parameter set points.
5.7.5 Change strategy
During a running batch process the entire strategy can be changed.
In order to change the strategy in a running batch process proceed as
follows:
> Click the [STRATEGY] button at the bottom of the chart display.
> A new pop-up window with the strategy screen appears where the
active phase is marked with an orange rectangle.
> In order to change or add a phase, transition or parameter function
follow the procedure in strategy for a single batch (see "Batch
strategy" on page 44).
> Click the save button in order to save your settings.
> The new strategy settings are saved.
> Click the close button in order to get back to the chart display.
5.7.6 Skip phase manually
In addition to time-, event- or parameter-based transition triggers at
the top of the chart display a manual phase trigger button is
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available. In order to skip a phase in the strategy proceed as follows:
> Click the icon at the top of the chart display.
> A confirmation pop-up screen appears.
> Confirm the action by clicking the [OK] button in order to skip the
current phase.
> The next phase is active immediately and displayed in the chart dis-
play.
5.7.7 Stop batch
> Click the [STOP BATCH] button at the bottom of the chart display
> A confirmation pop-up screen appears.
> Confirm the action by clicking the [OK] button in order to stop the
batch process.
> The batch process is stopped and saved in the experiment library.
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6 Libraries
Libraries are a storage place for all different kinds of data and a very
powerful tool to structure and link process information. The library
menu includes the following sub-menus and allows you to store and
prepare any batch process in the appropriate way:
– Import and export batches (see "Batches" on the next page)
– Access recipes (see "Recipe" on page 117)
– Create and edit new organisms (see "Organism" on page 120)
– Create and edit new culture media (see "Culture media" on
page 123)
– Create and edit soft-sensors (see "Soft-sensors" on page 131)
In order to access the libraries proceed as follows:
> Click the [LIBRARIES] button on the left-hand side of the screen.
> All the sub-menus of the libraries appear.
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6.1 Batches
In order to access the library of all planned, closed and running experiment
proceed as follows:
> Click the [BATCHES] button on the left-hand side of the LIBRARIES
menu.
> A list of all planned, running and closed batches appears.
Number Function
1 Open (chart) closed or running batch
2 Export batch button
3 Report batch button
4 Import batch button
5 Advanced filter function
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6.1.1 Archive data
Every completed batch process can be archived. To archive a batch
process, proceed as follows:
> Click the icon on the right-hand side of the experiments library.
> The selected batch is archived.
If you would like to see all batch process inclusive the archived ones,
proceed as follows:
> Select the "All Batches" option at left bottom of the batch library
page.
> The batch library list will show all batch process inclusive the
archived ones.
To unarchive a batch process, proceed as follows:
> Click the icon on the right-hand side of the experiments library.
> The selected batch process in unarchived.
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6.1.2 Import data
The following file formats for the import of any batch data are sup-
ported:
– eve files
– Iris files
– .csv files
– Excel files
In order to import batch data, proceed as follows:
> Click the import button at the bottom of the experiments screen.
> The import batch data pop-up screen appears.
> Click the corresponding import button depending on your file
format.
> A windows browse pop-up screen appears in order to browse your
batch data on the computer.
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> Select your batch data file and click the [OPEN] button at the bottom
of the windows browse pop-up screen.
> Your selected batch data file is saved in the experiment library.
In order to import an excel or .csv file you have to
make sure that this file was created by eve or at least
has the exactly same data structure and order.
6.1.3 Export data
Every batch process can be exported as an eve or .csv file. If a batch
process is not yet completed you can create a recipe out of it (see
"Recipe" on page 117). To export a batch process proceed as fol-
lows:
> Click the icon on the right-hand side of the experiments library.
> The export batch pop-up screen appears.
> Click the corresponding export button depending on what kind of
batch data file you want to create.
> The exported batch data file appears as downloaded file at the bottom
of your web browser.
If you want to display the exported .csv in Microsoft
Excel do not open the .csv file directly but import the
file via the built in Excel import function - otherwise the
format of the displayed data is wrong.
Depending on which web browser you are using, the
download is displayed at the bottom of the web
browser (download folder) or a pop-up appears where
you have to specify the download directory.
6.1.4 Report
Reports can be created both for completed, running and planned
batches and document all relevant batch information in a digital
record (e.g. pdf, ppt etc.) automatically. To create a report of an
experiment (or single batch) proceed as follows:
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> Click the icon on the right-hand side of the experiments library.
> The report batch pop-up screen (COMPONENTS) appears.
If a report is created from a multiple batch experiment
the first step of the work flow starts with "BATCHES".
This step allows to select all the batch processes which
will be included in the report.
Report components:
Report components are defined modules with specific information for
the report (such as organism or culture medium information). By
default all the components are selected and activated. A description
of the components is listed below:
Components Description
ORGANISM Information about the selected organism
CULTURE MEDIA Information about the selected culture media
BATCH STRATEGY Complete description of the batch strategy
LOGBOOKS All logbook entries of the batch
SOFT-SENSORS Complete description of the soft-sensors
BATCH ALARMS All alarms which occurred during the batch
BATCH AUDIT TRAIL All actions (user/device) related to the batch
> Select/deselect your desired components for the report.
> Click the [NEXT] button.
> The PARAMETER matrix appears.
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Report parameters:
For every monitored parameter different parameter specific information
can be displayed. A description of the parameter options is listed
below:
Parameter option Description
SETPOINT
Lists in a table the setpoint configuration of
the parameter
SETTINGS
Lists in a table the alarm configuration of the
parameter
CHARTS
Displays a chart of the parameter with trend,
standard deviation (blue) and min./max.
value (orange).
ALARMS
Lists in a table the alarm events of the para-
meter
AUDIT TRAIL
Lists in a table all the audit trail entries of the
parameter
FUNCTIONS
Lists in a table all relevant information about
the functions of the parameter
DATA POINTS
Allows to display the data points in a averaged
(every 30 data point) or raw (every
second) way
> Select/deselct your desired parameters and parameter options for
the report.
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The selection of the parameters and parameter options
can be executed for every row and column by clicking
on the name of the parameter (e.g. Temp) or parameter
option (SETPOINTS). If you want to deselect or
select all parameters and parameter options click in
the empty cell of the upper left corner.
> Click the [NEXT] button.
> The PREVIEW screen appears.
Report preview:
The preview creates the report. After creation, the report can be
browsed and downloaded (different types of data formats). Depending
on what kind of report components were selected, the report consists
of different parts. However, the following module are included
in every report.
Report module Description
Title page
Title page of the report with: Creation date/time,
creator (user), project name, experiment
name, experiment creation date/time
Index
Index (clickable) of all modules within this
report
Batch overview
Information about: Project, experiment,
batch, state, device, batch time and inoculation
time
Header
Unique identifier of the report on every report
page
To browse through the created report and download it afterwords,
proceed as follows:
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> Click on the small arrows at the top of the preview pop-up screen.
> The next page (index) of the report appears.
> Click the download icon in order to download and save the report
of the experiment/batch.
> Select the appropriate file format (e.g. pdf).
> The report is saved and downloaded and can be open with any program
which can read pdf files.
For default batch report settings please configure the
batch report in global settings (see "Global" on
page 173)
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6.2 Recipe
Recipes are defined configurations of a batch process involving elements
such as equipments, organisms, culture media, soft-sensors,
setpoints and strategies. In order to access the library of created
recipes proceed as follows:
> Click the [RECIPES] button on the left-hand side of the LIBRARIES
menu.
> The recipes library list appears.
6.2.1 Create new recipe
Every batch process can be exported as a recipe. In order to export a
batch process as a recipe proceed as follows:
> Click the icon on the right hand side of the experiments library
(see "Batches" on page 109).
> The export batch pop-up screen appears.
> Click the [RECIPE] button.
> A new pop-up screen appears where you have to enter a name for
the recipe.
> Enter a name for you recipe and click the [SAVE] button.
> The recipe is saved and stored in the recipe library.
6.2.2 Apply recipes
In order to apply a recipe to a specific batch process follow the instruction
for single batch process (see "Load and apply recipe" on
page 28).
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Recipes can also be applied to a reference batch for a
multiple batch experiment and that can save a lot of
planing time.
6.2.3 Import Recipes
Recipes can be imported into eve and applied to any batch process
(see "Load and apply recipe" on page 28) To import a recipe proceed
as follows:
> Click the import button at the bottom of the recipe screen.
> The import recipe pop-up screen appears.
> Define a new name for the recipe and click the [IMPORT] button.
> A windows browse pop-up screen appears in order to browse your
batch data on the computer.
> Select your recipe data file (.json) and click the [OPEN] button at
the bottom of the windows browse pop-up screen.
> Your selected recipe file is saved in the recipe library.
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6.2.4 Export Recipes
Recipes can be exported and shared with other users. An exported
recipe can also be imported at a later time point (see "Import
Recipes" on the previous page). To export a recipe proceed as fol-
lows:
> Click the icon on the right-hand side of the recippe library.
> The recipe is immediately downloaded and saved.
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6.3 Organism
Organisms are defined by the cell type and the clone. The organism
library enables the opportunity to store all relevant information
together about a specific organism and access the data any time.
In order to access the library of created organisms proceed as fol-
lows:
> Click the [ORGANISMS] button on the left-hand side of the
LIBRARIES menu.
> The organism library list appears.
Number Function
1 Add new organism to the library
2 Edit existing organism in the library
3 Delete organism from the library
6.3.1 Add new organism
In order to add a new organism to the organism library proceed as fol-
lows:
> Click the [ADD NEW ORGANISM] button at the top of the organism
library.
> The create organism pop-up screen appears.
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> Enter the corresponding information into the mandatory fields,
indicated by a * after the name.
> Enter the corresponding information into the optional fields.
Pichia pastoris organism example:
Group: Yeast
Name: Pichia pastoris
Clone: GS115
Clone ID: A12
Description: P.pastoris strain for the production of
recombinant inuslin (Mut+)
> Click the [SAVE] button at the bottom of the create organism popup
screen.
> The new organism is added to the organism library list.
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6.3.2 Edit organism
> Click the icon on the right-hand side of an organism entry in the
organism library list.
> The edit organism pop-up screen appears.
> Edit any information of the organism.
> Click the [SAVE] button at the bottom of the edit organism pop-up
screen.
> The changed organism information is saved to the organism and
stored in the organism library list.
6.3.3 Delete an organism
> Click the icon on the right-hand side of an organism entry in the
organism library list.
> Confirm the deletion of the selected organism.
> The selected organism is deleted from the organism library list.
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6.4 Culture media
The culture media library allows to create culture media that can be
used and documented for batches and recipes. In order to access the
library of culture media, proceed as follows:
> Click the [CULTURE MEDIA] button on the left-hand side of the
LIBRARIES menu.
> The culture media library list appears.
Number Function
1 Add new culture media to the library
2 Edit existing culture media in the library
3 Delete culture media from the library
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6.4.1 Add new culture medium
In order to add a new culture medium to the culture media library list
proceed as follows:
> Click the [ADD NEW CULTURE MEDIUM] button at the top of the
culture media library list.
> The CREATE CULTURE MEDIUM pop-up screen appears with a predefined
work flow to create a new culture medium. It starts with
basic details about the culture medium.
> Enter a name for the culture medium (mandatory).
> Enter additional information into the optional fields.
> Click the [NEXT] button to define the compounds of the newly-created
culture medium.
> The predefined work flow continues with the compound list of the
culture medium.
> Click into the enter a compound name or formula field in order to
add a compound to your culture media.
> As soon as you start to type a compound name or compound formula
a selection of your stored compounds is proposed.
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> Click on the chosen compound.
> The compound is added to your culture media compound list.
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> Click into any concentration compound field in order to change the
concentration of the compound.
> Click on any unit compound field in order to change the unit of the
compound.
> Click the [SAVE] button at the bottom of the CREATE CULTURE
MEDIUM pop-up screen.
> The new culture medium is added to the culture media library list.
6.4.2 Edit culture medium
> Click the icon on the right-hand side of a culture media entry in
the culture media library list.
> The edit culture medium pop-up screen appears.
> Edit any information of the culture medium either in the details section
or the compounds section.
> Click the [SAVE] button at the bottom of the EDIT CULTURE
MEDIUM pop-up screen.
> The changed culture medium is saved and stored in the culture
media library list.
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6.4.3 Delete culture media
> Click the icon on the right-hand side of a culture medium entry
in the culture media library list.
> Confirm the deletion of the selected culture medium.
> The selected culture medium is deleted from the culture media library
list.
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6.5 Compounds
The compounds library lists all compounds that are available for the
creation of culture media. eve already provides a comprehensive
amount of standard culture media compounds However, in order to
access the culture media compounds library, proceed as follows:
> Click the [COMPOUNDS] button on the left-hand side of the
LIBRARIES menu.
> The compounds library list appears.
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6.5.1 Add new compound
To add a new compound to the culture media compounds library,
proceed as follows:
> Click the [ADD NEW COMPOUNDS] button at the top of the compounds
library list.
> The create compound pop-up screen appears.
> Enter a name for the compound (mandatory).
> Enter additional information into the optional fields.
Glucose monohydrate example:
> Click the [SAVE] button at the bottom of the CREATE
COMPOUND pop-up screen.
> The new compound is added to the compound library list.
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6.5.2 Edit compound
> Click the icon on the right-hand side of a compound entry in the
compound library list.
> The EDIT COMPOUND pop-up screen appears.
> Edit any information of the specific compound.
> Click the [SAVE] button at the bottom of the EDIT COMPOUND
pop-up screen.
> The changed compound is saved and stored in the compound library
list.
6.5.3 Delete compound
> Click the icon on the right-hand side of a compound entry in the
compound library list.
> Confirm the deletion of the selected compound.
> The selected compound is deleted from the compound library list.
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6.6 Soft-sensors
Soft-sensors are defined algorithms providing calculated values and
information which cannot be easily obtained from an in-process
sensor e.g. batch biomass prediction. In order to access library of
soft-sensors proceed as follows:
> Click the [SOFT-SENSORS] button on the left-hand side of the
LIBRARIES menu.
> The soft-sensors library list appears.
Number Function
1 Navigation button to access the next page
2 Add new soft-sensor to the library
3 Edit existing soft-sensor in the library
4 Delete soft-sensor from the library
6.6.1 Soft-sensor basics
The scripts are based on C# (C sharp) scripting language (CS-Script),
which has a compiler built into every windows computer. Standard
library classes and methods are used, where appropriate, along with
specific methods added relating to the bioreactor parameters. A syntax
checker provides help in creating usable scripts and notification
of the successful saving of a script is always provided.
Access properties of any input parameter:
Name Description
input1.Value
Current value of a parameter,
read only
input1.Setpoint
Current value of a parameter,
read only for inputs but writeable
for output parameters
input1.MinimalValue
Minimal value of a parameter,
read only
input1.MaximalValue
Maximal value of a parameter,
read only
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Access properties of any output parameter:
Name Description
output1.Value
Current value of a parameter,
read only
Existing process variables:
Name Type Description
process.ProcessTime TimeSpan
Gets the elapsed time of
the process.
process.TimeSinceInoculation TimeSpan
Gets the elapsed time of
the process since inocu-
lation.
process.CurrentPhaseName string
Gets the name of the current
phase.
process.CurrentPhaseTime TimeSpan
Gets the elapsed time of
the current phase
process.IsInoculated bool
Gets the value if the process
has been inoculated.
process.IsStopped bool
Gets the value if the process
has been stopped.
Boolean and mathematical operators:
Operator Description
&&  AND
|| ("pipe" character) OR
! NOT
+, -, /, *, <, >, >=, <=, = Classical mathematical operators
== Test equality
!= Test inequality
++ Increment variable values by one
-- Decrement variable values by one
% Modus or MOD
Math.exp Exponential function
Math.Log Natural log to base e
Math.Log10 Log to base 10 of a number
Math.Abs
Absolute value of a decimal or double
precision number
Math.Sin Sine of a number
Math.Cos Cosine of a number
Math.Pow Raises a number to a specific power
A full list is available at: https://msdn.microsoft.com/en-us/lib-
rary/system.math_methods(v=vs.110).aspx
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Decisions and loops:
Name Description
Simple IF (Then)
if(x Boolean y);
{
statement is executed if the conditions are
met e.g. output = 30;
}
Simple IF (Then) ELSE
if(x Boolean y);
{
statement is executed if the conditions are
met e.g. output =30;
}
else
{
statement which is executed if the condition
is false
}
Examples
Monitoring a setpoint of a parameter:
// Assuming the setpoint of temperature has to be mon-
itored:
// Input: temp
// Output: tempSP
tempSP.Value = temp.Setpoint;
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Basic Respiratory quotient (RQ) calculation:
// Inputs: ExitO2, ExitCO2, AirFlow
// Outputs: RQ, OUR, CER
//Culture volume
var Wvol = 1.0;
// Specific rate
var specRate = AirFlow.Value / Wvol;
// Carbon Dioxide Evolution Rate
CER.Value = (ExitCO2.Value - 0.038) * specRate;
// Oxygen Uptake Rate
OUR.Value = (20.95 - ExitO2.Value) * specRate;
// RQ Value
RQ.Value = CER.Value / OUR.Value;
Fore a more comprehensive description about the
scripting in eve®, please ask for the eve® scripting
guide.
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6.6.2 Add new soft-sensor
In order to add a new soft-sensor to the soft-sensor library proceed as
follows:
> Click the [ADD NEW SOFT-SENSOR] button at the top of the softsensor
library list.
> The create soft-sensor pop-up screen appears which has a predefined
work flow to create a new soft-sensor and starts with the
basic details of the soft-sensor.
> Enter a name for the soft-sensor (mandatory).
> Enter any description about the soft-sensor (optional).
> Click the [NEXT] button in order to define the mandatory inputs of
the soft-sensor.
> The predefined work flow continues with the input list.
> Click the [ADD INPUT] button at the top of the input list.
> A new input is added to the input list.
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> Click the input name field in order to change the name of a specific
input.
> Click the parameter type field in order to change the parameter
type of a specific input.
> The configured input appears in the input list.
> Click the [NEXT] button in order to define the outputs of the soft-
sensor.
> The predefined work flow continues with the output list.
> Click the [ADD OUTPUT] button at the top of the output list.
> A new output is added to the output list.
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> Click the output name field in order to change the name of a specific
output.
> Click the parameter type field in order to change the parameter
type of a specific output.
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> Click the unit field in order to change the unit of a specific output.
> The configured output appears in the output list.
> Click the [NEXT] button in order to define the constants of the soft-
sensor.
> The predefined work flow continues with the constants of the soft-
sensor.
> Click the [ADD CONSTANT] button at the top of the constant list.
> A new constant is added to the constant list.
> Click the constant name field in order to change the name of a specific
constant.
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> Click the unit field in order to change the unit of a specific con-
stant.
> Click the [NEXT] button in order to define the expression of the
soft-sensor.
> The predefined work flow continues with the expression of the
soft-sensor.
> Enter any script either directly into the box or copy/paste from a
text file.
> Any errors will be noticed and a description plus location of the
error will be given at the top of the script entry window. If no errors
appear the [SAVE] button is activated.
> Define the sampling time (calculation time) of the soft-sensor.
> Define the values of the constants.
> Click the [SAVE] button at the bottom of the CREATE SOFTSENSOR
pop-up screen.
> The soft-sensor is added to the soft-sensor library list.
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6.6.3 Apply soft-sensor
Soft-sensors can be applied to running and completed batch processes.
To use a soft-sensors in running and completed batch processes,
proceed as follows:
6.6.3.1 Running batch
To select a soft-sensor for your batch proceed as follows:
> Click the [SOFT SENSOR PARAMETERS] button in the CONFIGURE
PARAMETERS section.
> Click the [ADD] button at the top of the soft-sensor parameters list.
> A new window ADD A SOFT-SENSOR appears.
> Click on the soft-sensor from your soft-sensor library.
> Select which data should be used for the soft-sensor caluclation.
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> Click the edit button to change the constant values if necessary.
> Click the [OK]button to save your selected soft-sensorparameter.
> The selected soft-sensors are displayed in the parameter list.
6.6.3.2 Completed batch
> Open chart view of the completed batch (see " Monitor a batch
process" on page 74).
> The chart view of the batch appers.
> Click the [PARAMETERS] button at the bottom of the chart.
> The device parameter window appears.
> Click the [ADD SOFT-SENSOR] button at the top of the window.
> Select the soft-sensor from your soft-sensor library list.
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> The calculated parameters from the soft-sensor can now be displayed
in the chart view.
6.6.4 Edit a soft-sensor
Soft-sensors can be edited during the planing of a batch, in a batch
or in the soft-sensor library itself.
Edit a soft-sensor in the library:
> Click the icon on the right-hand side of a soft-sensor entry in the
soft-sensor library list.
> The edit soft-sensor pop-up screen appears.
> Edit any information of the specific soft-sensor in one of the sections
(details, input, outputs, constants or expression).
> Click the [SAVE] button at the bottom of the EDIT SOFT-SENSOR
pop-up screen.
> The changed soft-sensor is saved and stored in the soft-sensor library
list.
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Edit a soft-sensor in a planned batch:
As soon as the soft-sensor is selected from the soft-sensor library list
the specific outputs will be displayed as parameters:
> Click the icon on the right-hand side of a soft-sensor output.
> The EDIT SOFT-SENSOR pop-up screen appears where in the configuration
tab the soft-sensor can be edited (e.g. constants or
sampling time).
> Click the icon to redo the soft-senor input binding.
> The BIND SOFT-SENSOR INPUTS pop-up screen appears where
inputs can be binded to specific parameters.
6.6.5 Delete a soft-sensor
> Click the icon on the right-hand side of a soft-sensor entry in the
soft-sensor library list.
> Confirm the deletion of the selected soft-sensor.
> The selected soft-sensor is deleted from the soft-sensor library list.
If soft-sensors are applied on completed batches they
can be deleted as well directly from the parameter list.
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6.7 Audit trail
The audit trail logs every user management and batch related
actions of the users. The audit trail cannot be turned off due to security
reasons. In order to access the library of the audit trail proceed as
follows:
> Click the [AUDIT TRAIL] button on the left-hand side of the
LIBRARIES menu.
> The audit trail library appears.
The audit trail comprises entries in the following categories:
Name Description
EVENT TIME Timestamp of the logged action
USER
The user ID of the (logged) user responsible for this
action
EVENT TYPE What kind of action of the user has executed
ACTION Detailed description of the action (if required)
PROJECT Project associated to the action
EXPERIMENT Experiment associated to the action
BATCH Batch associated to the action
DEVICE Device associated to the action
IP ADDRESS
The address of the device, the action was
executed from (see "Global" on page 173)
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In the following list, all types of ACTIONS or EVENT TYPES allowed
in eve® are listed. For a more comprehensive description of each
audit trail entry, see the eve® functional specification document.
– AlarmActivated
– AlarmDeactivated
– AlarmRangeChange
– AlarmyTypeSwitch
– BackupCreated
– BasicSetupMetadataCreated
– BasicSetupMetadataDeleted
– BatchArchived
– BatchCreated
– BatchDeleted
– BatchEmailAlarmSubscribed
– BatchEmailAlarmUnsubscribed
– BatchUnarchived
– CommuncationLost
– CommunicationRestored
– ControlActivated
– ControlDeactivated
– CultureMediaMetadataCreated
– CultureMediaMetadataDeleted
– DefaultBatchReportSettingsEdited
– DeviceAdded
– DeviceAlarmAcknowledged
– DeviceNameChanged
– DeviceRemoved
– EmailServerActivated
– EmailServerChanged
– EmailServerDeactivated
– FunctionChanged
– FunctionCreate
– FunctionDelete
– FunctionStart
– FunctionStop
– Inoculate
– InstallerBackupDeleted
– LogOff
– LogOn
– LogOnFailed
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– LowerAlarmChange
– LowerAlarmRangeChange
– MultiExperimentCreated
– MultiExperimentDeleted
– OfflineParameterCreated
– OfflineParameterValueConfirmed
– OfflineParameterValueSubmitted
– OfflineSpampleTaken
– OrganismMetadataCreated
– OrganismMetadataDeleted
– ParameterAlarmAcknowledged
– ParameterAlarmInactive
– ParamererAlarmOutOfRange
– ParameterNameChanged
– PasswordChanged
– PhaseSkipped
– RecipeCreated
– RecipeDeleted
– RecipeExported
– RecipeImported
– RefreshRateChanged
– ReportGenerated
– RestApiActivated
– RestApiDeactivated
– RestoreFromBackup
– SetpointChange
– SettingsChaged
– SoftSensorAdded
– SoftSensorAddedInRunningBatch
– SoftSensorBindingChanged
– SoftSensorChanged
– SoftSensorRemoved
– Start
– Stop
– UpperAlarmChange
– UpperAlarmRangeChange
– UserActivated
– UserDeactivated
– UserEmailAlarmNotificationActivated
– UserEmailAlarmNotificationDeactivated
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– UserEmailChanged
– UserRoleChanged
6.7.1 Export
All audit trail entries can be exported. To export the audit trail entries
proceed as follows:
> Click the [EXPORT] button at the right bottom corner of the page.
> The AUDIT TRAIL REPORT pop-up screen appears.
In the AUDIT TRAIL REPORT audit trail entries from a specific time
period and/or a specific person, device, project etc. can be selected
(filtered).
The filter can be set to include only audit trail entries
containing a specific combination of signs or words
(upper and lower case sensitive; syntax symbols used:
+ – AND operation, |– OR operation, - – negates a
single token, “ – wraps a number of tokens to signify a
phrase for searching, * at the end of a term – prefix
query).
> Click the [PREVIEW ]button.
> The PREVIEW screen appears.
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> Click the download icon in order to download and save the report
of the selected audit trail entries.
> Select the appropriate file format (e.g. pdf).
> The report is saved and downloaded and can be open with any program
which can read pdf files.
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7 Resources
The RESOURCESmenu covers the binding of equipment and devices,
the creation of projects and user management. The following three
sub-menus are available:
– Equipment (see "Equipment" below)
– Projects (see "Projects" on page 162)
– User administration (see "User administration" on page 164)
In order to manage the resources proceed as follows:
> Click the [RESOURCES] button on the left-hand side of the screen.
> The sub-menus of RESOURCES appears.
7.1 Equipment
eve the bioprocess platform software embraces incubator shakers
and bioreactors of all sizes and types, and lets you connect and manage
your equipments in a convenient way.
You can access the overview of the connected equipment as fallows:
> Click the [EQUIPMENT] button on the left-hand side of the
RESOURCES menu.
> The overview page of the connected equipment appears.
If you connect an item of equipment to eve the first
time, the equipment overview is the landing page as
soon as you want to start a batch process.
7.1.1 Add new equipment
New equipment or upgrades can be added easily by the [ADD NEW
EQUIPMENT] button on the EQUIPMENT OVERVIEW page.
> Click the [ADD NEW EQUIPMENT] button on the top of the page.
> The ADD EQUIPMENT pop-up screen appears where the selection
of all different kinds of bioreactors and shakers is possible.
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7.1.1.1 Select equipment
To select a bioreactor or shaker simply click on one of the corresponding
icons which appear in the ADD EQUIPMENT pop-up
screen.
> Click on the icon of your corresponding equipment.
> You will be forwarded automatically to the CONFIGURATION step
of the ADD EQUIPMENT pop-up screen.
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7.1.1.2 Configure Equipment
The equipment configuration needs only three pieces of information
for a complete connection of all devices:
– Name of the equipment
– IP Address
– Port
In order to connect the equipment proceed as follows:
> Enter any name for the equipment.
> Enter the IP address of the specific equipment.
> Enter the corresponding port (usual 8080).
> Click the [SAVE] button at the bottom of the ADD EQUIPMENT
pop-up screen.
> The new equipment is added to the EQUIPMENT OVERVIEW.
The standard configuration for any INFORS
HT equipment communicates via port 8080.
7.1.1.3 Edit equipment
A connected equipment can be renamed and/or the refresh rate can
be changed. To rename the equipment and/or change the refresh
rate, proceed as follows:
> Click the settings icon on the right hand-side of the equipment
name.
> The EDIT EQUIPMENT pop-up screen appears.
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> The name and the refresh rate of the entire equipment can be
changed.
> Click the [SAVE] button at the bottom of the EDIT EQUIPMENT
pop-up screen.
By changing the refresh rate the entire communication
with the equipment is configured.
7.1.2 Bind device to existing equipment
External equipment such as balances, pumps or any other device
(e.g. HPLC, mass spectrometer etc.) can be linked to an existing
equipment (e.g. bioreactor).
To add an external device/equipment eve provides different drivers
for that, namely:
– Balances (Ohaus, Mettler Toledo, Kern, Sartorius)
– OPC (XML-DA and DA)
After successful installation of the driver the device can be added to
the eve equipment list (see "Add new equipment" on page 149). To
bind an external device/equipment to an existing one proceed as fol-
lows:
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> Click the [ADD NEW EQUIPMENT] button on the top of the page.
> The ADD EQUIPMENT pop-up screen appears where the selection
of all the equipment is possible.
> Select the external device/equipment (e.g. Ohaus Balance).
> Enter the IP address of the specific equipment (see "Configure
Equipment" on page 1).
> The external device appears in the equipment list.
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> Click the plus icon of any bioreactor vessel in order to open the
bind analytical device page.
> The bind analytical device page appers.
> The bind analytical device page appers.
> Select the external device/equipment by clicking the checkbox.
> Click the [OK] button to save the configuration.
> The selected external device/equipment is binded to the specific
bioreactor vessel. This linkage is displayed next to the type of the
external device.
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7.1.3 Delete device from existing equipment
To delete a linkage of an external device from an equipment proceed
as follows:
> Click the plus icon of the bioreactor vessel in order to open the bind
analytical device page.
> The bind analytical device page appears.
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7.1.4 Edit device
In order to edit the characteristics of a device, proceed as follows:
> Click directly on the device icon of the equipment.
> The overview screen of the devices is displayed.
Number Function
1 Device list of the specific vessel
2 Edit device parameter appearance
3 Edit device parameter settings
4 Sensor device
5 Actor device
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7.1.4.1 Additional parameter information
Some device parameters (e.g. pumps) can have several parameter
information. In order to display or hide the additional parameters, proceed
as follows:
> Click the icon at the top of a device.
> The device parameter appearance screen appears.
> Click on the parameters (check boxes) which should appear for the
specific device.
> Click the [SAVE] button at the bottom of the screen.
> The overview screen of the devices is displayed.
> Click directly on the icon of the changed device in order to see the
parameter appearance.
> The corresponding device parameters appear in hidden or visible
mode.
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7.1.4.2 Change device parameter settings
In the device parameter settings the name, unit, decimals and the
log precision can be configured. To change the settings of a device
parameter, proceed as follows:
> Click directly on the icon of the specific device.
> Click the icon next to the specific device parameter.
> The specific device parameter settings screen appears.
> To change any settings of the specific device parameter enter a
value into the field and click the [SAVE] button at the bottom of the
screen.
> The new settings are saved to this specific device parameter on the
specific equipment vessel.
The option decimals defines how many decimals are
displayed of the specific parameter.
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Log precision:
The log precision defines the number of decimals which are saved
into the database for the configured parameter. The system writes a
new value into the database as soon as a value is within the specified
decimals.
The number of decimals is by default 0 for each parameter.
The entered value for the decimals must be
between 0 and 8.
PID settings:
PID controllers (Proportional Integral Derivative controller) are used
for some of the parameters. The PID function is based on a generic
fomrula and where the individual terms can be described as:
– P = proportional factor, proportional response to an error, used to
reach a setpoint. The bigger the value, the sharper the control.
– I = integral factor, integration of the error in 1/second. A typical
integral factor is < 0.05.
– D = differential quotient, derviative of the error, set in seconds
(mostly to 0).
PID settings can be accessed in the device parameter settings and in
custom functions (see eve scripting guide).
term description
P (PTerm)
Proportional factor: The greater the discrepancy between the setpoint
value and the actual value the greater the controller output.
I (I Term, s-1)
The integral factor aggregates all errors over the time. If the setpoint
is not achieved using the proportional factor, the integral factor adjusts
the output successively until the setpoint value is achieved. An
integral factor set too high will lead to oscillation of the control loop.
D (DTerm, s)
The differential quotient calculates the change in the actual value
over the time and counteracts this change to limit any overshoot.
Negative Factor
The negative factor can be used to add weighting to two-sided control
(+100 to -100 %) (e.g. heavy acid, light alkali). In the process 1 is
the balance and 0.5 or 2 equate to the half or double the controller
output accordingly. Example: Nitrogen influences the pO 2 value less
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than oxygen, thus a negative factor of 2 can compensate for the reaction
of the controller.
Deadband
If a dead band is entered, no control is implemented within this value
at either side of the setpoint value (symmetrically, + / -). I.e. the controller
output is = 0. The dead band is used for pH control.
I Limit (Integ. Limit, %)
The integral influence is used to ensure that the integral factor cannot
increase over an indefinite period. This limits erroneous accumulation.
The integral influence is set between 0 and 100 % of the controller
output.
Evaluation Time
The evaluation time determines the intervals in seconds at which the
PID value is recalculated. The controller speed is defined this way. A
scanning time of 10 seconds is a good average value.
Inappropriate changes to the PID settings may have a
negative effect on the batch or the device. Only
change the PID settings, if you are fully aware of the
consequences or after consulting the manufacturer.
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7.1.4.3 Configure unknown device parameter
Some device parameters are not recognized instantly and marked
with a question mark. Such parameters have to be configured in
order to define the specific behaviour (i.e. actor/sensor, units, default
setpoints etc.).
> Click the icon next to the specific device parameter.
> The device parameter appearance screen appears.
> Select the device type from the dropdown list (e.g. GenericBalance
for any balance).
> The settings of the unknown parameter will be changed according
to the selected device type. If any specific settings need to be configured
(e.g. unit change, log precision rate etc.) please follow the
steps above (see "Change device parameter settings" on
page 158)
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7.2 Projects
Projects are the top level of the experiment structure. A project can
consist of several experiments with several batches.
> Click the [PROJECT] button on the left-hand side of the
RESOURCES menu.
> The PROJECT screen appears.
Number Function
1 Name (unique) of the project
2
Description of the project which cannot be longer
than 500 characters
3 Owner of the project
4 Number of planned experiments within the project
5 Add button for new project
6 Number of running experiments within the project
7
Number of completed experiments within
the project
8 Creation date of the project
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7.2.1 Create new project
> Click the [ADD NEW PROJECT] button at the bottom of
the project list.
> A pop-up screen appears where the new project can be defined.
> Enter a project name for the project.
> Enter a description to your project (optional).
> Click the [OK] button at the bottom of the pop-up screen.
> The project is created and listed in the project list.
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7.3 User administration
The user administration enables the organisation of users and their
dedicated user roles. The following five user roles are available:
– System administrator
– Manager
– User
– Technician
– Operator
– Guest
The system administrator has the right to add and edit an user, connect
equipment and activate licenses (IT role). The user has access to
all the features in eve® the bioprocess platform software except for
the above mentioned. The additional user roles are described in the
table below.
Equipment
management
User
administration
System
settings
License
management
Batch
configuration
Batch
execution
Batch
access
System
Administrator
x x x x
Manager x x x x x x x
User (x) x x x
Technician x x
Operator (x) x
Guest* x
x Full rights
(x) Partial rights
*Guest account has "read only" access and can expire after a certain time.
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To access the user administration proceed as follows.
> Click the [USER ADMINISTRATION] button on the left-hand side of
the RESOURCES menu.
> A list of all created users appears.
Number Function
1 Create new user
2 Edit existing user
3 Reset password for the user
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7.3.1 Add new user
> Click the add new user button at the top of the user list.
> A pop-up screen appears where the new user can be defined.
> Enter an unique username for the user.
> Enter a password for the user.
> Confirm the password by entering the same password a second
time.
> If not already selected, select the active option for this specific user
account.
> Specify the corresponding user role (e.g. system administrator or
user).
> Click the [OK] button at the bottom of the pop-up screen.
> The new user is created and listed in the user list.
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7.3.2 Edit user
> Click the on the right-hand side of an user account entry in the
user account list.
> The edit user pop-up screen appears.
7.3.2.1 Activate/inactivate user
eve allows you to inactivate an user profile. The complete deletion of
any user profile is not permitted. In order to activate or inactive an
user profile proceed as follows:
> Select or deselect the check box in order to activate or inactive an
user profile.
> Click the [OK] button at the bottom of the pop-up screen.
> The user profile is activated or deactivated.
7.3.2.2 Define user role
> Select the corresponding user role for the specific user profile.
> Click the [OK] button at the bottom of the pop-up screen.
> The user profile gets the corresponding user role.
7.3.2.3 Reset password
> Click the reset [PASSWORD BUTTON] on the right-hand side of an
user account.
> The edit password pop-up screen appears.
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> Enter the new password for the specific user account.
> Confirm the new password for the specific user account.
> Click the [OK] button at the bottom of the pop-up screen.
> The new password is saved.
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8 Settings
In the SETTINGS menu user specific and global options are available
which enables the customisation of each user. The following four
sub-menus are available:
– My profile settings (see "My Profile" below)
– Gloabal settings (see "Global" on page 173)
– Licenses (see "License" on page 188)
– About (see "About" on page 187)
In order to access the settings proceed as follows:
> Click the [SETTINGS] button on the left-hand side of the screen.
> The sub-menus of SETTINGS appear.
8.1 My Profile
In the MY PROFILE menu you can change the password and profile
picture of your account.
> Click the [MY PROFILE] button on the left-hand side of the
SETTINGS menu.
> The MY PROFILE screen appears.
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8.1.1 Change Password
> Click the [CHANGE PASSWORD] button.
> The CHANGE PASSWORD pop-up screen appears.
> Enter the old and new passwords in the corresponding fields.
> Confirm the new password by entering it in the corresponding
field.
> Click the[SAVE] button at the bottom of the pop-up screen.
> The new password has been saved successfully.
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8.1.2 Change profile picture
> Click the icon to change the profile picture.
> The SELECT AND CROP PICTURE pop-up screen appears.
> Click the [BROWSE COMPUTER] button at the bottom right-hand
side of the pop-up screen.
> The explorer window of your operating system appears.
> Select your preferred profile picture.
> Select the specific area of your profile picture by dragging the
square with the mouse.
> Click the [OK] button in order to save the cropped profile picture.
> The profile picture has been saved successfully.
If you want to delete a profile picture click the
icon and the profile picture will be deleted per-
manently.
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8.1.3 Enable e-mail alarm notification
As soon as the e-mail server is configured correctly (see "Global" on
the next page) the e-mail address can be specified and the notification
can be enabled.
> Enter a valid e-mail address to received e-mail alarm notifications.
> Click the toggle button to enable the e-mail notification.
> The e-mail alarm notification is enabled for the specified e-mail
address.
By default, any batch process that is started with the
user that enabled the e-mail alarm notification is subscribed
to the self-created batches.
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8.2 Global
In the GLOBAL settings menu, the System administrator e-mail
address, the data export behaviour, system settings and backups can
be configured. Furthermore, settings of user and password/security
behaviour can be configured, as well as the default report settings
and custom meta data:
– Default batch report settings (see "Default batch report settings"
on the facing page)
– Mandatory batch information (see "Mandatory information" on
page 175)
– Automatic user log-off (see "Automatic user log-off" on page 184)
– User blocking after failed log-in (see "User blocking after failed
log-in" on page 184)
– Password aging (see "Password aging" on page 185)
– IP white-list (see "IP white-list" on page 185)
– High security password (see "High security password" on
page 185)
– Custom metadata (see "Data settings" on page 176)
> Click the [GLOBAL] button on the left-hand side of the SETTINGS
menu.
> The GLOBAL screen appears.
Depending on your active packages, features of the
batch, system and data settings can not be configured.
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8.2.1 Batch settings
8.2.1.1 Default batch report settings
To configure a default batch report setting (applies to the entire eve
system) proceed as follows:
> Click the [EDIT] button next to the default batch report settings.
> The batch report settings wizard appears.
> Select all component check boxes which should be selected by
default for every report.
> Click the [NEXT] button.
> The parameter option screen appears.
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> Select all parameter option check boxes which should be selected
by default for all parameter.
> Click the [SAVE] button to save your default batch reporting set-
tings.
> The default batch report settings are applied to the entire eve sys-
tem.
8.2.1.2 Mandatory information
Mandatory culture media and organism information can be selected
to force the users to select at least one culture media and/or organism.
To configure that the user must select the information during the
planning of batch proceed as follows:
> Select the MANDATORY CULTURE MEDIA/ORGANISM option.
> The settings of the mandatory information are saved.
If a user wants to start a batch without filling out the
necessary information the execution is blocked.
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8.2.2 Data settings
8.2.2.1 .csv export settings
If you export recorded batch data as .csv two different export options
are available. By default, the exported batch data for a specific parameter
(e.g. temperature, °C) are only recorded when a change in
the signal value is measurable. This can lead to gaps in the parameter
time series (see example below).
If you want to change the behaviour of data export and fill the data
gaps, proceed as follows:
> Select the fill gaps in csv export option.
> The settings for the csv export are saved.
As an example, the table below shows how the two different
settings influence the csv data export of temperature
time series.
process time
h
no fill of gaps in csv
temperature, °C
fill of gaps in csv
temperature, °C
0 29.37 29.37
0.2 29.62 29.62
0.4 29.90 29.90
0.6 30.03 30.03
0.8 30.03
1.0 30.03
1.2 30.03
1.4 30.03
1.6 30.03
1.8 31.21 31.21
2.0 31.19 31.19
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8.2.2.2 Custom basic setup metadata
Custom basic setup metadata can be created to extend the information
of the basic setup in planning a batch. If you want to add,
change or delete any of the basic setup metadata, proceed as fol-
lows:
> Click the [EDIT] button next to the configure basic setup metadata
settings.
> The CONFIGURE BASIC SETUP METADATA pop-up screen
appears.
> Click the [ADD] button to add a new metadata information.
> A new line is added to the custom organism metadata list.
> Enter the name of your metadata information and select the units.
Click the [SAVE] button in order to save your configuration.
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If you do not select a unit and the metadeta information
has "select" under unit, the unit will be not
required for entering the information.
The custom basic setup metadata are available in the
organism library as soon as a new organism is created
or an existing one is edited (see "Add new organism"
on page 120)
8.2.2.3 Custom organism metadata
Custom organism metadata can be created to extend the information
of the organisms in the organism library (see "Add new organism"
on page 120). If you want to add, change or delete any of the
organism metadata, proceed as follows:
> Click the [EDIT] button next to the configure organism metadata
settings.
> The CONFIGURE ORGANISM METADATA pop-up screen appears.
> Click the [ADD] button to add a new metadata information.
> A new line is added to the custom organism metadata list.
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> Enter the name of your metadata information and select the units.
Click the [SAVE] button in order to save your configuration.
If you do not select a unit and the metadata information
has "select" under unit, the unit will be not
required for entering the information.
The custom organism metadata are available in the
organism library as soon as a new organism is created
or an existing one is edited (see "Add new organism"
on page 120)
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8.2.2.4 Custom culture media metadata
Custom culture media metadata can be created to extend the information
of the culture media in the culture media library (see "Culture
media" on page 123). If you want to add, change or delete any of
the culture media metadata, proceed as follows:
> Click the [EDIT] button next to the configure culture media
metadata settings.
> The CONFIGURE CUTLURE MEDIA METADATA pop-up screen
appears.
> Click the [ADD] button to add a new metadata information.
> A new line is added to the custom organism metadata list.
> Enter the name of your metadata information and select the units.
Click the [SAVE] button in order to save your configuration.
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If you do not select a unit and the metadeta information
has "select" under unit, the unit will be not
required for entering the information.
The custom culture media metadata are available in
the culture media library as soon as a new culture
media is created or an existing one is edited (see "Culture
media" on page 123)
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8.2.2.5 Backup
eve® allows to create backups of the database with all entered and
acquired data at a specific date and time. The backup can be used to
restore the database at a later time. This action will overwrite all the
data in the database at the moment of restoring.
Create backup:
> Click the [CREATE BACKUP] button.
> A backup has been created and is stored under C:\INFORSHT\Backup.
The file name consists of the date and time of the
backup in the following format: yyyymmdd-hhmmss.
The system allows you to create backups of the complete
database at any date and time if no batch is run-
ning.
Change backup path:
By default the backup (and restore) path (see "Restore backup:"
below) is set to C:\INFORS-HT\backup. However, the location of the
backup can be changed manually to a local or network drive. To
change the location of the backup proceed as follows:
> Click into the BACKUP LOCATION Field and enter the path of the
backup location (e.g. C:\MyeveBackup).
> The new patch is saved automatically and will be used for further
backups.
When a new backup path is entered the corresponding
path and folder must be available.
Restore backup:
> Click the [RESTORE] button.
> The SELECT BACKUP TO RESTORE pop-up screen appears.
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> Select the backup file from the dropdown list.
> Click the [RESTORE]button at the bottom of the pop-up screen.
> Confirm the action by clicking the [OK] button.
> The backup has been restored.
Restore a backup will erase all current data in the database
and replaced by the backup data. This cannot be
undone!
Backup cleanup:
Due to safety reasons eve creates an automatic backup during the
update of the system. This backups can occupy large volume of disk
space. To delete and clean this backups proceed as follows:
> Click the [CLEANUP] button.
> Click the [OKAY] button to verify your cleanup.
> The automatic backups are now deleted from the drive.
Using the cleanup function does not influence the
backups that were created manually.
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8.2.3 System settings
8.2.3.1 Automatic user log-off
Activation of automatic user log-off allows the automatic log-off of
any user after a configurable period of inactivity. With this feature
activated, every user is logged off from the software after a defined
time of inactivity.
> Select the activate automatic user log-off check box.
> Enter a time interval in seconds for the automatic log-off.
> The settings for the automatic user log-off are saved.
8.2.3.2 User blocking after failed log-in
Activation of complete lockout from the software after a configurable
number of failed logins represents another password security
feature. Any attempt to use the software in an unauthorized
manner by guessing the password of a user using iterative procedures
is prevented by this feature. Once a user has been locked
out, a system administrator or a manager can activate the user
account again.
> Select the activate blocking user after failed log-in check box.
> Enter the number of failed log-in before blocking the user.
> The settings for the user blocking are saved.
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8.2.3.3 IP white-list
It is possible to control from which device(s) changes within a batch
can be executed via the restriction to specific IP addresses. If this feature
is activated no device with an IP address other than those on the
white list can be used to make any change within the batch.
> Select the restrict batch changes to IP addresses check box.
> Enter the IP adresses which are not restricted to batch changes
(e.g. 192.168.1.15; 192.168.1.16).
> The settings for the high security password are saved.
8.2.3.4 Password aging
For the security of passwords, it is important to control that users are
regularly changing their password. If activated, the PASSWORD
AGING feature ensures that the users are forced to change their passwords
after a configurable period.
> Select the password aging check box.
> Enter the expiration time in days.
> The settings for the password aging are saved.
8.2.3.5 High security password
With the activation of the feature HIGH SECURITY PASSWORD the
requirements concerning password security according to current
standards are met. Each new password created after activation of
the feature has to be composed of:
– At least 8 characters
– At least one upper and one lower case character
– At least one non-alpha numerical or numerical character
> Select the high security password check box.
> The new passwords will need high security characters.
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> The settings for the high security password are saved.
8.2.3.6 E-Mail server configuration
To configure the e-mail server (e-mail alarm notification) the configuration
setup should be tested first. After successful testing, each
user can enable the e-mail notification by themself and subscribe to
specific batches (see "Alarms" on page 75)
To configure the e-mail server proceed as follows:
> Select the configure e-mail server check box.
> The [EDIT] button of the e-mail server is activate.
> Click the [EDIT] button to setup the e-mail server configuration.
> The CONFIGURE E-MAIL SERVER pop-up screen appears
> Enter the host of your e-mail server (e.g. outlook.office365.com)
> Enter your e-mail user account credentials and the password.
> Enter a valid e-mail address to test the entered settings.
> Click the [SEND] button.
> After successful configuration an e-mail is sent to the specified
address.
Please consider the General Data
Protection Regulation (GDPR) according to the EU law
when entering personal data (i.e. e-mail address).
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8.2.3.7 REST API access
The REST API option activates an interface that allows to exchange
data with other softwares (e.g. Matlab or LIMS). Further information
about the REST API documentation can be found directly in the software
(info button).
To activate the REST API, proceed as follows:
> Select the enable 3rd party software access to eve (REST API)
checkbox.
> The [REST API] access is activate. Further information about the
interface can be found when clicking on the [INFO] button.
8.3 About
The ABOUT menu displays the current version of eve the bioprocess
platform software and further contact information of INFORS HT. eve
the bioprocess platform software is a member of the "swiss made
software" product family.
Whenever you have to contact any service and support
please specify the eve version.
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8.4 License
The license settings enables the activation of packages (i.e. additional
features), devices and the Software Support & Assurance
option. Each package can be activated by an activation ID which
either can be perpetual or subscription. After a successful purchase
of eve or any additional package of eve you will receive an activation
ID for the corresponding license.
To activate your licenses proceed as follows.
> Click the [LICENSE] button on the left-hand side of the SETTINGS
menu.
> The license screen appears.
Number Function
1 Add new license (activation ID) to the system
2 Type of license
3 Type of pricing mode and amount of days left
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8.4.1 Activate license online
> Click the [ADD NEW LICENSE] button at the top of the screen.
> The LICENSE ACTIVATION pop-up screen appears.
> Enter the activation ID from the e-mail or pdf-file (copy and paste)
into the enter activation ID field.
> Click the [NEXT]button at the bottom of the pop-up screen.
> The activation ID is validated automatically by the system.
> Follow the procedure in the license activation pop-up screen.
> After the logout the new features will be activated with the next
login.
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8.4.2 Activate license offline
> Click the [ADD NEW LICENSE] button at the top of the screen.
> The LICENSE ACTIVATION pop-up screen appears.
> Enter the activation ID from the e-mail or pdf-file (copy and paste)
into the enter activation ID filed.
> Click the [NEXT]button at the bottom of the pop-up screen.
> The activation ID is validated automatically by the system.
> Follow the procedure in the license activation pop-up screen.
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> Send the file that has been created to licensing@infors-ht.com.
> As soon as you receive the validated file you can continue with the
offline activation process.
> Click the [ACTIVATION] button at the bottom of the LICENSE
screen.
> The PENDING LICENSE ACTIVATION pop-up screen appears.
> Click either the [BROWSE COMPUTER] button and select the
received file from the e-mail or drag and drop the received file directly
into the pop-up screen.
> The selected file appears in the PENDING LICENSE ACTIVATION
pop-up screen.
> Click the activate button at the bottom of the PENDING LICENSE
ACTIVATION pop-up screen.
> After the logout the new features will be activated with the next
login.
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A
Alarm
Alarms are caused by parameter values
that are out of scope. The scope is defined
by upper and lower critical values which
can be fixed or dynamic.
Audit Trail
Logs every user action and allows a complete
record of what was done (e.g. create,
start and stop batch, log-in, log-out etc.)
B
Batch
An individual bioprocess configured in an
experiment and assigned to a bioreactor.
Batch strategy
Batch strategy is a series of defined phases
intend to provide process control, including
changing and shifiting setpoints of process
parameters, transition conditions and func-
tions.
C
Chart
Chart display enables to visualise and monitor
a batch process and its parameters.
Compounds
Ingredients of culture media (e.g. NaCl,
KOH etc.)
D
Device
A device delivers data to the software and
can be a measurement or control element
of an individual bioreactor (or peripheral
instrument assigned to that bioreactor) or
the bioreactor/shaker itself.
Device type
Defines the type of the device (e.g. PT100,
Stirre, pO2, etc.)
E
Equipment
A hypernym for all connected devices.
Experiment
The configuration of planned processes to
which individual batches can be assigned
and run.
F
Function
A function is an algorithm that can be
either in a phase or a soft-sensor and which
defines its behaviour.
I
Input
Input variables (also called independent
variable) are the inputs to functions and
can be any rational number.
L
Licenses
Can be activated by activation IDs and
enable the activation of additional pack-
ages.
O
Offline parameter
An offline parameter is a parameter which
value is delivered by a device that is not
connected to the software. The value is
therefor entered manually by the user.
9 Glossary
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Operating manual eve® | version 1.102 193
Output
Output variables (also called dependent
variable) are the output of functions and
can be any rational number.
P
Packages
Packages consist of certain features (e.g.
batch comparision in plan and control) and
can be added anytime.
Parameter
A discrete measured and/or controlled element
of a batch process e.g. temperature
°C.
Parameter type
The parameter type represents a device
and can generate multiple parameters
(e.g. Exit Gas analyser which generates the
parameter Offgas O2 and Offgas CO2)
Phase
A phase is a parameter dependent process
condition and consists of functions (1 function
per parameter, consecutive phases can
have the same function for the same para-
meter).
Project
A project is a higher-ranked description/acronym
of the main goal/product and
can consist of several experiments.
Q
Quickstart
A function which allows the user to start a
batch process quickly without any
advanced configuration.
R
Recipe
A user-defined configuration of a process
involving elements such as bioreactor,
organism, culture medium, set points,
phases and control strategies etc.
Refresh rate
Defines how often the system updates the
data on the chart display.
S
Setpoint
The target value which the control system
will aim to reach.
Soft-sensor
A soft sensor is a common name for software
sensor where several measurements
are processed together. The signals are
used for calculating additional actual process
information based on a specific
algorithm. It is used for process monitoring,
fault diagnosis, as well as control applic-
ations.
T
Transition
Phase transition defines the transition from
one phase to the other. It can have several
conditions (e.g. time-dependent, para-
meter/signal-dependent).
© Infors AG 2019. All rights reserved. eve® is a registered
trademark of Infors AG. More information about the product at
www.infors-ht.com/eve 
Infors AG
Rittergasse 27
CH-4013 Bottmingen
www.infors-ht.com
info@infors-ht.com