C8953
NMR structural analysis - seminar
2D NMR spectra, COSY
Jan Novotny
176003@mail.muni.cz
March 15, 2023
1
H NMR spectrum of naringenine in d6-acetone
COSY : β-cyclodextrine
5.0
5.0
4.5
4.5
4.0
4.0
3.5
3.5
δ2 - 1H (ppm)
5.0 5.0
4.5 4.5
4.0 4.0
3.5 3.5
δ1-1H(ppm)
Phase sensitive COSY
▶ direct vs. indirect dimension
▶ active coupling - antiphase crosspeak, passive coupling -
in-phase
1D 1
H of Atropine in DMSO
COSY : Atropine
8
8
6
6
4
4
2
2
8 8
6 6
4 4
2 2
δ1-1H(ppm)
COSY : Atropine
5
5
4
4
3
3
2
2
1
1
1
5 5
4 4
3 3
2 2
δ1-1
H(ppm)
Chinin - 1D 1
H
COSY - Chinin
10
10
8
8
6
6
4
4
2
2
δ2 - 1H (ppm)
8 8
6 6
4 4
2 2
δ1-1H(ppm)
5
10
6
9
7
8
3
2
4
N
1
O
CH3
22
11
12
OH
17
16
N
13
15
14
18
19
20
21
COSY - Chinin10 8 6 4 2
8 8
6 6
4 4
2 2
δ1-1
H(ppm)
5
10
6
9
7
8
3
2
4
N
1
O
CH3
22
11
12
OH
17
16
N
13
15
14
18
19
20
21
Next topic
1H-1H through space correlations (NOESY, ROESY)