Running test in CG990 subject

: separation of proteins


version 01

name
date


1)        Which two amino acids are responsible for negative charge of proteins?

a) glutamate, aspartate

b) tyrosine, alanine

c) arginine, lysine

d) glutamine, asparagine


2)        Hypothetical protein with relative molecular weight 30 kDa and pI = 9.0 is going to be
found after 2D GE (strip 3-10, vertical 10%T SDS-PAGE) in the gel:

a) at the bottom edge, between left and right lower quadrants

b) in upper left quadrant

c) in lower right quadrant

d) the protein will not be in the gel


3)        What does it mean partial separation?



















Running test in CG980 subject

: separation of proteins


version 02

name
date


1)        Using which method, we check the protein preparation yield?

a) nuclear magnetic resonance

b) mass spectrometry

c) denaturing gel electrophoresis

d) liquid chromatography


2)        Series of samples enabling a result accuracy evaluation are?

a) technical replicates

b) methodical replicates

c) experimental (biological) replicates

d) error replicates


3)        What is the importance of so called peak capacity?

















Running test in CG980 subject

: separation of proteins


version 03

name
date


1)        Which methods are used to separate proteins according to charge?

a) ion-pairing chromatography on reversed phases (IP-RPLC)

b) chiral chromatography

c) hydrophobic interactions chromatography

d) ion-exchange chromatography


2)        What is the purpose of separation in the first dimension of two-
          dimensional gel electrophoresis (2D GE)?

a) separation of proteins according to their molecular mass

b) separation of proteins according to their isoelectric point

c) pre-separation to achieve high final peak capacity

d) pre-separation to achieve high final resolution


3)        What is the protein equaliser?

















Running test in CG980 subject

: separation of proteins


version 04

name
date


1)        In a frame of separation methods, what does it mean complete separation?

a) separation of a mixture down to individual proteins

b) separation of a mixture down to protein classes according to chosen property

c) separating a group of proteins from mixture

d) selective separation of one protein from mixture


2)        What does not belong among possible inputs of protein separation?

a) whole-cell lysate

b) mixture of proteolytic products of protein cleavage

c) protein mixture after recombinant protein expression

d) decantate after precipitation and centrifugation of proteins


3)        What is the importance of multidimensional separations?